CrhoA.17908.a

ubiquinone/menaquinone biosynthesis methyltransferae, putative

CENTER ID:	CrhoA.17908.a
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.17908.a.B1.GE35511	full length		1	221

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:Chro.10320
OrthoMCL:	OG5_127211
RefSeq:	XP_668372.1
UniProt:	Q5CNI5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNLRNISKS GIGYSFCRLL VRKTHNLFGF KFLNIGINQI NLARAARFNS TIKDSNTGEN NKYVYFGDRI VDKDTKRKLI EQVFSSVSNK YDLMNDLMSL GLHRCWKDIF VENIISPESF LRIKREKDED FSNVTILDVA GGTGDISFRI IDKFRKDNIE VLPNIIVVDS NNEMIEQGQL KSIEKGDCSI IWIKDDGEQL SQIPSNSVDV VAISFGIRNF T

NT Sequence

atgaacaatt taagaaatat atcaaaaagt ggtattgggt atagtttttg tcgcttgtta gtcaggaaaa cacataattt gtttggattc aaatttttga atattggcat taatcaaatt aatcttgcaa gagcagctag gtttaattca accattaaag actcgaacac tggtgaaaat aacaaatacg tttactttgg ggatcgtatt gtggacaaag atactaaaag gaagctaatt gaacaggttt ttagctcagt ttcgaataaa tatgatttaa tgaatgattt gatgagtctt ggattacata ggtgttggaa ggacatattt gtagagaata tcatttcacc agaatcattt ttgagaataa aaagagagaa ggacgaagat ttctcaaatg taactatttt ggatgttgct ggaggaacag gagatatttc ttttagaatt attgataagt tcagaaaaga taatattgag gtcttaccaa atattatagt tgtcgactct aataatgaaa tgattgagca aggacagcta aaatcaattg aaaaaggaga ctgcagcatt atttggatta aagatgatgg agaacaactt agtcaaattc ctagtaacag tgtagatgtt gttgcgatat cgtttggaat tcgcaacttt aca

Details for CrhoA.17908.a.B1.GE35511

HARVESTED ON:	data unavailable
SEQUENCED ON:	7/3/2012
EXPECTED MW:	26kDa
OBSERVED MW:	21kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	88

Validated AA Sequence

MAHHHHHHMN NLRNISKSGI GYSFCRLLVR KTHNLFGFKF LNIGINQINL ARAARFNSTI KDSNTGENNK YVYFGDRIVD KDTKRKLIEQ VFSSVSNKYD LMNDLMSLGL HRCWKDIFVE NIISPESFLR IKREKDEDFS NVTILDVAGG TGDISFRIID KFRKDNIEVL PNIIVVDSNN EMIEQGQLKS IEKGDCSIIW KR

Validated NT Sequence

ttaagaagga ganataccat ggctcaccac caccaccacc atatgaacaa tttaagaaat atatcaaaaa gtggtattgg gtatagtttt tgtcgcttgt tagtcaggaa aacacataat ttgtttggat tcaaattttt gaatattggc attaatcaaa ttaatcttgc aagagcagct aggtttaatt caaccattaa agactcgaac actggtgaaa ataacaaata cgtttacttt ggggatcgta ttgtggacaa agatactaaa aggaagctaa ttgaacaggt ttttagctca gtttcgaata aatatgattt aatgaatgat ttgatgagtc ttggattaca taggtgttgg aaggacatat ttgtagagaa tatcatttca ccagaatcat ttttgagaat aaaaagagag aaggacgaag atttctcaaa tgtaactatt ttggatgttg ctggaggaac aggagatatt tcttttagaa ttattgataa gttcagaaaa gataatattg aggtcttacc aaatattata gttgtcgact ctaataatga aatgattgag caaggacagc taaaatcaat tgaaaaagga gactgcagca ttatttggaa aagatgatgg agaacaactt agtcaaattc ctagtaacag tgtagatgtt gttgcgatat cgtttggaat tcgcaacttt acataagtga gtaagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca atttaagaaa tatatcaaaa agtggtattg ggtatagttt ttgtcgcttg ttagtcagga aaacacataa tttgtttgga ttcaaatttt tgaatattgg cattaatcaa attaatcttg caagagcagc taggtttaat tcaaccatta aagactcgaa cactggtgaa aataacaaat acgtttactt tggggatcgt attgtggaca aagatactaa aaggaagcta attgaacagg tttttagctc agtttcgaat aaatatgatt taatgaatga tttgatgagt cttggattac ataggtgttg gaaggacata tttgtagaga atatcatttc accagaatca tttttgagaa taaaaagaga gaaggacgaa gatttctcaa atgtaactat tttggatgtt gctggaggaa caggagatat ttcttttaga attattgata agttcagaaa agataatatt gaggtcttac caaatattat agttgtcgac tctaataatg aaatgattga gcaaggacag ctaaaatcaa ttgaaaaagg agactgcagc attatttgga ttaaagatga tggagaacaa cttagtcaaa ttcctagtaa cagtgtagat gttgttgcga tatcgtttgg aattcgcaac tttacatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t