SSGCID: Target Page

CrhoA.11416.a

nucleotide binding protein, putative

CENTER ID:	CrhoA.11416.a
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.11416.a.B2.GE35518	mature protein		21	355

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:Chro.80366
RefSeq:	XP_666135.1
UniProt:	Q5CH26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIASNRIAI FFGGLISGII FKDYFKTFSR LTINYLKSII FSISYIKTDS VISKYQNHFN NQIDNCVGVD SPDAGIADSC AGCPNAPICA SGQAKKKPIE NIENLSKVKN IILVLSGKGG VGKSTISSQI SWCLSSKKFN VGLLDIDICG PSAPKMMGVQ DNDVHISANG WSPVYVNDNL SVMSTAFLLP QSDDAVIWRG PKKNGLIKQF LSDVVWGELD FLIIDTPPGT SDEHLSIVSY LNGSNVNGAL IVTTPQEIAL QDVRKEINFC KKVGLNILGV VENMGMIFKN AEHDSSVKDM CDNMEVEYLN KIPWDKELLY VCDLGLSICE KFPQSPSSIG IKKLVDIIIY QSKIN

NT Sequence

atgaagatag cctctaatag aattgcaatt ttcttcggtg gactaatatc tggtattata tttaaggatt atttcaagac attttctagg ttaacaataa actatttaaa atctattatt ttttctattt cttatataaa aactgattca gttatttcta aataccaaaa tcattttaat aatcaaattg acaattgcgt aggagttgat tctccagatg caggaatcgc agactcttgt gcaggatgcc caaatgcacc aatatgcgct tctggacaag caaagaaaaa gcctatagag aatattgaaa acttatcaaa agtaaaaaat attattttgg ttctatccgg taaaggaggt gtaggaaaat caactatatc ttcgcaaata tcatggtgtt tatcatcgaa aaaattcaat gttggacttt tagatatcga catttgcggc ccatccgcac caaaaatgat gggagtgcaa gacaatgatg tacacatttc tgcgaatggc tggtctccgg tatatgttaa tgataatctc tcagttatgt ccactgcatt tttacttcct caatctgatg atgctgtaat ttggagaggc ccaaaaaaaa atgggttaat aaaacagttt ctttcagatg tagtttgggg tgaattagac ttcttaatca ttgatactcc tccaggtact tccgacgagc atctctctat agtttcatat ttaaatggtt caaacgtgaa tggagctttg atcgtaacca ctccgcaaga aattgcatta caagatgtta ggaaggaaat taatttttgt aagaaagtgg gtttaaatat ccttggggtt gttgaaaaca tggggatgat ttttaaaaat gctgagcatg attcttcagt taaagatatg tgtgacaata tggaagtaga atatcttaat aaaataccat gggataaaga attactgtac gtatgcgatt tgggtctctc tatctgcgaa aaattccccc agtcgccatc ttcaatcgga attaaaaaat tagttgatat aataatttac caatcaaaaa ttaactaa

Details for CrhoA.11416.a.B2.GE35518

HARVESTED ON:	6/26/2012
SEQUENCED ON:	6/29/2012
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMF KDYFKTFSRL TINYLKSIIF SISYIKTDSV ISKYQNHFNN QIDNCVGVDS PDAGIADSCA GCPNAPICAS GQAKKKPIEN IENLSKVKNI ILVLSGKGGV GKSTISSQIS WCLSSKKFNV GLLDIDICGP SAPKMMGVQD NDVHISANGW SPVYVNDNLS VMSTAFLLPQ SDDAVIWRGP KKNGLIKQFL SDVVWGELDF LIIDTPPGTS DEHLSIVSYL NGSNVNGALI VTTPQEIALQ DVRKEINFCK KVGLNILGVV ENMGMIFKNA EHDSSVKDMC DNMEVEYLNK IPWDKELLYV CDLGLSICEK FPQSPSSIGI KKLVDIIIYQ SKIN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta aggattattt caagacattt tctaggttaa caataaacta tttaaaatct attatttttt ctatttctta tataaaaact gattcagtta tttctaaata ccaaaatcat tttaataatc aaattgacaa ttgcgtagga gttgattctc cagatgcagg aatcgcagac tcttgtgcag gatgcccaaa tgcaccaata tgcgcttctg gacaagcaaa gaaaaagcct atagagaata ttgaaaactt atcaaaagta aaaaatatta ttttggttct atccggtaaa ggaggtgtag gaaaatcaac tatatcttcg caaatatcat ggtgtttatc atcgaaaaaa ttcaatgttg gacttttaga tatcgacatt tgcggcccat ccgcaccaaa aatgatggga gtgcaagaca atgatgtaca catttctgcg aatggctggt ctccggtata tgttaatgat aatctctcag ttatgtccac tgcattttta cttcctcaat ctgatgatgc tgtaatttgg agaggcccaa aaaaaaatgg gttaataaaa cagtttcttt cagatgtagt ttggggtgaa ttagacttct taatcattga tactcctcca ggtacttccg acgagcatct ctctatagtt tcatatttaa atggttcaaa cgtgaatgga gctttgatcg taaccactcc gcaagaaatt gcattacaag atgttaggaa ggaaattaat ttttgtaaga aagtgggttt aaatatcctt ggggttgttg aaaacatggg gatgattttt aaaaatgctg agcatgattc ttcagttaaa gatatgtgtg acaatatgga agtagaatat cttaataaaa taccatggga taaagaatta ctgtacgtat gcgatttggg tctctctatc tgcgaaaaat tcccccagtc gccatcttca atcggaatta aaaaattagt tgatataata atttaccaat caaaaattaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat