SSGCID: Target Page

CrneC.00439.a

CMGC/CK2 protein kinase

CENTER ID:	CrneC.00439.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.00439.a.B1.GE41247	full length		1	338

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_05694
UniProt:	J9VNH4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGGRSVARV YANVNEKLGR SWWDYDNLVV QWGVQDNYEI VRKVGRGKYS EVFESIHLPT DSKCIVKVLK PVKKKKIKRE IKILQNLAGG PNVVGLLDVV RDSQSKTPSI VTEYVNNTEF KTLYPKFSDF DVRYYIFELL KALDFCHSKG IMHRDVKPHN VMIDHEKRTL RLIDWGLAEF YHPGTEYNVR VASRYFKGPE LLVDFQEYDY SLDMWSLGCM FASMIFRKEP FFHGHDNADQ LVKIAKVLGT DELYTYLERY DIDLDAQFDD ILGRYPRKPW SRFVSSENQR YISSEAIDFL DKLLRYDHQE RLTAEEAKEH PYFEPVRQAA AQASASQP

NT Sequence

atgtcaggtg gacgtagtgt ggctcgtgtc tatgccaacg tgaacgagaa gcttggaaga tcctggtggg actatgataa tcttgtggtt caatggggag ttcaagataa ctatgagatt gtaaggaagg ttgggcgagg aaagtactcc gaagtttttg aatccattca tctcccaacc gactccaaat gtattgtcaa ggtcttaaag cctgttaaga agaagaagat caagcgagaa ataaaaatat tgcagaattt ggctggtgga cccaatgtgg tgggactgtt ggacgttgtt agagacagtc agtcaaagac accctccatt gttacggaat atgtgaacaa caccgaattc aagacgcttt atcccaaatt ttcagatttc gatgtgcgat actacatctt tgagcttctc aaagcgttgg atttctgtca ttccaagggc atcatgcacc gcgacgttaa gccgcacaac gtcatgattg atcacgagaa gcggacgtta cggctgattg attggggact tgctgaattt taccatcctg gcacggaata caacgtgcgt gtggcttcaa gatatttcaa aggaccagaa ctgttggtcg actttcaaga atatgattac agtttggata tgtggagtct agggtgcatg ttcgctagca tgattttccg aaaagagccg ttctttcacg gtcatgacaa cgctgatcag ctggtcaaaa ttgctaaagt cctcggcaca gatgaactct acacttatct tgagcgatat gatattgatc ttgatgctca gtttgacgat atattagggc gatatcctcg aaagccctgg tcccgattcg tctcatccga aaaccagcgt tatatctcca gcgaggctat agattttctg gataagcttt taaggtacga ccaccaggaa agattaaccg cggaggaggc caaggagcac ccgtactttg agcccgtgcg acaagcggcc gctcaagctt ccgcctctca accatga

Details for CrneC.00439.a.B1.GE41247

HARVESTED ON:	3/8/2017
SEQUENCED ON:	3/9/2017
EXPECTED MW:	41kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS GGRSVARVYA NVNEKLGRSW WDYDNLVVQW GVQDNYEIVR KVGRGKYSEV FESIHLPTDS KCIVKVLKPV KKKKIKREIK ILQNLAGGPN VVGLLDVVRD SQSKTPSIVT EYVNNTEFKT LYPKFSDFDV RYYIFELLKA LDFCHSKGIM HRDVKPHNVM IDHEKRTLRL IDWGLAEFYH PGTEYNVRVA SRYFKGPELL VDFQEYDYSL DMWSLGCMFA SMIFRKEPFF HGHDNADQLV KIAKVLGTDE LYTYLERYDI DLDAQFDDIL GRYPRKPWSR FVSSENQRYI SSEAIDFLDK LLRYDHQERL TAEEAKEHPY FEPVRQA

Validated NT Sequence

atggctcacc accaccacca ccatatgtca ggtggacgta gtgtggctcg tgtctatgcc aacgtgaacg agaagcttgg aagatcctgg tgggactatg ataatcttgt ggttcaatgg ggagttcaag ataactatga gattgtaagg aaggttgggc gaggaaagta ctccgaagtt tttgaatcca ttcatctccc aaccgactcc aaatgtattg tcaaggtctt aaagcctgtt aagaagaaga agatcaagcg agaaataaaa atattgcaga atttggctgg tggacccaat gtggtgggac tgttggacgt tgttagagac agtcagtcaa agacaccctc cattgttacg gaatatgtga acaacaccga attcaagacg ctttatccca aattttcaga tttcgatgtg cgatactaca tctttgagct tctcaaagcg ttggatttct gtcattccaa gggcatcatg caccgcgacg ttaagccgca caacgtcatg attgatcacg agaagcggac gttacggctg attgattggg gacttgctga attttaccat cctggcacgg aatacaacgt gcgtgtggct tcaagatatt tcaaaggacc agaactgttg gtcgactttc aagaatatga ttacagtttg gatatgtgga gtctagggtg catgttcgct agcatgattt tccgaaaaga gccgttcttt cacggtcatg acaacgctga tcagctggtc aaaattgcta aagtcctcgg cacagatgaa ctctacactt atcttgagcg atatgatatt gatcttgatg ctcagtttga cgatatatta gggcgatatc ctcgaaagcc ctggtcccga ttcgtctcat ccgaaaacca gcgttatatc tccagcgagg ctatagattt tctggataag cttttaaggt acgaccacca ggaaagatta accgcggagg aggccaagga gcacccgtac tttgagcccg tgcgacaagc ggcc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag gtggacgtag tgtggctcgt gtctatgcca acgtgaacga gaagcttgga agatcctggt gggactatga taatcttgtg gttcaatggg gagttcaaga taactatgag attgtaagga aggttgggcg aggaaagtac tccgaagttt ttgaatccat tcatctccca accgactcca aatgtattgt caaggtctta aagcctgtta agaagaagaa gatcaagcga gaaataaaaa tattgcagaa tttggctggt ggacccaatg tggtgggact gttggacgtt gttagagaca gtcagtcaaa gacaccctcc attgttacgg aatatgtgaa caacaccgaa ttcaagacgc tttatcccaa attttcagat ttcgatgtgc gatactacat ctttgagctt ctcaaagcgt tggatttctg tcattccaag ggcatcatgc accgcgacgt taagccgcac aacgtcatga ttgatcacga gaagcggacg ttacggctga ttgattgggg acttgctgaa ttttaccatc ctggcacgga atacaacgtg cgtgtggctt caagatattt caaaggacca gaactgttgg tcgactttca agaatatgat tacagtttgg atatgtggag tctagggtgc atgttcgcta gcatgatttt ccgaaaagag ccgttctttc acggtcatga caacgctgat cagctggtca aaattgctaa agtcctcggc acagatgaac tctacactta tcttgagcga tatgatattg atcttgatgc tcagtttgac gatatattag ggcgatatcc tcgaaagccc tggtcccgat tcgtctcatc cgaaaaccag cgttatatct ccagcgaggc tatagatttt ctggataagc ttttaaggta cgaccaccag gaaagattaa ccgcggagga ggccaaggag cacccgtact ttgagcccgt gcgacaagcg gccgctcaag cttccgcctc tcaaccatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at