CrhoA.18217.a

topoisomerase I

CENTER ID: CrhoA.18217.a
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.18217.a.B1.GE35499 full length 1 641
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.70373
RefSeq: XP_668245.1
UniProt: Q5CN58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASATDSSLS NTSKRRKVMK KTEMDYEPLN KWWERPPKEE LGIQWEYLEH QGVMFAPPYK VHNVPLEYNG ERIQLPPEAE EIASYWSVMK DTEWAQKEKF SNNVRKAFMN SIPVGDKLHN NLEWEKCNFD IIKQYFEDEK EAKKNLSKEE KELQKKCRGD QESPFVYALV DWIKEKVGNF RIEPPGLFKG RGEHPKSGML KRRIFPEDVT LNIAEDAPVP KVPNSMKGHA WKDIFHDNTV TWLAYYRDSI NNQFKYIFLS ASSGFKGMSD YNKYEKARKL KDHIDAIRSD YRKKMTSGDI TQRQLGTATY LIDFLALRVG GEKDTDEEAD TVGCCSLRVE HITFNQQEKS ITLDFLGKDS IRYYNTVVID PSAFNNLTIF CRNKDKMENV FDQINMSSLN QYLKSIMPEL SAKVFRTFNA SITLERELSK ISAKSAVKSE KKTKMELESC LKDELNLSNF NNEVDITNVN DILRFYNDAN REVAILCNHQ RSVPKQHESS MEKMIKQQQE LEEDLKICEA FLKILKKNNK SVTMNDLPKL STEVSFNGKQ RKYPINLNSS LDVVQKKIDS IKSKISNLYL KIKIKDDNKT VALNTSKINY MDPRISVAFC KKYDLPIDKI FNRSLRTKFP WAMYTRSDFS F
NT Sequence
atggcatcgg ctacggattc atcgttgagc aatacgagca agcggaggaa agttatgaag aaaacagaga tggattatga gcctctcaat aagtggtggg aaagacctcc gaaggaagag cttggaattc aatgggaata tcttgaacac caaggcgtga tgtttgctcc accatataaa gttcataatg taccattaga atataacggt gaacggattc aacttcctcc agaagcggaa gaaatcgcga gttattggtc agtcatgaaa gatacggagt gggctcaaaa agagaaattc agtaataatg taaggaaggc atttatgaac tccataccgg ttggtgacaa actacataat aatctggaat gggaaaaatg taattttgat attataaaac aatattttga ggatgagaaa gaagctaaaa aaaatttatc aaaggaagag aaagaactcc agaaaaaatg caggggagac caggaatctc catttgtata tgctcttgtt gattggatta aagaaaaggt tggaaatttc aggattgaac cacctggttt gtttaaaggc agaggagaac atccaaagtc tggtatgttg aagaggcgga tatttcctga agatgtaact ttgaatattg cggaggatgc acctgttcca aaggtaccta atagtatgaa aggacatgct tggaaggata tattccatga taatacagtt acttggctcg cgtactatag agactctata aataatcaat ttaaatatat tttcctttct gcttcaagcg ggtttaaagg gatgagcgat tataataagt acgaaaaggc aagaaagtta aaagatcata ttgatgcaat acgcagtgac tataggaaaa aaatgacaag cggagatatc actcagaggc agcttggaac agcaacatat ttgatagatt ttttagcttt gagagtcgga ggagaaaaag atacagatga ggaagctgat actgttggct gctgctcatt aagagtagaa catattacat ttaatcaaca agaaaaatca attactttgg attttttggg caaagattca attcgatact ataatactgt cgttattgat ccctcagcat tcaataattt aacaattttc tgcagaaaca aggataagat ggaaaatgtc tttgatcaga ttaatatgag ctccctaaac caatatctaa aatctattat gcccgaactt tctgccaagg tatttagaac attcaatgca tcaattacat tagaacgtga gctttcaaaa atttcagcaa agtcagctgt gaaaagtgaa aaaaaaacca aaatggaact tgaatcatgc ttaaaagatg aacttaattt atctaatttt aataatgaag tggatattac caatgttaac gatattttaa gattttacaa tgatgctaat agagaagttg cgatattatg taaccatcag agatcagttc ctaaacaaca tgaatcttcc atggaaaaaa tgattaaaca acaacaggag ttagaagaag atttaaaaat ttgtgaagca ttcctcaaga ttttgaagaa aaataacaaa tctgttacaa tgaatgactt gccaaaacta tctactgagg tatctttcaa tggaaaacag agaaaatacc caataaactt gaactcaagt ctagatgtag tgcaaaaaaa aatagattct attaagagca aaatttcaaa tttgtactta aagatcaaaa taaaggatga taataagact gtagcgttaa atacatcgaa gattaactat atggatccca ggatttctgt tgcattttgt aagaaatacg atttaccaat cgataaaata tttaatagga gcttaagaac aaagttccct tgggcaatgt atacgagatc agatttttca ttttaa
Details for CrhoA.18217.a.B1.GE35499
HARVESTED ON: 6/26/2012
SEQUENCED ON: 7/3/2012
EXPECTED MW: 75kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 30
Validated AA Sequence
MXKTEMDYEP LNKWWERPPK EELGIXXEYL EHQGXMFAPP YKVHNVPLEY NGERIXLPPE AEEIXSYWSV MKDTXWAQKE KFSNNVXKAF MNSXPVGDKL HNNLEWEKCN FDIIKQYFED EKEAKKNLSK EEKELXKKCR GDQESPFVYA LVDWIKEKVG NFXIEPPGLF KGRGEHPKSG MLXXRIFPXX VTXNIA
Validated NT Sequence
gaaagttatg aanaaaacag agatggatta tgagcctctc aataagtggt gggaaagacc tccgaaggaa gagcttggaa ttcnnnggga atatcttgaa caccaaggnn ngatgtttgc tccaccatat aaagttcata atgtaccatt agaatataac ggtgaacgga ttcnacttcc tccagaagcg gaagaaatcn cgagttattg gtcagtcatg aaagatacgg antgggctca aaaagagaaa ttcagtaata atgtanggaa ggcatttatg aactccntac cggttggtga caaactacat aataatctgg aatgggaaaa atgtaatttt gatattataa aacaatattt tgaggatgag aaagaagcta aaaaaaattt atcaaaggaa gagaaagaac tccanaaaaa atgcagggga gaccaggaat ctccatttgt atatgctctt gttgattgga ttaaagaaaa ggttggaaat ttcnngattg aaccacctgg nttgtttaaa ggcagaggag aacatccaaa gtctggtatg ttgaanangc ggatatttcc tgannatgta actttnaata ttgcnga
Expected Protein Sequence
MAHHHHHHMA SATDSSLSNT SKRRKVMKKT EMDYEPLNKW WERPPKEELG IQWEYLEHQG VMFAPPYKVH NVPLEYNGER IQLPPEAEEI ASYWSVMKDT EWAQKEKFSN NVRKAFMNSI PVGDKLHNNL EWEKCNFDII KQYFEDEKEA KKNLSKEEKE LQKKCRGDQE SPFVYALVDW IKEKVGNFRI EPPGLFKGRG EHPKSGMLKR RIFPEDVTLN IAEDAPVPKV PNSMKGHAWK DIFHDNTVTW LAYYRDSINN QFKYIFLSAS SGFKGMSDYN KYEKARKLKD HIDAIRSDYR KKMTSGDITQ RQLGTATYLI DFLALRVGGE KDTDEEADTV GCCSLRVEHI TFNQQEKSIT LDFLGKDSIR YYNTVVIDPS AFNNLTIFCR NKDKMENVFD QINMSSLNQY LKSIMPELSA KVFRTFNASI TLERELSKIS AKSAVKSEKK TKMELESCLK DELNLSNFNN EVDITNVNDI LRFYNDANRE VAILCNHQRS VPKQHESSME KMIKQQQELE EDLKICEAFL KILKKNNKSV TMNDLPKLST EVSFNGKQRK YPINLNSSLD VVQKKIDSIK SKISNLYLKI KIKDDNKTVA LNTSKINYMD PRISVAFCKK YDLPIDKIFN RSLRTKFPWA MYTRSDFSF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat cggctacgga ttcatcgttg agcaatacga gcaagcggag gaaagttatg aagaaaacag agatggatta tgagcctctc aataagtggt gggaaagacc tccgaaggaa gagcttggaa ttcaatggga atatcttgaa caccaaggcg tgatgtttgc tccaccatat aaagttcata atgtaccatt agaatataac ggtgaacgga ttcaacttcc tccagaagcg gaagaaatcg cgagttattg gtcagtcatg aaagatacgg agtgggctca aaaagagaaa ttcagtaata atgtaaggaa ggcatttatg aactccatac cggttggtga caaactacat aataatctgg aatgggaaaa atgtaatttt gatattataa aacaatattt tgaggatgag aaagaagcta aaaaaaattt atcaaaggaa gagaaagaac tccagaaaaa atgcagggga gaccaggaat ctccatttgt atatgctctt gttgattgga ttaaagaaaa ggttggaaat ttcaggattg aaccacctgg tttgtttaaa ggcagaggag aacatccaaa gtctggtatg ttgaagaggc ggatatttcc tgaagatgta actttgaata ttgcggagga tgcacctgtt ccaaaggtac ctaatagtat gaaaggacat gcttggaagg atatattcca tgataataca gttacttggc tcgcgtacta tagagactct ataaataatc aatttaaata tattttcctt tctgcttcaa gcgggtttaa agggatgagc gattataata agtacgaaaa ggcaagaaag ttaaaagatc atattgatgc aatacgcagt gactatagga aaaaaatgac aagcggagat atcactcaga ggcagcttgg aacagcaaca tatttgatag attttttagc tttgagagtc ggaggagaaa aagatacaga tgaggaagct gatactgttg gctgctgctc attaagagta gaacatatta catttaatca acaagaaaaa tcaattactt tggatttttt gggcaaagat tcaattcgat actataatac tgtcgttatt gatccctcag cattcaataa tttaacaatt ttctgcagaa acaaggataa gatggaaaat gtctttgatc agattaatat gagctcccta aaccaatatc taaaatctat tatgcccgaa ctttctgcca aggtatttag aacattcaat gcatcaatta cattagaacg tgagctttca aaaatttcag caaagtcagc tgtgaaaagt gaaaaaaaaa ccaaaatgga acttgaatca tgcttaaaag atgaacttaa tttatctaat tttaataatg aagtggatat taccaatgtt aacgatattt taagatttta caatgatgct aatagagaag ttgcgatatt atgtaaccat cagagatcag ttcctaaaca acatgaatct tccatggaaa aaatgattaa acaacaacag gagttagaag aagatttaaa aatttgtgaa gcattcctca agattttgaa gaaaaataac aaatctgtta caatgaatga cttgccaaaa ctatctactg aggtatcttt caatggaaaa cagagaaaat acccaataaa cttgaactca agtctagatg tagtgcaaaa aaaaatagat tctattaaga gcaaaatttc aaatttgtac ttaaagatca aaataaagga tgataataag actgtagcgt taaatacatc gaagattaac tatatggatc ccaggatttc tgttgcattt tgtaagaaat acgatttacc aatcgataaa atatttaata ggagcttaag aacaaagttc ccttgggcaa tgtatacgag atcagatttt tcattttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t