CrneB.17228.a

Sre1 sterol regulatory element-binding protein

CENTER ID:	CrneB.17228.a
ORGANISM:	Cryptococcus neoformans var. neoformans JEC21
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneB.17228.a.A16.GE31667	full length		138	462

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneB.17228.a.A16.PW32996	full length		138	462

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNJ02310
OrthoMCL:	OG5_225902
RefSeq:	XP_567526.1
UniProt:	Q5KAB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPTSLYNTQF DQEEQDLIFA PSDVSPTDAL FPPELTALFS DNQFPLDNPS NNFGGNHQHR GSLSSNEDSL PFLSPTNPQS MSSTSPRLSL SPSTGEPSFH LTSHYSYDQS SHSPHSHSSA SPLSSSGTDD VFLARSFEGV SGGELDMFLL SEPINDYPVN SDGFGGQKPG QSLFLPGRTE GFNGMGDEWI QDLFKDPLQE NGLDNINQQP QPQPDGLDFD PILAGLQQRP QQQPLQQQKQ QFGQTIQPQM VKQEPLIKQD SWSNGQNLSN SQHGFTFTHP PFAPRNQAQD IKPASIPRTA SVRRPTASKS ETITAAAPAP TIGKHNKTER RYRQKVQAAQ ADLRDAIPAL RLLYGTSTPE QLATTDIRAP DGTVDGLGEV TRPNASAKAT ILIGARVYIE LLQKRSAKLQ RMVNELEKFR GAVEGEAGLA AWKEDFGRRE AEIDRVEAEQ LAAKLKEEED ESEGEDDDDD EEPSKKRKRT AAPKAKPKTA AKTKITPQST AAAGARVFAA FAMSFSFVPS ASTVFRSNPQ PQALATDKVL GYATNQQILA SVPLIIAEHT SRLLARSLPS ALVPLPTTLL EWTWRLVVAV VLAVVMRPII SKLTTKSDEK ARPGTVKGVL KDVVGIVSRK KAQKTEWERF AAGVVGRANN VSTLAKWHTI LHLNVTASSP YSLALLALLQ PECSLLRSPQ QLWLTAQSRV DGTTPPALVT VLGLPLHEAL LCLSSLPPTS APLSALAEQI TLIHVHDLYT RLFIHLVEAS TASPLSTTSL KSLLSALESH NLGANLKASA FDKEIKSVMQ GTQKGSVVHA LGLVLIGLWG IFTGPSSSAQ ASLAGALAAD QVSGATHGLN SVSALLELLY PGCAPVSQAP AFVNPLSPNA QKVDALALSI IEYLSLLLTA SRSTEATNMD REGRKEESLG VQRKVVKLRG VLNKTGWVGV ESQLDADDFE EVYDQPEEFT LGDEADKRDF RPHQLESERI HYERAKERLV DILARIGRRA AGRANGRDED SGLEGDLDEL

NT Sequence

atgccgacct cactctacaa tacccagttc gaccaagaag aacaagattt gatcttcgcg ccttctgatg tttccccgac agacgctctt tttcctcctg aactcactgc tctcttctca gacaaccagt tccccttaga taatccgtca aataactttg gagggaatca tcaacaccga ggttccctct cttcaaatga ggattcactt cctttcctgt cacccaccaa cccacagtca atgtcctcga cctcaccacg tctttccctt tctccatcaa caggtgaacc ttcctttcat ctcacatcac attattccta cgatcaatcg tcgcactctc cgcatagtca cagttccgct tcacctttat cgagttccgg aacggatgat gtttttctgg ctagaagctt tgaaggtgtg agcggtggcg aattggatat gttcctgctt tcagagccga tcaatgatta tccagtcaat tccgatgggt ttggaggaca gaaacctggg cagtctctgt ttttgcccgg ccggacagaa ggattcaatg ggatgggtga tgagtggatt caagacctct ttaaggaccc tcttcaagaa aatggtcttg acaatattaa tcaacaaccg cagccacagc ctgatggtct tgactttgat ccgatactag cagggctcca acaacggcct cagcaacaac cattgcagca acagaagcag cagtttgggc aaaccatcca gcctcagatg gtgaagcaag aacctctaat aaagcaggac agttggagca acgggcaaaa cttgagcaac tctcaacacg gtttcacttt tactcaccct ccatttgctc ccaggaacca ggctcaggat atcaaacccg cttctatccc tcgcactgcg agtgtgcgcc gtcccaccgc ctctaaatct gaaacaatta cagctgctgc ccctgctcct actattggaa agcacaacaa aactgagcgc cgttatcgcc agaaggtgca agctgctcaa gccgacttgc gggatgccat acctgcacta cgtctgttgt acgggacttc cactcctgaa cagcttgcca ctaccgatat tcgtgcacct gacgggacag ttgacggtct cggagaggtg actagaccga acgctagtgc gaaagccacc attttgatag gggcgagggt atatattgaa ctcttgcaaa aacgctcagc aaagcttcag aggatggtca atgagcttga gaaatttagg ggagcggtgg aaggtgaagc gggccttgcg gcttggaagg aagactttgg acgaagagaa gcagaaattg atagggtaga agctgaacaa ttagcggcca agctcaagga agaggaagat gagagtgaag gagaagatga tgatgacgac gaagaacctt ccaaaaagcg taaacgtacg gctgcgccaa aggccaaacc caaaacagca gccaagacca aaatcactcc tcagtcgact gccgctgctg gtgctcgagt gttcgcagca tttgctatgt cattctcttt cgtcccttct gcttccaccg tcttccgttc aaatccacag ccccaagctc tggcaaccga taaagtcctt gggtatgcga caaatcaaca aatccttgcc agtgtaccgt taatcattgc ggagcataca tcgcgcctcc ttgcccgtag tcttccgagc gcccttgttc ccctccccac aaccctgctc gagtggacat ggagacttgt tgtcgctgtt gttcttgctg tggtcatgcg tccgatcatc tcgaaattga ccaccaaatc ggacgagaag gctaggccag gtacagtaaa gggtgttcta aaagatgttg tcgggatcgt ttcgagaaag aaggcccaaa agactgaatg ggaaagattt gctgcaggtg tcgttggtcg agccaataat gtgtctacac tggcaaagtg gcacactatc ttacatctta atgtcaccgc gtcatcaccc tattctcttg cactccttgc acttcttcag cctgaatgtt ctcttctgcg ttcaccgcag caactctggc tcactgccca gtcacgtgtt gatggaacta cacctcccgc tttagttact gtgcttggat tgcccttgca cgaagccttg ctttgccttt cttctcttcc tcccacctct gcaccgcttt ccgcactcgc cgaacagatc actctcattc atgttcacga tctctacacc cgtttattca tccacctggt cgaagcttct accgcttccc ccctttccac cacgtcactc aaatccctcc tgtccgctct tgaatctcac aacttgggtg ccaacctcaa agcatccgct tttgacaagg aaatcaaaag cgtgatgcaa ggtactcaga agggatcagt tgtgcacgct ctcggcttgg tattgattgg actttggggg atctttacgg ggcctagttc ctcagctcaa gcatcccttg cgggggcact tgcagctgac caggtttcag gcgcaactca tgggctcaat tcagtttcag ctttgcttga actcctttat cccggctgtg cccctgtctc tcaagccccc gctttcgtca atcctctttc accaaacgct caaaaagtcg atgcccttgc actgtcaatc attgaatatc tttctcttct tctcaccgct tctcgttcca cagaggccac gaatatggac cgagaaggta ggaaagaaga aagtctcggt gttcagcgga aggtagtcaa gttgagagga gtattgaaca agactggatg ggtgggtgtg gaatctcagc tcgatgcgga tgactttgag gaagtttacg atcagccaga ggaattcaca ctaggagatg aagcggataa gcgtgatttc agacctcatc aacttgagag cgagagaata cattacgagc gggcgaagga gagactggta gacattcttg ccaggattgg caggcgggct gcaggaagag ctaatggaag agatgaagat tcgggactgg agggcgattt agatgaactg taa

Details for CrneB.17228.a.A16.GE31667

HARVESTED ON:	2/23/2011
SEQUENCED ON:	3/1/2011
EXPECTED MW:	38kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MEGVSGGELD MFLLSEPIND YPVNSDGFGG QKPGQSLFLP GRTEGFNGMG DEWIQDLFKD PLQENGLDNI NQQPQPQPDG LDFDPILAGL QQRPQQQPLQ QQKQQFGQTI QPQMVKQEPL IKQDSWSNGQ NLSNSQHGFT FTHPPFAPRN QAQDIKPASI PRTASVRRPT ASKSETITAA APAPTIGKHN KTERRYRQKV QAAQADLRDA IPALRLLYGT STPEQLATTD IRAPDGTVDG LGEVTRPNAS AKATILIGAR VYIELLQKRS AKLQRMVNEL EKFRGAVEGE AGLAAWKEDF GRREAEIDRV EAEQLAAKLK EEED

Validated NT Sequence

cgtcctcttc ctctttcagc tttgctgcca gctgttcagc ctcaacgcgg tcaatttcag cttcacgacg accgaaatct tctttccagg cagccagacc ggcctcgcct tcaacggcac cacggaactt ctccagttca ttaaccatac gctggagctt ggcagaacgt ttctggagca gttcgatgta aacgcgagca ccaatcagga tggtggcctt agcggaggcg ttcggacggg tcacttcacc cagaccgtca acagtaccat ccggagcacg gatgtcagtg gtggccagct gttccggagt gctagtaccg tagagcagac ggagagccgg aattgcgtca cgcaggtcag cctgtgcagc ctggaccttc tggcgataac gacgttcagt cttgttatgt ttaccaatag taggagctgg agctgcagca gtaatggttt cagacttgga agcggtaggg cgacgaacag aggcggtacg tgggatggat gcaggtttaa tgtcctgagc ctggttacgc ggagcaaatg gagggtgggt aaaggtgaag ccgtgctgag aattgctcag gttctggccg ttgctccaag aatcctgttt gatcagcggc tcctgtttca ccatctgcgg ctggatggtt tgaccgaatt gctgcttctg ttgctggagc ggctgttgct gtggacgctg ttggagacct gcgaggatcg gatcgaagtc aagaccgtca ggttgtggct gcggctgctg gttgatgtta tccaggccgt tctcctgcag tgggtcttta aacaggtcct gaatccattc gtcacccatg ccgttgaaac cctcggtgcg acccggcagg aagagagact ggcccggctt ctgaccaccg aaaccgtcag agttcaccgg gtaatcgttg ataggttcgc tcaggagaaa catatccagt tcgccgccag aaacgccctc catcgaacca ggaccctggg tctgagcttc cagggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEGVSGGEL DMFLLSEPIN DYPVNSDGFG GQKPGQSLFL PGRTEGFNGM GDEWIQDLFK DPLQENGLDN INQQPQPQPD GLDFDPILAG LQQRPQQQPL QQQKQQFGQT IQPQMVKQEP LIKQDSWSNG QNLSNSQHGF TFTHPPFAPR NQAQDIKPAS IPRTASVRRP TASKSETITA AAPAPTIGKH NKTERRYRQK VQAAQADLRD AIPALRLLYG TSTPEQLATT DIRAPDGTVD GLGEVTRPNA SAKATILIGA RVYIELLQKR SAKLQRMVNE LEKFRGAVEG EAGLAAWKED FGRREAEIDR VEAEQLAAKL KEEEDES

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagggcgtt tctggcggcg aactggatat gtttctcctg agcgaaccta tcaacgatta cccggtgaac tctgacggtt tcggtggtca gaagccgggc cagtctctct tcctgccggg tcgcaccgag ggtttcaacg gcatgggtga cgaatggatt caggacctgt ttaaagaccc actgcaggag aacggcctgg ataacatcaa ccagcagccg cagccacaac ctgacggtct tgacttcgat ccgatcctcg caggtctcca acagcgtcca cagcaacagc cgctccagca acagaagcag caattcggtc aaaccatcca gccgcagatg gtgaaacagg agccgctgat caaacaggat tcttggagca acggccagaa cctgagcaat tctcagcacg gcttcacctt tacccaccct ccatttgctc cgcgtaacca ggctcaggac attaaacctg catccatccc acgtaccgcc tctgttcgtc gccctaccgc ttccaagtct gaaaccatta ctgctgcagc tccagctcct actattggta aacataacaa gactgaacgt cgttatcgcc agaaggtcca ggctgcacag gctgacctgc gtgacgcaat tccggctctc cgtctgctct acggtactag cactccggaa cagctggcca ccactgacat ccgtgctccg gatggtactg ttgacggtct gggtgaagtg acccgtccga acgcctccgc taaggccacc atcctgattg gtgctcgcgt ttacatcgaa ctgctccaga aacgttctgc caagctccag cgtatggtta atgaactgga gaagttccgt ggtgccgttg aaggcgaggc cggtctggct gcctggaaag aagatttcgg tcgtcgtgaa gctgaaattg accgcgttga ggctgaacag ctggcagcaa agctgaaaga ggaagaggac gaatcttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for CrneB.17228.a.A16.PW32996

PURIFICATION DATe:	5/20/2011
CONCENTRATION:	42.94mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAGGGCGTT TCTGGCGGCG AACTGGATAT GTTTCTCCTG AGCGAACCTA TCAACGATTA CCCGGTGAAC TCTGACGGTT TCGGTGGTCA GAAGCCGGGC CAGTCTCTCT TCCTGCCGGG TCGCACCGAG GGTTTCAACG GCATGGGTGA CGAATGGATT CAGGACCTGT TTAAAGACCC ACTGCAGGAG AACGGCCTGG ATAACATCAA CCAGCAGCCG CAGCCACAAC CTGACGGTCT TGACTTCGAT CCGATCCTCG CAGGTCTCCA ACAGCGTCCA CAGCAACAGC CGCTCCAGCA ACAGAAGCAG CAATTCGGTC AAACCATCCA GCCGCAGATG GTGAAACAGG AGCCGCTGAT CAAACAGGAT TCTTGGAGCA ACGGCCAGAA CCTGAGCAAT TCTCAGCACG GCTTCACCTT TACCCACCCT CCATTTGCTC CGCGTAACCA GGCTCAGGAC ATTAAACCTG CATCCATCCC ACGTACCGCC TCTGTTCGTC GCCCTACCGC TTCCAAGTCT GAAACCATTA CTGCTGCAGC TCCAGCTCCT ACTATTGGTA AACATAACAA GACTGAACGT CGTTATCGCC AGAAGGTCCA GGCTGCACAG GCTGACCTGC GTGACGCAAT TCCGGCTCTC CGTCTGCTCT ACGGTACTAG CACTCCGGAA CAGCTGGCCA CCACTGACAT CCGTGCTCCG GATGGTACTG TTGACGGTCT GGGTGAAGTG ACCCGTCCGA ACGCCTCCGC TAAGGCCACC ATCCTGATTG GTGCTCGCGT TTACATCGAA CTGCTCCAGA AACGTTCTGC CAAGCTCCAG CGTATGGTTA ATGAACTGGA GAAGTTCCGT GGTGCCGTTG AAGGCGAGGC CGGTCTGGCT GCCTGGAAAG AAGATTTCGG TCGTCGTGAA GCTGAAATTG ACCGCGTTGA GGCTGAACAG CTGGCAGCAA AGCTGAAAGA GGAAGAGGAC GAATCTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA