CrhoA.18226.a

DNA-directed RNA polymerases I and III subunit RPAC1, putative

CENTER ID: CrhoA.18226.a
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.18226.a.B1.GE35502 full length 1 344
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.80042
RefSeq: XP_667165.1
UniProt: Q5CJZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVSEKKERNN RYLECTTEGP RNVGGYGYIG SNELNSIIKG DNYRKQTVEE IIETIDIKVN ELKSDYINFD ISGIDASIAN ALRRILLVEI PTVAIETVQI YQNNGVIQDE VLAHRLGLIP MLIDPNTIEF RNEDEDLNEK NSVCFKLNVK CTKDDINHEK GITSKAIYSK DLVWIPLSDN QRKKFGKSPP KVVTDDILIT KLRPGQSIEA ILYCEKNIGR VHAKWSPVCT ASYRLLPEFG FPNGPISGSD ALKLRDTVCP MKVFDIEDVT GNAVPTNPRN CTTCRACIEK FPNKIKLLKD KFHFIFSIET TGCIPPVELV KTAIDILRKK GEKIKSSVKL FYEQ
NT Sequence
atggtttcag agaagaaaga aaggaataat agatatttag aatgtacgac agaaggcccg agaaatgtag gaggatatgg ttacatagga agcaatgagt taaattcaat tattaaggga gataattata gaaaacaaac ggttgaggaa ataatagaaa cgatcgatat aaaagtgaac gagcttaaga gtgattatat taactttgac atttctggaa tagatgcatc catagcaaac gctcttagaa ggattttatt ggtagaaatt ccaacagtag cgatcgaaac ggtccaaatt taccagaata atggagtaat tcaggatgag gttttagctc acagactcgg actaatccca atgctaatcg atccaaatac aatcgaattt agaaatgaag atgaagacct caatgaaaag aatagtgttt gcttcaaact gaatgtgaaa tgtactaaag atgatataaa tcacgaaaaa ggaatcacaa gtaaagcaat ttactcgaaa gatttggtat ggattccact tagtgataat caaaggaaaa agtttggaaa gagtcccccg aaagttgtta ctgatgatat attaattaca aaacttagac ctggccaatc aatagaggca attctttact gtgaaaaaaa tattgggaga gttcatgcaa aatggagtcc cgtttgtact gcaagttacc gtcttttacc tgaatttggt ttcccaaatg gccctatttc tggctcggat gcacttaagc taagagatac agtatgtcct atgaaagttt ttgatattga agatgttact gggaatgctg tccctactaa cccaagaaac tgtaccacct gtagagcatg tattgaaaaa tttccaaata agataaagct cttaaaggac aagtttcact ttattttttc gattgaaacc acaggatgta ttcctcctgt tgaactggtt aaaactgcca tagatatttt gcgaaagaag ggtgagaaga ttaagagttc cgtcaagttg ttttatgagc agtga
Details for CrhoA.18226.a.B1.GE35502
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMV SEKKERNNRY LECTTEGPRN VGGYGYIGSN ELNSIIKGDN YRKQTVEEII ETIDIKVNEL KSDYINFDIS GIDASIANAL RRILLVEIPT VAIETVQIYQ NNGVIQDEVL AHRLGLIPML IDPNTIEFRN EDEDLNEKNS VCFKLNVKCT KDDINHEKGI TSKAIYSKDL VWIPLSDNQR KKFGKSPPKV VTDDILITKL RPGQSIEAIL YCEKNIGRVH AKWSPVCTAS YRLLPEFGFP NGPISGSDAL KLRDTVCPMK VFDIEDXTGX AVPTNPRNCT XCRACXENXQ IXKAXXXXXT LFFXXXXXXS XXXXXXXXFX XXXXXXXQXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttttgtttac tttaagaagg aganatacca tggctcacca ccaccaccac catatggttt cagagaagaa agaaaggaat aatagatatt tagaatgtac gacagaaggc ccgagaaatg taggaggata tggttacata ggaagcaatg agttaaattc aattattaag ggagataatt atagaaaaca aacggttgag gaaataatag aaacgatcga tataaaagtg aacgagctta agagtgatta tattaacttt gacatttctg gaatagatgc atccatagca aacgctctta gaaggatttt attggtagaa attccaacag tagcgatcga aacggtccaa atttaccaga ataatggagt aattcaggat gaggttttag ctcacagact cggactaatc ccaatgctaa tcgatccaaa tacaatcgaa tttagaaatg aagatgaaga cctcaatgaa aagaatagtg tttgcttcaa actgaatgtg aaatgtacta aagatgatat aaatcacgaa aaaggaatca caagtaaagc aatttactcg aaagatttgg tatggattcc acttagtgat aatcaaagga aaaagtttgg aaagagtccc ccgaaagttg ttactgatga tatattaatt acaaaactta gacctggcca atcaatagag gcaattcttt actgtgaaaa aaatattggg agagttcatg caaaatggag tcccgtttgt actgcaagtt accgtctttt acctgaattt ggtttcccaa atggccctat ttctggctcg gatgcactta agctaagaga tacagtatgt cctatgaaag tttttgatat tgaagatgnt actgggnatg ctgtccctac taacccnaga aactgtacca nctgtagagc atgtanngaa aatttncaaa tannnaaagc nntnanngan nntttnacnt tatttttcna nngnancncn nntgnntcnc nntnannnnn anntncnnnn nnntttncna nnnnnnnnan annnnntnnn cagtnntnnn nnngnannnn nannnncnnn nnnnnnnnnn nnnnnnngnn nnnnnnnnnn nnnnannann nnnt
Expected Protein Sequence
MAHHHHHHMV SEKKERNNRY LECTTEGPRN VGGYGYIGSN ELNSIIKGDN YRKQTVEEII ETIDIKVNEL KSDYINFDIS GIDASIANAL RRILLVEIPT VAIETVQIYQ NNGVIQDEVL AHRLGLIPML IDPNTIEFRN EDEDLNEKNS VCFKLNVKCT KDDINHEKGI TSKAIYSKDL VWIPLSDNQR KKFGKSPPKV VTDDILITKL RPGQSIEAIL YCEKNIGRVH AKWSPVCTAS YRLLPEFGFP NGPISGSDAL KLRDTVCPMK VFDIEDVTGN AVPTNPRNCT TCRACIEKFP NKIKLLKDKF HFIFSIETTG CIPPVELVKT AIDILRKKGE KIKSSVKLFY EQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttt cagagaagaa agaaaggaat aatagatatt tagaatgtac gacagaaggc ccgagaaatg taggaggata tggttacata ggaagcaatg agttaaattc aattattaag ggagataatt atagaaaaca aacggttgag gaaataatag aaacgatcga tataaaagtg aacgagctta agagtgatta tattaacttt gacatttctg gaatagatgc atccatagca aacgctctta gaaggatttt attggtagaa attccaacag tagcgatcga aacggtccaa atttaccaga ataatggagt aattcaggat gaggttttag ctcacagact cggactaatc ccaatgctaa tcgatccaaa tacaatcgaa tttagaaatg aagatgaaga cctcaatgaa aagaatagtg tttgcttcaa actgaatgtg aaatgtacta aagatgatat aaatcacgaa aaaggaatca caagtaaagc aatttactcg aaagatttgg tatggattcc acttagtgat aatcaaagga aaaagtttgg aaagagtccc ccgaaagttg ttactgatga tatattaatt acaaaactta gacctggcca atcaatagag gcaattcttt actgtgaaaa aaatattggg agagttcatg caaaatggag tcccgtttgt actgcaagtt accgtctttt acctgaattt ggtttcccaa atggccctat ttctggctcg gatgcactta agctaagaga tacagtatgt cctatgaaag tttttgatat tgaagatgtt actgggaatg ctgtccctac taacccaaga aactgtacca cctgtagagc atgtattgaa aaatttccaa ataagataaa gctcttaaag gacaagtttc actttatttt ttcgattgaa accacaggat gtattcctcc tgttgaactg gttaaaactg ccatagatat tttgcgaaag aagggtgaga agattaagag ttccgtcaag ttgttttatg agcagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat