CrneC.00483.a

Glutamate 5-kinase, variant

CENTER ID: CrneC.00483.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.00483.a.B2.GE41250 Truncation 1 1 275
CrneC.00483.a.B3.GE41251 Truncation 2 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_05386
UniProt: T2BQ16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLKERGKGL HQKQALAAVG QGRLIALWDN LFSQLDQPIA QILLTRMDIS DRTRYLNAQN TFSELLQMGV IPIVNENDTV SVSEIKFGDN DTLSAIASAI CHADYLFLLT DVDCLYTDNP RTNPDAKPVR VVRDIEKVKQ QVSTSTLGTS LGTGGMSTKL IAAELATAAG TTTVVMHSSN VIDIFKVIEN GPSPCREVAE TPALFEGPLC TMFLRRESAL KDRKWWIAHG LHAAGTVVID EGACRAIRRK ESGGRLLPAG VVRVAGPFAS HQAVRLVVRR RRHDDVAAFS SIDESTPGSP TPDLSQVPQP ATLASPALRH LTIGTTNPIT IMSDTNNTEP DTPQLQPSMS LSSSIASLDP LSRSVPPSPA ITALAERLGT TSLTRIGRGV TEGESDEWEE VEVAKGLAQY NSVEIDRMKG RKSSEIEKLI GYVESEHVVD SITFL
NT Sequence
atggatctca aggaaagagg gaagggattg catcagaagc aggctcttgc tgctgttggt caagggcggt tgattgcgct gtgggataat ctgttctctc aattggatca gcctattgcg cagatccttc tcacccgaat ggacatttca gacagaacac gttatctcaa cgctcaaaac actttctccg aattattgca aatgggcgtt attcctatcg tcaatgagaa tgatacggtc tcagtctctg aaatcaaatt tggcgacaat gacacattat cggcgatcgc ctctgctatt tgccatgccg attatctctt tcttttgact gacgttgact gtttatacac agacaacccc cgtaccaacc ccgatgccaa acctgtgaga gtcgtacgcg atattgagaa ggtgaaacag caagtctcta cctctaccct cggaacatct ctcggcacag gaggcatgtc caccaagctc attgccgccg agcttgctac tgctgctggc acaaccaccg ttgttatgca ttcttctaac gtgatcgaca ttttcaaagt cattgaaaac ggcccttcgc cttgccgaga ggtcgcagag accccagcat tgtttgaggg cccgctgtgt acgatgtttt tgagacgaga gtctgccctt aaagaccgaa aatggtggat cgcccacggt cttcacgctg ctggtaccgt tgtcatcgac gaaggtgcct gccgtgccat ccgacgcaaa gaatccgggg gtcgtctcct tcctgctggg gtagtccgcg tggctggccc tttcgcaagt caccaagccg tccgtctcgt tgttcgtcgt cgacgccacg acgacgtcgc cgcctttagc tcaatcgacg aatcaacccc cggtagccct acccccgacc tttcccaagt accccagccg gcgacactcg cttctccggc tcttcgacat cttacgattg ggaccaccaa tccgatcacc atcatgtctg acaccaacaa cactgaacct gatactcctc aactccaacc ttccatgtct ctttcatctt caatcgcttc actcgatcca ttaagcagga gcgtgccccc tagtcctgcg ataacggctt tggcggagag gctgggcaca acatcgttga cgagaattgg gcgcggagtg accgaaggag agagtgatga atgggaagag gtcgaggtgg cgaagggttt ggcccagtac aacagtgtag aaattgacag gatgaaggga cgaaagagtt cggaaattga aaaacttatt gggtacgtcg agagcgagca tgtggtggac tcgattacat tcttgtag
Details for CrneC.00483.a.B2.GE41250
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD LKERGKGLHQ KQALAAVGQG RLIALWDNLF SQLDQPIAQI LLTRMDISDR TRYLNAQNTF SELLQMGVIP IVNENDTVSV SEIKFGDNDT LSAIASAICH ADYLFLLTDV DCLYTDNPRT NPDAKPVRVV RDIEKVKQQV STSTLGTSLG TGGMSTKLIA AELATAAGTT TVVMHSSNVI DIFKVIENGP SPCREVAETP ALFEGPLCTM FLRRESALKD RKWWIAHGLH AAGTVVIDEG ACRAIRRKES GGRLLPAGVV RVAGPXXSHQ AVR
Validated NT Sequence
atggctcacc accaccacca ccatatggat ctcaaggaaa gagggaaggg attgcatcag aagcaggctc ttgctgctgt tggtcaaggg cggttgattg cgctgtggga taatctgttc tctcaattgg atcagcctat tgcgcagatc cttctcaccc gaatggacat ttcagacaga acacgttatc tcaacgctca aaacactttc tccgaattat tgcaaatggg cgttattcct atcgtcaatg agaatgatac ggtctcagtc tctgaaatca aatttggcga caatgacaca ttatcggcga tcgcctctgc tatttgccat gccgattatc tctttctttt gactgacgtt gactgtttat acacagacaa cccccgtacc aaccccgatg ccaaacctgt gagagtcgta cgcgatattg agaaggtgaa acagcaagtc tctacctcta ccctcggaac atctctcggc acaggaggca tgtccaccaa gctcattgcc gccgagcttg ctactgctgc tggcacaacc accgttgtta tgcattcttc taacgtgatc gacattttca aagtcattga aaacggccct tcgccttgcc gagaggtcgc agagacccca gcattgtttg agggcccgct gtgtacgatg tttttgagac gagagtctgc ccttaaagac cgaaaatggt ggatcgccca cggtcttcac gctgctggta ccgttgtcat cgacgaaggt gcctgccgtg ccatccgacg caaagaatcc gggggtcgtc tccttcctgc tggggtagtc cgcgtggctg gcccttnnnc aagtcaccaa gccgtccgtt aagtgagtaa gat
Expected Protein Sequence
MAHHHHHHMD LKERGKGLHQ KQALAAVGQG RLIALWDNLF SQLDQPIAQI LLTRMDISDR TRYLNAQNTF SELLQMGVIP IVNENDTVSV SEIKFGDNDT LSAIASAICH ADYLFLLTDV DCLYTDNPRT NPDAKPVRVV RDIEKVKQQV STSTLGTSLG TGGMSTKLIA AELATAAGTT TVVMHSSNVI DIFKVIENGP SPCREVAETP ALFEGPLCTM FLRRESALKD RKWWIAHGLH AAGTVVIDEG ACRAIRRKES GGRLLPAGVV RVAGPFASHQ AVR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tcaaggaaag agggaaggga ttgcatcaga agcaggctct tgctgctgtt ggtcaagggc ggttgattgc gctgtgggat aatctgttct ctcaattgga tcagcctatt gcgcagatcc ttctcacccg aatggacatt tcagacagaa cacgttatct caacgctcaa aacactttct ccgaattatt gcaaatgggc gttattccta tcgtcaatga gaatgatacg gtctcagtct ctgaaatcaa atttggcgac aatgacacat tatcggcgat cgcctctgct atttgccatg ccgattatct ctttcttttg actgacgttg actgtttata cacagacaac ccccgtacca accccgatgc caaacctgtg agagtcgtac gcgatattga gaaggtgaaa cagcaagtct ctacctctac cctcggaaca tctctcggca caggaggcat gtccaccaag ctcattgccg ccgagcttgc tactgctgct ggcacaacca ccgttgttat gcattcttct aacgtgatcg acattttcaa agtcattgaa aacggccctt cgccttgccg agaggtcgca gagaccccag cattgtttga gggcccgctg tgtacgatgt ttttgagacg agagtctgcc cttaaagacc gaaaatggtg gatcgcccac ggtcttcacg ctgctggtac cgttgtcatc gacgaaggtg cctgccgtgc catccgacgc aaagaatccg ggggtcgtct ccttcctgct ggggtagtcc gcgtggctgg ccctttcgca agtcaccaag ccgtccgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for CrneC.00483.a.B3.GE41251
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMD LKERGKGLHQ KQALAAVGQG RLIALWDNLF SQLDQPIAQI LLTRMDISDR TRYLNAQNTF SELLQMGVIP IVNENDTVSV SEIKFGDNDT LSAIASAICH ADYLFLLTDV DCLYTDNPRT NPDAKPVRVV RDIEKVKQQV STSTLGTSLG TGGMSTKLIA AELATAAGTT TVVMHSSNVI DIFKVIENGP SPCREVAETP ALFEGPLCTM FLRRESALKD RKWWIAHGLH AAGTVVIDEG ACRAIRRKES GGRLLPAGVV RVAGPFASHQ AVRLVVRRRR HDDVAAFSSI DESTPGSPTP DLSQVPQPAT LAS
Validated NT Sequence
atataccatg gctcaccacc accaccacca tatggatctc aaggaaagag ggaagggatt gcatcagaag caggctcttg ctgctgttgg tcaagggcgg ttgattgcgc tgtgggataa tctgttctct caattggatc agcctattgc gcagatcctt ctcacccgaa tggacatttc agacagaaca cgttatctca acgctcaaaa cactttctcc gaattattgc aaatgggcgt tattcctatc gtcaatgaga atgatacggt ctcagtctct gaaatcaaat ttggcgacaa tgacacatta tcggcgatcg cctctgctat ttgccatgcc gattatctct ttcttttgac tgacgttgac tgtttataca cagacaaccc ccgtaccaac cccgatgcca aacctgtgag agtcgtacgc gatattgaga aggtgaaaca gcaagtctct acctctaccc tcggaacatc tctcggcaca ggaggcatgt ccaccaagct cattgccgcc gagcttgcta ctgctgctgg cacaaccacc gttgttatgc attcttctaa cgtgatcgac attttcaaag tcattgaaaa cggcccttcg ccttgccgag aggtcgcaga gaccccagca ttgtttgagg gcccgctgtg tacgatgttt ttgagacgag agtctgccct taaagaccga aaatggtgga tcgcccacgg tcttcacgct gctggtaccg ttgtcatcga cgaaggtgcc tgccgtgcca tccgacgcaa agaatccggg ggtcgtctcc ttcctgctgg ggtagtccgc gtggctggcc ctttcgcaag tcaccaagcc gtccgtctcg ttgttcgtcg tcgacgccac gacgacgtcg ccgcctttag ctcaatcgac gaatcaaccc ccggtagccc tacccccgac ctttcccaag taccccagcc ggcgacactc gcttctcc
Expected Protein Sequence
MAHHHHHHMD LKERGKGLHQ KQALAAVGQG RLIALWDNLF SQLDQPIAQI LLTRMDISDR TRYLNAQNTF SELLQMGVIP IVNENDTVSV SEIKFGDNDT LSAIASAICH ADYLFLLTDV DCLYTDNPRT NPDAKPVRVV RDIEKVKQQV STSTLGTSLG TGGMSTKLIA AELATAAGTT TVVMHSSNVI DIFKVIENGP SPCREVAETP ALFEGPLCTM FLRRESALKD RKWWIAHGLH AAGTVVIDEG ACRAIRRKES GGRLLPAGVV RVAGPFASHQ AVRLVVRRRR HDDVAAFSSI DESTPGSPTP DLSQVPQPAT LASPALRHLT IG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tcaaggaaag agggaaggga ttgcatcaga agcaggctct tgctgctgtt ggtcaagggc ggttgattgc gctgtgggat aatctgttct ctcaattgga tcagcctatt gcgcagatcc ttctcacccg aatggacatt tcagacagaa cacgttatct caacgctcaa aacactttct ccgaattatt gcaaatgggc gttattccta tcgtcaatga gaatgatacg gtctcagtct ctgaaatcaa atttggcgac aatgacacat tatcggcgat cgcctctgct atttgccatg ccgattatct ctttcttttg actgacgttg actgtttata cacagacaac ccccgtacca accccgatgc caaacctgtg agagtcgtac gcgatattga gaaggtgaaa cagcaagtct ctacctctac cctcggaaca tctctcggca caggaggcat gtccaccaag ctcattgccg ccgagcttgc tactgctgct ggcacaacca ccgttgttat gcattcttct aacgtgatcg acattttcaa agtcattgaa aacggccctt cgccttgccg agaggtcgca gagaccccag cattgtttga gggcccgctg tgtacgatgt ttttgagacg agagtctgcc cttaaagacc gaaaatggtg gatcgcccac ggtcttcacg ctgctggtac cgttgtcatc gacgaaggtg cctgccgtgc catccgacgc aaagaatccg ggggtcgtct ccttcctgct ggggtagtcc gcgtggctgg ccctttcgca agtcaccaag ccgtccgtct cgttgttcgt cgtcgacgcc acgacgacgt cgccgccttt agctcaatcg acgaatcaac ccccggtagc cctacccccg acctttccca agtaccccag ccggcgacac tcgcttctcc ggctcttcga catcttacga ttgggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat