SSGCID: Target Page

CrhoA.01391.a

Ser/Thr protein kinase, putative

CENTER ID:	CrhoA.01391.a
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.01391.a.B1.GE35516	full length		1	669

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:Chro.60584
RefSeq:	XP_667715.1
UniProt:	Q5CLL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYTIHEGCNI SGNTRIYDTP NRHTWDINNG GTDGMRNESS TPRFKHNFNS GYSDKKFLQH GEEMEFTGNN RNDILGRNGN FIRQNISEYK KSIGVMEGSY VDRNMEISET PRVIQQLETN KRQISEEFTE KRDHEYDTRI VDKNTKLSSK IGSQSSLNQT NYKKVNLGGF TEADWYKKIP NWERIIDERY TSVYSSYERL EMIGTGTYAK VWLLKDKITG KKYAGKLLEP HTYPSDTVER IERMFQSEIK NLIVSQCPGV VRLHKIVVGP EGSLLVQDYV DDGTIWRENC CVSELEAFQH FIQLIQSVLY FQDKGVVHRD LKPTNILRYS NKTIVIGDFG WSEQTKKLHL SPSEWPGTLE INPPEVLTFK GPLTEKIDNY AVGMNLLLFL TGRFICRQKG LDVTEAAPYI LKVVELIRSD FNNKILSKGK QKGNTQLKQS QYFMWEIFIG FTDPNPNTRW SIKKALFHPD CIQQLLFCFH NNFPILWHPQ VIAIIKSNII PNNVSSHQTF IEDEKQYCEV GKQSIGMGKS SVANINSSNQ INIHNVSHAT HFNNYSGGQS PVSEDLAESQ RSYYKSVGTS LRPANPPIGH KFFKNDGQQH KLMLNSQFLS GSSDNNSVYN NEFAASLRNQ QQVNTSSAAT LSYTNNGHKF VGVESSVVKQ PHFINNFHY

NT Sequence

atgtatacta ttcacgaagg gtgtaatata tcagggaaca caaggattta tgacactccg aacagacaca cttgggatat caataatgga ggtactgatg gtatgaggaa tgaaagttca acacctagat tcaagcataa ctttaattct ggttattctg ataaaaagtt tctacaacat ggagaagaga tggaatttac agggaacaat agaaatgata tattaggaag aaatggaaac ttcatcagac aaaatatatc agaatataaa aaaagtattg gagtaatgga agggagctat gttgacagaa acatggagat ttcagaaacc ccaagagtaa ttcaacagtt agaaactaat aaacgtcaaa taagtgaaga atttactgag aaaagagatc atgaatatga taccagaatt gtagataaga acactaaatt gagtagcaaa attggatctc aaagctcatt gaaccaaact aactacaaga aggtgaactt gggaggattt actgaggcag attggtataa aaagattcca aattgggaaa gaataattga tgaaagatat acaagcgttt atagttcata tgaaagactt gaaatgatag gaactgggac ttatgctaaa gtttggcttt tgaaagataa aataacaggg aagaaatatg caggaaaact attagaacct catacttatc cctcagatac agtagaaagg attgagagaa tgtttcagtc agaaataaag aatctcatag tgagccaatg cccaggtgta gttaggctac acaagatcgt agtaggtcca gaaggaagcc tattagttca agattatgta gatgatggta ctatttggcg tgagaactgt tgtgtaagcg aattggaagc cttccagcac tttattcaac taattcagag cgtattatac ttccaagaca agggggtagt acacagagac ttgaaaccaa cgaatatttt aagatattca aataaaacaa tagtaatagg tgattttggg tggagtgaac aaacgaagaa gttacatttg agcccctcag aatggccagg aacacttgaa attaatcccc cggaagtatt gacatttaaa gggcctttaa ccgaaaagat agacaactat gcagttggaa tgaacttatt gttgtttctc actggtaggt ttatttgcag acagaaagga ttggatgtta cggaagcagc tccttatatt ctaaaagtag tggaactaat cagaagtgat tttaataaca agattctaag taaaggaaaa caaaaaggga atactcaatt gaagcagagc cagtacttca tgtgggagat ctttattggg tttactgatc ccaacccaaa tacaaggtgg agtattaaga aggctttgtt ccacccagat tgtattcaac agcttttatt ttgtttccat aataactttc caattctatg gcatcctcag gtaattgcta taatcaaatc aaatattata cctaacaatg tgagctctca ccaaactttc atagaagatg agaaacaata ttgtgaagtg ggaaaacaaa gcattggtat gggaaagagt tcagtggcaa acataaatag tagtaatcag ataaatattc ataatgtttc acatgctaca catttcaata attatagtgg cggtcagagt ccagtttctg aagacttagc agaatcccag agaagctatt ataagtcagt tggaacgtct ctaaggcctg caaatcctcc aataggtcat aagttcttta aaaacgacgg acaacaacat aaactaatgc taaacagcca gtttttgtca gggtcttctg ataacaattc agtttataat aatgagtttg ctgcatcatt aagaaaccaa caacaggtga atacctcctc tgcagccact ctaagttata caaacaatgg ccataagttt gttggggttg aaagttctgt tgtgaagcaa ccccatttta tcaataattt tcactattga

Details for CrhoA.01391.a.B1.GE35516

HARVESTED ON:	6/26/2012
SEQUENCED ON:	6/29/2012
EXPECTED MW:	77kDa
OBSERVED MW:	77kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	39

Validated AA Sequence

Validated NT Sequence

attttnttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgtat actattcacg aagggtgtaa tatatcaggg aacacaagga tttatgacac tccgaacaga cacacttggg atatcaataa tggaggtact gatggtatga ggaatgaaag ttcaacacct agattcaagc ataactttaa ttctggttat tctgataaaa agtttctaca acatggagaa gagatggaat ttacagggaa caatagaaat gatatattag gaagaaatgg aaacttcatc agacaaaata tatcagaata taaaaaaagt attggagtaa tggaagggag ctatgttgac agaaacatgg agatttcaga aaccccaaga gtaattcaac agttagaaac taataaacgt caaataagtg aagaatttac tgagaaaaga gatcatgaat atgataccag aattgtagat aagaacacta aattgagtag caaaattgga tctcaaagct cattgaacca aactaactac aagaaggtga acttgggagg atttactgag gcagattggt ataaaaagat tccaaattgg gaaagaataa ttgatgaaag atatacaagc gtttatagtt catatgaaag acttgaaatg ataggaactg ggacttatgc taaagtttgg cttttgaaag ataaaataac agggaagaaa tatgcaggaa aactattaga acctcatact tatccctcag atacngtaga aaggattgag agaatgtttc agtcagaaat aaagaatctc atagtgagcc natgcccagt gtagttaggc tacacaagat cgtagtaggn ccagaaggaa gcctattagt tcaagattat gtagatgang gtactatttg gcgtgagaac tgntgtgtaa gcgaattgga agccttccag cactttattc aactaattnn nagcgnatta tacttccann acnnnggggt agtacncnga gncttgaaan nnacganatt ttanannntc aataaacatn nnannnnnga ttttgggtgn antgancgan gaagttncnt tngancccct cnnannnnng nnnnnnnnna nccccnnnnn nntnnnnntn nnngnnnntn nnnaaannnn nnnantannc nnnnnnnann nnnnnnnnnt nnnnnnnnnn nnnnnnnnnn nnncnannnn nnnnnnnnnn nanncnnnnn nnnnnnnt

Expected Protein Sequence

MAHHHHHHMY TIHEGCNISG NTRIYDTPNR HTWDINNGGT DGMRNESSTP RFKHNFNSGY SDKKFLQHGE EMEFTGNNRN DILGRNGNFI RQNISEYKKS IGVMEGSYVD RNMEISETPR VIQQLETNKR QISEEFTEKR DHEYDTRIVD KNTKLSSKIG SQSSLNQTNY KKVNLGGFTE ADWYKKIPNW ERIIDERYTS VYSSYERLEM IGTGTYAKVW LLKDKITGKK YAGKLLEPHT YPSDTVERIE RMFQSEIKNL IVSQCPGVVR LHKIVVGPEG SLLVQDYVDD GTIWRENCCV SELEAFQHFI QLIQSVLYFQ DKGVVHRDLK PTNILRYSNK TIVIGDFGWS EQTKKLHLSP SEWPGTLEIN PPEVLTFKGP LTEKIDNYAV GMNLLLFLTG RFICRQKGLD VTEAAPYILK VVELIRSDFN NKILSKGKQK GNTQLKQSQY FMWEIFIGFT DPNPNTRWSI KKALFHPDCI QQLLFCFHNN FPILWHPQVI AIIKSNIIPN NVSSHQTFIE DEKQYCEVGK QSIGMGKSSV ANINSSNQIN IHNVSHATHF NNYSGGQSPV SEDLAESQRS YYKSVGTSLR PANPPIGHKF FKNDGQQHKL MLNSQFLSGS SDNNSVYNNE FAASLRNQQQ VNTSSAATLS YTNNGHKFVG VESSVVKQPH FINNFHY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtata ctattcacga agggtgtaat atatcaggga acacaaggat ttatgacact ccgaacagac acacttggga tatcaataat ggaggtactg atggtatgag gaatgaaagt tcaacaccta gattcaagca taactttaat tctggttatt ctgataaaaa gtttctacaa catggagaag agatggaatt tacagggaac aatagaaatg atatattagg aagaaatgga aacttcatca gacaaaatat atcagaatat aaaaaaagta ttggagtaat ggaagggagc tatgttgaca gaaacatgga gatttcagaa accccaagag taattcaaca gttagaaact aataaacgtc aaataagtga agaatttact gagaaaagag atcatgaata tgataccaga attgtagata agaacactaa attgagtagc aaaattggat ctcaaagctc attgaaccaa actaactaca agaaggtgaa cttgggagga tttactgagg cagattggta taaaaagatt ccaaattggg aaagaataat tgatgaaaga tatacaagcg tttatagttc atatgaaaga cttgaaatga taggaactgg gacttatgct aaagtttggc ttttgaaaga taaaataaca gggaagaaat atgcaggaaa actattagaa cctcatactt atccctcaga tacagtagaa aggattgaga gaatgtttca gtcagaaata aagaatctca tagtgagcca atgcccaggt gtagttaggc tacacaagat cgtagtaggt ccagaaggaa gcctattagt tcaagattat gtagatgatg gtactatttg gcgtgagaac tgttgtgtaa gcgaattgga agccttccag cactttattc aactaattca gagcgtatta tacttccaag acaagggggt agtacacaga gacttgaaac caacgaatat tttaagatat tcaaataaaa caatagtaat aggtgatttt gggtggagtg aacaaacgaa gaagttacat ttgagcccct cagaatggcc aggaacactt gaaattaatc ccccggaagt attgacattt aaagggcctt taaccgaaaa gatagacaac tatgcagttg gaatgaactt attgttgttt ctcactggta ggtttatttg cagacagaaa ggattggatg ttacggaagc agctccttat attctaaaag tagtggaact aatcagaagt gattttaata acaagattct aagtaaagga aaacaaaaag ggaatactca attgaagcag agccagtact tcatgtggga gatctttatt gggtttactg atcccaaccc aaatacaagg tggagtatta agaaggcttt gttccaccca gattgtattc aacagctttt attttgtttc cataataact ttccaattct atggcatcct caggtaattg ctataatcaa atcaaatatt atacctaaca atgtgagctc tcaccaaact ttcatagaag atgagaaaca atattgtgaa gtgggaaaac aaagcattgg tatgggaaag agttcagtgg caaacataaa tagtagtaat cagataaata ttcataatgt ttcacatgct acacatttca ataattatag tggcggtcag agtccagttt ctgaagactt agcagaatcc cagagaagct attataagtc agttggaacg tctctaaggc ctgcaaatcc tccaataggt cataagttct ttaaaaacga cggacaacaa cataaactaa tgctaaacag ccagtttttg tcagggtctt ctgataacaa ttcagtttat aataatgagt ttgctgcatc attaagaaac caacaacagg tgaatacctc ctctgcagcc actctaagtt atacaaacaa tggccataag tttgttgggg ttgaaagttc tgttgtgaag caaccccatt ttatcaataa ttttcactat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat