SSGCID: Target Page

CrneC.00232.a

Glycerol kinase

CENTER ID:	CrneC.00232.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.00232.a.B3.GE41169	Truncation 2		90	740
CrneC.00232.a.B4.GE41170	Truncation 3		135	761

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_01155
UniProt:	J9VTF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRPIDLSMS SVKNPSPLAA GGTGGFTPVF EASPANSPAG SRRPSISQPA ARRPSEVSVS RRPSVNQQYS SYMSQYPQQG VQSLSRRSSM ASGPRPIRRG EYSGPATPSV LSRAGSPTLP LGNEFTQAPR SYFEGASGYT PLGGTRELRN GQFIGSLDCG TTSTRFIIFD KRAKIIAEHQ TEFEQILPHA GWHEHDPEAL VEAMNECIIR AVEKLEWMGW SRNSIKGIGI TNQRETTVCW SRSTGKPLCN AIVWDDARTI NVVREMERKL DEEGLEIDDE EEDEINQKSE IVPDKVEMGT GTHEAAFGDK GDVVDGDTSM TGRIGKAMEG LGLAGRGKDG KAKKYRKGKE GLVDVTGIPL STYFSAIKLR WMLDHQKAVR VAHENDDLMF GTVDTWLVYN LTGAKNGGLH IIDVTNASRT LLVSLKTLRW HAPLLRFFGL RASILPKIVS SAEVYGNISD SLGLPLSGVP IAGIVGDQQA ALVGNKCLMK GQAKCTFGTG AFVLFNTGED IVRSNYGLIS TVAFQAGADS KPIYALEGSI AVAGSAIKWL RDQMTLIEES SEMDILAGSV SDTGGVYFVT AFSGLLAPYW DREAAGTIIG LTGYTTSAHI ARATLEAVCF QTRAVLDVIE KESGSRLDTL KVDGGVTNSD LAMQLQANIG GFNVARPSMR ESTALGSALL AAHALGLFGW DVTRPETLSE VNTAGVHTFE PELEEKARLK KIKGWNKAVD RAKKWHDIED EDEEEEEYEN EVGYARIAGS A

NT Sequence

atgtctcgcc ccatagacct ttctatgtcc tcggtcaaga acccctcgcc ccttgcggca ggcggtactg gcggcttcac ccctgtcttc gaggcgtccc ccgccaattc tcccgcaggt tcccgtcgac cttctatctc acagcctgca gctcgtcgtc cttcagaggt ctcggtttcg cgtaggcctt ctgtgaatca acaatacagc agctacatgt cgcagtatcc tcaacaaggc gtccagtctc tttcccgacg atcatctatg gcttccggcc cgcgacctat ccgtcggggc gaatactctg gacctgccac cccgtctgtc ctatctcgag ctggttcgcc caccttgccc ctcggtaacg agttcactca agctccaagg tcatactttg agggtgcttc aggctacact cctttaggcg gaacgcggga gttgcgtaat gggcaattta ttggtagtct cgattgcggt actacctcta cgcgattcat catttttgat aagcgagcaa agattatcgc agagcatcaa accgaattcg aacagattct ccctcatgca ggttggcatg agcatgatcc cgaagcgctg gttgaagcga tgaacgaatg tattatcaga gctgttgaaa agctcgaatg gatgggctgg tccagaaata gcatcaaagg tattggtatc accaaccaac gagagacgac tgtctgctgg tcacgttcta ctggcaagcc tctttgtaat gccatcgttt gggatgatgc gcgaactatc aatgtggttc gtgagatgga aaggaagctt gacgaggaag gcctggaaat tgatgacgag gaggaggacg agatcaatca aaaatctgaa attgtgccag acaaagttga gatgggaact ggtacccatg aggcagcctt cggtgacaaa ggtgatgtcg tagatggtga taccagtatg accggcagaa ttggcaaagc gatggagggc ctcggtctgg cgggcagagg caaagatgga aaggcgaaga agtacagaaa gggcaaggaa ggtctggtgg acgttaccgg tattcctctc tctacctact tttctgccat caaacttcga tggatgttgg atcatcaaaa ggcagtccga gtcgcccatg aaaatgatga tctgatgttc ggcaccgttg atacttggct tgtctataat ttgaccggtg ccaagaatgg cggtctccat attattgacg ttaccaatgc ctcccgaaca ctccttgtct ctcttaaaac acttcgatgg cacgctcctc tccttcgctt cttcggcctt cgagcttcga tccttcccaa gatcgtgtct tcggccgaag tgtacggcaa tatctctgat tcactcggcc taccactctc cggtgttcca attgctggta ttgttggcga tcagcaggcc gctctggtcg gcaacaaatg tttgatgaag ggccaggcca agtgcacttt tggtactggt gctttcgtct tattcaacac tggcgaagac attgtcagga gtaactatgg tttaatttct actgtagcct ttcaagcagg tgcagacagt aagcctatat atgcactgga aggttctatt gccgtggccg gatcggccat aaaatggcta cgagaccaaa tgaccctcat tgaagaatct tccgaaatgg acatcctcgc cggatcagta tcggacactg gaggtgtcta ttttgttacc gccttctctg gcctccttgc gccgtactgg gatcgtgaag ctgccggtac tatcatcggt cttactggat acacaacttc tgctcacatt gcccgggcca cccttgaggc cgtgtgtttc caaactcgcg cggttttaga tgtcatcgag aaggagagcg gtagcaggct cgacactctc aaggttgatg gtggtgtgac caactcggac ttggctatgc agttgcaagc taacattggt ggcttcaacg ttgcacgacc ttcgatgcgc gagtccactg cgcttggttc cgccttgctg gctgctcatg ctcttggcct gtttggttgg gatgtgaccc gtccagaaac tctcagtgag gtgaacaccg ctggcgttca cacattcgag cccgagttgg aggagaaagc cagactgaag aagatcaagg gatggaacaa ggctgttgac agggccaaaa aatggcatga cattgaggat gaggacgaag aagaagaaga atacgaaaac gaagtcggat acgccaggat cgccgggtcg gcttga

Details for CrneC.00232.a.B3.GE41169

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	72kDa
OBSERVED MW:	72kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA SGPRPIRRGE YSGPATPSVL SRAGSPTLPL GNEFTQAPRS YFEGASGYTP LGGTRELRNG QFIGSLDCGT TSTRFIIFDK RAKIIAEHQT EFEQILPHAG WHEHDPEALV EAMNECIIRA VEKLEWMGWS RNSIKGIGIT NQRETTVCWS RSTGKPLCNA IVWDDARTIN VVREMERKLD EEGLEIDDEE EDEINQKSEI VPDKVEMGTG THEAAFGDKG DVVDGDTSMT GRIGKAMEGL GLAGRGKDGK AKKYRKGKEG LVDVTGIPLS TYFSAIKLRW MLDHQKAVRV AHENDDLMFG TVDTWLVYNL TGAKNGGLHI IDVTNASRTL LVSLKTLRWH APLLRFFGLR ASILPKIVSS AEVYGNISDS LGLPLSGVPI AGIVGDQQAA LVGNKCLMKG QAKCTFGTGA FVLFNTGEDI VRSNYGLIST VAFQAGADSK PIYALEGSIA VAGSAIKWLR DQMTLIEESS EMDILAGSVS DTGGVYFVTA FSGLLAPYWD REAAGTIIGL TGYTTSAHIA RATLEAVCFQ TRAVLDVIEK ESGSRLDTLK VDGGVTNSDL AMQLQANIGG FNVARPSMRE STALGSALLA AHALGLFGWD VTRPETLSEV NTAGVHTFEP ELEEKARLKK IKGWNKAVDR AKKWHDIED

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctt ccggcccgcg acctatccgt cggggcgaat actctggacc tgccaccccg tctgtcctat ctcgagctgg ttcgcccacc ttgcccctcg gtaacgagtt cactcaagct ccaaggtcat actttgaggg tgcttcaggc tacactcctt taggcggaac gcgggagttg cgtaatgggc aatttattgg tagtctcgat tgcggtacta cctctacgcg attcatcatt tttgataagc gagcaaagat tatcgcagag catcaaaccg aattcgaaca gattctccct catgcaggtt ggcatgagca tgatcccgaa gcgctggttg aagcgatgaa cgaatgtatt atcagagctg ttgaaaagct cgaatggatg ggctggtcca gaaatagcat caaaggtatt ggtatcacca accaacgaga gacgactgtc tgctggtcac gttctactgg caagcctctt tgtaatgcca tcgtttggga tgatgcgcga actatcaatg tggttcgtga gatggaaagg aagcttgacg aggaaggcct ggaaattgat gacgaggagg aggacgagat caatcaaaaa tctgaaattg tgccagacaa agttgagatg ggaactggta cccatgaggc agccttcggt gacaaaggtg atgtcgtaga tggtgatacc agtatgaccg gcagaattgg caaagcgatg gagggcctcg gtctggcggg cagaggcaaa gatggaaagg cgaagaagta cagaaagggc aaggaaggtc tggtggacgt taccggtatt cctctctcta cctacttttc tgccatcaaa cttcgatgga tgttggatca tcaaaaggca gtccgagtcg cccatgaaaa tgatgatctg atgttcggca ccgttgatac ttggcttgtc tataatttga ccggtgccaa gaatggcggt ctccatatta ttgacgttac caatgcctcc cgaacactcc ttgtctctct taaaacactt cgatggcacg ctcctctcct tcgcttcttc ggccttcgag cttcgatcct tcccaagatc gtgtcttcgg ccgaagtgta cggcaatatc tctgattcac tcggcctacc actctccggt gttccaattg ctggtattgt tggcgatcag caggccgctc tggtcggcaa caaatgtttg atgaagggcc aggccaagtg cacttttggt actggtgctt tcgtcttatt caacactggc gaagacattg tcaggagtaa ctatggttta atttctactg tagcctttca agcaggtgca gacagtaagc ctatatatgc actggaaggt tctattgccg tggccggatc ggccataaaa tggctacgag accaaatgac cctcattgaa gaatcttccg aaatggacat cctcgccgga tcagtatcgg acactggagg tgtctatttt gttaccgcct tctctggcct ccttgcgccg tactgggatc gtgaagctgc cggtactatc atcggtctta ctggatacac aacttctgct cacattgccc gggccaccct tgaggccgtg tgtttccaaa ctcgcgcggt tttagatgtc atcgagaagg agagcggtag caggctcgac actctcaagg ttgatggtgg tgtgaccaac tcggacttgg ctatgcagtt gcaagctaac attggtggct tcaacgttgc acgaccttcg atgcgcgagt ccactgcgct tggttccgcc ttgctggctg ctcatgctct tggcctgttt ggttgggatg tgacccgtcc agaaactctc agtgaggtga acaccgctgg cgttcacaca ttcgagcccg agttggagga gaaagccaga ctgaagaaga tcaagggatg gaacaaggct gttgacaggg ccaaaaaatg gcatgacatt gaggattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for CrneC.00232.a.B4.GE41170

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	69kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHGA SGYTPLGGTR ELRNGQFIGS LDCGTTSTRF IIFDKRAKII AEHQTEFEQI LPHAGWHEHD PEALVEAMNE CIIRAVEKLE WMGWSRNSIK GIGITNQRET TVCWSRSTGK PLCNAIVWDD ARTINVVREM ERKLDEEGLE IDDEEEDEIN QKSEIVPDKV EMGTGTHEAA FGDKGDVVDG DTSMTGRIGK AMEGLGLAGR GKDGKAKKYR KGKEGLVDVT GIPLSTYFSA IKLRWMLDHQ KAVRVAHEND DLMFGTVDTW LVYNLTGAKN GGLHIIDVTN ASRTLLVSLK TLRWHAPLLR FFGLRASILP KIVSSAEVYG NISDSLGLPL SGVPIAGIVG DQQAALVGNK CLMKGQAKCT FGTGAFVLFN TGEDIVRSNY GLISTVAFQA GADSKPIYAL EGSIAVAGSA IKWLRDQMTL IEESSEMDIL AGSVSDTGGV YFVTAFSGLL APYWDREAAG TIIGLTGYTT SAHIARATLE AVCFQTRAVL DVIEKESGSR LDTLKVDGGV TNSDLAMQLQ ANIGGFNVAR PSMRESTALG SALLAAHALG LFGWDVTRPE TLSEVNTAGV HTFEPELEEK ARLKKIKGWN KAVDRAKKWH DIEDEDEEEE EYENEVGYAR IAGSA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtgctt caggctacac tcctttaggc ggaacgcggg agttgcgtaa tgggcaattt attggtagtc tcgattgcgg tactacctct acgcgattca tcatttttga taagcgagca aagattatcg cagagcatca aaccgaattc gaacagattc tccctcatgc aggttggcat gagcatgatc ccgaagcgct ggttgaagcg atgaacgaat gtattatcag agctgttgaa aagctcgaat ggatgggctg gtccagaaat agcatcaaag gtattggtat caccaaccaa cgagagacga ctgtctgctg gtcacgttct actggcaagc ctctttgtaa tgccatcgtt tgggatgatg cgcgaactat caatgtggtt cgtgagatgg aaaggaagct tgacgaggaa ggcctggaaa ttgatgacga ggaggaggac gagatcaatc aaaaatctga aattgtgcca gacaaagttg agatgggaac tggtacccat gaggcagcct tcggtgacaa aggtgatgtc gtagatggtg ataccagtat gaccggcaga attggcaaag cgatggaggg cctcggtctg gcgggcagag gcaaagatgg aaaggcgaag aagtacagaa agggcaagga aggtctggtg gacgttaccg gtattcctct ctctacctac ttttctgcca tcaaacttcg atggatgttg gatcatcaaa aggcagtccg agtcgcccat gaaaatgatg atctgatgtt cggcaccgtt gatacttggc ttgtctataa tttgaccggt gccaagaatg gcggtctcca tattattgac gttaccaatg cctcccgaac actccttgtc tctcttaaaa cacttcgatg gcacgctcct ctccttcgct tcttcggcct tcgagcttcg atccttccca agatcgtgtc ttcggccgaa gtgtacggca atatctctga ttcactcggc ctaccactct ccggtgttcc aattgctggt attgttggcg atcagcaggc cgctctggtc ggcaacaaat gtttgatgaa gggccaggcc aagtgcactt ttggtactgg tgctttcgtc ttattcaaca ctggcgaaga cattgtcagg agtaactatg gtttaatttc tactgtagcc tttcaagcag gtgcagacag taagcctata tatgcactgg aaggttctat tgccgtggcc ggatcggcca taaaatggct acgagaccaa atgaccctca ttgaagaatc ttccgaaatg gacatcctcg ccggatcagt atcggacact ggaggtgtct attttgttac cgccttctct ggcctccttg cgccgtactg ggatcgtgaa gctgccggta ctatcatcgg tcttactgga tacacaactt ctgctcacat tgcccgggcc acccttgagg ccgtgtgttt ccaaactcgc gcggttttag atgtcatcga gaaggagagc ggtagcaggc tcgacactct caaggttgat ggtggtgtga ccaactcgga cttggctatg cagttgcaag ctaacattgg tggcttcaac gttgcacgac cttcgatgcg cgagtccact gcgcttggtt ccgccttgct ggctgctcat gctcttggcc tgtttggttg ggatgtgacc cgtccagaaa ctctcagtga ggtgaacacc gctggcgttc acacattcga gcccgagttg gaggagaaag ccagactgaa gaagatcaag ggatggaaca aggctgttga cagggccaaa aaatggcatg acattgagga tgaggacgaa gaagaagaag aatacgaaaa cgaagtcgga tacgccagga tcgccgggtc ggcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat