SSGCID: Target Page

CrhoA.18370.a

phosphatidylinositol 3- and 4-kinase domain-containing protein

CENTER ID:	CrhoA.18370.a
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.18370.a.B1.GE35452	full length		1	472

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:Chro.80518
RefSeq:	XP_666044.1
UniProt:	Q5CGT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSFNNEFCS RFSENDYFNS NNKDGNSKGI IGFSHSDYIM DKGLCSLNNC QFLRIIPNEC KIYNSRKRVH FLLAIEIADL DDQDYELGKN SQDLISKDIL NYLKNHGIKL KTDNNDENQN RSQDILLNFL NILSVGISES IDMGLSQEDF NQITFIDNKD SDEIEDSSIH DNELSDEDVF INKDWPQETW SSKIKRYRKE SPYSKLKSWG IKTLLLKSSD DLRQEYLASQ LLQQFDWIFK INKLPLWLHP YEILVIGSHG GFIEYIQDTF SIDSLKRRFQ TDNLLNCFDQ LFKDEELKSK ARKCFVESHA AYSLVSYFLQ VKDRHNGNFL IDKHGHIIQI DYGFMLSNSP GNVNFEQSPF KLTQEFLDVM MGENSSDFQY FQQLIIKGFL ASRKHVDQIV LTIESMTSAS KLPCFSLFTN KEYFIQDIRD RFFLHLTEEQ CVVKVTELIQ SSINNWRSIQ YDAFQRITNG IL

NT Sequence

atgccatcat ttaataatga attttgtagt agattttcag aaaatgatta ttttaattca aataataaag atgggaatag taaaggtata attggatttt ctcactcaga ttatattatg gataaaggtt tatgttctct aaataattgc caatttttac gtataattcc aaatgaatgt aaaatttata attccagaaa aagagttcac tttttattag caattgaaat tgcagatctt gatgatcaag attatgagct tggaaagaat tctcaagatt taatttccaa ggatatttta aattatctaa aaaatcatgg aattaaactt aaaactgata ataatgatga aaatcaaaat agatcacaag atatattgct taattttctt aatatacttt ctgtcggtat ttcagaatca attgatatgg gtttatctca agaagacttt aaccaaataa cttttattga taataaagat tctgatgaga ttgaagattc tagtattcat gataatgaat taagtgatga agatgtattt attaataaag attggcctca agaaacttgg tcaagtaaga ttaagagata tagaaaagaa tcaccttact caaaattaaa gagttggggt attaaaacac ttttgttaaa atcctcagat gatcttagac aagaatatct tgcatctcaa ttacttcaac aatttgattg gatttttaaa attaataaat tacccttatg gcttcatcca tatgaaatac ttgtaattgg ttctcatggt ggatttattg aatatattca agatactttc tccatagata gccttaaacg tagattccag actgataatc ttttgaattg ctttgatcag ttatttaaag atgaggaact taaatctaaa gcaaggaaat gttttgttga aagtcatgct gcttatagcc ttgttagtta tttccttcaa gttaaagata gacataacgg taatttctta attgataagc atggtcatat tattcaaatt gattacgggt ttatgttaag caattctcct ggtaatgtaa attttgagca aagtcctttc aaattaactc aagaattcct tgacgtcatg atgggtgaaa actcttctga tttccaatat tttcaacaac ttattattaa aggattcttg gcttccagaa aacatgttga ccaaattgtt ttaaccattg aatctatgac ttcagcatct aaacttcctt gtttctcttt atttacaaac aaggaatact ttattcaaga tattagagat cgtttctttc ttcatcttac tgaggaacaa tgtgttgtta aagtcacaga attaattcaa tcttcaataa ataattggag atccattcaa tatgatgcat tccaacgaat taccaatgga attctttag

Details for CrhoA.18370.a.B1.GE35452

HARVESTED ON:	6/26/2012
SEQUENCED ON:	6/29/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	91
PERCENT COVERAGE:	58

Validated AA Sequence

Validated NT Sequence

attttgtnnn ntttaagaag gagatatacc atggctcacc accaccacca ccatatgcca tcatttaata atgaattttg tagtagattt tcagaaaatg attattttaa ttcaaataat aaagatggga atagtaaagg tataattgga ttttctcact cagattatat tatggataaa ggtttatgtt ctctaaataa ttgccaattt ttacgtataa ttccaaatga atgtaaaatt tataattcca gaaaaagagt tcacttttta ttagcaattg aaattgcaga tcttgatgat caagattatg agcttggaaa gaattctcaa gatttaattt ccaaggatat tttaaattat ctaaaaaatc atggaattaa acttaaaact gataataatg atgaaaatca aaatagatca caagatatat tgcttaattt tcttaatata ctttctgtcg gtatttcaga atcaattgat atgggtttat ctcaagaaga ctttaaccaa ataactttta ttgataataa agattctgat gagattgaag attctagtat tcatgataat gaattaagtg atgaagatgt atttgttaat aaagattggc ctcaagaaac ttggtcaagt aagattaaga gatatagaaa agaatcacct tactcaaaat taaagagttg gggtattaaa acacttttgt taaaatcctc agatgatctt agacaagaat atctngcatc tcaattactt caacaatttg attggntttt tnaaantaat aaattaccct natggcttcn tccntatgaa anactntant nnnntcangg ngnnttnntn nannnnntca nnananttnc tcnnnnntag nntnaacgta nnnnnnactg annnnnnttn nntncntnna tcnntntnnn natnnnnnta nnnnnnnnnn nnnanngntt tnnnnnannn cangctg

Expected Protein Sequence

MAHHHHHHMP SFNNEFCSRF SENDYFNSNN KDGNSKGIIG FSHSDYIMDK GLCSLNNCQF LRIIPNECKI YNSRKRVHFL LAIEIADLDD QDYELGKNSQ DLISKDILNY LKNHGIKLKT DNNDENQNRS QDILLNFLNI LSVGISESID MGLSQEDFNQ ITFIDNKDSD EIEDSSIHDN ELSDEDVFIN KDWPQETWSS KIKRYRKESP YSKLKSWGIK TLLLKSSDDL RQEYLASQLL QQFDWIFKIN KLPLWLHPYE ILVIGSHGGF IEYIQDTFSI DSLKRRFQTD NLLNCFDQLF KDEELKSKAR KCFVESHAAY SLVSYFLQVK DRHNGNFLID KHGHIIQIDY GFMLSNSPGN VNFEQSPFKL TQEFLDVMMG ENSSDFQYFQ QLIIKGFLAS RKHVDQIVLT IESMTSASKL PCFSLFTNKE YFIQDIRDRF FLHLTEEQCV VKVTELIQSS INNWRSIQYD AFQRITNGIL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccat catttaataa tgaattttgt agtagatttt cagaaaatga ttattttaat tcaaataata aagatgggaa tagtaaaggt ataattggat tttctcactc agattatatt atggataaag gtttatgttc tctaaataat tgccaatttt tacgtataat tccaaatgaa tgtaaaattt ataattccag aaaaagagtt cactttttat tagcaattga aattgcagat cttgatgatc aagattatga gcttggaaag aattctcaag atttaatttc caaggatatt ttaaattatc taaaaaatca tggaattaaa cttaaaactg ataataatga tgaaaatcaa aatagatcac aagatatatt gcttaatttt cttaatatac tttctgtcgg tatttcagaa tcaattgata tgggtttatc tcaagaagac tttaaccaaa taacttttat tgataataaa gattctgatg agattgaaga ttctagtatt catgataatg aattaagtga tgaagatgta tttattaata aagattggcc tcaagaaact tggtcaagta agattaagag atatagaaaa gaatcacctt actcaaaatt aaagagttgg ggtattaaaa cacttttgtt aaaatcctca gatgatctta gacaagaata tcttgcatct caattacttc aacaatttga ttggattttt aaaattaata aattaccctt atggcttcat ccatatgaaa tacttgtaat tggttctcat ggtggattta ttgaatatat tcaagatact ttctccatag atagccttaa acgtagattc cagactgata atcttttgaa ttgctttgat cagttattta aagatgagga acttaaatct aaagcaagga aatgttttgt tgaaagtcat gctgcttata gccttgttag ttatttcctt caagttaaag atagacataa cggtaatttc ttaattgata agcatggtca tattattcaa attgattacg ggtttatgtt aagcaattct cctggtaatg taaattttga gcaaagtcct ttcaaattaa ctcaagaatt ccttgacgtc atgatgggtg aaaactcttc tgatttccaa tattttcaac aacttattat taaaggattc ttggcttcca gaaaacatgt tgaccaaatt gttttaacca ttgaatctat gacttcagca tctaaacttc cttgtttctc tttatttaca aacaaggaat actttattca agatattaga gatcgtttct ttcttcatct tactgaggaa caatgtgttg ttaaagtcac agaattaatt caatcttcaa taaataattg gagatccatt caatatgatg cattccaacg aattaccaat ggaattcttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat