CrhoA.18216.a

ruvB-like 2 protein, putative

CENTER ID: CrhoA.18216.a
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.18216.a.B1.GE35498 full length 1 479
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.50324
RefSeq: XP_667311.1
UniProt: Q5CKF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIEPKIAEIK EFQRLERIGA HSHIRGLGLN DALDARYSSD GMVGQKLARR AAGIIVRMVK QGKIAGRAIL LSGQPGTGKT AIAMAIAKAI GSDTPFTHIS ASEVFSLEMN KTEALRQALR RSIGVRIKEE IDVIEGEVAE LEIDRSNTTG VKVGRMALRS TDMETVYDIG SKMIESLQAE NIVAGDVISI NKSSGKITKL GRSFTRSKDY DAVGYQTRFI ACPEGELQKK REVVHNVTLH DIDVINSRTQ GFLALFAGDT GEIKPEVRAQ IDEKVAEWKE ESRAEIVHGV LFIDEVHMLD VECFSFLNKA LEEETSPILI MASNRGITKI RGTDYKSPHG IPIDLLDRCL IIPTVPYSEE DVKKIIQERA SEEDLKLTDS AYQILTRIAM DTSLRYSLHL LTVSQVLANR KKKEEIDLDE IKKAYSLFID VKRSTQYLID YQQEYLFSEI EDSNNDVKDK DNSNDTMEDT AVQEKMTLM
NT Sequence
atgattgaac caaaaatagc agaaattaaa gagtttcaaa gactggaaag aattggggca cattcccata ttagaggcct aggtttaaat gatgcattgg atgcaaggta ttcatcagat ggaatggtgg gtcaaaagtt ggccagaaga gctgcaggaa taattgtaag aatggtcaaa cagggcaaga ttgcaggaag agcaatttta ttatcaggtc agccaggaac tggaaaaaca gcaattgcta tggcaattgc aaaagcaata ggaagcgata cgccatttac acatatttca gcttctgaag tgttctcact agaaatgaat aagactgaag ctctgagaca agctttgaga agatccatag gtgtaagaat taaagaagaa atagacgtta ttgagggaga agtagcagaa ttagagattg atcgttcgaa tacaacagga gttaaagttg gcagaatggc tttaagaagc actgatatgg agacagttta tgatattggt agtaaaatga ttgagtcact tcaagctgaa aatattgttg ctggagatgt aatttcaatt aataagtcaa gtgggaaaat tactaaatta ggacgttctt ttacaaggtc taaagattac gatgctgttg gatatcaaac tagatttatt gcatgcccag aaggtgaact tcagaaaaaa agagaagtag tacataacgt cactttacac gatattgatg taattaatag ccgaactcaa ggatttttag cactgtttgc tggtgacacc ggcgaaatta agccagaagt tagagctcaa atagatgaaa aggttgctga atggaaagag gaaagcagag cagaaattgt tcatggagtt ttgtttatag atgaggttca tatgttagat gttgaatgct ttagctttct taacaaggcg ctagaggaag aaacaagtcc aatcttgatt atggcatcaa atagaggtat tacaaagatt agaggaactg actataagtc gccacatggt atacctattg atcttttaga tagatgttta attattccaa cagtacccta tagtgaagaa gatgttaaga aaattattca ggaaagagca agtgaggagg atcttaaatt aactgatagt gcttatcaaa tcctcactag aatagcgatg gacacgagct tgcgatactc actacactta ttaacagttt ctcaagttct tgcaaataga aaaaaaaaag aagagattga tttagacgag attaaaaaag cctattcact ttttattgat gttaagagaa gtactcaata tcttattgat taccaacaag agtatctatt ttctgagatt gaagattcaa ataatgacgt gaaagataaa gataatagca atgatacaat ggaagacact gcagttcagg agaaaatgac attaatgtag
Details for CrhoA.18216.a.B1.GE35498
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 54kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 61
Validated AA Sequence
MAHXHHHHMI EPKIAEIKEF QRLERIGAHS HIRGLGLNDA LDARYSSDGM VGQKLARRAA GIIVRMVKQG KIAGRAILLS GQPGTGKTAI AMAIAKAIGS DTPFTHISAS EVFSLETNKT EALRQALRRS IGVRIKEEID VIEGEVAELE IDRSNTTGVK VGRMALRSTD METVYDIGSK MIESLQAENI VAGDVISINK SSGKITKLGR SFTRSKDYDA VGYQTRFIAC PEGELQKKRE VVHNVTLHDI DVINSRTQGF LALFAGDTGE IKPEVRAQID EKVLNGKREA EQKLFMEFCL
Validated NT Sequence
attnnnnnnn nnnnnngaag nnnnnnnacc atggctcacc nccaccacca ccatatgatt gaaccaaaaa tagcagaaat taaagagttt caaagactgg aaagaattgg ggcacattcc catattagag gcctaggttt aaatgatgca ttggatgcaa ggtattcatc agatggaatg gtgggtcaaa agttggccag aagagctgca ggaataattg taagaatggt caaacagggc aagattgcag gaagagcaat tttattatca ggtcagccag gaactggaaa aacagcaatt gctatggcaa ttgcaaaagc aataggaagc gatacgccat ttacacatat ttcagcttct gaagtgttct cactagaaac gaataagact gaagctctga gacaagcttt gagaagatcc ataggtgtaa gaattaaaga agaaatagac gttattgagg gagaagtagc agaattagag attgatcgtt cgaatacaac aggagttaaa gttggcagaa tggctttaag aagcactgat atggagacag tttatgatat tggtagtaaa atgattgagt cacttcaagc tgaaaatatt gttgctggag atgtaatttc aattaataag tcaagtggga aaattactaa attaggacgt tcttttacaa ggtctaaaga ttacgatgct gttggatatc aaactagatt tattgcatgc ccagaaggtg aacttcagaa aaaaagagaa gtagtacata acgtcacttt acacgatatt gatgtaatta atagccgaac tcaaggattt ttagcactgt ttgctggtga caccggcgaa attaagccag aagttagagc tcaaatagat gaaaaggtgc tgaatggaaa gagggaagca gagcagaaat tgttcatgga gttttgttta tagatgaggn tcatatgtta gatgttgaat gctttagctt tcttaacnng gngctagagg aagaaanant ccnatcttga ttatggcatc aantagaggt antacaanat tannggantg actnnnagtc gccnnatggn nnacctantg atcntttnna nnnatgntnn ntnnntnnnn ngtaccctnt aannnnnnnn aaannnntnn nnannnnnca nnnnnngnnn nannntnann nnnnnnngnt nnnnnnnnnc nnnnnnnnnn ncnnnnnnnn nnnnannnnn nnnnnnnna
Expected Protein Sequence
MAHHHHHHMI EPKIAEIKEF QRLERIGAHS HIRGLGLNDA LDARYSSDGM VGQKLARRAA GIIVRMVKQG KIAGRAILLS GQPGTGKTAI AMAIAKAIGS DTPFTHISAS EVFSLEMNKT EALRQALRRS IGVRIKEEID VIEGEVAELE IDRSNTTGVK VGRMALRSTD METVYDIGSK MIESLQAENI VAGDVISINK SSGKITKLGR SFTRSKDYDA VGYQTRFIAC PEGELQKKRE VVHNVTLHDI DVINSRTQGF LALFAGDTGE IKPEVRAQID EKVAEWKEES RAEIVHGVLF IDEVHMLDVE CFSFLNKALE EETSPILIMA SNRGITKIRG TDYKSPHGIP IDLLDRCLII PTVPYSEEDV KKIIQERASE EDLKLTDSAY QILTRIAMDT SLRYSLHLLT VSQVLANRKK KEEIDLDEIK KAYSLFIDVK RSTQYLIDYQ QEYLFSEIED SNNDVKDKDN SNDTMEDTAV QEKMTLM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg aaccaaaaat agcagaaatt aaagagtttc aaagactgga aagaattggg gcacattccc atattagagg cctaggttta aatgatgcat tggatgcaag gtattcatca gatggaatgg tgggtcaaaa gttggccaga agagctgcag gaataattgt aagaatggtc aaacagggca agattgcagg aagagcaatt ttattatcag gtcagccagg aactggaaaa acagcaattg ctatggcaat tgcaaaagca ataggaagcg atacgccatt tacacatatt tcagcttctg aagtgttctc actagaaatg aataagactg aagctctgag acaagctttg agaagatcca taggtgtaag aattaaagaa gaaatagacg ttattgaggg agaagtagca gaattagaga ttgatcgttc gaatacaaca ggagttaaag ttggcagaat ggctttaaga agcactgata tggagacagt ttatgatatt ggtagtaaaa tgattgagtc acttcaagct gaaaatattg ttgctggaga tgtaatttca attaataagt caagtgggaa aattactaaa ttaggacgtt cttttacaag gtctaaagat tacgatgctg ttggatatca aactagattt attgcatgcc cagaaggtga acttcagaaa aaaagagaag tagtacataa cgtcacttta cacgatattg atgtaattaa tagccgaact caaggatttt tagcactgtt tgctggtgac accggcgaaa ttaagccaga agttagagct caaatagatg aaaaggttgc tgaatggaaa gaggaaagca gagcagaaat tgttcatgga gttttgttta tagatgaggt tcatatgtta gatgttgaat gctttagctt tcttaacaag gcgctagagg aagaaacaag tccaatcttg attatggcat caaatagagg tattacaaag attagaggaa ctgactataa gtcgccacat ggtataccta ttgatctttt agatagatgt ttaattattc caacagtacc ctatagtgaa gaagatgtta agaaaattat tcaggaaaga gcaagtgagg aggatcttaa attaactgat agtgcttatc aaatcctcac tagaatagcg atggacacga gcttgcgata ctcactacac ttattaacag tttctcaagt tcttgcaaat agaaaaaaaa aagaagagat tgatttagac gagattaaaa aagcctattc actttttatt gatgttaaga gaagtactca atatcttatt gattaccaac aagagtatct attttctgag attgaagatt caaataatga cgtgaaagat aaagataata gcaatgatac aatggaagac actgcagttc aggagaaaat gacattaatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat