SSGCID: Target Page

CrhoA.17369.b

vacuolar ATP synthase subunit b

CENTER ID:	CrhoA.17369.b
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.17369.b.B1.GE35448	full length		1	493

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:Chro.80195
RefSeq:	XP_666885.1
UniProt:	Q5CJ77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVTSGQAYEA NVAAVTRNYN IAPRMEYRTV SGVQGPLVIL DNVKFPKYNE IVRITLGDGS VREGQVLEVK GSRAVVQVFE GTTGIDNQSC RVEFTGDVLC MPITEDMLGR TFNGSGKPID NGPPIFAEDY IDINGSPINP YCRVHPREMI QTGISAIDVM NSVVRGQKIP LFSAAGLPHN DIGAQICRQA SLVSGKDVMD HSDDNFAVVF AAMGVNMETA RFFRRDFEEN GSMERVCLFL NLANDPTIER ILTPKLALTT AEYLAYEKDL HVFVVMTDMS SYADALREVS AAREEVPGRR GYPGYMYTDL STIYERAGRI EGRNGSITQF PILTMPNDDI THPIPDLTGY ITEGQIFVDR ALYNRQIYPP INVLPSLSRL MKSGIGTGLT REDHPSVSDQ LYANYAIGQD TRAMVAVVGE EALSADDLLY LEFTSKFESK FLSQGPYEKR DIFESLDLAW ELLRTFPEDM LKKIKHDLLQ KYYPRDNYIQ HSK

NT Sequence

atggtaactt caggccaggc ttatgaggca aatgtagcag cagttacacg aaactataat atcgctccta gaatggagta tagaacagtt tcaggagttc aaggtccatt agtaatttta gataatgtaa agtttccaaa atacaatgag attgtaagaa ttactttggg agatggttct gttagagaag gacaagtttt ggaggtcaaa ggaagcagag ctgttgtgca agtatttgag ggtactacag gcattgacaa tcaatcatgt agagttgaat ttactgggga tgtattgtgt atgccaataa cagaagatat gttgggtaga acatttaatg gaagcggaaa accaattgat aatggccctc caatttttgc tgaagattat attgatatta atggtagtcc aattaatcca tactgtagag ttcatccaag agaaatgatt cagacaggaa tttcagcaat tgatgttatg aactcagttg ttagaggtca gaagattcca ttattttctg ctgcaggact tccacataat gatattggtg cacaaatttg tcgtcaagct tctctagtat ccggaaaaga tgttatggat cattctgatg ataattttgc tgtagtattt gctgctatgg gagttaatat ggagactgca agattcttta gacgtgattt tgaggaaaat ggttctatgg aaagagtatg cctctttttg aatttagcta atgatccaac tattgaaaga attttgactc caaaattggc attaacaaca gcagagtact tggcttatga gaaggattta catgtatttg ttgttatgac tgatatgagt tcttatgcag atgctcttag agaggtttct gctgcgagag aggaagttcc tggtagaaga ggttatcctg gttatatgta tacagatctt tcaactattt atgagagagc tggaagaatt gagggtagaa atggttcaat tactcaattc ccaattttaa caatgccaaa tgatgatatt acccatccaa ttccagatct tactggttac attacagaag gacagatctt tgttgataga gctctctata acagacagat ttatccacca attaatgttt taccatcatt atctcgtctt atgaagtctg gtattggtac tggtttaact agagaagatc atccatcagt ttctgatcaa ctttatgcta attatgctat tggccaggat actagagcta tggtagcagt tgtaggagag gaagctcttt cagctgatga tttattatat cttgagttca ctagtaaatt cgagtccaag ttcctttctc aaggtccata tgagaagaga gatatctttg agagtttgga tcttgcttgg gaacttctta gaacattccc tgaggatatg cttaagaaga ttaagcatga tttattacaa aagtattatc caagagacaa ttatattcaa cattctaagt aa

Details for CrhoA.17369.b.B1.GE35448

HARVESTED ON:	6/26/2012
SEQUENCED ON:	6/29/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	68

Validated AA Sequence

Validated NT Sequence

attttgtnnn nnttaagaag gaganatacc atggctcacc accaccacca ccatatggta acttcaggcc aggcttatga ggcaaatgta gcagcagtta cacgaaacta taatatcgct cctagaatgg agtatagaac agtttcagga gttcaaggtc cattagtaat tttagataat gtaaagtttc caaaatacaa tgagattgta agaattactt tgggagatgg ttctgttaga gaaggacaag ttttggaggt caaaggaagc agagctgttg tgcaagtatt tgagggtact acaggcattg acaatcaatc atgtagagtt gaatttactg gggatgtatt gtgtatgcca ataacagaag atatgttggg tagaacattt aatggaagcg gaaaaccaat tgataatggc cctccaattt ttgctgaaga ttatattgat attaatggta gtccaattaa tccatactgt agagttcatc caagagaaat gattcagaca ggaatttcag caattgatgt tatgaactca gttgttagag gtcagaagat tccattattt tctgctgcag gacttccaca taatgatatt ggtgcacaaa tttgtcgtca agcttctcta gtatccggaa aagatgttat ggatcattct gatgataatt ttgctgtagt atttgctgct atgggagtta atatggagac tgcaagattc tttagacgtg attttgagga aaatggttct atggaaagag tatgcctctt tttgaattta gctaatgatc caactattga aagaattttg actccaaaat tggcattaac aacagcagan tacttggctt atgagaagga tttacatgta tttgttgtta tgactgatat gagttcttat gcagatgctc ttagagaggt ttctgctgcg agagaggaag ttcctggtag aanagnntnt cctggttata tgtatacnga tctttcanct atttatgaga ganctggnan aantgnnggt agaaanggnt tcnnactcan ttnccnnttt tnncnntgnn antgatgann ntaccnncnc nnannntnct gnnnnnnnnn nnnnnnnnna nnnttnntga nnnanntnnn nnnnnncnna nnnnnntnnn nnnnnnnnnn nngntnnnnn ncnnnannnc nnnnnnnnnn nnnnnnnnnn nnnnngnnnt nnnnnnnnnn annnannnnn nnnnnnnnt

Expected Protein Sequence

MAHHHHHHMV TSGQAYEANV AAVTRNYNIA PRMEYRTVSG VQGPLVILDN VKFPKYNEIV RITLGDGSVR EGQVLEVKGS RAVVQVFEGT TGIDNQSCRV EFTGDVLCMP ITEDMLGRTF NGSGKPIDNG PPIFAEDYID INGSPINPYC RVHPREMIQT GISAIDVMNS VVRGQKIPLF SAAGLPHNDI GAQICRQASL VSGKDVMDHS DDNFAVVFAA MGVNMETARF FRRDFEENGS MERVCLFLNL ANDPTIERIL TPKLALTTAE YLAYEKDLHV FVVMTDMSSY ADALREVSAA REEVPGRRGY PGYMYTDLST IYERAGRIEG RNGSITQFPI LTMPNDDITH PIPDLTGYIT EGQIFVDRAL YNRQIYPPIN VLPSLSRLMK SGIGTGLTRE DHPSVSDQLY ANYAIGQDTR AMVAVVGEEA LSADDLLYLE FTSKFESKFL SQGPYEKRDI FESLDLAWEL LRTFPEDMLK KIKHDLLQKY YPRDNYIQHS K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtaa cttcaggcca ggcttatgag gcaaatgtag cagcagttac acgaaactat aatatcgctc ctagaatgga gtatagaaca gtttcaggag ttcaaggtcc attagtaatt ttagataatg taaagtttcc aaaatacaat gagattgtaa gaattacttt gggagatggt tctgttagag aaggacaagt tttggaggtc aaaggaagca gagctgttgt gcaagtattt gagggtacta caggcattga caatcaatca tgtagagttg aatttactgg ggatgtattg tgtatgccaa taacagaaga tatgttgggt agaacattta atggaagcgg aaaaccaatt gataatggcc ctccaatttt tgctgaagat tatattgata ttaatggtag tccaattaat ccatactgta gagttcatcc aagagaaatg attcagacag gaatttcagc aattgatgtt atgaactcag ttgttagagg tcagaagatt ccattatttt ctgctgcagg acttccacat aatgatattg gtgcacaaat ttgtcgtcaa gcttctctag tatccggaaa agatgttatg gatcattctg atgataattt tgctgtagta tttgctgcta tgggagttaa tatggagact gcaagattct ttagacgtga ttttgaggaa aatggttcta tggaaagagt atgcctcttt ttgaatttag ctaatgatcc aactattgaa agaattttga ctccaaaatt ggcattaaca acagcagagt acttggctta tgagaaggat ttacatgtat ttgttgttat gactgatatg agttcttatg cagatgctct tagagaggtt tctgctgcga gagaggaagt tcctggtaga agaggttatc ctggttatat gtatacagat ctttcaacta tttatgagag agctggaaga attgagggta gaaatggttc aattactcaa ttcccaattt taacaatgcc aaatgatgat attacccatc caattccaga tcttactggt tacattacag aaggacagat ctttgttgat agagctctct ataacagaca gatttatcca ccaattaatg ttttaccatc attatctcgt cttatgaagt ctggtattgg tactggttta actagagaag atcatccatc agtttctgat caactttatg ctaattatgc tattggccag gatactagag ctatggtagc agttgtagga gaggaagctc tttcagctga tgatttatta tatcttgagt tcactagtaa attcgagtcc aagttccttt ctcaaggtcc atatgagaag agagatatct ttgagagttt ggatcttgct tgggaacttc ttagaacatt ccctgaggat atgcttaaga agattaagca tgatttatta caaaagtatt atccaagaga caattatatt caacattcta agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat