SSGCID: Target Page

CrneC.00268.a

Atypical/RIO/RIO2 protein kinase

CENTER ID:	CrneC.00268.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.00268.a.B3.GE41176	Truncation 2		1	356

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.00268.a.B3.PW38245	Truncation 2		1	356

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_01687
UniProt:	J9VWY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLDATDLRY ISADEFRVLT AVEMGSKNHE VVPSQLVAEL AQIRGGNVKK ALGDLAKRNL VARVQNVKYD GFRLTYGGLD YLALRTFSRR KPPSVHSVGT RIGVGKESDI YVVADERGEK RVLKMHRLGR ISFRAIKSKR DYLGKRKSAS WMYMSRLSAQ KEFAFMKALY QHGFPVPVPI DQARHCVVMS LVDGYPLRSV EAVDDPADLY SKLMELIVRL AHAGLIHGDF NEFNVMLLRK TGEPVVIDFP QMVSTRHENA EYFFSRDVNC IRRFFRRRFR YEATSWPTWK NVLEIETGNA ENAVQGGVND GLTDLPVAQN RVRIDLEVEA SGFGRALQRE LEDYMIEVQD IPGSESDEEE DGEEKEEEEE EEEEEEEEEE EEEEEEEEEE ALSSHVQGND EKELSDEAAV AARLEALRLH RALGNDVSDE DADPDSEHSL TDFTSDDDSD DSDNSTGVAQ TDFTTYTPSM RNQPRHHTRL PVKEFENRGG ARSAIAKQVE RERHQTERKV NAGKTSVKLG KQKGHKWKSS DKYVVGKDSG W

NT Sequence

atgaaattag acgccaccga tctaagatac atttctgcgg atgagtttcg cgttctcact gctgttgaga tgggttctaa aaatcacgaa gtagttccct cgcagcttgt tgccgagttg gcccagattc gtggggggaa tgtcaagaaa gccttaggcg accttgcaaa acgtaacctt gtggctagag tacaaaatgt caaatatgat ggattcaggt tgacttatgg tggtttggac tatcttgcct tgaggacctt ctctcgcaga aagccaccca gtgttcattc agtggggact agaattggtg tcggcaaaga aagcgatatt tatgtcgtcg cagatgagcg cggtgaaaaa agggtattga agatgcatag gttgggaaga atcagtttca gagctataaa gagcaaacga gactatcttg ggaagaggaa gagtgcttct tggatgtaca tgagcagact ttcagctcag aaggaattcg cttttatgaa agcactttat caacatggct ttcctgtacc agtccccatc gatcaggccc gtcattgcgt tgtcatgtct ctggttgatg gctacccatt acggtctgtc gaagccgtgg acgatcccgc ggatttatac tctaaactga tggagcttat tgtccgttta gcccatgctg gcttaattca tggagacttc aatgagttca acgtcatgct tttgaggaag actggggagc ccgtcgtgat tgactttcct caaatggtca gcactagaca tgagaatgcg gaatactttt tcagtcgtga cgtgaattgt attcgtcgtt tcttcagaag gcgtttcaga tatgaagcta cttcttggcc aacctggaag aatgttttgg aaatcgaaac tggaaatgct gagaatgctg tacaaggggg ggtaaacgac ggtctaacgg atcttccggt ggcccagaat cgtgtgagga tcgacttgga agtggaggcc agtggcttcg ggcgtgctct gcaacgcgag cttgaggact atatgattga agtgcaagat attcccggca gtgagagtga tgaagaagaa gacggagaag aaaaggaaga ggaggaagag gaggaagagg aggaagagga ggaagaggag gaagaggagg aagaggagga agaggaggag gccttatcat cacacgttca aggcaatgat gaaaaagagc tttctgacga agctgctgtg gcggctcgtc tggaagcact ccgccttcat cgcgccttgg gtaatgatgt cagcgacgaa gatgccgacc ccgattcgga acactccctt actgacttca cgtctgacga tgactcagat gattcggata attccacggg tgttgctcag acagacttca caacatacac accctcaatg cgtaatcaac ctcgtcatca tacgagatta ccggtgaagg agttcgaaaa tagaggggga gcaaggagtg ccattgcaaa acaggtagag agagagagac atcagacgga gaggaaggtc aatgcgggta aaacaagtgt gaagttgggt aagcagaagg gtcataaatg gaagagcagt gataagtacg tagttgggaa ggatagtgga tggtga

Details for CrneC.00268.a.B3.GE41176

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	41kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK LDATDLRYIS ADEFRVLTAV EMGSKNHEVV PSQLVAELAQ IRGGNVKKAL GDLAKRNLVA RVQNVKYDGF RLTYGGLDYL ALRTFSRRKP PSVHSVGTRI GVGKESDIYV VADERGEKRV LKMHRLGRIS FRAIKSKRDY LGKRKSASWM YMSRLSAQKE FAFMKALYQH GFPVPVPIDQ ARHCVVMSLV DGYPLRSVEA VDDPADLYSK LMELIVRLAH AGLIHGDFNE FNVMLLRKTG EPVVIDFPQM VSTRHENAEY FFSRDVNCIR RFFRRRFRYE ATSWPTWKNV LEIETGNAEN AVQGGVNDGL TDLPVAQNRV RIDLEVEASG FGRALQRELE DYMIEVQDIP GSES

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat tagacgccac cgatctaaga tacatttctg cggatgagtt tcgcgttctc actgctgttg agatgggttc taaaaatcac gaagtagttc cctcgcagct tgttgccgag ttggcccaga ttcgtggggg gaatgtcaag aaagccttag gcgaccttgc aaaacgtaac cttgtggcta gagtacaaaa tgtcaaatat gatggattca ggttgactta tggtggtttg gactatcttg ccttgaggac cttctctcgc agaaagccac ccagtgttca ttcagtgggg actagaattg gtgtcggcaa agaaagcgat atttatgtcg tcgcagatga gcgcggtgaa aaaagggtat tgaagatgca taggttggga agaatcagtt tcagagctat aaagagcaaa cgagactatc ttgggaagag gaagagtgct tcttggatgt acatgagcag actttcagct cagaaggaat tcgcttttat gaaagcactt tatcaacatg gctttcctgt accagtcccc atcgatcagg cccgtcattg cgttgtcatg tctctggttg atggctaccc attacggtct gtcgaagccg tggacgatcc cgcggattta tactctaaac tgatggagct tattgtccgt ttagcccatg ctggcttaat tcatggagac ttcaatgagt tcaacgtcat gcttttgagg aagactgggg agcccgtcgt gattgacttt cctcaaatgg tcagcactag acatgagaat gcggaatact ttttcagtcg tgacgtgaat tgtattcgtc gtttcttcag aaggcgtttc agatatgaag ctacttcttg gccaacctgg aagaatgttt tggaaatcga aactggaaat gctgagaatg ctgtacaagg gggggtaaac gacggtctaa cggatcttcc ggtggcccag aatcgtgtga ggatcgactt ggaagtggag gccagtggct tcgggcgtgc tctgcaacgc gagcttgagg actatatgat tgaagtgcaa gatattcccg gcagtgagag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for CrneC.00268.a.B3.PW38245

PURIFICATION DATe:	4/10/2017
CONCENTRATION:	30mg/ml
OBSERVED MW:	41kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT TAGACGCCAC CGATCTAAGA TACATTTCTG CGGATGAGTT TCGCGTTCTC ACTGCTGTTG AGATGGGTTC TAAAAATCAC GAAGTAGTTC CCTCGCAGCT TGTTGCCGAG TTGGCCCAGA TTCGTGGGGG GAATGTCAAG AAAGCCTTAG GCGACCTTGC AAAACGTAAC CTTGTGGCTA GAGTACAAAA TGTCAAATAT GATGGATTCA GGTTGACTTA TGGTGGTTTG GACTATCTTG CCTTGAGGAC CTTCTCTCGC AGAAAGCCAC CCAGTGTTCA TTCAGTGGGG ACTAGAATTG GTGTCGGCAA AGAAAGCGAT ATTTATGTCG TCGCAGATGA GCGCGGTGAA AAAAGGGTAT TGAAGATGCA TAGGTTGGGA AGAATCAGTT TCAGAGCTAT AAAGAGCAAA CGAGACTATC TTGGGAAGAG GAAGAGTGCT TCTTGGATGT ACATGAGCAG ACTTTCAGCT CAGAAGGAAT TCGCTTTTAT GAAAGCACTT TATCAACATG GCTTTCCTGT ACCAGTCCCC ATCGATCAGG CCCGTCATTG CGTTGTCATG TCTCTGGTTG ATGGCTACCC ATTACGGTCT GTCGAAGCCG TGGACGATCC CGCGGATTTA TACTCTAAAC TGATGGAGCT TATTGTCCGT TTAGCCCATG CTGGCTTAAT TCATGGAGAC TTCAATGAGT TCAACGTCAT GCTTTTGAGG AAGACTGGGG AGCCCGTCGT GATTGACTTT CCTCAAATGG TCAGCACTAG ACATGAGAAT GCGGAATACT TTTTCAGTCG TGACGTGAAT TGTATTCGTC GTTTCTTCAG AAGGCGTTTC AGATATGAAG CTACTTCTTG GCCAACCTGG AAGAATGTTT TGGAAATCGA AACTGGAAAT GCTGAGAATG CTGTACAAGG GGGGGTAAAC GACGGTCTAA CGGATCTTCC GGTGGCCCAG AATCGTGTGA GGATCGACTT GGAAGTGGAG GCCAGTGGCT TCGGGCGTGC TCTGCAACGC GAGCTTGAGG ACTATATGAT TGAAGTGCAA GATATTCCCG GCAGTGAGAG TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT