SSGCID: Target Page

CrhoA.18340.a

ribosomal RNA large subunit methyltransferase J, putative

CENTER ID:	CrhoA.18340.a
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.18340.a.B1.GE35458	full length		1	253

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:Chro.40181
RefSeq:	XP_665029.1
UniProt:	Q5CDW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNDNIQESFN QNGSDDEDMV EQMSFNSENS DTDEVQESDD ERFTKPENEE DLKIIQGIGS LLVNKSTRMD LIDGSYNRYA FHDPDEDLEN NVLGLPSWFI EDENKHNKPE LPITKELMAQ YRAKLREIKN RPIRKESEAL ARKKRRYEKV MKKARKKAQS IADSEEMNEA SKSKTINSLI KKAQKISSKK RVNVYTVTRR HGLSKQIKNK EKSINTTNLK TKFVDKRLKK DKRAIKHINK KMKHKSKQRN KKR

NT Sequence

atgaatgata atattcaaga atcttttaat caaaatggta gtgatgatga agatatggta gagcaaatga gttttaactc tgagaattct gatactgatg aagtccaaga aagtgatgat gagaggttta caaaacctga aaatgaggaa gatcttaaga taattcaggg aattggatca ttattagtta acaaatcaac tagaatggac ttaatcgatg gtagttataa tagatatgca tttcatgatc cagatgagga tcttgaaaac aatgttctag gacttccttc ttggtttatt gaggatgaaa ataaacataa caagccagag cttccaatta caaaagaatt gatggctcaa tatagagcaa aattaaggga aataaaaaat agaccaataa gaaaagaaag tgaagcttta gcaagaaaga agaggaggta tgagaaggtt atgaagaagg ctagaaagaa agcacaatcc attgctgatt ctgaagagat gaatgaagct tccaaatcca aaaccattaa tagcttgatt aaaaaagctc aaaagatttc tagtaagaaa agagttaatg tatatactgt aacaagaaga catgggttaa gcaaacaaat aaagaataaa gaaaaaagta ttaatactac gaatcttaag acaaaattcg tagataaaag actaaagaaa gataagagag ccattaaaca tatcaataag aagatgaaac ataagagcaa acaacgtaac aagaagaggt aa

Details for CrhoA.18340.a.B1.GE35458

HARVESTED ON:	6/26/2012
SEQUENCED ON:	6/29/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	80

Validated AA Sequence

MAHHHHHHMN DNIQESFNQN GSDDEDMVEQ MSFNSENSDT DEVQESDDER FTKPENEEDL KIIQGIGSLL VNKSTRMDLI DGSYNRYAFH DPDEDLENNV LGLPSWFIED ENKHNKPELP ITKELMAQYR AKLREIKNRP IRKESEALAR KKRRYEKVMK KARKKAQSIA DSEEMNEASK SKTINSLIKK AQKISSKKRV NVYTVTRKVS E

Validated NT Sequence

atcttactca cttacctttc ttgttacagt atatacatta actcttttct tactagaaat cttttgagct tttttaatca agctattaat ggttttggat ttggaagctt cattcatctc ttcagaatca gcaatggatt gtgctttctt tctagccttc ttcataacct tctcatacct cctcttcttt cttgctaaag cttcactttc ttttcttatt ggtctatttt ttatttccct taattttgct ctatattgag ccatcaattc ttttgtaatt ggaagctctg gcttgttatg tttattttca tcctcaataa accaagaagg aagtcctaga acattgtttt caagatcctc atctggatca tgaaatgcat atctattata actaccatcg attaagtcca ttctagttga tttgttaact aataatgatc caattccctg aattatctta agatcttcct cattttcagg ttttgtaaac ctctcatcat cactttcttg gacttcatca gtatcagaat tctcagagtt aaaactcatt tgctctacca tatcttcatc atcactacca ttttgattaa aagattcttg aatattatca ttcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg ataatattca agaatctttt aatcaaaatg gtagtgatga tgaagatatg gtagagcaaa tgagttttaa ctctgagaat tctgatactg atgaagtcca agaaagtgat gatgagaggt ttacaaaacc tgaaaatgag gaagatctta agataattca gggaattgga tcattattag ttaacaaatc aactagaatg gacttaatcg atggtagtta taatagatat gcatttcatg atccagatga ggatcttgaa aacaatgttc taggacttcc ttcttggttt attgaggatg aaaataaaca taacaagcca gagcttccaa ttacaaaaga attgatggct caatatagag caaaattaag ggaaataaaa aatagaccaa taagaaaaga aagtgaagct ttagcaagaa agaagaggag gtatgagaag gttatgaaga aggctagaaa gaaagcacaa tccattgctg attctgaaga gatgaatgaa gcttccaaat ccaaaaccat taatagcttg attaaaaaag ctcaaaagat ttctagtaag aaaagagtta atgtatatac tgtaacaaga agacatgggt taagcaaaca aataaagaat aaagaaaaaa gtattaatac tacgaatctt aagacaaaat tcgtagataa aagactaaag aaagataaga gagccattaa acatatcaat aagaagatga aacataagag caaacaacgt aacaagaaga ggtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat