CrhoA.17369.c

ATP synthase beta chain, putative

CENTER ID: CrhoA.17369.c
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.17369.c.B2.GE35449 mature protein 21 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.20148
RefSeq: XP_668104.1
UniProt: Q5CMN8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFMIRGKLLS QSLPCFKFTR REFLPLFSVF FQKKAIRNLE YYNLNKRWKS YSKTSKNLSE GYISQIMGSV VDVKFEGKLP ELLNALEVKG HQNKLVLEVA QHLSDNSVRA IAMDVTEGLS RGEKVIDTGS PICVPVGKAT LGRMVNVMGN AIDGCGEIDA KVKKPIHRTA PEYMEQVMEP SLIVTGIKAI DLLTPFIKGG KIGLFGGAGV GKTVLIMELI NNIAKKYGGY SVYTGVGERI REGYDLYNEM LANGVNKKSI VGSRVDSKHK RQPIYDFLGS KTALVYGQMN ETPGARARVA LTGLTMAEYF RDSMMQDVLF FVDNIYRFTQ AGSEVSALLG K
NT Sequence
atgtttatga ttcgaggaaa attgttaagt caatctcttc catgcttcaa attcacacga agagaatttt taccactatt ttcggtattt ttccagaaaa aagcgatccg caacctagaa tattataact taaacaaaag atggaagtct tatagtaaaa ctagcaaaaa tctctcagaa ggctatatat cacaaatcat gggttcagtt gtagatgtaa aatttgaggg taaactacca gaattgttaa acgctttgga agttaaaggg catcaaaaca aactagtact tgaagtggcg caacacttaa gtgataattc agttagggca attgcaatgg atgttacaga aggactctct agaggagaga aagttataga cactggaagc ccaatatgcg ttccagtagg caaggccaca cttggtagaa tggtgaatgt tatggggaat gcaattgacg gatgtggaga gatcgacgct aaagttaaaa aaccgattca tagaacggcc cctgaataca tggaacaagt aatggagcct tcattaattg ttactgggat taaggcaata gatctactta caccattcat aaaaggtggg aaaataggac tatttggtgg agcgggggtt ggcaagactg ttcttatcat ggaactaata aacaatattg caaaaaagta tggtggttac tctgtctata caggggttgg agagcgcatc agggaagggt acgatttata taatgaaatg ctagcaaatg gcgtcaataa aaagtcgatt gttggatcac gagttgacag caagcataag agacaaccca tatacgattt tttaggttct aagacagcgc tggtatatgg tcaaatgaat gaaactccag gagcgcgagc gcgagttgca cttacaggtc taacaatggc agaatatttt agagattcaa tgatgcaaga cgtattattt ttcgtagata atatttaccg atttactcag gcagggagtg aagtttctgc acttttaggt aagtaa
Details for CrhoA.17369.c.B2.GE35449
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMR EFLPLFSVFF QKKAIRNLEY YNLNKRWKSY SKTSKNLSEG YISQIMGSVV DVKFEGKLPE LLNALEVKGH QNKLVLEVAQ HLSDNSVRAI AMDVTEGLSR GEKVIDTGSP ICVPVGKATL GRMVNVMGNA IDGCGEIDAK VKKPIHRTAP EYMEQVMEPS LIVTGIKAID LLTPFIKGGK IGLFGGAGVG KTVLIMELIN NIAKKYGGYS VYTGVGERIX EGYDLYNEML ANGVXKKSIV GSRVDSKHKR QPIYDFLGSK TALVYGQMNE TPGARARVAL TGLTMAXILE XQ
Validated NT Sequence
ttttgtttan ctttaagaag gaganatacc atggctcacc accaccacca ccatatgaga gaatttttac cactattttc ggtatttttc cagaaaaaag cgatccgcaa cctagaatat tataacttaa acaaaagatg gaagtcttat agtaaaacta gcaaaaatct ctcagaaggc tatatatcac aaatcatggg ttcagttgta gatgtaaaat ttgagggtaa actaccagaa ttgttaaacg ctttggaagt taaagggcat caaaacaaac tagtacttga agtggcgcaa cacttaagtg ataattcagt tagggcaatt gcaatggatg ttacagaagg actctctaga ggagagaaag ttatagacac tggaagccca atatgcgttc cagtaggcaa ggccacactt ggtagaatgg tgaatgttat ggggaatgca attgacggat gtggagagat cgacgctaaa gttaaaaaac cgattcatag aacggcccct gaatacatgg aacaagtaat ggagccttca ttaattgtta ctgggattaa ggcaatagat ctacttacac cattcataaa aggtgggaaa ataggactat ttggtggagc gggggttggc aagactgttc ttatcatgga actaataaac aatattgcaa aaaagtatgg tggttactct gtctatacag gggttggaga gcgcatcang gaagggtacg atttatataa tgaaatgcta gcaaatggcg tcnataaaaa gtcgattgtt ggatcacgag ttgacagcaa gcataagaga caacccatat acgatttttt aggttctaag acagcgctgg tatatggtca aatgaatgaa actccnggag cgcgagcgcg agttgcactt acaggtctaa caatggcaga nattttagag antcaatgat gcaagacgta ttatttttcg tanannnatt taccgantta ctcnnnnngn ntgaagttnc tgcnctttag gtaagtaagt gagtannang anccnnnnnn nnnnancccc
Expected Protein Sequence
MAHHHHHHMR EFLPLFSVFF QKKAIRNLEY YNLNKRWKSY SKTSKNLSEG YISQIMGSVV DVKFEGKLPE LLNALEVKGH QNKLVLEVAQ HLSDNSVRAI AMDVTEGLSR GEKVIDTGSP ICVPVGKATL GRMVNVMGNA IDGCGEIDAK VKKPIHRTAP EYMEQVMEPS LIVTGIKAID LLTPFIKGGK IGLFGGAGVG KTVLIMELIN NIAKKYGGYS VYTGVGERIR EGYDLYNEML ANGVNKKSIV GSRVDSKHKR QPIYDFLGSK TALVYGQMNE TPGARARVAL TGLTMAEYFR DSMMQDVLFF VDNIYRFTQA GSEVSALLGK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagag aatttttacc actattttcg gtatttttcc agaaaaaagc gatccgcaac ctagaatatt ataacttaaa caaaagatgg aagtcttata gtaaaactag caaaaatctc tcagaaggct atatatcaca aatcatgggt tcagttgtag atgtaaaatt tgagggtaaa ctaccagaat tgttaaacgc tttggaagtt aaagggcatc aaaacaaact agtacttgaa gtggcgcaac acttaagtga taattcagtt agggcaattg caatggatgt tacagaagga ctctctagag gagagaaagt tatagacact ggaagcccaa tatgcgttcc agtaggcaag gccacacttg gtagaatggt gaatgttatg gggaatgcaa ttgacggatg tggagagatc gacgctaaag ttaaaaaacc gattcataga acggcccctg aatacatgga acaagtaatg gagccttcat taattgttac tgggattaag gcaatagatc tacttacacc attcataaaa ggtgggaaaa taggactatt tggtggagcg ggggttggca agactgttct tatcatggaa ctaataaaca atattgcaaa aaagtatggt ggttactctg tctatacagg ggttggagag cgcatcaggg aagggtacga tttatataat gaaatgctag caaatggcgt caataaaaag tcgattgttg gatcacgagt tgacagcaag cataagagac aacccatata cgatttttta ggttctaaga cagcgctggt atatggtcaa atgaatgaaa ctccaggagc gcgagcgcga gttgcactta caggtctaac aatggcagaa tattttagag attcaatgat gcaagacgta ttatttttcg tagataatat ttaccgattt actcaggcag ggagtgaagt ttctgcactt ttaggtaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat