SSGCID: Target Page

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
CrhoA.10201.a.B11.GE42428	full length	1	562
CrhoA.10201.a.B2.GE42429	gkyy-keek	13	531
CrhoA.10201.a.B3.GE42430	gkyy-end	13	562
CrhoA.10201.a.B4.GE42431	start-keek	1	531
CrhoA.10201.a.B5.GE42432	ssgk-kaek	11	540
CrhoA.10201.a.B6.GE42433	start-kaek	1	540

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.10201.a.B6.PW38513	start-kaek		1	540

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:CHUDEA8_3460
UniProt:	A0A0S4TKR3

Details for CrhoA.10201.a.B11.GE42428

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	66kDa
OBSERVED MW:	66kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT KINTTYRNSS GKYYYFTTAI NYTNGTPHMG HAYEIVTSDI LVRIARLFGF NTRFQTGTDE HGQKIAATAE KKGISPKELC DENVKQFKNM NEKLQISTDR FIRTTDTDHY ESCKELWRRC EAKGDIYLGK YNGWYNVREE SFMTELEAKM LNYTDPISGL PLTKMEEPSY FFRITNYLES IKNHIIDNPE FIQPESSRES ILARLEALGS NSEDLSISRA SFSWGVPVPN DQEHVMYVWF DALTNYITGL KWADFDNDLE ENSLFNKYWE NTVHIVGKDI TWFHSVIWPA MLLSVGLSLP KTIFAHGFVT APDGKKMSKS LGNVVDPFEQ IDKMGSDPFR YYLAREGRFG NDIKYQPSSV IDYNNGELAD TYGNLISRIT NLTHKFCQGV APSLSQSLLL DKPVDIDKVF EEYYLAWHCF RIDECTQIAM DLSRNVNKFL TDHAPWNKDN GKSDEERIEI IRIVLEGSYY ISILLSPFIT LACKEVFNRL GTPEKIIPEV DINYNNIKPG TPILIGEPLF KRIVNKEEKT FEELKAEKKE REKQRRSGNK KLSETKDKGK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta agataaatac aacatacaga aactcgtcgg gaaaatatta ctatttcacg actgcaataa actatacgaa tggaacacca catatggggc acgcatatga aatagtaact tctgatattc ttgttagaat tgcaaggcta ttcggcttta atacaagatt tcaaacagga actgatgaac atgggcaaaa aattgcagct actgcagaaa aaaaaggcat atctccaaaa gaactttgcg atgaaaatgt aaaacaattt aaaaatatga atgaaaaact tcagattagt acggacaggt ttattagaac aactgatact gatcattatg aatcatgtaa ggagttatgg agacgttgtg aggcgaaagg agacatttac cttggaaagt ataatggatg gtataacgta agagaagaaa gttttatgac tgaattagaa gcaaaaatgc taaattatac tgacccaatt tcgggattac ctttaacaaa gatggaagaa ccttcgtact ttttcagaat aactaattat cttgaatcaa taaaaaacca cattattgac aatccagaat ttatacagcc ggaatcgagt agagaaagta tattagcaag gttagaggct cttggcagca attctgagga cttatctata agtagagcta gtttttcctg gggagttccc gttccaaatg accaggaaca tgttatgtat gtttggtttg atgctctaac aaattatatt actggtttga aatgggctga ttttgataat gatcttgagg aaaattcgtt atttaacaag tattgggaaa atacagttca tatcgttggt aaagatatta cttggttcca ttccgtaatc tggcctgcga tgctattaag tgtcggactt agcttgccaa aaacaatttt tgctcatgga tttgttaccg cacccgacgg taaaaaaatg agcaagagct tggggaatgt tgttgatcca ttcgaacaaa ttgataaaat gggttctgat ccatttagat attacttggc tagagaaggg agatttggga atgatattaa atatcaacct tcttccgtta tagactataa taatggtgaa ttagctgata cttatgggaa tcttatttca agaattacaa atttaactca caaattttgc cagggagttg cgccttcttt atctcaatct ttattgttgg ataaaccagt agatattgat aaagttttcg aagaatatta tttggcttgg cattgtttcc gtattgatga gtgcacgcaa atagcaatgg atttgtcgag gaatgttaat aagttcctta ctgaccacgc accttggaac aaagataatg gcaagtctga tgaagagagg atagaaatta ttagaatcgt attggaaggt tcctactata tttcaattct gcttagtcca tttattactt tggcatgtaa ggaggttttc aatagacttg gaactccaga gaaaataatc ccagaagttg atataaacta taataacatt aaacctggca cgccaatatt gatcggtgaa cctctattta agagaattgt gaataaggaa gaaaagacat tcgaagagct taaagcagag aaaaaagaaa gagagaaaca aagaagatca ggaaataaga aattaagtga aactaaagat aagggaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for CrhoA.10201.a.B2.GE42429

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	61kDa
OBSERVED MW:	61kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHGK YYYFTTAINY TNGTPHMGHA YEIVTSDILV RIARLFGFNT RFQTGTDEHG QKIAATAEKK GISPKELCDE NVKQFKNMNE KLQISTDRFI RTTDTDHYES CKELWRRCEA KGDIYLGKYN GWYNVREESF MTELEAKMLN YTDPISGLPL TKMEEPSYFF RITNYLESIK NHIIDNPEFI QPESSRESIL ARLEALGSNS EDLSISRASF SWGVPVPNDQ EHVMYVWFDA LTNYITGLKW ADFDNDLEEN SLFNKYWENT VHIVGKDITW FHSVIWPAML LSVGLSLPKT IFAHGFVTAP DGKKMSKSLG NVVDPFEQID KMGSDPFRYY LAREGRFGND IKYQPSSVID YNNGELADTY GNLISRITNL THKFCQGVAP SLSQSLLLDK PVDIDKVFEE YYLAWHCFRI DECTQIAMDL SRNVNKFLTD HAPWNKDNGK SDEERIEIIR IVLEGSYYIS ILLSPFITLA CKEVFNRLGT PEKIIPEVDI NYNNIKPGTP ILIGEPLFKR IVNKEEK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggaaaat attactattt cacgactgca ataaactata cgaatggaac accacatatg gggcacgcat atgaaatagt aacttctgat attcttgtta gaattgcaag gctattcggc tttaatacaa gatttcaaac aggaactgat gaacatgggc aaaaaattgc agctactgca gaaaaaaaag gcatatctcc aaaagaactt tgcgatgaaa atgtaaaaca atttaaaaat atgaatgaaa aacttcagat tagtacggac aggtttatta gaacaactga tactgatcat tatgaatcat gtaaggagtt atggagacgt tgtgaggcga aaggagacat ttaccttgga aagtataatg gatggtataa cgtaagagaa gaaagtttta tgactgaatt agaagcaaaa atgctaaatt atactgaccc aatttcggga ttacctttaa caaagatgga agaaccttcg tactttttca gaataactaa ttatcttgaa tcaataaaaa accacattat tgacaatcca gaatttatac agccggaatc gagtagagaa agtatattag caaggttaga ggctcttggc agcaattctg aggacttatc tataagtaga gctagttttt cctggggagt tcccgttcca aatgaccagg aacatgttat gtatgtttgg tttgatgctc taacaaatta tattactggt ttgaaatggg ctgattttga taatgatctt gaggaaaatt cgttatttaa caagtattgg gaaaatacag ttcatatcgt tggtaaagat attacttggt tccattccgt aatctggcct gcgatgctat taagtgtcgg acttagcttg ccaaaaacaa tttttgctca tggatttgtt accgcacccg acggtaaaaa aatgagcaag agcttgggga atgttgttga tccattcgaa caaattgata aaatgggttc tgatccattt agatattact tggctagaga agggagattt gggaatgata ttaaatatca accttcttcc gttatagact ataataatgg tgaattagct gatacttatg ggaatcttat ttcaagaatt acaaatttaa ctcacaaatt ttgccaggga gttgcgcctt ctttatctca atctttattg ttggataaac cagtagatat tgataaagtt ttcgaagaat attatttggc ttggcattgt ttccgtattg atgagtgcac gcaaatagca atggatttgt cgaggaatgt taataagttc cttactgacc acgcaccttg gaacaaagat aatggcaagt ctgatgaaga gaggatagaa attattagaa tcgtattgga aggttcctac tatatttcaa ttctgcttag tccatttatt actttggcat gtaaggaggt tttcaataga cttggaactc cagagaaaat aatcccagaa gttgatataa actataataa cattaaacct ggcacgccaa tattgatcgg tgaacctcta tttaagagaa ttgtgaataa ggaagaaaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for CrhoA.10201.a.B3.GE42430

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHGK YYYFTTAINY TNGTPHMGHA YEIVTSDILV RIARLFGFNT RFQTGTDEHG QKIAATAEKK GISPKELCDE NVKQFKNMNE KLQISTDRFI RTTDTDHYES CKELWRRCEA KGDIYLGKYN GWYNVREESF MTELEAKMLN YTDPISGLPL TKMEEPSYFF RITNYLESIK NHIIDNPEFI QPESSRESIL ARLEALGSNS EDLSISRASF SWGVPVPNDQ EHVMYVWFDA LTNYITGLKW ADFDNDLEEN SLFNKYWENT VHIVGKDITW FHSVIWPAML LSVGLSLPKT IFAHGFVTAP DGKKMSKSLG NVVDPFEQID KMGSDPFRYY LAREGRFGND IKYQPSSVID YNNGELADTY GNLISRITNL THKFCQGVAP SLSQSLLLDK PVDIDKVFEE YYLAWHCFRI DECTQIAMDL SRNVNKFLTD HAPWNKDNGK SDEERIEIIR IVLEGSYYIS ILLSPFITLA CKEVFNRLGT PEKIIPEVDI NYNNIKPGTP ILIGEPLFKR IVNKEEKTFE ELKAEKKERE KQRRSGNKKL SETKDKGK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggaaaat attactattt cacgactgca ataaactata cgaatggaac accacatatg gggcacgcat atgaaatagt aacttctgat attcttgtta gaattgcaag gctattcggc tttaatacaa gatttcaaac aggaactgat gaacatgggc aaaaaattgc agctactgca gaaaaaaaag gcatatctcc aaaagaactt tgcgatgaaa atgtaaaaca atttaaaaat atgaatgaaa aacttcagat tagtacggac aggtttatta gaacaactga tactgatcat tatgaatcat gtaaggagtt atggagacgt tgtgaggcga aaggagacat ttaccttgga aagtataatg gatggtataa cgtaagagaa gaaagtttta tgactgaatt agaagcaaaa atgctaaatt atactgaccc aatttcggga ttacctttaa caaagatgga agaaccttcg tactttttca gaataactaa ttatcttgaa tcaataaaaa accacattat tgacaatcca gaatttatac agccggaatc gagtagagaa agtatattag caaggttaga ggctcttggc agcaattctg aggacttatc tataagtaga gctagttttt cctggggagt tcccgttcca aatgaccagg aacatgttat gtatgtttgg tttgatgctc taacaaatta tattactggt ttgaaatggg ctgattttga taatgatctt gaggaaaatt cgttatttaa caagtattgg gaaaatacag ttcatatcgt tggtaaagat attacttggt tccattccgt aatctggcct gcgatgctat taagtgtcgg acttagcttg ccaaaaacaa tttttgctca tggatttgtt accgcacccg acggtaaaaa aatgagcaag agcttgggga atgttgttga tccattcgaa caaattgata aaatgggttc tgatccattt agatattact tggctagaga agggagattt gggaatgata ttaaatatca accttcttcc gttatagact ataataatgg tgaattagct gatacttatg ggaatcttat ttcaagaatt acaaatttaa ctcacaaatt ttgccaggga gttgcgcctt ctttatctca atctttattg ttggataaac cagtagatat tgataaagtt ttcgaagaat attatttggc ttggcattgt ttccgtattg atgagtgcac gcaaatagca atggatttgt cgaggaatgt taataagttc cttactgacc acgcaccttg gaacaaagat aatggcaagt ctgatgaaga gaggatagaa attattagaa tcgtattgga aggttcctac tatatttcaa ttctgcttag tccatttatt actttggcat gtaaggaggt tttcaataga cttggaactc cagagaaaat aatcccagaa gttgatataa actataataa cattaaacct ggcacgccaa tattgatcgg tgaacctcta tttaagagaa ttgtgaataa ggaagaaaag acattcgaag agcttaaagc agagaaaaaa gaaagagaga aacaaagaag atcaggaaat aagaaattaa gtgaaactaa agataaggga aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for CrhoA.10201.a.B4.GE42431

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	62kDa
OBSERVED MW:	62kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT KINTTYRNSS GKYYYFTTAI NYTNGTPHMG HAYEIVTSDI LVRIARLFGF NTRFQTGTDE HGQKIAATAE KKGISPKELC DENVKQFKNM NEKLQISTDR FIRTTDTDHY ESCKELWRRC EAKGDIYLGK YNGWYNVREE SFMTELEAKM LNYTDPISGL PLTKMEEPSY FFRITNYLES IKNHIIDNPE FIQPESSRES ILARLEALGS NSEDLSISRA SFSWGVPVPN DQEHVMYVWF DALTNYITGL KWADFDNDLE ENSLFNKYWE NTVHIVGKDI TWFHSVIWPA MLLSVGLSLP KTIFAHGFVT APDGKKMSKS LGNVVDPFEQ IDKMGSDPFR YYLAREGRFG NDIKYQPSSV IDYNNGELAD TYGNLISRIT NLTHKFCQGV APSLSQSLLL DKPVDIDKVF EEYYLAWHCF RIDECTQIAM DLSRNVNKFL TDHAPWNKDN GKSDEERIEI IRIVLEGSYY ISILLSPFIT LACKEVFNRL GTPEKIIPEV DINYNNIKPG TPILIGEPLF KRIVNKEEK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta agataaatac aacatacaga aactcgtcgg gaaaatatta ctatttcacg actgcaataa actatacgaa tggaacacca catatggggc acgcatatga aatagtaact tctgatattc ttgttagaat tgcaaggcta ttcggcttta atacaagatt tcaaacagga actgatgaac atgggcaaaa aattgcagct actgcagaaa aaaaaggcat atctccaaaa gaactttgcg atgaaaatgt aaaacaattt aaaaatatga atgaaaaact tcagattagt acggacaggt ttattagaac aactgatact gatcattatg aatcatgtaa ggagttatgg agacgttgtg aggcgaaagg agacatttac cttggaaagt ataatggatg gtataacgta agagaagaaa gttttatgac tgaattagaa gcaaaaatgc taaattatac tgacccaatt tcgggattac ctttaacaaa gatggaagaa ccttcgtact ttttcagaat aactaattat cttgaatcaa taaaaaacca cattattgac aatccagaat ttatacagcc ggaatcgagt agagaaagta tattagcaag gttagaggct cttggcagca attctgagga cttatctata agtagagcta gtttttcctg gggagttccc gttccaaatg accaggaaca tgttatgtat gtttggtttg atgctctaac aaattatatt actggtttga aatgggctga ttttgataat gatcttgagg aaaattcgtt atttaacaag tattgggaaa atacagttca tatcgttggt aaagatatta cttggttcca ttccgtaatc tggcctgcga tgctattaag tgtcggactt agcttgccaa aaacaatttt tgctcatgga tttgttaccg cacccgacgg taaaaaaatg agcaagagct tggggaatgt tgttgatcca ttcgaacaaa ttgataaaat gggttctgat ccatttagat attacttggc tagagaaggg agatttggga atgatattaa atatcaacct tcttccgtta tagactataa taatggtgaa ttagctgata cttatgggaa tcttatttca agaattacaa atttaactca caaattttgc cagggagttg cgccttcttt atctcaatct ttattgttgg ataaaccagt agatattgat aaagttttcg aagaatatta tttggcttgg cattgtttcc gtattgatga gtgcacgcaa atagcaatgg atttgtcgag gaatgttaat aagttcctta ctgaccacgc accttggaac aaagataatg gcaagtctga tgaagagagg atagaaatta ttagaatcgt attggaaggt tcctactata tttcaattct gcttagtcca tttattactt tggcatgtaa ggaggttttc aatagacttg gaactccaga gaaaataatc ccagaagttg atataaacta taataacatt aaacctggca cgccaatatt gatcggtgaa cctctattta agagaattgt gaataaggaa gaaaagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for CrhoA.10201.a.B5.GE42432

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	62kDa
OBSERVED MW:	62kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHSS GKYYYFTTAI NYTNGTPHMG HAYEIVTSDI LVRIARLFGF NTRFQTGTDE HGQKIAATAE KKGISPKELC DENVKQFKNM NEKLQISTDR FIRTTDTDHY ESCKELWRRC EAKGDIYLGK YNGWYNVREE SFMTELEAKM LNYTDPISGL PLTKMEEPSY FFRITNYLES IKNHIIDNPE FIQPESSRES ILARLEALGS NSEDLSISRA SFSWGVPVPN DQEHVMYVWF DALTNYITGL KWADFDNDLE ENSLFNKYWE NTVHIVGKDI TWFHSVIWPA MLLSVGLSLP KTIFAHGFVT APDGKKMSKS LGNVVDPFEQ IDKMGSDPFR YYLAREGRFG NDIKYQPSSV IDYNNGELAD TYGNLISRIT NLTHKFCQGV APSLSQSLLL DKPVDIDKVF EEYYLAWHCF RIDECTQIAM DLSRNVNKFL TDHAPWNKDN GKSDEERIEI IRIVLEGSYY ISILLSPFIT LACKEVFNRL GTPEKIIPEV DINYNNIKPG TPILIGEPLF KRIVNKEEKT FEELKAEK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgtcgg gaaaatatta ctatttcacg actgcaataa actatacgaa tggaacacca catatggggc acgcatatga aatagtaact tctgatattc ttgttagaat tgcaaggcta ttcggcttta atacaagatt tcaaacagga actgatgaac atgggcaaaa aattgcagct actgcagaaa aaaaaggcat atctccaaaa gaactttgcg atgaaaatgt aaaacaattt aaaaatatga atgaaaaact tcagattagt acggacaggt ttattagaac aactgatact gatcattatg aatcatgtaa ggagttatgg agacgttgtg aggcgaaagg agacatttac cttggaaagt ataatggatg gtataacgta agagaagaaa gttttatgac tgaattagaa gcaaaaatgc taaattatac tgacccaatt tcgggattac ctttaacaaa gatggaagaa ccttcgtact ttttcagaat aactaattat cttgaatcaa taaaaaacca cattattgac aatccagaat ttatacagcc ggaatcgagt agagaaagta tattagcaag gttagaggct cttggcagca attctgagga cttatctata agtagagcta gtttttcctg gggagttccc gttccaaatg accaggaaca tgttatgtat gtttggtttg atgctctaac aaattatatt actggtttga aatgggctga ttttgataat gatcttgagg aaaattcgtt atttaacaag tattgggaaa atacagttca tatcgttggt aaagatatta cttggttcca ttccgtaatc tggcctgcga tgctattaag tgtcggactt agcttgccaa aaacaatttt tgctcatgga tttgttaccg cacccgacgg taaaaaaatg agcaagagct tggggaatgt tgttgatcca ttcgaacaaa ttgataaaat gggttctgat ccatttagat attacttggc tagagaaggg agatttggga atgatattaa atatcaacct tcttccgtta tagactataa taatggtgaa ttagctgata cttatgggaa tcttatttca agaattacaa atttaactca caaattttgc cagggagttg cgccttcttt atctcaatct ttattgttgg ataaaccagt agatattgat aaagttttcg aagaatatta tttggcttgg cattgtttcc gtattgatga gtgcacgcaa atagcaatgg atttgtcgag gaatgttaat aagttcctta ctgaccacgc accttggaac aaagataatg gcaagtctga tgaagagagg atagaaatta ttagaatcgt attggaaggt tcctactata tttcaattct gcttagtcca tttattactt tggcatgtaa ggaggttttc aatagacttg gaactccaga gaaaataatc ccagaagttg atataaacta taataacatt aaacctggca cgccaatatt gatcggtgaa cctctattta agagaattgt gaataaggaa gaaaagacat tcgaagagct taaagcagag aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for CrhoA.10201.a.B6.GE42433

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	63kDa
OBSERVED MW:	63kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	43

Validated AA Sequence

MAHHHHHHMT KINTTYRNSS GKYYYFTTAI NYTNGTPHMG HAYEIVTSDI LVRIARLFGF NTRFQTGTDE HGQKIAATAE KKGISPKELC DENVKQFKNM NEKLQISTDR FIRTTDTDHY ESCKELWRRC EAKGDIYLGK YNGWYNVREE SFMTELEAKM LNYTDPISGL PLTKMEEPSY FFRITNYLES IKNHIIDNPE FIQPESSRES ILARLEALGS NSEDLSISRA SFSXXSSRSK

Validated NT Sequence

atgtcttttc ttccttattc acaattctct taaatagagg ttcaccgatc aatattggcg tgccaggttt aatgttatta tagtttatat caacttctgg gattattttc tctggagttc caagtctatt gaaaacctcc ttacatgcca aagtaataaa tggactaagc agaattgaaa tatagtagga accttccaat acgattctaa taatttctat cctctcttca tcagacttgc cattatcttt gttccaaggt gcgtggtcag taaggaactt attaacattc ctcgacaaat ccattgctat ttgcgtgcac tcatcaatac ggaaacaatg ccaagccaaa taatattctt cgaaaacttt atcaatatct actggtttat ccaacaataa agattgagat aaagaaggcg caactccctg gcaaaatttg tgagttaaat ttgtaattct tgaaataaga ttcccataag tatcagctaa ttcaccatta ttatagtcta aaacggaaga aggttgatat ttaatatcat tcccaaatct cccttctcta gccaagtaat atctaaatgg atcagaaccc attttatcaa tttgttcgaa tggatcaaca acattcccca agctcttgct cattttttta ccgtcgggtg cggtaacaaa tccatgagca aaaattgttt ttggcaagct aagtccgaca cttaatagca tcgcaggcca gattacggaa tggaaccaag taatatcttt accaacgata tgaactgtat tttcccaata cttgttaaat aacgaatttt cctcaagatc attatcaaaa tcagcccatt tcaaaccagt aatataattt gttagagcat caaaccaaac atacataaca tgttcctggt catttggaac gggaactnnn nnnggaaaaa ctagctctac ttatagataa gtcctcagaa ttgctgccaa gagcctctaa ccttgctaat atactttctc tactcgattc cggctgtata aattctggat tgtcaataat gtggtttttt attgattcaa gataattagt tattctgaaa aagtacgaag gttcttccat ctttgttaaa ggtaatcccg aaattgggtc agtataattt agcatttttg cttctaattc agtcataaaa ctttcttctc ttacgttata ccatccatta tactttccaa ggtaaatgtc tcctttcgcc tcacaacgtc tccataactc cttacatgat tcataatgat cagtatcagt tgttctaata aacctgtccg tactaatctg aagtttttca ttcatatttt taaattgttt tacattttca tcgcaaagtt cttttggaga tatgcctttt ttttctgcag tagctgcaat tttttgccca tgttcatcag ttcctgtttg aaatcttgta ttaaagccga atagccttgc aattctaaca agaatatcag aagttactat ttcatatgcg tgccccatat gtggtgttcc attcgtatag tttattgcag tcgtgaaata gtaatatttt cccgacgagt ttctgtatgt tgtatttatc ttagtcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMT KINTTYRNSS GKYYYFTTAI NYTNGTPHMG HAYEIVTSDI LVRIARLFGF NTRFQTGTDE HGQKIAATAE KKGISPKELC DENVKQFKNM NEKLQISTDR FIRTTDTDHY ESCKELWRRC EAKGDIYLGK YNGWYNVREE SFMTELEAKM LNYTDPISGL PLTKMEEPSY FFRITNYLES IKNHIIDNPE FIQPESSRES ILARLEALGS NSEDLSISRA SFSWGVPVPN DQEHVMYVWF DALTNYITGL KWADFDNDLE ENSLFNKYWE NTVHIVGKDI TWFHSVIWPA MLLSVGLSLP KTIFAHGFVT APDGKKMSKS LGNVVDPFEQ IDKMGSDPFR YYLAREGRFG NDIKYQPSSV IDYNNGELAD TYGNLISRIT NLTHKFCQGV APSLSQSLLL DKPVDIDKVF EEYYLAWHCF RIDECTQIAM DLSRNVNKFL TDHAPWNKDN GKSDEERIEI IRIVLEGSYY ISILLSPFIT LACKEVFNRL GTPEKIIPEV DINYNNIKPG TPILIGEPLF KRIVNKEEKT FEELKAEK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta agataaatac aacatacaga aactcgtcgg gaaaatatta ctatttcacg actgcaataa actatacgaa tggaacacca catatggggc acgcatatga aatagtaact tctgatattc ttgttagaat tgcaaggcta ttcggcttta atacaagatt tcaaacagga actgatgaac atgggcaaaa aattgcagct actgcagaaa aaaaaggcat atctccaaaa gaactttgcg atgaaaatgt aaaacaattt aaaaatatga atgaaaaact tcagattagt acggacaggt ttattagaac aactgatact gatcattatg aatcatgtaa ggagttatgg agacgttgtg aggcgaaagg agacatttac cttggaaagt ataatggatg gtataacgta agagaagaaa gttttatgac tgaattagaa gcaaaaatgc taaattatac tgacccaatt tcgggattac ctttaacaaa gatggaagaa ccttcgtact ttttcagaat aactaattat cttgaatcaa taaaaaacca cattattgac aatccagaat ttatacagcc ggaatcgagt agagaaagta tattagcaag gttagaggct cttggcagca attctgagga cttatctata agtagagcta gtttttcctg gggagttccc gttccaaatg accaggaaca tgttatgtat gtttggtttg atgctctaac aaattatatt actggtttga aatgggctga ttttgataat gatcttgagg aaaattcgtt atttaacaag tattgggaaa atacagttca tatcgttggt aaagatatta cttggttcca ttccgtaatc tggcctgcga tgctattaag tgtcggactt agcttgccaa aaacaatttt tgctcatgga tttgttaccg cacccgacgg taaaaaaatg agcaagagct tggggaatgt tgttgatcca ttcgaacaaa ttgataaaat gggttctgat ccatttagat attacttggc tagagaaggg agatttggga atgatattaa atatcaacct tcttccgtta tagactataa taatggtgaa ttagctgata cttatgggaa tcttatttca agaattacaa atttaactca caaattttgc cagggagttg cgccttcttt atctcaatct ttattgttgg ataaaccagt agatattgat aaagttttcg aagaatatta tttggcttgg cattgtttcc gtattgatga gtgcacgcaa atagcaatgg atttgtcgag gaatgttaat aagttcctta ctgaccacgc accttggaac aaagataatg gcaagtctga tgaagagagg atagaaatta ttagaatcgt attggaaggt tcctactata tttcaattct gcttagtcca tttattactt tggcatgtaa ggaggttttc aatagacttg gaactccaga gaaaataatc ccagaagttg atataaacta taataacatt aaacctggca cgccaatatt gatcggtgaa cctctattta agagaattgt gaataaggaa gaaaagacat tcgaagagct taaagcagag aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

CENTER ID:	CrhoA.10201.a
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

CrhoA.10201.a

methionyl-tRNA synthetase (MetRS) ortholog; uncharacterized protein

Ordering Clones & Proteins

Clones*

Proteins

External Resources

Sequences

AA Sequence

NT Sequence

PURIFICATION DATe:	7/23/2018
CONCENTRATION:	11.58mg/ml
OBSERVED MW:	63kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	100µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	43