CrneC.00439.b

CDC7 protein kinase

CENTER ID: CrneC.00439.b
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.00439.b.B3.GE41391 kinase domain truncation 2 265 671
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00636
UniProt: J9VJH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEEEPTLDPF TSQPPLTRAA AKRLATASPS KPSPARSAKR VREATPIVAD DGYSTPRATT VTPMKGMKGL REPADEQGEA CPNAHLTMGS IDVNTSEAES VLFSEKTTVL QNEEVEGTLE TDGSASQSLS VMTGDIGRHT YRDDFTRGEQ DLFMPEATST STHRYTTKDR ITMPPPTDVP SHKLRSSSLY DPFTAPAAEG TKLDRMTEVL EKQKKRSFTE MDVSEFPLEE EWDEEGEAGD PYSDESYHSG DTEDYEMDHR PVLQRTAIKK DIRDFTNSLS LLRDGSIHGI PYKVVDRLGE GTFSSVYLAY DSLHHLHSNE YWLGQNDGAN QIPDKHAVRV ALKKILVTSS AIRIENELAI LEDLRGCRNV SQLITAFREE DQVIIVLPYH QCDDFRHFFK HMDPHHMRSY MRDLFRSLKD IHQRGIIHRD VKPANFLYDY ESETGVLVDF GLAERYCPPQ EATCQHAPAT STSLQGFKIK TPDTSAVEQA VYDARKRSKL GEGRVGFPHE DKRPAIRTNR AGTRGFRAPE VLLKCPDQTV AVDVWSAGII LFSILTQKFP AFNSSDDIEA LMEIAAIFGK TAMERCALLH NRTMISNVPT LDDPPSSLTE LILTLNPHLY TPHSSSPTPE EAKEHIEAVD DALDLVTKLL RLDCTKRLTA AQALRHPFVT GRDGEWDEYD DEEVLSSKRE AECW
NT Sequence
atggaagagg agcccactct tgaccctttt acttcgcaac cgccattgac tcgtgcagcg gccaagcgtc tcgcgaccgc ttctcccagc aaaccttctc ccgcccgttc agccaaacgc gtcagagaag cgactccaat tgttgctgat gacgggtatt caactcctcg agcaaccacc gtgactccta tgaagggtat gaaagggctg agagagccag cagatgaaca gggcgaggca tgtcctaatg cgcatcttac tatggggtcc attgacgtta acacgagtga agcagaaagc gtgcttttct cggaaaagac cacagttcta caaaacgagg aagtggaagg aaccctggag acagacggat ctgcaagtca aagtctgtct gtgatgacgg gcgacatcgg tcgacacacg tatagggatg acttcactcg aggagaacaa gacctgttca tgcccgaagc gacgagtaca tcaacccatc gatatacgac caaggatcgt atcaccatgc cccctcctac cgatgttcca tcccataaac tccgctcatc atctctatac gaccctttca ccgctccagc tgctgagggc actaagctcg atcgcatgac cgaagtcctt gagaagcaga agaagcgttc tttcacagaa atggatgtct ctgaatttcc tttggaagag gagtgggatg aagagggcga agcgggagat ccgtattcag acgaaagcta ccattccggg gacacggagg actatgagat ggatcacagg cctgtactgc agagaacagc tatcaagaag gatatcaggg attttaccaa cagtttgagc ctgctaagag atggaagcat tcatgggatt ccatataaag tggttgaccg actaggggaa ggaacgttct cttcggtata tctcgcctat gactccttgc atcaccttca ttcaaatgag tactggctcg gccagaacga tggggccaat caaattcccg ataagcacgc agtccgagtt gcgttgaaga agattttggt cacaagcagc gcaatcagaa tagaaaatga attggctatc ttagaagatt tgaggggctg tcgcaatgtc tcgcaactaa tcactgcgtt tcgtgaggaa gatcaggtca ttattgtcct tccatatcat cagtgcgacg attttaggca ttttttcaaa cacatggatc cacaccacat gaggtcatac atgcgtgacc tcttccggtc acttaaggac atccaccaga gaggtattat tcatcgtgat gtgaaacctg caaacttctt gtatgattat gagagcgaaa ctggcgtcct ggtagatttt ggtcttgctg aacgctattg cccaccacag gaagccacat gccagcacgc acccgccaca tccacttctc tacaaggctt caagatcaaa acacccgata cttctgcggt tgaacaagct gtatatgatg cgcgcaagcg ctccaaatta ggtgaaggaa gagttgggtt cccccacgag gacaaaagac ccgcgatcag gacaaatagg gctggaacaa gaggttttag agcgcctgaa gtgttgctga agtgcccgga tcagactgtc gcggtcgacg tttggtcggc tggtatcata cttttttcta tactgacgca aaaattcccg gcattcaatt caagcgacga tattgaagct ttgatggaga ttgctgcaat attcggaaaa acagccatgg aacggtgtgc cttactgcat aatcgtacta tgatatcaaa tgtgccaaca ctcgacgacc ctccttcttc tttgaccgaa ctaatcctta cgctcaatcc gcatctttac acccctcata gctcaagtcc tacgccagaa gaagcgaagg aacacatcga agccgttgat gatgctcttg atttggtcac aaagcttctt cgattggact gtaccaagcg gcttactgct gcacaagctc tgagacaccc atttgtgact gggagggatg gtgaatggga tgagtatgac gatgaggaag ttttgtcgag taagcgtgaa gcggaatgtt ggtga
Details for CrneC.00439.b.B3.GE41391
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR TAIKKDIRDF TNSLSLLRDG SIHGIPYKVV DRLGEGTFSS VYLAYDSLHH LHSNEYWLGQ NDGANQIPDK HAVRVALKKI LVTSSAIRIE NELAILEDLR GCRNVSQLIT AFREEDQVII VLPYHQCDDF RHFFKHMDPH HMRSYMRDLF RSLKDIHQRG IIHRDVKPAN FLYDYESETG VLVDFGLAER YSPPQEATCQ HAPATSTSLQ GFKIKTPDTS AVEQAVYDAR KRSKLGEGRV GFPHEDKRPA IRTNRAGTRG FRAPEVLLKC PDQTVAVDVW SAGIILFSIL TQKFPAFNSS DDIEALMEIA AIFGKTAMER CALLHNRTMI SNVPTLDDPP SSLTELILTL NPHLYTPHSS SPTPEEAKEH IEAVDDALDL VTKLLRLDCT KRLTAAQA
Validated NT Sequence
atggctcacc accaccacca ccatatgaga acagctatca agaaggatat cagggatttt accaacagtt tgagcctgct aagagatgga agcattcatg ggattccata taaagtggtt gaccgactag gggaaggaac gttctcttcg gtatatctcg cctatgactc cttgcatcac cttcattcaa atgagtactg gctcggccag aacgatgggg ccaatcaaat tcccgataag cacgcagtcc gagttgcgtt gaagaagatt ttggtcacaa gcagcgcaat cagaatagaa aatgaattgg ctatcttaga agatttgagg ggctgtcgca atgtctcgca actaatcact gcgtttcgtg aggaagatca ggtcattatt gtccttccat atcatcagtg cgacgatttt aggcattttt tcaaacacat ggatccacac cacatgaggt catacatgcg tgacctcttc cggtcactta aggacatcca ccagagaggt attattcatc gtgatgtgaa acctgcaaac ttcttgtatg attatgagag cgaaactggc gtcctggtag attttggtct tgctgaacgc tattccccac cacaggaagc cacatgccag cacgcacccg ccacatccac ttctctacaa ggcttcaaga tcaaaacacc cgatacttct gcggttgaac aagctgtata tgatgcgcgc aagcgctcca aattaggtga aggaagagtt gggttccccc acgaggacaa aagacccgcg atcaggacaa atagggctgg aacaagaggt tttagagcgc ctgaagtgtt gctgaagtgc ccggatcaga ctgtcgcggt cgacgtttgg tcggctggta tcatactttt ttctatactg acgcaaaaat tcccggcatt caattcaagc gacgatattg aagctttgat ggagattgct gcaatattcg gaaaaacagc catggaacgg tgtgccttac tgcataatcg tactatgata tcaaatgtgc caacactcga cgaccctcct tcttctttga ccgaactaat ccttacgctc aatccgcatc tttacacccc tcatagctca agtcctacgc cagaagaagc gaaggaacac atcgaagccg ttgatgatgc tcttgatttg gtcacaaagc ttcttcgatt ggactgtacc aagcggctta ctgctgcaca agctc
Expected Protein Sequence
MAHHHHHHRT AIKKDIRDFT NSLSLLRDGS IHGIPYKVVD RLGEGTFSSV YLAYDSLHHL HSNEYWLGQN DGANQIPDKH AVRVALKKIL VTSSAIRIEN ELAILEDLRG CRNVSQLITA FREEDQVIIV LPYHQCDDFR HFFKHMDPHH MRSYMRDLFR SLKDIHQRGI IHRDVKPANF LYDYESETGV LVDFGLAERY CPPQEATCQH APATSTSLQG FKIKTPDTSA VEQAVYDARK RSKLGEGRVG FPHEDKRPAI RTNRAGTRGF RAPEVLLKCP DQTVAVDVWS AGIILFSILT QKFPAFNSSD DIEALMEIAA IFGKTAMERC ALLHNRTMIS NVPTLDDPPS SLTELILTLN PHLYTPHSSS PTPEEAKEHI EAVDDALDLV TKLLRLDCTK RLTAAQALRH PFVTG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagaacag ctatcaagaa ggatatcagg gattttacca acagtttgag cctgctaaga gatggaagca ttcatgggat tccatataaa gtggttgacc gactagggga aggaacgttc tcttcggtat atctcgccta tgactccttg catcaccttc attcaaatga gtactggctc ggccagaacg atggggccaa tcaaattccc gataagcacg cagtccgagt tgcgttgaag aagattttgg tcacaagcag cgcaatcaga atagaaaatg aattggctat cttagaagat ttgaggggct gtcgcaatgt ctcgcaacta atcactgcgt ttcgtgagga agatcaggtc attattgtcc ttccatatca tcagtgcgac gattttaggc attttttcaa acacatggat ccacaccaca tgaggtcata catgcgtgac ctcttccggt cacttaagga catccaccag agaggtatta ttcatcgtga tgtgaaacct gcaaacttct tgtatgatta tgagagcgaa actggcgtcc tggtagattt tggtcttgct gaacgctatt gcccaccaca ggaagccaca tgccagcacg cacccgccac atccacttct ctacaaggct tcaagatcaa aacacccgat acttctgcgg ttgaacaagc tgtatatgat gcgcgcaagc gctccaaatt aggtgaagga agagttgggt tcccccacga ggacaaaaga cccgcgatca ggacaaatag ggctggaaca agaggtttta gagcgcctga agtgttgctg aagtgcccgg atcagactgt cgcggtcgac gtttggtcgg ctggtatcat acttttttct atactgacgc aaaaattccc ggcattcaat tcaagcgacg atattgaagc tttgatggag attgctgcaa tattcggaaa aacagccatg gaacggtgtg ccttactgca taatcgtact atgatatcaa atgtgccaac actcgacgac cctccttctt ctttgaccga actaatcctt acgctcaatc cgcatcttta cacccctcat agctcaagtc ctacgccaga agaagcgaag gaacacatcg aagccgttga tgatgctctt gatttggtca caaagcttct tcgattggac tgtaccaagc ggcttactgc tgcacaagct ctgagacacc catttgtgac tgggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat