CrhoA.11416.b

nucleotide binding protein, putative

CENTER ID: CrhoA.11416.b
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.11416.b.B1.GE35519 full length 1 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.50297
RefSeq: XP_668480.1
UniProt: Q5CNN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESNTVFYKK PSNETEEIRT KVNKRGLSKT NNTIIPNNNL REGFIPLIYK GVQLISYSYL LNTRSDSNSS SKVSSILRGP IAGSIVTQLI TGTVWDDLDY LVLDFPPGTG DIQLSIAQSI AIDGAIIVTT PQDLSIADVE RGIHLFNKLN IPILTVVENM SYFICDGCEK RHEIFSKGDF SLITEKYGLE PNFNFPLFSN LSKCKFHSNS NEVDFPYVIA AKKNDSVYLE FVKLSEFIAR KLSKNRYSDY KPNFEFDNNR KIVICQIPKD IKEIFVQENT IDPLVKFNVS YIDIRKLCRC AICYDPGKAQ FNETYEPSTL LINQIETMGS YAIMINWSDD HTSIISYNNL IKIFVNKEDE VKLCQNELLT W
NT Sequence
atggaatcaa atacggtatt ttacaaaaaa cctagcaatg agacagaaga aataaggacc aaagtaaaca aaagaggact ttctaagaca aataatacaa taataccaaa taacaatttg agagagggct ttattcctct tatctataaa ggcgtgcaat taattagcta ttcttattta cttaatacga gaagtgattc aaatagttca agtaaggttt caagtatttt gagaggacca attgcaggtt caattgttac acaactaatt actggaactg tttgggacga tcttgattac ttagttcttg atttcccacc aggtacgggc gatattcaac tttcaattgc acaatcaata gcaattgatg gagcgataat agttacaacc cctcaagatt taagtatcgc agatgtggaa agaggaatac atttatttaa taaattgaat atcccaattt taacagttgt tgaaaatatg tcttacttca tctgcgatgg atgcgaaaag agacacgaaa tattttctaa aggtgatttt tccttaataa ctgagaaata tgggctcgag ccaaacttca attttccttt attttcaaac ctttcgaaat gtaaatttca ttccaattca aatgaggttg actttccata tgttattgct gcaaaaaaaa atgacagtgt atatttagag tttgtaaaat tatcagaatt tatagcaaga aaattgtcta agaatagata cagtgactat aagccaaatt ttgaatttga taataataga aagatagtta tctgtcaaat accgaaagat attaaggaaa tttttgtaca agaaaataca attgatccat tggtcaaatt taatgtttca tatattgata ttaggaaatt atgtagatgc gcaatatgtt atgatcctgg gaaggctcaa tttaatgaga catatgaacc atctacatta ttaattaacc aaatcgaaac tatgggctca tatgctatta tgattaattg gtcagacgat cacacaagca tcatttcata taacaatctt ataaaaatat ttgtaaataa agaggacgaa gttaagctat gccaaaatga attgttaact tggtga
Details for CrhoA.11416.b.B1.GE35519
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHHME SNTVFYKKPS NETEEIRTKV NKRGLSKTNN TIIPNNNLRE GFIPLIYKGV QLISYSYLLN TRSDSNSSSK VSSILRGPIA GSIVTQLITG TVWDDLDYLV LDFPPGTGDI QLSIAQSIAI DGAIIVTTPQ DLSIADVERG IHLFNKLNIP ILTVVENMSY FICDGCEKRH EIFSKGDFSL ITEKYGLEPN FNFPLFSNLS KCKFHSNSNE VDFPYVIAAK KNDSVYLEFV KLSXFIARKL SKNRYSDYKP XFEFDNNRKI VICQIPKXIK EIFVQENTID PLXKFNVSYI DIRKLCRCAI CYDPGKXQFX XTYXXXXIIT XSXYGLXCXX XXXXXXXXSF XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX TXXXXXXXXX XXXXXXXXX
Validated NT Sequence
ttttgtttaa ctttaagaag gaganatacc atggctcacc accaccacca ccatatggaa tcaaatacgg tattttacaa aaaacctagc aatgagacag aagaaataag gaccaaagta aacaaaagag gactttctaa gacaaataat acaataatac caaataacaa tttgagagag ggctttattc ctcttatcta taaaggcgtg caattaatta gctattctta tttacttaat acgagaagtg attcaaatag ttcaagtaag gtttcaagta ttttgagagg accaattgca ggttcaattg ttacacaact aattactgga actgtttggg acgatcttga ttacttagtt cttgatttcc caccaggtac gggcgatatt caactttcaa ttgcacaatc aatagcaatt gatggagcga taatagttac aacccctcaa gatttaagta tcgcagatgt ggaaagagga atacatttat ttaataaatt gaatatccca attttaacag ttgttgaaaa tatgtcttac ttcatctgcg atggatgcga aaagagacac gaaatatttt ctaaaggtga tttttcctta ataactgaga aatatgggct cgagccaaac ttcaattttc ctttattttc aaacctttcg aaatgtaaat ttcattccaa ttcaaatgag gttgactttc catatgttat tgctgcaaaa aaaaatgaca gtgtatattt agagtttgta aaattatcan aatttatagc aagaaaattg tctaagaata gatacagtga ctataagcca gantttgaat ttgataataa tagaaagata gttatctgtc aaataccgaa aganattaag gaaatttttg tacaagaaaa tacaattgat ccattggnca aatttaatgt ttcatatatt gatattagga aattatgtag atgcgcaata tgttatgatc ctgggaaggn tcaatttnat ganacntatg nnntcnacnt tattattacc naatcnaant atgggctcnn atgctntntg atnnngncnn annatcnnnn ancatcnttt cnnnnnnnnn ncttnnnaaa nnnttngnnn nnnnnnnnna nnnncnatnn nnnnnannnn nntnnnnnnn nnnnangnnc nnnnnnnnnn acnnnnnnnn nnnnnnnnnn nnannnnnnn nnnnnnnnnn cnnnnnnnna nnnnnnnnng
Expected Protein Sequence
MAHHHHHHME SNTVFYKKPS NETEEIRTKV NKRGLSKTNN TIIPNNNLRE GFIPLIYKGV QLISYSYLLN TRSDSNSSSK VSSILRGPIA GSIVTQLITG TVWDDLDYLV LDFPPGTGDI QLSIAQSIAI DGAIIVTTPQ DLSIADVERG IHLFNKLNIP ILTVVENMSY FICDGCEKRH EIFSKGDFSL ITEKYGLEPN FNFPLFSNLS KCKFHSNSNE VDFPYVIAAK KNDSVYLEFV KLSEFIARKL SKNRYSDYKP NFEFDNNRKI VICQIPKDIK EIFVQENTID PLVKFNVSYI DIRKLCRCAI CYDPGKAQFN ETYEPSTLLI NQIETMGSYA IMINWSDDHT SIISYNNLIK IFVNKEDEVK LCQNELLTW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat caaatacggt attttacaaa aaacctagca atgagacaga agaaataagg accaaagtaa acaaaagagg actttctaag acaaataata caataatacc aaataacaat ttgagagagg gctttattcc tcttatctat aaaggcgtgc aattaattag ctattcttat ttacttaata cgagaagtga ttcaaatagt tcaagtaagg tttcaagtat tttgagagga ccaattgcag gttcaattgt tacacaacta attactggaa ctgtttggga cgatcttgat tacttagttc ttgatttccc accaggtacg ggcgatattc aactttcaat tgcacaatca atagcaattg atggagcgat aatagttaca acccctcaag atttaagtat cgcagatgtg gaaagaggaa tacatttatt taataaattg aatatcccaa ttttaacagt tgttgaaaat atgtcttact tcatctgcga tggatgcgaa aagagacacg aaatattttc taaaggtgat ttttccttaa taactgagaa atatgggctc gagccaaact tcaattttcc tttattttca aacctttcga aatgtaaatt tcattccaat tcaaatgagg ttgactttcc atatgttatt gctgcaaaaa aaaatgacag tgtatattta gagtttgtaa aattatcaga atttatagca agaaaattgt ctaagaatag atacagtgac tataagccaa attttgaatt tgataataat agaaagatag ttatctgtca aataccgaaa gatattaagg aaatttttgt acaagaaaat acaattgatc cattggtcaa atttaatgtt tcatatattg atattaggaa attatgtaga tgcgcaatat gttatgatcc tgggaaggct caatttaatg agacatatga accatctaca ttattaatta accaaatcga aactatgggc tcatatgcta ttatgattaa ttggtcagac gatcacacaa gcatcatttc atataacaat cttataaaaa tatttgtaaa taaagaggac gaagttaagc tatgccaaaa tgaattgtta acttggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t