CrhoA.18283.a

AMP deaminase, putative

CENTER ID: CrhoA.18283.a
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.18283.a.B1.GE35454 full length 1 438
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.40214
RefSeq: XP_667873.1
UniProt: Q5CLZ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDAIRNCFQR FDRFNEKYNP FGSNLMRDIF LKYNNPIKGK YLAEITKEVI QDLKTTHYQF VEWRISVYGK DKSEWKTLAE WLYNNGLYCK HVRWIIQIPR LYNIFYKDGC VKTFSEMLEN IFSPLIEALI NPKDNPLIFI LLTNIVGWDT VDDESQISKY SMDNPNFCYP EYWRSGDNPP YSYWGFYLYS NIRVLNQLLY SRGLNPLMFR PHCGEAGKIS HLATMYLLAD SINHGILLKK TPVLQYLYYL KQIGIAVSPV SNNALFLELM KNPFPKFFNV GLNVSLSTDD PLIFHFTDES LLEEYSIASH IWKLNNIDLC EIARNSVLQS GFSPKYKASW LGVKNYSHLN KSLYNILNDL EPCEINDISR SNVPNIRIQF RKDMLKGEMD LINKYTVSID RIENAISIVK NLRLKNVSDN ISKEGVNRNS NSLMNNFP
NT Sequence
atggatgcaa ttaggaactg ttttcaaagg tttgatagat ttaacgagaa atataatcca ttcggttcta acctgatgag agatatattc ttgaaatata acaatccaat taagggaaaa tatcttgctg aaattacaaa ggaagtaatt caagatttaa aaactacaca ttatcaattt gtggaatgga gaatcagtgt ctacggtaaa gacaagagcg aatggaaaac tttagcagag tggctatata ataatggttt atattgtaaa catgtaagat ggatcattca gattccaaga ctttataata ttttttacaa agatgggtgc gttaaaacat tcagtgaaat gctcgaaaat atattttccc cattaataga agcattgatc aatccaaaag ataatcccct gatttttatt ttacttacta atattgttgg ttgggacaca gtagatgatg aaagccaaat aagtaagtat tcaatggata acccgaactt ttgttatcca gaatattgga gatcaggaga taacccacca tactcttatt ggggcttcta tctttatagt aatattcgag tgctaaatca acttttatac tctagaggtt taaatccatt gatgttcagg cctcattgtg gagaagctgg aaaaatctca cacttagcta caatgtattt attagctgat tctattaacc acggaatatt acttaaaaaa actccagtat tgcaatattt atactacttg aaacaaattg gcattgcagt ttctcctgtt tctaacaatg cattgttttt ggagttaatg aaaaatccat tcccaaagtt ttttaatgtt ggattaaatg tctcgctatc aactgatgat ccccttattt ttcactttac agatgaatct cttctcgaag agtattctat tgcctctcat atttggaaat tgaacaatat tgacctttgc gagatcgcga gaaattcggt tcttcagtct ggattttcac caaaatataa ggcaagttgg ttaggtgtga aaaactacag tcatttaaat aaatcactat ataatatttt aaatgatttg gaaccttgtg aaatcaacga tatttcgagg tcgaatgtcc caaacattag aatccagttt agaaaagata tgcttaaagg tgaaatggac ctcataaata agtatacagt ttcaattgat agaatcgaaa atgcaataag tattgtaaaa aatttaagat tgaaaaatgt ttccgataat ataagcaaag aaggagttaa tcgtaactct aattccttaa tgaataattt cccctaa
Details for CrhoA.18283.a.B1.GE35454
HARVESTED ON: data unavailable
SEQUENCED ON: 6/29/2012
EXPECTED MW: 52kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 96
PERCENT COVERAGE: 72
Validated AA Sequence
MAHHHHHMDA IRNCFQRFDR FNEKYNPFGS NLMRDIFLKY NNPIKGKYLA EITKEVIQDL KTTHYQFVEW RISVYGKDKS EWKTLAEWLY NNGLYCKHVR WIIQIPRLYN IFYKDGCVKT FSEMLENIFS PLIEALINPK DNPLILILLT NIVGWDTVDD ESQISKYSMD NPNFCYPEYW RSGDNPPYSY WGFYLYSNIR VLNQLLYSRG LNPLMFRPHC GEAGKISHLA TMYLLADSIN HGILLKKTPV LQYLYYLKQI GIAVSPVSNN ALFLELMKNP FPKFFNVGXN VSLSTDDPLI FHFTDESLLX XXSIASHIXX XXX
Validated NT Sequence
attttgttnn ntttaagaag gaganatacc atggctcacc accaccacca tatggatgca attaggaact gttttcaaag gtttgataga tttaacgaga aatataatcc attcggttct aacctgatga gagatatatt cttgaaatat aacaatccaa ttaagggaaa atatcttgct gaaattacaa aggaagtaat tcaagattta aaaactacac attatcaatt tgtggaatgg agaatcagtg tctacggtaa agacaagagc gaatggaaaa ctttagcaga gtggctatat aataatggtt tatattgtaa acatgtaaga tggatcattc agattccaag actttataat attttttaca aagatgggtg cgttaaaaca ttcagtgaaa tgctcgaaaa tatattttcc ccattaatag aagcattgat caatccaaaa gataatcccc tgattcttat tttacttact aatattgttg gttgggacac agtagatgat gaaagccaaa taagtaagta ttcaatggat aacccgaact tttgttatcc agaatattgg agatcaggag ataacccacc atactcttat tggggcttct atctttatag taatattcga gtgctaaatc aacttttata ctctagaggt ttaaatccat tgatgttcag gcctcattgt ggagaagctg gaaaaatctc acacttagct acaatgtatt tattagctga ttctattaac cacggaatat tacttaaaaa aactccagta ttgcaatatt tatactactt gaaacaaatt ggcattgcag tttctcctgt ttctaacaat gcattgtttt tggagttaat gaaaaatcca ttcccnaagt tttttaatgt tggantaaat gtctcgctat caactgatga tccccttatt tttcacttta cagatgaatc tcttctcgan nagtantcta ttgcctctca tatttngnan nnganannnt ganctttgcn agatcgcgag aaantcgtct nagtctggat ttcncnnnan nnnnagtnnn ngnngnnaaa actncngtcn ttnnntaann nctnnnnann ttnnnngant nnnnnnnnna ntnannnttn nnnnncnann nnnnnnnnna nncnnnnnaa nnnngnntnn nnnnnnnnnn nnnnnnnnnn nnnnancnna nnnnnnnnnn nnnnnnnann nnnnaa
Expected Protein Sequence
MAHHHHHHMD AIRNCFQRFD RFNEKYNPFG SNLMRDIFLK YNNPIKGKYL AEITKEVIQD LKTTHYQFVE WRISVYGKDK SEWKTLAEWL YNNGLYCKHV RWIIQIPRLY NIFYKDGCVK TFSEMLENIF SPLIEALINP KDNPLIFILL TNIVGWDTVD DESQISKYSM DNPNFCYPEY WRSGDNPPYS YWGFYLYSNI RVLNQLLYSR GLNPLMFRPH CGEAGKISHL ATMYLLADSI NHGILLKKTP VLQYLYYLKQ IGIAVSPVSN NALFLELMKN PFPKFFNVGL NVSLSTDDPL IFHFTDESLL EEYSIASHIW KLNNIDLCEI ARNSVLQSGF SPKYKASWLG VKNYSHLNKS LYNILNDLEP CEINDISRSN VPNIRIQFRK DMLKGEMDLI NKYTVSIDRI ENAISIVKNL RLKNVSDNIS KEGVNRNSNS LMNNFP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatg caattaggaa ctgttttcaa aggtttgata gatttaacga gaaatataat ccattcggtt ctaacctgat gagagatata ttcttgaaat ataacaatcc aattaaggga aaatatcttg ctgaaattac aaaggaagta attcaagatt taaaaactac acattatcaa tttgtggaat ggagaatcag tgtctacggt aaagacaaga gcgaatggaa aactttagca gagtggctat ataataatgg tttatattgt aaacatgtaa gatggatcat tcagattcca agactttata atatttttta caaagatggg tgcgttaaaa cattcagtga aatgctcgaa aatatatttt ccccattaat agaagcattg atcaatccaa aagataatcc cctgattttt attttactta ctaatattgt tggttgggac acagtagatg atgaaagcca aataagtaag tattcaatgg ataacccgaa cttttgttat ccagaatatt ggagatcagg agataaccca ccatactctt attggggctt ctatctttat agtaatattc gagtgctaaa tcaactttta tactctagag gtttaaatcc attgatgttc aggcctcatt gtggagaagc tggaaaaatc tcacacttag ctacaatgta tttattagct gattctatta accacggaat attacttaaa aaaactccag tattgcaata tttatactac ttgaaacaaa ttggcattgc agtttctcct gtttctaaca atgcattgtt tttggagtta atgaaaaatc cattcccaaa gttttttaat gttggattaa atgtctcgct atcaactgat gatcccctta tttttcactt tacagatgaa tctcttctcg aagagtattc tattgcctct catatttgga aattgaacaa tattgacctt tgcgagatcg cgagaaattc ggttcttcag tctggatttt caccaaaata taaggcaagt tggttaggtg tgaaaaacta cagtcattta aataaatcac tatataatat tttaaatgat ttggaacctt gtgaaatcaa cgatatttcg aggtcgaatg tcccaaacat tagaatccag tttagaaaag atatgcttaa aggtgaaatg gacctcataa ataagtatac agtttcaatt gatagaatcg aaaatgcaat aagtattgta aaaaatttaa gattgaaaaa tgtttccgat aatataagca aagaaggagt taatcgtaac tctaattcct taatgaataa tttcccctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at