CrneC.00433.a

IKS protein kinase

CENTER ID: CrneC.00433.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.00433.a.B21.GE41808 Truncation 1 132 513
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06809
UniProt: J9VSH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMDIEDPSRD MAGAGELEQD WRPIGHTSNQ LVLYHPASHA LRVRIQPPSS PRSTFPSNIV RANDALKRRS PDLATRPLCL LCRQPVSRNG LGEVAAISGM DSYFQTLEHV HSESESFSEG MGIDNLLNAP QMDTSNEENS SATGYYVRFF KEVRKLGMGA EGSVYLAVHH IEGDTLGTYA VKKIAVGESK EYLHKILHEV KLLEVLRHTN IIPYYHAWID TTRFSDLAPP VLALHVLMMY ASGGNLDSLL LSRSHSTGFA TGLSADDIAD AESIDKLPKE ERIKAFKRRR QSAKTEWANN RRSFSGGMGA SRAENRGVLL LSFEEIVQLF GDIIEGLSFL HSNSILHLDL KCSNVLLHWE EGKLVPKAML SDFGTSEEML RGQRERTGHT GTMEYMAPET LVQDSEGRFR PSDSHADMWS LGMILYKMMF LKLPYEECDN YDVLRAQIIS YKGFLPDGSL LQNLQRRHIP QRFVFLLKKL LDIHPEKRPS AEKTRSVLAD IKHHSNQNED YSSAIVGQTL ASLENLSYPN RGICRRNSVV VNDGHLNADA TASNNVLLPF FPSPKKKLLP SFIDGHERAM TCLAGPAVRL ALLFGKLVSI QPSISGVRLP PAVAVILILL ALTDLAHQGS LLWSLTCLIA HCAAVLNFSS HEV
NT Sequence
atgatggata tcgaggaccc cagcagggac atggcaggcg cgggagaatt ggaacaggac tggagaccca ttggccatac ttcaaatcag ctcgttttat atcatcctgc ctcacatgct ctgcgagttc gcatacagcc gccctcgtca ccacgttcca ctttccctag caatatcgtg cgcgcgaacg atgcgcttaa gagacgatct cccgatttgg cgactagacc cctgtgcttg ctctgccgac aacccgtttc cagaaatggc cttggtgagg ttgctgcaat atcaggcatg gacagttatt tccaaacatt agaacatgtc cattcagaat ctgaaagttt ctcggaaggt atgggaattg acaatctgct gaacgccccg caaatggata cgtccaatga agagaattct tcagcaacag gctattatgt ccggtttttc aaagaagtac ggaaactagg aatgggcgct gagggaagtg tatatctggc tgtacatcac attgaggggg ataccttagg aacgtatgcg gtaaagaaaa tagctgtcgg ggaatccaag gaatatcttc acaaaatcct gcatgaagtg aagctgttgg aagttctgag gcataccaac attatacctt actaccatgc gtggattgac acgactcgat tctctgatct cgctcccccg gtgcttgctc tacatgttct catgatgtac gcttctggcg gaaatcttga ttcactactt ctttcacgat ctcattcgac aggattcgcg accggtctct cggcagacga cattgccgac gccgaatcaa tcgacaagtt gcccaaagaa gaacgcatta aggcgttcaa acgcagaagg caaagcgcca aaaccgagtg ggcaaacaat cggcgatcgt tttcaggtgg tatgggggcc tccagggcag aaaacagagg tgtactgcta cttagttttg aagaaatcgt ccagcttttc ggagacataa ttgagggact gtcttttctt cattcaaact ccattttgca tctggattta aaatgctcta acgttcttct gcactgggag gagggaaagt tggtaccgaa ggcgatgttg tccgattttg gcacttcaga agagatgctt agaggtcaga gagaaaggac aggccatact ggtaccatgg agtacatggc gccggagaca ctagtccaag acagtgaagg ccgttttcgg ccctctgatt cacatgcaga catgtggtct cttgggatga ttttgtacaa gatgatgttt ttgaaactgc catatgaaga atgcgacaac tatgatgttc ttcgtgccca aatcatttcc tataagggct tcctaccgga tgggtctcta ctgcagaatt tacagcgccg acatattcca caacgctttg tcttccttct caagaaatta ttggatatcc atccggaaaa gcgtccttct gctgagaaga cgagaagcgt tttagcggac attaaacatc actcaaatca aaatgaggat tattcttcgg cgatagttgg gcaaacgctt gcgtcattgg aaaacctctc ttacccaaac agaggtattt gtcgaagaaa ttcggtagtc gtcaacgacg gtcacctcaa tgccgacgca accgcgtcaa acaacgttct tcttcccttc ttcccgtcac caaaaaaaaa attgctacca tcattcatcg atggccatga gcgagcaatg acctgccttg ctggtccagc ggtgcgccta gcattgttgt tcggcaagct cgtttccatt cagcctagta tctccggtgt cagattaccg cctgctgttg cagtcatcct tattctctta gccttaactg atttagccca ccaaggatca cttctctggt ctttgacctg tttgatagct cattgtgctg cagttctcaa tttctcttcc cacgaggtat aa
Details for CrneC.00433.a.B21.GE41808
HARVESTED ON: 8/7/2017
SEQUENCED ON: 9/20/2017
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHMDT SNEENSSATG YYVRFFKEVR KLGMGAEGSV YLAVHHIEGD TLGTYAVKKI AVGESKEYLH KILHEVKLLE VLRHTNIIPY YHAWIDTTRF SDLAPPVLAL HVLMMYASGG NLDSLLLSRS HSTGFATGLS ADDIADAESI DKLPKEERIK AFKRRRQSAK TEWANNRRSF SGGMGASRAE NRGVLLLSFE EIVQLFGDII EGLSFLHSNS ILHLDLKCSN VLLHWEEGKL VPKAMLSDFG TSEEMLRGQR ERTGHTGTME YMAPETLVQD SEGRFRPSDS HADMWSLGMI LYKMMFLKLP YEECDNYDVL RAQIISYKGF LPDGSLLQNL QRRHIPQRFV FLLKKLLDIH PEKRPSAEKT RSVLADIKHH S
Validated NT Sequence
tttgagtgat gtttaatgtc cgctaaaacg cttctcgtct tctcagcaga aggacgcttt tccggatgga tatccaataa tttcttgaga aggaagacaa agcgttgtgg aatatgtcgg cgctgtaaat tctgcagtag agacccatcc ggtaggaagc ccttatagga aatgatttgg gcacgaagaa catcatagtt gtcgcattct tcatatggca gtttcaaaaa catcatcttg tacaaaatca tcccaagaga ccacatgtct gcatgtgaat cagagggccg aaaacggcct tcactgtctt ggactagtgt ctccggcgcc atgtactcca tggtaccagt atggcctgtc ctttctctct gacctctaag catctcttct gaagtgccaa aatcggacaa catcgccttc ggtaccaact ttccctcctc ccagtgcaga agaacgttag agcattttaa atccagatgc aaaatggagt ttgaatgaag aaaagacagt ccctcaatta tgtctccgaa aagctggacg atttcttcaa aactaagtag cagtacacct ctgttttctg ccctggaggc ccccatacca cctgaaaacg atcgccgatt gtttgcccac tcggttttgg cgctttgcct tctgcgtttg aacgccttaa tgcgttcttc tttgggcaac ttgtcgattg attcggcgtc ggcaatgtcg tctgccgaga gaccggtcgc gaatcctgtc gaatgagatc gtgaaagaag tagtgaatca agatttccgc cagaagcgta catcatgaga acatgtagag caagcaccgg gggagcgaga tcagagaatc gagtcgtgtc aatccacgca tggtagtaag gtataatgtt ggtatgcctc agaacttcca acagcttcac ttcatgcagg attttgtgaa gatattcctt ggattccccg acagctattt tctttaccgc atacgttcct aaggtatccc cctcaatgtg atgtacagcc agatatacac ttccctcagc gcccattcct agtttccgta cttctttgaa aaaccggaca taatagcctg ttgctgaaga attctcttca ttggacgtat ccatatggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMD TSNEENSSAT GYYVRFFKEV RKLGMGAEGS VYLAVHHIEG DTLGTYAVKK IAVGESKEYL HKILHEVKLL EVLRHTNIIP YYHAWIDTTR FSDLAPPVLA LHVLMMYASG GNLDSLLLSR SHSTGFATGL SADDIADAES IDKLPKEERI KAFKRRRQSA KTEWANNRRS FSGGMGASRA ENRGVLLLSF EEIVQLFGDI IEGLSFLHSN SILHLDLKCS NVLLHWEEGK LVPKAMLSDF GTSEEMLRGQ RERTGHTGTM EYMAPETLVQ DSEGRFRPSD SHADMWSLGM ILYKMMFLKL PYEECDNYDV LRAQIISYKG FLPDGSLLQN LQRRHIPQRF VFLLKKLLDI HPEKRPSAEK TRSVLADIKH HSNQNEDYSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata cgtccaatga agagaattct tcagcaacag gctattatgt ccggtttttc aaagaagtac ggaaactagg aatgggcgct gagggaagtg tatatctggc tgtacatcac attgaggggg ataccttagg aacgtatgcg gtaaagaaaa tagctgtcgg ggaatccaag gaatatcttc acaaaatcct gcatgaagtg aagctgttgg aagttctgag gcataccaac attatacctt actaccatgc gtggattgac acgactcgat tctctgatct cgctcccccg gtgcttgctc tacatgttct catgatgtac gcttctggcg gaaatcttga ttcactactt ctttcacgat ctcattcgac aggattcgcg accggtctct cggcagacga cattgccgac gccgaatcaa tcgacaagtt gcccaaagaa gaacgcatta aggcgttcaa acgcagaagg caaagcgcca aaaccgagtg ggcaaacaat cggcgatcgt tttcaggtgg tatgggggcc tccagggcag aaaacagagg tgtactgcta cttagttttg aagaaatcgt ccagcttttc ggagacataa ttgagggact gtcttttctt cattcaaact ccattttgca tctggattta aaatgctcta acgttcttct gcactgggag gagggaaagt tggtaccgaa ggcgatgttg tccgattttg gcacttcaga agagatgctt agaggtcaga gagaaaggac aggccatact ggtaccatgg agtacatggc gccggagaca ctagtccaag acagtgaagg ccgttttcgg ccctctgatt cacatgcaga catgtggtct cttgggatga ttttgtacaa gatgatgttt ttgaaactgc catatgaaga atgcgacaac tatgatgttc ttcgtgccca aatcatttcc tataagggct tcctaccgga tgggtctcta ctgcagaatt tacagcgccg acatattcca caacgctttg tcttccttct caagaaatta ttggatatcc atccggaaaa gcgtccttct gctgagaaga cgagaagcgt tttagcggac attaaacatc actcaaatca aaatgaggat tattcttcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat