CrneC.00459.a
Choline kinase
| CENTER ID: | CrneC.00459.a |
| ORGANISM: | Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
| ASSOCIATED DISEASE: | Pneumonia-like illness |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.00459.a.B2.GE41248 | Truncation 1 | 88 | 519 | ||
| CrneC.00459.a.B3.GE41249 | Truncation 2 | 188 | 519 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.00459.a.B2.PW38290 | Truncation 1 | 88 | 519 |
Structures
6WHP
DEPOSITED: 4/8/2020
DETERMINATION: XRay
CLONE: CrneC.00459.a.B2.GE41248
PROTEIN: CrneC.00459.a.B2.PW38290
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CNAG_04408 |
| UniProt: | J9VWY8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVVTPTRPPV EPSGPPLSPL ALASAAHANS SDSYFSAQAG SRPLPRHRRT SGSSFSKLSE FSLDPPLLDK TDQYAASPDQ IDENGVTGVR HVALSVDASE WRQPVFKQKV LAILRRLHVP RWSSPLLTPT NIHLQKVSGA LTNAVFFVSF NPAPNPTSPS ESPLLTPTIP PSDPSHPPPL TPEQYPHTLL FRVYGPSSDA LISRSEELRI LHVLSTQYGI GPRVFGTFTN GRVEEFFPSR ALTAQELRDP IISRGIARRM RELHSVDLRR LGYEQGRATE PALWICLKEW SEAAEDVISS LTALGGTLEA WVERFSLHRI REEVTIYRNF VESQSGKGNG VVFAHNDTQY GNLLRLDVEL PPNTPEHCRY IVIDFEYASP NPRGYDIANH FHEWRANYHH PTHSHSLIPH FPYPTPIQRE DFYRSYLSVE VDGRNGEEVV GKRKDVPADK VAALEHEVRI WSPGCSINWA LWGLVQAEEQ VCALATKKEG YVPEFDYLSY AAERLEMFRD EAKKLGVPL
NT Sequence
atggtggtca cccccacccg tccgcccgtc gagccatctg gccctcccct ctcccccttg gcgctcgcct ccgctgcaca tgcaaacagc tcggactcct acttctccgc ccaagccgga tcgaggccgc tacccaggca tagaaggaca tcaggaagca gtttctcaaa gttatccgag ttcagcttgg atcctcctct tctcgacaaa acggatcagt acgctgcctc gcctgaccag atcgatgaaa acggcgtcac cggtgttcgg catgttgcgt tgagtgtgga tgccagcgaa tggcggcagc cagttttcaa acaaaaagtc ctagccattt tacgacgcct tcacgtcccg agatggtcct ctcctctttt aacacccacc aacatccatc ttcagaaagt ctcgggggcc ttgacaaatg ccgtcttctt tgtctctttc aatcccgctc ctaacccaac ctcgccttcc gaatcgcccc tgctaacccc aaccattcct ccatccgatc catcccatcc gccgccgctt actcccgaac aatatcccca cacccttctt ttcagagtct atggcccttc ctctgatgca ctcatctccc gatcggaaga attacgtata ctccacgtcc ttagcactca atatggcatt ggccccagag tatttggcac ctttaccaat ggtcgagtcg aggaattctt cccatcacgc gctttgaccg cgcaagaatt acgcgaccct attatttctc gtggtatcgc ccgacggatg cgcgaacttc actcggtgga cttgcgtcgc ttgggatacg aacaaggtcg tgccacggag cccgctttat ggatatgtct caaagaatgg tctgaagcag cggaagatgt catcagctca ttaacggctc tcggcgggac actggaagca tgggtggagc gtttctcttt acatcgtatc cgggaagaag tcacaatcta tcggaacttt gtagaatcac aaagcggaaa gggcaatggt gttgtgtttg ctcacaacga cacgcagtat ggaaatttgt tacgcctcga tgttgaactt ccgcccaaca ctcctgaaca ttgtcgttat attgtgattg attttgagta tgcttctccc aacccccgtg ggtacgatat cgccaaccat ttccacgaat ggcgagccaa ctaccaccat ccaactcact ctcactccct cattcctcat ttcccctacc ccacacctat tcagcgtgaa gacttttata gatcatactt gtcagtcgaa gtagacggaa ggaacggcga agaagtggta ggtaaacgca aggatgtccc agcagacaag gttgctgccc ttgaacatga agtaaggatt tggagtccgg ggtgcagtat aaactgggcg ctgtggggtt tagttcaagc tgaagaacag gtctgtgcct tggccacgaa gaaggaaggg tatgttccag aattcgatta tctctcgtac gccgctgagc gacttgaaat gttccgggac gaagccaaga agcttggagt tccgttatag
Details for CrneC.00459.a.B2.GE41248
| HARVESTED ON: | 3/8/2017 |
| SEQUENCED ON: | 3/9/2017 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGV RHVALSVDAS EWRQPVFKQK VLAILRRLHV PRWSSPLLTP TNIHLQKVSG ALTNAVFFVS FNPAPNPTSP SESPLLTPTI PPSDPSHPPP LTPEQYPHTL LFRVYGPSSD ALISRSEELR ILHVLSTQYG IGPRVFGTFT NGRVEEFFPS RALTAQELRD PIISRGIARR MRELHSVDLR RLGYEQGRAT EPALWICLKE WSEAAEDVIS SLTALGGTLE AWVERFSLHR IREEVTIYRN FVESQSGKGN GVVFAHNDTQ YGNLLRLDVE LPPNTPEHCR YIVIDFEYAS PNPRGYDIAN HFHEWRANYH HPTHSHSLIP HFPYPTPIQR EDFYRSYLSV EVDGRNGEEV VGKRKDVPAD KVAALEHEVR IWSPGCSINW ALWGLVQAEE QVCALATKKE GYVPEFDYLS YAAERLEMFR DEAKKLGVPL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtgttc ggcatgttgc gttgagtgtg gatgccagcg aatggcggca gccagttttc aaacaaaaag tcctagccat tttacgacgc cttcacgtcc cgagatggtc ctctcctctt ttaacaccca ccaacatcca tcttcagaaa gtctcggggg ccttgacaaa tgccgtcttc tttgtctctt tcaatcccgc tcctaaccca acctcgcctt ccgaatcgcc cctgctaacc ccaaccattc ctccatccga tccatcccat ccgccgccgc ttactcccga acaatatccc cacacccttc ttttcagagt ctatggccct tcctctgatg cactcatctc ccgatcggaa gaattacgta tactccacgt ccttagcact caatatggca ttggccccag agtatttggc acctttacca atggtcgagt cgaggaattc ttcccatcac gcgctttgac cgcgcaagaa ttacgcgacc ctattatttc tcgtggtatc gcccgacgga tgcgcgaact tcactcggtg gacttgcgtc gcttgggata cgaacaaggt cgtgccacgg agcccgcttt atggatatgt ctcaaagaat ggtctgaagc agcggaagat gtcatcagct cattaacggc tctcggcggg acactggaag catgggtgga gcgtttctct ttacatcgta tccgggaaga agtcacaatc tatcggaact ttgtagaatc acaaagcgga aagggcaatg gtgttgtgtt tgctcacaac gacacgcagt atggaaattt gttacgcctc gatgttgaac ttccgcccaa cactcctgaa cattgtcgtt atattgtgat tgattttgag tatgcttctc ccaacccccg tgggtacgat atcgccaacc atttccacga atggcgagcc aactaccacc atccaactca ctctcactcc ctcattcctc atttccccta ccccacacct attcagcgtg aagactttta tagatcatac ttgtcagtcg aagtagacgg aaggaacggc gaagaagtgg taggtaaacg caaggatgtc ccagcagaca aggttgctgc ccttgaacat gaagtaagga tttggagtcc ggggtgcagt ataaactggg cgctgtgggg tttagttcaa gctgaagaac aggtctgtgc cttggccacg aagaaggaag ggtatgttcc agaattcgat tatctctcgt acgccgctga gcgacttgaa atgttccggg acgaagccaa gaagcttgga gttccgttat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for CrneC.00459.a.B3.GE41249
| HARVESTED ON: | 3/8/2017 |
| SEQUENCED ON: | 3/9/2017 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMT LLFRVYGPSS DALISRSEEL RILHVLSTQY GIGPRVFGTF TNGRVEEFFP SRALTAQELR DPIISRGIAR RMRELHSVDL RRLGYEQGRA TEPALWICLK EWSEAAEDVI SSLMALGGTL EAWVERFSLH RIREEVTIYR NFVESQSGKG NGVVFAHNDT QYGNLLRLDV ELPPNTPEHC RYIVIDFEYA SPNPRGYDIA NHFHEWRANY HHPTHSHSLI PHFPYPTPIQ REDFYRSYLS VEVDGRNGEE VVGKRKDVPA DKVAALEHEV RIWSPGCSIN WALWGLVQAE EQVCALATKK EGYVPEFDYL SYAAERLEMF RD
Validated NT Sequence
atggctcacc accaccacca ccatatgacc cttcttttca gagtctatgg cccttcctct gatgcactca tctcccgatc ggaagaatta cgtatactcc acgtccttag cactcaatat ggcattggcc ccagagtatt tggcaccttt accaatggtc gagtcgagga attcttccca tcacgcgctt tgaccgcgca agaattacgc gaccctatta tttctcgtgg tatcgcccga cggatgcgcg aacttcactc ggtggacttg cgtcgcttgg gatacgaaca aggtcgtgcc acggagcccg ctttatggat atgtctcaaa gaatggtctg aagcagcgga agatgtcatc agctcattaa tggctctcgg cgggacactg gaagcatggg tggagcgttt ctctttacat cgtatccggg aagaagtcac aatctatcgg aactttgtag aatcacaaag cggaaagggc aatggtgttg tgtttgctca caacgacacg cagtatggaa atttgttacg cctcgatgtt gaacttccgc ccaacactcc tgaacattgt cgttatattg tgattgattt tgagtatgct tctcccaacc cccgtgggta cgatatcgcc aaccatttcc acgaatggcg agccaactac caccatccaa ctcactctca ctccctcatt cctcatttcc cctaccccac acctattcag cgtgaagact tttatagatc atacttgtca gtcgaagtag acggaaggaa cggcgaagaa gtggtaggta aacgcaagga tgtcccagca gacaaggttg ctgcccttga acatgaagta aggatttgga gtccggggtg cagtataaac tgggcgctgt ggggtttagt tcaagctgaa gaacaggtct gtgccttggc cacgaagaag gaagggtatg ttccagaatt cgattatctc tcgtacgccg ctgagcgact tgaaatgttc cgggacga
Expected Protein Sequence
MAHHHHHHTL LFRVYGPSSD ALISRSEELR ILHVLSTQYG IGPRVFGTFT NGRVEEFFPS RALTAQELRD PIISRGIARR MRELHSVDLR RLGYEQGRAT EPALWICLKE WSEAAEDVIS SLTALGGTLE AWVERFSLHR IREEVTIYRN FVESQSGKGN GVVFAHNDTQ YGNLLRLDVE LPPNTPEHCR YIVIDFEYAS PNPRGYDIAN HFHEWRANYH HPTHSHSLIP HFPYPTPIQR EDFYRSYLSV EVDGRNGEEV VGKRKDVPAD KVAALEHEVR IWSPGCSINW ALWGLVQAEE QVCALATKKE GYVPEFDYLS YAAERLEMFR DEAKKLGVPL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacccttc ttttcagagt ctatggccct tcctctgatg cactcatctc ccgatcggaa gaattacgta tactccacgt ccttagcact caatatggca ttggccccag agtatttggc acctttacca atggtcgagt cgaggaattc ttcccatcac gcgctttgac cgcgcaagaa ttacgcgacc ctattatttc tcgtggtatc gcccgacgga tgcgcgaact tcactcggtg gacttgcgtc gcttgggata cgaacaaggt cgtgccacgg agcccgcttt atggatatgt ctcaaagaat ggtctgaagc agcggaagat gtcatcagct cattaacggc tctcggcggg acactggaag catgggtgga gcgtttctct ttacatcgta tccgggaaga agtcacaatc tatcggaact ttgtagaatc acaaagcgga aagggcaatg gtgttgtgtt tgctcacaac gacacgcagt atggaaattt gttacgcctc gatgttgaac ttccgcccaa cactcctgaa cattgtcgtt atattgtgat tgattttgag tatgcttctc ccaacccccg tgggtacgat atcgccaacc atttccacga atggcgagcc aactaccacc atccaactca ctctcactcc ctcattcctc atttccccta ccccacacct attcagcgtg aagactttta tagatcatac ttgtcagtcg aagtagacgg aaggaacggc gaagaagtgg taggtaaacg caaggatgtc ccagcagaca aggttgctgc ccttgaacat gaagtaagga tttggagtcc ggggtgcagt ataaactggg cgctgtgggg tttagttcaa gctgaagaac aggtctgtgc cttggccacg aagaaggaag ggtatgttcc agaattcgat tatctctcgt acgccgctga gcgacttgaa atgttccggg acgaagccaa gaagcttgga gttccgttat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for CrneC.00459.a.B2.PW38290
| PURIFICATION DATe: | 5/31/2017 |
| CONCENTRATION: | 32.4mg/ml |
| OBSERVED MW: | 59kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHGV RHVALSVDAS EWRQPVFKQK VLAILRRLHV PRWSSPLLTP TNIHLQKVSG ALTNAVFFVS FNPAPNPTSP SESPLLTPTI PPSDPSHPPP LTPEQYPHTL LFRVYGPSSD ALISRSEELR ILHVLSTQYG IGPRVFGTFT NGRVEEFFPS RALTAQELRD PIISRGIARR MRELHSVDLR RLGYEQGRAT EPALWICLKE WSEAAEDVIS SLTALGGTLE AWVERFSLHR IREEVTIYRN FVESQSGKGN GVVFAHNDTQ YGNLLRLDVE LPPNTPEHCR YIVIDFEYAS PNPRGYDIAN HFHEWRANYH HPTHSHSLIP HFPYPTPIQR EDFYRSYLSV EVDGRNGEEV VGKRKDVPAD KVAALEHEVR IWSPGCSINW ALWGLVQAEE QVCALATKKE GYVPEFDYLS YAAERLEMFR DEAKKLGVPL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGTGTTC GGCATGTTGC GTTGAGTGTG GATGCCAGCG AATGGCGGCA GCCAGTTTTC AAACAAAAAG TCCTAGCCAT TTTACGACGC CTTCACGTCC CGAGATGGTC CTCTCCTCTT TTAACACCCA CCAACATCCA TCTTCAGAAA GTCTCGGGGG CCTTGACAAA TGCCGTCTTC TTTGTCTCTT TCAATCCCGC TCCTAACCCA ACCTCGCCTT CCGAATCGCC CCTGCTAACC CCAACCATTC CTCCATCCGA TCCATCCCAT CCGCCGCCGC TTACTCCCGA ACAATATCCC CACACCCTTC TTTTCAGAGT CTATGGCCCT TCCTCTGATG CACTCATCTC CCGATCGGAA GAATTACGTA TACTCCACGT CCTTAGCACT CAATATGGCA TTGGCCCCAG AGTATTTGGC ACCTTTACCA ATGGTCGAGT CGAGGAATTC TTCCCATCAC GCGCTTTGAC CGCGCAAGAA TTACGCGACC CTATTATTTC TCGTGGTATC GCCCGACGGA TGCGCGAACT TCACTCGGTG GACTTGCGTC GCTTGGGATA CGAACAAGGT CGTGCCACGG AGCCCGCTTT ATGGATATGT CTCAAAGAAT GGTCTGAAGC AGCGGAAGAT GTCATCAGCT CATTAACGGC TCTCGGCGGG ACACTGGAAG CATGGGTGGA GCGTTTCTCT TTACATCGTA TCCGGGAAGA AGTCACAATC TATCGGAACT TTGTAGAATC ACAAAGCGGA AAGGGCAATG GTGTTGTGTT TGCTCACAAC GACACGCAGT ATGGAAATTT GTTACGCCTC GATGTTGAAC TTCCGCCCAA CACTCCTGAA CATTGTCGTT ATATTGTGAT TGATTTTGAG TATGCTTCTC CCAACCCCCG TGGGTACGAT ATCGCCAACC ATTTCCACGA ATGGCGAGCC AACTACCACC ATCCAACTCA CTCTCACTCC CTCATTCCTC ATTTCCCCTA CCCCACACCT ATTCAGCGTG AAGACTTTTA TAGATCATAC TTGTCAGTCG AAGTAGACGG AAGGAACGGC GAAGAAGTGG TAGGTAAACG CAAGGATGTC CCAGCAGACA AGGTTGCTGC CCTTGAACAT GAAGTAAGGA TTTGGAGTCC GGGGTGCAGT ATAAACTGGG CGCTGTGGGG TTTAGTTCAA GCTGAAGAAC AGGTCTGTGC CTTGGCCACG AAGAAGGAAG GGTATGTTCC AGAATTCGAT TATCTCTCGT ACGCCGCTGA GCGACTTGAA ATGTTCCGGG ACGAAGCCAA GAAGCTTGGA GTTCCGTTAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT