CrhoA.17369.a

vacuolar ATP synthase catalytic subunit A, putative

CENTER ID: CrhoA.17369.a
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.17369.a.B1.GE35447 full length 1 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.70559
RefSeq: XP_665322.1
UniProt: Q5CEQ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCHSWPVRVP RPCLEKLQGN TPLLTGQRIL DALFPSVQGG TCAIPGAFGC GKTCISQALS KYSNSDVIIY IGCGERGNEM AEVLTEFPEL YTMVDGKKES IMQRTCLVAN TSNMPVAARE ASIYTGITLS EYFRDMGCNV SMMADSTSRW AEALREISGR LAEMPADSGY PAYLGARLAS FYERSGRVKC MGSPDREGTV TIVGAVSPPG GDFSDPVTTA TIGIVQVFWG LDKKLAQRKH FPSVNWNTSF SKYSRVLEPY FDSVDPEFNG LRQKISEILQ KENELADILQ LVGKDSLSED QKIVLEVAKI IREDFLQQNA FSDYDFTCPI RKTLGMMKTI GNLYDLSLKA VKDSSQQQQK IGWSTIYNSM RATINKITSM KFLDPRLTDQ EIKNHFSQLN EEVSNQFRAI SDK
NT Sequence
atgtgccatt cttggcctgt tagagttcca agaccatgct tggagaagtt acaaggtaat actcctttat taacaggaca aagaattttg gatgctttgt tcccatcagt acaaggagga acttgtgcta ttccaggagc ttttggttgt ggtaagacat gtatttctca agctttgagt aaatatagta actctgatgt tattatttac attggttgtg gagaaagagg aaatgaaatg gcagaagttc ttacagaatt ccctgagctt tatactatgg ttgatggaaa gaaggagtca attatgcaaa gaacttgttt agtagctaat acatcaaata tgcctgtcgc tgctagagaa gcttccatct acactggtat tacactttct gaatacttta gagatatggg atgtaatgtt tctatgatgg cagattcaac ttctcgttgg gctgaggctc ttagagaaat ttctggtaga ttagctgaaa tgcctgcaga ttcgggttat ccagcatatt taggcgccag acttgcttca ttctatgaaa gatcaggaag agttaaatgt atgggttctc cagatagaga aggtacagta acaattgttg gtgcagtttc tccacctggt ggtgactttt ctgatccagt tactacagct acaataggta ttgttcaagt cttttgggga ttagataaga agcttgctca gagaaaacat ttcccttcag ttaattggaa tacatcattc agtaaatatt caagagtttt ggagccatac tttgattcag ttgatccaga atttaatgga ttaagacaga agatttctga gattttacag aaagagaatg agcttgctga tattttacag cttgttggaa aggattcact ttctgaggat cagaagattg ttctggaggt tgctaagatt attagggaag atttccttca acaaaatgct ttttcagact atgattttac ttgtccaatt cgaaagactc ttggtatgat gaagacaatt ggtaatttat atgatctttc tctaaaagct gttaaggatt catctcaaca acaacagaag attggctggt caacaattta taattcaatg agagctacta ttaataagat cacttcaatg aaattcttgg acccaagatt aactgatcaa gaaatcaaga accacttttc tcagcttaat gaggaggttt caaaccagtt cagagctatt tctgataagt aa
Details for CrhoA.17369.a.B1.GE35447
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMC HSWPVRVPRP CLEKLQGNTP LLTGQRILDA LFPSVQGGTC AIPGAFGCGK TCISQALSKY SNSDVIIYIG CGERGNEMAE VLTEFPELYT MVDGKKESIM QRTCLVANTS NMPVAAREAS IYTGITLSEY FRDMGCNVSM MADSTSRWAE ALREISGRLA EMPADSGYPA YLGARLASFY ERSGRVKCMG SPDREGTVTI VGAVSPPGGD FSDPVTTATI GIVQVFWGLD KKLAQRKHFP SVNWNTSFSK YSRVLEPYFD SVDPEFNGXR QXXSEXLQKE NELAXILQLX GKXSLSEXQX XVLEVAXIIX XDFLQQNAFS XYDFTXPIRX TXXXMXXXXF MIFXXSXXXX SXXXXXXXXX XXXXXXXXXX XSXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatgtgccat tcttggcctg ttagagttcc aagaccatgc ttggagaagt tacaaggtaa tactccttta ttaacaggac aaagaatttt ggatgctttg ttcccatcag tacaaggagg aacttgtgct attccaggag cttttggttg tggtaagaca tgtatttctc aagctttgag taaatatagt aactctgatg ttattattta cattggttgt ggagaaagag gaaatgaaat ggcagaagtt cttacagaat tccctgagct ttatactatg gttgatggaa agaaggagtc aattatgcaa agaacttgtt tagtagctaa tacatcaaat atgcctgtcg ctgctagaga agcttccatc tacactggta ttacactttc tgaatacttt agagatatgg gatgtaatgt ttctatgatg gctgattcaa cttctcgttg ggctgaggct cttagagaaa tttctggtag attagctgaa atgcctgcag attcgggtta tccagcatat ttaggcgcca gacttgcttc attctatgaa agatcaggaa gagttaaatg tatgggttct ccagatagag aaggtacagt aacaattgtt ggtgcagttt ctccacctgg tggtgacttt tctgatccag ttactacagc tacaataggt attgttcaag tcttttgggg attagataag aagcttgctc agagaaaaca tttcccttca gttaattgga atacatcatt cagtaaatat tcaagagttt tggagccata ctttgattca gttgatccag aatttaatgg antaagacag aananttctg agantttaca gaaagagaat gagcttgctg anattttaca gcttgntgga aaggantcac tttctgagna tcagaanant gttctggagg ttgctaanat tattngggan gatttccttc aacaaaatgc tttttcnnac tatgatttta ctngtccaat tcgaangact nntngnanga tgananannn gnnntttatg atctttnnnn aaagcnnnta nnatnnntct cnnnnnnnnn anatngnggn nannnnttnn nnnangnnnn nctnnnntnn nannannnnn tcannnnnnn nnnnnnnnnn nnncnnnnnn nnnnnnnnnn ttnnncnncn tnnnnnnnnn nnnnnnnnnn nnnnnnnncn nnnntnnnnn nnannaa
Expected Protein Sequence
MAHHHHHHMC HSWPVRVPRP CLEKLQGNTP LLTGQRILDA LFPSVQGGTC AIPGAFGCGK TCISQALSKY SNSDVIIYIG CGERGNEMAE VLTEFPELYT MVDGKKESIM QRTCLVANTS NMPVAAREAS IYTGITLSEY FRDMGCNVSM MADSTSRWAE ALREISGRLA EMPADSGYPA YLGARLASFY ERSGRVKCMG SPDREGTVTI VGAVSPPGGD FSDPVTTATI GIVQVFWGLD KKLAQRKHFP SVNWNTSFSK YSRVLEPYFD SVDPEFNGLR QKISEILQKE NELADILQLV GKDSLSEDQK IVLEVAKIIR EDFLQQNAFS DYDFTCPIRK TLGMMKTIGN LYDLSLKAVK DSSQQQQKIG WSTIYNSMRA TINKITSMKF LDPRLTDQEI KNHFSQLNEE VSNQFRAISD K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgcc attcttggcc tgttagagtt ccaagaccat gcttggagaa gttacaaggt aatactcctt tattaacagg acaaagaatt ttggatgctt tgttcccatc agtacaagga ggaacttgtg ctattccagg agcttttggt tgtggtaaga catgtatttc tcaagctttg agtaaatata gtaactctga tgttattatt tacattggtt gtggagaaag aggaaatgaa atggcagaag ttcttacaga attccctgag ctttatacta tggttgatgg aaagaaggag tcaattatgc aaagaacttg tttagtagct aatacatcaa atatgcctgt cgctgctaga gaagcttcca tctacactgg tattacactt tctgaatact ttagagatat gggatgtaat gtttctatga tggcagattc aacttctcgt tgggctgagg ctcttagaga aatttctggt agattagctg aaatgcctgc agattcgggt tatccagcat atttaggcgc cagacttgct tcattctatg aaagatcagg aagagttaaa tgtatgggtt ctccagatag agaaggtaca gtaacaattg ttggtgcagt ttctccacct ggtggtgact tttctgatcc agttactaca gctacaatag gtattgttca agtcttttgg ggattagata agaagcttgc tcagagaaaa catttccctt cagttaattg gaatacatca ttcagtaaat attcaagagt tttggagcca tactttgatt cagttgatcc agaatttaat ggattaagac agaagatttc tgagatttta cagaaagaga atgagcttgc tgatatttta cagcttgttg gaaaggattc actttctgag gatcagaaga ttgttctgga ggttgctaag attattaggg aagatttcct tcaacaaaat gctttttcag actatgattt tacttgtcca attcgaaaga ctcttggtat gatgaagaca attggtaatt tatatgatct ttctctaaaa gctgttaagg attcatctca acaacaacag aagattggct ggtcaacaat ttataattca atgagagcta ctattaataa gatcacttca atgaaattct tggacccaag attaactgat caagaaatca agaaccactt ttctcagctt aatgaggagg tttcaaacca gttcagagct atttctgata agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat