SSGCID: Target Page

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
CrhoA.18219.a.B1.GE35501	full length	1	466
CrhoA.18219.a.B6.GE42434	kphk-end	40	466
CrhoA.18219.a.SA1.GE42275	full length	1	466
CrhoA.18219.a.SA2.GE42276	Construct 2	39	466
CrhoA.18219.a.SA3.GE42277	Construct 3	26	466
CrhoA.18219.a.SA4.GE42278	Construct 4	32	466
CrhoA.18219.a.SA5.GE42279	Construct 5	38	466

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.18219.a.B1.PS01624	full length		1	466
CrhoA.18219.a.B1.PW38435	full length		1	466

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:Chro.30047
OrthoMCL:	OG5_127597
RefSeq:	XP_667560.1
UniProt:	Q5CL53

Details for CrhoA.18219.a.B1.GE35501

HARVESTED ON:	6/26/2012
SEQUENCED ON:	6/29/2012
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	91

Validated AA Sequence

MSLGSFMNTA ANAIKPHKFW NTQPVVQNDD SSTEYSFGPI EIEPDSFRKE IYKLPDGFSW FDCNLWDIES QDFEDTYQLL KDHYVEDDDS QFRFNYSKEF LRWALCVPGQ KKNWLVGVRV NETKKMVGFI SAIPIKVRIH NCIMNTSVVN FLCVHKKLRS KRLAPVLIKE ITRRIRCEKI FQSIYTCGKN ITKPFTIGTY WHRIINVKKL LETGFIGIPR NMTMSSLIKY HRIPADKRIE GFRPSVDSDA EQICKLFENY FMKYKDISNE TMNNLINYDE INHSKELGKQ AYMKLDKIED LQDKIIIHQC FNVEDVKHYF TNIDKVIVTY VRENKNKEIT DLFSFFIIES TVINNERFPT INIAYSYFNI ANTCSLKELF NEMIITAKNN NCDAFNTLDL MQNLQVIQDN KFIIGTGKLR YYVFNWKIPQ IS

Validated NT Sequence

aggtgaaatt tgaggtatct tccaattaaa cacataatat cttaatttac cggtaccgat aataaattta ttatcttgaa taacttgtaa attttgcatc agatccaatg tattaaaagc atcacaatta ttatttttgg cagtaataat catttcattg aacaattctt ttaatgagca ggtatttgca atattaaagt atgaataagc aatattaata gttggaaatc tttcattatt gatgacagtg gattctatga taaagaaact aaacaagtca gtaatttcct tatttttatt ttctcgaaca taagttacaa taactttatc aatattcgtg aaataatgtt taacatcttc aacattaaag cattgatgaa ttatgatttt gtcttgcaaa tcttcaatct tatccagctt catatatgct tgttttccaa gctcttttga gtggttaatt tcatcatagt ttatcagatt attcatggtt tcgttgctga tatctttata tttcataaag taattttcga ataatttaca tatttgttct gcatcagaat ctacggaggg tctaaaccct tcaatccttt tatcagcagg aatacgatga tatttgatta aagagctcat ggtcatattt cttgggattc caataaaacc agtttctaat aatttcttta cattaattat acgatgccaa taagttccaa tagtaaaagg tttggtaata ttcttcccgc aagtgtaaat tgattgaaag attttttcac atctaattct tctcgtaatt tccttaataa gtacaggtgc aagtctttta gatctaagct tcttatgaac acagagaaaa ttaactacag aagtattcat aatgcaatta tgaattctaa ctttgattgg gattgctgaa ataaatccaa ccattttctt tgtttcatta actctgactc caacaagcca gttttttttt tgaccaggga cacaaagagc ccatctaaga aactcctttg aataattaaa tctaaattgg gaatcgtcat cttcaacata atggtctttt aataattggt aagtatcttc aaaatcttga gattctatat cccataaatt acaatcaaac caagagaatc catctggtag cttatatatt tcttttctaa aagaatctgg ttcaatttca attggtccaa atgaatattc agtggaagaa tcatcgtttt gaactactgg ttgagtattc cagaatttgt gtggtttgat tgcatttgca gcagtgttca taaagctacc taagctcatt tctttcagaa gatttgtgat cttcttggta tttgaaatgg aagattctga tttttctggg gtttc

Expected Protein Sequence

MAHHHHHHMS GETPEKSESS ISNTKKITNL LKEMSLGSFM NTAANAIKPH KFWNTQPVVQ NDDSSTEYSF GPIEIEPDSF RKEIYKLPDG FSWFDCNLWD IESQDFEDTY QLLKDHYVED DDSQFRFNYS KEFLRWALCV PGQKKNWLVG VRVNETKKMV GFISAIPIKV RIHNCIMNTS VVNFLCVHKK LRSKRLAPVL IKEITRRIRC EKIFQSIYTC GKNITKPFTI GTYWHRIINV KKLLETGFIG IPRNMTMSSL IKYHRIPADK RIEGFRPSVD SDAEQICKLF ENYFMKYKDI SNETMNNKIN YDEINHSKEL GKQAYMKLDK IEDLQDKIII HQCFNVEDVK HYFTNIDKVI VTYVRENKNK EITDLFSFFI IESTVINNER FPTINIAYSY FNIANTCSLK ELFNEMIITA KNNNCDAFNT LDLMQNLQVI QDNKFIIGTG KLRYYVFNWK IPQISPSNVG IILF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg gtgaaacccc agaaaaatca gaatcttcca tttcaaatac caagaagatc acaaatcttc tgaaagaaat gagcttaggt agctttatga acactgctgc aaatgcaatc aaaccacaca aattctggaa tactcaacca gtagttcaaa acgatgattc ttccactgaa tattcatttg gaccaattga aattgaacca gattctttta gaaaagaaat atataagcta ccagatggat tctcttggtt tgattgtaat ttatgggata tagaatctca agattttgaa gatacttacc aattattaaa agaccattat gttgaagatg acgattccca atttagattt aattattcaa aggagtttct tagatgggct ctttgtgtcc ctggtcaaaa aaaaaactgg cttgttggag tcagagttaa tgaaacaaag aaaatggttg gatttatttc agcaatccca atcaaagtta gaattcataa ttgcattatg aatacttctg tagttaattt tctctgtgtt cataagaagc ttagatctaa aagacttgca cctgtactta ttaaggaaat tacgagaaga attagatgtg aaaaaatctt tcaatcaatt tacacttgcg ggaagaatat taccaaacct tttactattg gaacttattg gcatcgtata attaatgtaa agaaattatt agaaactggt tttattggaa tcccaagaaa tatgaccatg agctctttaa tcaaatatca tcgtattcct gctgataaaa ggattgaagg gtttagaccc tccgtagatt ctgatgcaga acaaatatgt aaattattcg aaaattactt tatgaaatat aaagatatca gcaacgaaac catgaataat aaaataaact atgatgaaat taaccactca aaagagcttg gaaaacaagc atatatgaag ctggataaga ttgaagattt gcaagacaaa atcataattc atcaatgctt taatgttgaa gatgttaaac attatttcac gaatattgat aaagttattg taacttatgt tcgagaaaat aaaaataagg aaattactga cttgtttagt ttctttatca tagaatccac tgtcatcaat aatgaaagat ttccaactat taatattgct tattcatact ttaatattgc aaatacctgc tcattaaaag aattgttcaa tgaaatgatt attactgcca aaaataataa ttgtgatgct tttaatacat tggatctgat gcaaaattta caagttattc aagataataa atttattatc ggtaccggta aattaagata ttatgtgttt aattggaaga tacctcaaat ttcaccttca aatgttggaa ttatattatt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for CrhoA.18219.a.B6.GE42434

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	94
PERCENT COVERAGE:	26

Validated AA Sequence

MAHHHHHHMK PHKFWNTQPV VQNDDSSTEY SFGPIEIEPD SFRKEIYKLP DGFSWFDCNL WDIESQDFED TYQLLKDHYV EDDDSQFRFN YSKEFLRWAL CVPGQKKKLA CWSQS

Validated NT Sequence

gaaggtgaaa cttgaggtat cttccaatta aacacataat atcttaattt accggtaccg ataataaatt tattatcttg aataacttgt aaattttgca tcagatccaa tgtattaaaa gcatcacaat tattattttt ggcagtaata atcatttcat tgaacaattc ttttaatgag caggtatttg caatattaaa gtatgaataa gcaatattaa tagttggaaa tctttcatta ttgatgacag tggattctat gataaagaaa ctaaacaagt cagtaatttc cttattttta ttttctcgaa cataagttac aataacttta tcaatattcg tgaaataatg tttaacatct tcaacattaa agcattgatg aattatgatt ttgtcttgca aatcttcaat cttatccagc ttcatatatg cttgttttcc aagctctttt gagtggttaa tttcatcata gtttatcaga ttattcatgg tttcgttgct gatatcttta tatttcataa agtaattttc gaataattta catatttgtt ctgcatcaga atctacggag ggtctaaacc cttcaatcct tttatcagca ggaatacgat gatatttgat taaagagctc atggtcatat ttcttgggat tccaataaaa ccagtttcta ataatttctt tacattaatt atacgatgcc aataagttcc aatagtaaaa ggtttggtaa tattcttccc gcaagtgtaa attgattgaa agattttttc acatctaatt cttctcgtaa tttccttaat aagtacaggt gcaagtcttt tagatctaag cttcttatga acacagggaa aattaactac agaagtattc ataatgcaat tatgaattct aactttgatt gggattgctg aaataaatcc aaccattttc tttgtttcat taactctgac tccagcaagc cagttttttt ttttgaccag ggacacaaag agcccatcta agaaactcct ttgaataatt aaatctaaat tgggaatcgt catcttcaac ataatggtct tttaataatt ggtaagtatc ttcaaaatct tgagattcta tatcccataa attacaatca aaccaagaga atccatctgg tagcttatat atttcttttc taaaagaatc tggttcaatt tcaattggtc caaatgaata ttcagtggaa gaatcatcgt tttgaactac tggttgagta ttccagaatt tgtgtggttt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHKP HKFWNTQPVV QNDDSSTEYS FGPIEIEPDS FRKEIYKLPD GFSWFDCNLW DIESQDFEDT YQLLKDHYVE DDDSQFRFNY SKEFLRWALC VPGQKKNWLV GVRVNETKKM VGFISAIPIK VRIHNCIMNT SVVNFLCVHK KLRSKRLAPV LIKEITRRIR CEKIFQSIYT CGKNITKPFT IGTYWHRIIN VKKLLETGFI GIPRNMTMSS LIKYHRIPAD KRIEGFRPSV DSDAEQICKL FENYFMKYKD ISNETMNNKI NYDEINHSKE LGKQAYMKLD KIEDLQDKII IHQCFNVEDV KHYFTNIDKV IVTYVRENKN KEITDLFSFF IIESTVINNE RFPTINIAYS YFNIANTCSL KELFNEMIIT AKNNNCDAFN TLDLMQNLQV IQDNKFIIGT GKLRYYVFNW KIPQISPSNV GIILF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaaccac acaaattctg gaatactcaa ccagtagttc aaaacgatga ttcttccact gaatattcat ttggaccaat tgaaattgaa ccagattctt ttagaaaaga aatatataag ctaccagatg gattctcttg gtttgattgt aatttatggg atatagaatc tcaagatttt gaagatactt accaattatt aaaagaccat tatgttgaag atgacgattc ccaatttaga tttaattatt caaaggagtt tcttagatgg gctctttgtg tccctggtca aaaaaaaaac tggcttgttg gagtcagagt taatgaaaca aagaaaatgg ttggatttat ttcagcaatc ccaatcaaag ttagaattca taattgcatt atgaatactt ctgtagttaa ttttctctgt gttcataaga agcttagatc taaaagactt gcacctgtac ttattaagga aattacgaga agaattagat gtgaaaaaat ctttcaatca atttacactt gcgggaagaa tattaccaaa ccttttacta ttggaactta ttggcatcgt ataattaatg taaagaaatt attagaaact ggttttattg gaatcccaag aaatatgacc atgagctctt taatcaaata tcatcgtatt cctgctgata aaaggattga agggtttaga ccctccgtag attctgatgc agaacaaata tgtaaattat tcgaaaatta ctttatgaaa tataaagata tcagcaacga aaccatgaat aataaaataa actatgatga aattaaccac tcaaaagagc ttggaaaaca agcatatatg aagctggata agattgaaga tttgcaagac aaaatcataa ttcatcaatg ctttaatgtt gaagatgtta aacattattt cacgaatatt gataaagtta ttgtaactta tgttcgagaa aataaaaata aggaaattac tgacttgttt agtttcttta tcatagaatc cactgtcatc aataatgaaa gatttccaac tattaatatt gcttattcat actttaatat tgcaaatacc tgctcattaa aagaattgtt caatgaaatg attattactg ccaaaaataa taattgtgat gcttttaata cattggatct gatgcaaaat ttacaagtta ttcaagataa taaatttatt atcggtaccg gtaaattaag atattatgtg tttaattgga agatacctca aatttcacct tcaaatgttg gaattatatt attttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for CrhoA.18219.a.SA1.GE42275

HARVESTED ON:	3/23/2018
SEQUENCED ON:	2/28/2018
EXPECTED MW:	57kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSGETPEKS ESSISNTKKI TNLLKEMSLG SFMNTAANAI KPHKFWNTQP VVQNDDSSTE YSFGPIEIEP DSFRKEIYKL PDGFSWFDCN LWDIESQDFE DTYQLLKDHY VEDDDSQFRF NYSKEFLRWA LCVPGQKKNW LVGVRVNETK KMVGFISAIP IKVRIHNCIM NTSVVNFLCV HKKLRSKRLA PVLIKEITRR IRCEKIFQSI YTCGKNITKP FTIGTYWHRI INVKKLLETG FIGIPRNMTM SSLIKYHRIP ADKRIEGFRP SVDSDAEQIC KLFENYFMKY KDISNETMNN KINYDEINHS KELGKQAYMK LDKIEDLQDK IIIHQCFNVE DVKHYFTNID KVIVTYVREN KNKEITDLFS FFIIESTVIN NERFPTINIA YSYFNIANTC SLKELFNEMI ITAKNNNCDA FNTLDLMQNL QVIQDNKFII GTGKLRYYVF NWKIPQISPS NVGIILF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggatccat gagtggtgaa accccagaaa aatcagaatc ttccatttca aataccaaga agatcacaaa tcttctgaaa gaaatgagct taggtagctt tatgaacact gctgcaaatg caatcaaacc acacaaattc tggaatactc aaccagtagt tcaaaacgat gattcttcca ctgaatattc atttggacca attgaaattg aaccagattc ttttagaaaa gaaatatata agctaccaga tggattctct tggtttgatt gtaatttatg ggatatagaa tctcaagatt ttgaagatac ttaccaatta ttaaaagacc attatgttga agatgacgat tcccaattta gatttaatta ttcaaaggag tttcttagat gggctctttg tgtccctggt caaaaaaaaa actggcttgt tggagtcaga gttaatgaaa caaagaaaat ggttggattt atttcagcaa tcccaatcaa agttagaatt cataattgca ttatgaatac ttctgtagtt aattttctct gtgttcataa gaagcttaga tctaaaagac ttgcacctgt acttattaag gaaattacga gaagaattag atgtgaaaaa atctttcaat caatttacac ttgcgggaag aatattacca aaccttttac tattggaact tattggcatc gtataattaa tgtaaagaaa ttattagaaa ctggttttat tggaatccca agaaatatga ccatgagctc tttaatcaaa tatcatcgta ttcctgctga taaaaggatt gaagggttta gaccctccgt agattctgat gcagaacaaa tatgtaaatt attcgaaaat tactttatga aatataaaga tatcagcaac gaaaccatga ataataaaat aaactatgat gaaattaacc actcaaaaga gcttggaaaa caagcatata tgaagctgga taagattgaa gatttgcaag acaaaatcat aattcatcaa tgctttaatg ttgaagatgt taaacattat ttcacgaata ttgataaagt tattgtaact tatgttcgag aaaataaaaa taaggaaatt actgacttgt ttagtttctt tatcatagaa tccactgtca tcaataatga aagatttcca actattaata ttgcttattc atactttaat attgcaaata cctgctcatt aaaagaattg ttcaatgaaa tgattattac tgccaaaaat aataattgtg atgcttttaa tacattggat ctgatgcaaa atttacaagt tattcaagat aataaattta ttatcggtac cggtaaatta agatattatg tgtttaattg gaagatacct caaatttcac cttcaaatgt tggaattata ttattttaac tcgaggctcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for CrhoA.18219.a.SA2.GE42276

HARVESTED ON:	3/23/2018
SEQUENCED ON:	2/28/2018
EXPECTED MW:	53kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SIKPHKFWNT QPVVQNDDSS TEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE TGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDISNETM NNKINYDEIN HSKELGKQAY MKLDKIEDLQ DKIIIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MIITAKNNNC DAFNTLDLMQ NLQVIQDNKF IIGTGKLRYY VFNWKIPQIS PSNVGIILF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggatccat caaaccacac aaattctgga atactcaacc agtagttcaa aacgatgatt cttccactga atattcattt ggaccaattg aaattgaacc agattctttt agaaaagaaa tatataagct accagatgga ttctcttggt ttgattgtaa tttatgggat atagaatctc aagattttga agatacttac caattattaa aagaccatta tgttgaagat gacgattccc aatttagatt taattattca aaggagtttc ttagatgggc tctttgtgtc cctggtcaaa aaaaaaactg gcttgttgga gtcagagtta atgaaacaaa gaaaatggtt ggatttattt cagcaatccc aatcaaagtt agaattcata attgcattat gaatacttct gtagttaatt ttctctgtgt tcataagaag cttagatcta aaagacttgc acctgtactt attaaggaaa ttacgagaag aattagatgt gaaaaaatct ttcaatcaat ttacacttgc gggaagaata ttaccaaacc ttttactatt ggaacttatt ggcatcgtat aattaatgta aagaaattat tagaaactgg ttttattgga atcccaagaa atatgaccat gagctcttta atcaaatatc atcgtattcc tgctgataaa aggattgaag ggtttagacc ctccgtagat tctgatgcag aacaaatatg taaattattc gaaaattact ttatgaaata taaagatatc agcaacgaaa ccatgaataa taaaataaac tatgatgaaa ttaaccactc aaaagagctt ggaaaacaag catatatgaa gctggataag attgaagatt tgcaagacaa aatcataatt catcaatgct ttaatgttga agatgttaaa cattatttca cgaatattga taaagttatt gtaacttatg ttcgagaaaa taaaaataag gaaattactg acttgtttag tttctttatc atagaatcca ctgtcatcaa taatgaaaga tttccaacta ttaatattgc ttattcatac tttaatattg caaatacctg ctcattaaaa gaattgttca atgaaatgat tattactgcc aaaaataata attgtgatgc ttttaataca ttggatctga tgcaaaattt acaagttatt caagataata aatttattat cggtaccggt aaattaagat attatgtgtt taattggaag atacctcaaa tttcaccttc aaatgttgga attatattat tttaactcga ggctccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for CrhoA.18219.a.SA3.GE42277

HARVESTED ON:	3/23/2018
SEQUENCED ON:	2/28/2018
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSLGSFMNT AANAIKPHKF WNTQPVVQND DSSTEYSFGP IEIEPDSFRK EIYKLPDGFS WFDCNLWDIE SQDFEDTYQL LKDHYVEDDD SQFRFNYSKE FLRWALCVPG QKKNWLVGVR VNETKKMVGF ISAIPIKVRI HNCIMNTSVV NFLCVHKKLR SKRLAPVLIK EITRRIRCEK IFQSIYTCGK NITKPFTIGT YWHRIINVKK LLETGFIGIP RNMTMSSLIK YHRIPADKRI EGFRPSVDSD AEQICKLFEN YFMKYKDISN ETMNNKINYD EINHSKELGK QAYMKLDKIE DLQDKIIIHQ CFNVEDVKHY FTNIDKVIVT YVRENKNKEI TDLFSFFIIE STVINNERFP TINIAYSYFN IANTCSLKEL FNEMIITAKN NNCDAFNTLD LMQNLQVIQD NKFIIGTGKL RYYVFNWKIP QISPSNVGII LF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggatccat gagcttaggt agctttatga acactgctgc aaatgcaatc aaaccacaca aattctggaa tactcaacca gtagttcaaa acgatgattc ttccactgaa tattcatttg gaccaattga aattgaacca gattctttta gaaaagaaat atataagcta ccagatggat tctcttggtt tgattgtaat ttatgggata tagaatctca agattttgaa gatacttacc aattattaaa agaccattat gttgaagatg acgattccca atttagattt aattattcaa aggagtttct tagatgggct ctttgtgtcc ctggtcaaaa aaaaaactgg cttgttggag tcagagttaa tgaaacaaag aaaatggttg gatttatttc agcaatccca atcaaagtta gaattcataa ttgcattatg aatacttctg tagttaattt tctctgtgtt cataagaagc ttagatctaa aagacttgca cctgtactta ttaaggaaat tacgagaaga attagatgtg aaaaaatctt tcaatcaatt tacacttgcg ggaagaatat taccaaacct tttactattg gaacttattg gcatcgtata attaatgtaa agaaattatt agaaactggt tttattggaa tcccaagaaa tatgaccatg agctctttaa tcaaatatca tcgtattcct gctgataaaa ggattgaagg gtttagaccc tccgtagatt ctgatgcaga acaaatatgt aaattattcg aaaattactt tatgaaatat aaagatatca gcaacgaaac catgaataat aaaataaact atgatgaaat taaccactca aaagagcttg gaaaacaagc atatatgaag ctggataaga ttgaagattt gcaagacaaa atcataattc atcaatgctt taatgttgaa gatgttaaac attatttcac gaatattgat aaagttattg taacttatgt tcgagaaaat aaaaataagg aaattactga cttgtttagt ttctttatca tagaatccac tgtcatcaat aatgaaagat ttccaactat taatattgct tattcatact ttaatattgc aaatacctgc tcattaaaag aattgttcaa tgaaatgatt attactgcca aaaataataa ttgtgatgct tttaatacat tggatctgat gcaaaattta caagttattc aagataataa atttattatc ggtaccggta aattaagata ttatgtgttt aattggaaga tacctcaaat ttcaccttca aatgttggaa ttatattatt ttaactcgag gctccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for CrhoA.18219.a.SA4.GE42278

HARVESTED ON:	3/23/2018
SEQUENCED ON:	2/28/2018
EXPECTED MW:	53kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNTAANAIK PHKFWNTQPV VQNDDSSTEY SFGPIEIEPD SFRKEIYKLP DGFSWFDCNL WDIESQDFED TYQLLKDHYV EDDDSQFRFN YSKEFLRWAL CVPGQKKNWL VGVRVNETKK MVGFISAIPI KVRIHNCIMN TSVVNFLCVH KKLRSKRLAP VLIKEITRRI RCEKIFQSIY TCGKNITKPF TIGTYWHRII NVKKLLETGF IGIPRNMTMS SLIKYHRIPA DKRIEGFRPS VDSDAEQICK LFENYFMKYK DISNETMNNK INYDEINHSK ELGKQAYMKL DKIEDLQDKI IIHQCFNVED VKHYFTNIDK VIVTYVRENK NKEITDLFSF FIIESTVINN ERFPTINIAY SYFNIANTCS LKELFNEMII TAKNNNCDAF NTLDLMQNLQ VIQDNKFIIG TGKLRYYVFN WKIPQISPSN VGIILF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggatccat gaacactgct gcaaatgcaa tcaaaccaca caaattctgg aatactcaac cagtagttca aaacgatgat tcttccactg aatattcatt tggaccaatt gaaattgaac cagattcttt tagaaaagaa atatataagc taccagatgg attctcttgg tttgattgta atttatggga tatagaatct caagattttg aagatactta ccaattatta aaagaccatt atgttgaaga tgacgattcc caatttagat ttaattattc aaaggagttt cttagatggg ctctttgtgt ccctggtcaa aaaaaaaact ggcttgttgg agtcagagtt aatgaaacaa agaaaatggt tggatttatt tcagcaatcc caatcaaagt tagaattcat aattgcatta tgaatacttc tgtagttaat tttctctgtg ttcataagaa gcttagatct aaaagacttg cacctgtact tattaaggaa attacgagaa gaattagatg tgaaaaaatc tttcaatcaa tttacacttg cgggaagaat attaccaaac cttttactat tggaacttat tggcatcgta taattaatgt aaagaaatta ttagaaactg gttttattgg aatcccaaga aatatgacca tgagctcttt aatcaaatat catcgtattc ctgctgataa aaggattgaa gggtttagac cctccgtaga ttctgatgca gaacaaatat gtaaattatt cgaaaattac tttatgaaat ataaagatat cagcaacgaa accatgaata ataaaataaa ctatgatgaa attaaccact caaaagagct tggaaaacaa gcatatatga agctggataa gattgaagat ttgcaagaca aaatcataat tcatcaatgc tttaatgttg aagatgttaa acattatttc acgaatattg ataaagttat tgtaacttat gttcgagaaa ataaaaataa ggaaattact gacttgttta gtttctttat catagaatcc actgtcatca ataatgaaag atttccaact attaatattg cttattcata ctttaatatt gcaaatacct gctcattaaa agaattgttc aatgaaatga ttattactgc caaaaataat aattgtgatg cttttaatac attggatctg atgcaaaatt tacaagttat tcaagataat aaatttatta tcggtaccgg taaattaaga tattatgtgt ttaattggaa gatacctcaa atttcacctt caaatgttgg aattatatta ttttaactcg aggctccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for CrhoA.18219.a.SA5.GE42279

HARVESTED ON:	3/23/2018
SEQUENCED ON:	2/28/2018
EXPECTED MW:	53kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MGTLEAQTQG PGSAIKPHKF WNTQPVVQND DSSTEYSFGP IEIEPDSFRK EIYKLPDGFS WFDCNLWDIE SQDFEDTYQL LKDHYVEDDD SQFRFNYSKE FLRWALCVPG QKKNWLVGVR VNETKKMVGF ISAIPIKVRI HNCIMNTSVV NFLCVHKKLR SKRLAPVLIK EITRRIRCEK IFQSIYTCGK NITKPFTIGT YWHRIINVKK LLETGFIGIP RNMTMSSLIK YHRIPADKRI EGFRPSVDSD AEQICKLFEN YFMKYKDISN ETMNNLINYD EINHSKELGK QAYMKLDKIE DLQDKIIIHQ CFNVEDVKHY FTNIDKVIVT YVRENKNKEI TDLFSFFIIE STVINNERFP TINIAYSYFN IANTCSLKEL FNEMIITAKN NNCDAFNTLD LMQNLQVIQD NKFIIGTGKL RYYVFNWKIP Q

Validated NT Sequence

aatttgaggt atcttccaat taaacacata atatcttaat ttaccggtac cgataataaa tttattatct tgaataactt gtaaattttg catcagatcc aatgtattaa aagcatcaca attattattt ttggcagtaa taatcatttc attgaacaat tcttttaatg agcaggtatt tgcaatatta aagtatgaat aagcaatatt aatagttgga aatctttcat tattgatgac agtggattct atgataaaga aactaaacaa gtcagtaatt tccttatttt tattttctcg aacataagtt acaataactt tatcaatatt cgtgaaataa tgtttaacat cttcaacatt aaagcattga tgaattatga ttttgtcttg caaatcttca atcttatcca gcttcatata tgcttgtttt ccaagctctt ttgagtggtt aatttcatca tagtttatca gattattcat ggtttcgttg ctgatatctt tatatttcat aaagtaattt tcgaataatt tacatatttg ttctgcatca gaatctacgg agggtctaaa cccttcaatc cttttatcag caggaatacg atgatatttg attaaagagc tcatggtcat atttcttggg attccaataa aaccagtttc taataatttc tttacattaa ttatacgatg ccaataagtt ccaatagtaa aaggtttggt aatattcttc ccgcaagtgt aaattgattg aaagattttt tcacatctaa ttcttctcgt aatttcctta ataagtacag gtgcaagtct tttagatcta agcttcttat gaacacagag aaaattaact acagaagtat tcataatgca attatgaatt ctaactttga ttgggattgc tgaaataaat ccaaccattt tctttgtttc attaactctg actccaacaa gccagttttt tttttgacca gggacacaaa gagcccatct aagaaactcc tttgaataat taaatctaaa ttgggaatcg tcatcttcaa cataatggtc ttttaataat tggtaagtat cttcaaaatc ttgagattct atatcccata aattacaatc aaaccaagag aatccatctg gtagcttata tatttctttt ctaaaagaat ctggttcaat ttcaattggt ccaaatgaat attcagtgga agaatcatcg ttttgaacta ctggttgagt attccagaat ttgtgtggtt tgattgcgga tccaggaccc tgggtctgag cttccagggt acccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SAIKPHKFWN TQPVVQNDDS STEYSFGPIE IEPDSFRKEI YKLPDGFSWF DCNLWDIESQ DFEDTYQLLK DHYVEDDDSQ FRFNYSKEFL RWALCVPGQK KNWLVGVRVN ETKKMVGFIS AIPIKVRIHN CIMNTSVVNF LCVHKKLRSK RLAPVLIKEI TRRIRCEKIF QSIYTCGKNI TKPFTIGTYW HRIINVKKLL ETGFIGIPRN MTMSSLIKYH RIPADKRIEG FRPSVDSDAE QICKLFENYF MKYKDISNET MNNKINYDEI NHSKELGKQA YMKLDKIEDL QDKIIIHQCF NVEDVKHYFT NIDKVIVTYV RENKNKEITD LFSFFIIEST VINNERFPTI NIAYSYFNIA NTCSLKELFN EMIITAKNNN CDAFNTLDLM QNLQVIQDNK FIIGTGKLRY YVFNWKIPQI SPSNVGIILF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggatccgc aatcaaacca cacaaattct ggaatactca accagtagtt caaaacgatg attcttccac tgaatattca tttggaccaa ttgaaattga accagattct tttagaaaag aaatatataa gctaccagat ggattctctt ggtttgattg taatttatgg gatatagaat ctcaagattt tgaagatact taccaattat taaaagacca ttatgttgaa gatgacgatt cccaatttag atttaattat tcaaaggagt ttcttagatg ggctctttgt gtccctggtc aaaaaaaaaa ctggcttgtt ggagtcagag ttaatgaaac aaagaaaatg gttggattta tttcagcaat cccaatcaaa gttagaattc ataattgcat tatgaatact tctgtagtta attttctctg tgttcataag aagcttagat ctaaaagact tgcacctgta cttattaagg aaattacgag aagaattaga tgtgaaaaaa tctttcaatc aatttacact tgcgggaaga atattaccaa accttttact attggaactt attggcatcg tataattaat gtaaagaaat tattagaaac tggttttatt ggaatcccaa gaaatatgac catgagctct ttaatcaaat atcatcgtat tcctgctgat aaaaggattg aagggtttag accctccgta gattctgatg cagaacaaat atgtaaatta ttcgaaaatt actttatgaa atataaagat atcagcaacg aaaccatgaa taataaaata aactatgatg aaattaacca ctcaaaagag cttggaaaac aagcatatat gaagctggat aagattgaag atttgcaaga caaaatcata attcatcaat gctttaatgt tgaagatgtt aaacattatt tcacgaatat tgataaagtt attgtaactt atgttcgaga aaataaaaat aaggaaatta ctgacttgtt tagtttcttt atcatagaat ccactgtcat caataatgaa agatttccaa ctattaatat tgcttattca tactttaata ttgcaaatac ctgctcatta aaagaattgt tcaatgaaat gattattact gccaaaaata ataattgtga tgcttttaat acattggatc tgatgcaaaa tttacaagtt attcaagata ataaatttat tatcggtacc ggtaaattaa gatattatgt gtttaattgg aagatacctc aaatttcacc ttcaaatgtt ggaattatat tattttaact cgaggctccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for CrhoA.18219.a.B1.PW38435

PURIFICATION DATe:	2/21/2018
CONCENTRATION:	11.51mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	91

Protocol Notes

Combined pellets LSEID 43432 and 432433 Shipping all protein to CIDR

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTG GTGAAACCCC AGAAAAATCA GAATCTTCCA TTTCAAATAC CAAGAAGATC ACAAATCTTC TGAAAGAAAT GAGCTTAGGT AGCTTTATGA ACACTGCTGC AAATGCAATC AAACCACACA AATTCTGGAA TACTCAACCA GTAGTTCAAA ACGATGATTC TTCCACTGAA TATTCATTTG GACCAATTGA AATTGAACCA GATTCTTTTA GAAAAGAAAT ATATAAGCTA CCAGATGGAT TCTCTTGGTT TGATTGTAAT TTATGGGATA TAGAATCTCA AGATTTTGAA GATACTTACC AATTATTAAA AGACCATTAT GTTGAAGATG ACGATTCCCA ATTTAGATTT AATTATTCAA AGGAGTTTCT TAGATGGGCT CTTTGTGTCC CTGGTCAAAA AAAAAACTGG CTTGTTGGAG TCAGAGTTAA TGAAACAAAG AAAATGGTTG GATTTATTTC AGCAATCCCA ATCAAAGTTA GAATTCATAA TTGCATTATG AATACTTCTG TAGTTAATTT TCTCTGTGTT CATAAGAAGC TTAGATCTAA AAGACTTGCA CCTGTACTTA TTAAGGAAAT TACGAGAAGA ATTAGATGTG AAAAAATCTT TCAATCAATT TACACTTGCG GGAAGAATAT TACCAAACCT TTTACTATTG GAACTTATTG GCATCGTATA ATTAATGTAA AGAAATTATT AGAAACTGGT TTTATTGGAA TCCCAAGAAA TATGACCATG AGCTCTTTAA TCAAATATCA TCGTATTCCT GCTGATAAAA GGATTGAAGG GTTTAGACCC TCCGTAGATT CTGATGCAGA ACAAATATGT AAATTATTCG AAAATTACTT TATGAAATAT AAAGATATCA GCAACGAAAC CATGAATAAT AAAATAAACT ATGATGAAAT TAACCACTCA AAAGAGCTTG GAAAACAAGC ATATATGAAG CTGGATAAGA TTGAAGATTT GCAAGACAAA ATCATAATTC ATCAATGCTT TAATGTTGAA GATGTTAAAC ATTATTTCAC GAATATTGAT AAAGTTATTG TAACTTATGT TCGAGAAAAT AAAAATAAGG AAATTACTGA CTTGTTTAGT TTCTTTATCA TAGAATCCAC TGTCATCAAT AATGAAAGAT TTCCAACTAT TAATATTGCT TATTCATACT TTAATATTGC AAATACCTGC TCATTAAAAG AATTGTTCAA TGAAATGATT ATTACTGCCA AAAATAATAA TTGTGATGCT TTTAATACAT TGGATCTGAT GCAAAATTTA CAAGTTATTC AAGATAATAA ATTTATTATC GGTACCGGTA AATTAAGATA TTATGTGTTT AATTGGAAGA TACCTCAAAT TTCACCTTCA AATGTTGGAA TTATATTATT TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT

CENTER ID:	CrhoA.18219.a
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

CrhoA.18219.a

N-myristoyltransferase

Ordering Clones & Proteins

Clones*

Proteins

External Resources

Sequences

AA Sequence

NT Sequence

PURIFICATION DATe:	9/11/2012
CONCENTRATION:	29.3mg/ml
OBSERVED MW:	52kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	91