CrneC.00424.c

CAMK protein kinase

CENTER ID: CrneC.00424.c
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.00424.c.B32.GE41806 Truncation 2 161 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_05216
UniProt: J9VM24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQTPSQQPP YSDDVFEPTQ LSQPAYSQTQ NTQTFISSQV EPRRKYWAIF ISTHSDRGIL KVPWSKPYIQ LGRGPYTLGN DVILPEKRVS NTHCRFTLGM QGTKNLELAN DITKAWKEGE GEPEVWVEDL KSSNGTFVNG ARINTRRLLR HGDEISLGHA GTLDYHDVRY IYRSVGGKRL KNGQASSNSG DLEPVGQVYE KYQLLDRLGK GTFAEVHKAV DVETGNMRAI KQIVKHRFAG NDKTLQLFHR EINITRSLEH ENICRLIDWY EDPQHICLVL EYVDGGDLLD YIMNWPDEQG GLPEQHAAEL TVQICRAMAY THSKGITHRD LKPENILLTK EHEGARIVKI ADFGLAKMIH TNTMLVSMVG TPQYLAPEIV MQTDQQPGYE NVVDSWSVGI IVYSMMTKAL PFDEDSKLPV EQRIRARFNQ PPDVYLLEQR NVSQQAIDFI LRLLDKDPAK RMTMAQALDH DWLSEPSSQP VESQRMGLGG DSMWSIQSFD SLQPEFNYGP GNTTDWQRPA TVSGTRLESG IEGSDDFSQP MTKLHLNTPE VCNIPSQRAS SKQTTGAAGI NTPVSPLSPF VAGNPVNVVS PSVPASTSSA SLSKQQKRKL EHEKRANSIG SEDEGLEDIN RTNNDPVNEN IDASIMKESA VDSVKEDGKG DIVLARRPRK SMRLQ
NT Sequence
atgtctcaaa ctccttccca gcagcccccc tacagtgacg atgtctttga acccacccag ctgtctcaac cagcatacag ccaaacccaa aacacacaga cgttcatcag cagccaggta gagccgcgca gaaaatactg ggcgatcttc atctctaccc actcggatcg cggaatctta aaagtgccct ggtccaagcc ctatattcaa ctgggaagag gaccatatac tcttggaaac gatgttatac ttcccgaaaa gagagtctcc aacacgcact gccgctttac tcttgggatg caaggtacga agaatctaga actagctaat gacatcacca aggcatggaa agaaggggag ggtgagcccg aagtatgggt agaggatttg aagagcagta acggaacgtt tgttaatggt gctcgtatca acactaggcg ccttttacgg catggtgatg agatatcact aggtcatgcg ggcactcttg actaccacga cgttcgatat atataccgtt ccgttggggg gaagagatta aagaatgggc aagcctcaag caatagtggc gacctcgagc ctgtaggcca ggtttacgag aagtatcaat tgctcgatcg cttggggaaa gggacatttg cagaagttca taaagctgtg gacgttgaaa cggggaatat gcgagccatc aaacaaattg tcaaacatcg ctttgctggc aatgacaaga ctctccagct gtttcaccga gaaatcaata tcactagaag tctggaacac gaaaacatat gtcggctgat tgattggtat gaagatcctc aacatatatg tcttgtcctg gagtatgtag atggaggtga tcttttggat tacatcatga actggccaga cgagcaaggc ggtctccctg agcaacacgc tgctgaactc acagttcaga tttgccgtgc aatggcctac acacattcaa aaggcatcac tcatagagac ttgaagccag agaatattct tttgacaaag gaacatgaag gcgctcgaat agtcaaaatt gcagattttg gcttggctaa gatgatccac acaaacacta tgcttgtatc catggtcggg actccgcagt atttggctcc tgagattgtc atgcaaacag atcagcaacc aggatacgaa aacgtggtgg actcctggtc tgtcggcata atagtttaca gcatgatgac caaggcttta ccatttgatg aggatagcaa acttcctgtt gagcaacgaa ttcgagcccg ttttaatcaa ccacctgatg tgtacctgtt ggagcagcga aacgtgagcc agcaagcaat cgatttcatt ttgaggcttt tggacaaaga tcccgccaag cgcatgacga tggcccaagc cttggatcat gattggcttt ctgagccctc ttcacagcca gttgagtcgc aacgcatggg tcttggtggt gattctatgt ggtctatcca atctttcgac tcattacaac ccgagtttaa ttatggtcca ggaaatacca cagactggca aagacccgcc acggtctctg gcacaaggtt agaaagtggt attgaggggt cagatgattt ctctcaacca atgaccaaac tgcatctcaa tactccagaa gtatgcaata taccttctca aagggcaagt agcaaacaaa caaccggcgc tgcaggcatc aacaccccag tatcacctct ctctcccttt gttgctggta acccggtcaa cgtcgtttca ccgtctgttc ctgcctcgac atcatccgca tctctttcaa aacaacaaaa acgcaagtta gaacatgaaa agagggctaa tagcatcggc tcggaagatg aaggtttaga ggatatcaac aggacaaata acgacccagt gaatgagaac attgacgcat ccattatgaa ggagtcggcg gtcgatagtg taaaggagga tggaaaaggc gatattgtat tggctagacg cccccgaaaa tctatgcgcc ttcaatag
Details for CrneC.00424.c.B32.GE41806
HARVESTED ON: data unavailable
SEQUENCED ON: 9/20/2017
EXPECTED MW: 38kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMG TLDYHDVRYI YRSVGGKRLK NGQASSNSGD LEPVGQVYEK YQLLDRLGKG TFAEVHKAVD VETGNMRAIK QIVKHRFAGN DKTLQLFHRE INITRSLEHE NICRLIDWYE DPQHICLVLE YVDGGDLLDY IMNWPDEQGG LPEQHAAELT VQICRAMAYT HSKGITHRDL KPENILLTKE HEGARIVKIA DFGLAKMIHT NTMLVSMVGT PQYLAPEIVM QTDQQPGYEN VVDSWSVGII VYSMMTKALP FDEDSKLPVE QRIRARFNQP PDVYLLEQRN VSQQAIDFIL GFWTKIPPSA
Validated NT Sequence
agggctcaga aagccaatca tgatccaagg cttgggccat cgtcatgcgc ttggcgggat ctttgtccaa aagcccaaaa tgaaatcgat tgcttgctgg ctcacgtttc gctgctccaa caggtacaca tcaggtggtt gattaaaacg ggctcgaatt cgttgctcaa caggaagttt gctatcctca tcaaatggta aagccttggt catcatgctg taaactatta tgccgacaga ccaggagtcc accacgtttt cgtatcctgg ttgctgatct gtttgcatga caatctcagg agccaaatac tgcggagtcc cgaccatgga tacaagcata gtgtttgtgt ggatcatctt agccaagcca aaatctgcaa ttttgactat tcgagcgcct tcatgttcct ttgtcaaaag aatattctct ggcttcaagt ctctatgagt gatgcctttt gaatgtgtgt aggccattgc acggcaaatc tgaactgtga gttcagcagc gtgttgctca gggagaccgc cttgctcgtc tggccagttc atgatgtaat ccaaaagatc acctccatct acatactcca ggacaagaca tatatgttga ggatcttcat accaatcaat cagccgacat atgttttcgt gttccagact tctagtgata ttgatttctc ggtgaaacag ctggagagtc ttgtcattgc cagcaaagcg atgtttgaca atttgtttga tggctcgcat attccccgtt tcaacgtcca cagctttatg aacttctgca aatgtccctt tccccaagcg atcgagcaat tgatacttct cgtaaacctg gcctacaggc tcgaggtcgc cactattgct tgaggcttgc ccattcttta atctcttccc cccaacggaa cggtatatat atcgaacgtc gtggtagtca agagtgccca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHGT LDYHDVRYIY RSVGGKRLKN GQASSNSGDL EPVGQVYEKY QLLDRLGKGT FAEVHKAVDV ETGNMRAIKQ IVKHRFAGND KTLQLFHREI NITRSLEHEN ICRLIDWYED PQHICLVLEY VDGGDLLDYI MNWPDEQGGL PEQHAAELTV QICRAMAYTH SKGITHRDLK PENILLTKEH EGARIVKIAD FGLAKMIHTN TMLVSMVGTP QYLAPEIVMQ TDQQPGYENV VDSWSVGIIV YSMMTKALPF DEDSKLPVEQ RIRARFNQPP DVYLLEQRNV SQQAIDFILR LLDKDPAKRM TMAQALDHDW LSEPSSQPVE SQR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcactc ttgactacca cgacgttcga tatatatacc gttccgttgg ggggaagaga ttaaagaatg ggcaagcctc aagcaatagt ggcgacctcg agcctgtagg ccaggtttac gagaagtatc aattgctcga tcgcttgggg aaagggacat ttgcagaagt tcataaagct gtggacgttg aaacggggaa tatgcgagcc atcaaacaaa ttgtcaaaca tcgctttgct ggcaatgaca agactctcca gctgtttcac cgagaaatca atatcactag aagtctggaa cacgaaaaca tatgtcggct gattgattgg tatgaagatc ctcaacatat atgtcttgtc ctggagtatg tagatggagg tgatcttttg gattacatca tgaactggcc agacgagcaa ggcggtctcc ctgagcaaca cgctgctgaa ctcacagttc agatttgccg tgcaatggcc tacacacatt caaaaggcat cactcataga gacttgaagc cagagaatat tcttttgaca aaggaacatg aaggcgctcg aatagtcaaa attgcagatt ttggcttggc taagatgatc cacacaaaca ctatgcttgt atccatggtc gggactccgc agtatttggc tcctgagatt gtcatgcaaa cagatcagca accaggatac gaaaacgtgg tggactcctg gtctgtcggc ataatagttt acagcatgat gaccaaggct ttaccatttg atgaggatag caaacttcct gttgagcaac gaattcgagc ccgttttaat caaccacctg atgtgtacct gttggagcag cgaaacgtga gccagcaagc aatcgatttc attttgaggc ttttggacaa agatcccgcc aagcgcatga cgatggccca agccttggat catgattggc tttctgagcc ctcttcacag ccagttgagt cgcaacgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat