CrhoA.01372.a

phenylalanyl-tRNA synthetase beta chain, putative

CENTER ID: CrhoA.01372.a
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.01372.a.B1.GE35515 full length 1 509
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.30378
RefSeq: XP_668177.1
UniProt: Q5CMW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEVEPVTILE KLESLNCSLS SISISEELKC EHERVIGVIK SLESRNIIKT QFISDNMLSL TDEGKDYLLN GSPEYRLIKI ILEKSDHKIK QDEAMTFLGG SIFKIGLSKC LQKKLIKLNK ELGLIEIPAD SNMDLLNTDE LGKNLFIVGV NGIKESQLTK QDLQIITELK KRKLVVSQKM SYFMINKGED FTVKLKPMIT DLTQEMIMNS SWENNEFKPY NFNAKGKRLP RGNIHPLTRT SRKFKRILSQ MGFEEMPTNR WVESSFWNFD ALFQPQKHPA RDSHDTFFLE TPSTFRNEME LSNDHIDKVK KVHEVGGYGS IGLSYNWSIE EASKNLLRTH TTAVSVRMLY KLAQMYLYNE NGLSFDNFQR KAYFSIDRVF RNESIDATHL AEFHQVEGLI VDKNLTLADL IGTLKTFYEK IGISDLKFKP AFNPYTEPSM EIFGYHTTLK KWIEVGNSGL FRPEMLRPLG FPSDIVVIAW GLSLERPTMI SYNIPNIRDL FSFKAKIFS
NT Sequence
atggaagtgg aacctgtaac aatattagaa aagcttgagt cgctcaattg ttctttaagc agtattagca tttcagaaga actcaaatgt gaacatgaaa gagtaatagg agtaattaaa agtttggagt cccgtaatat tattaaaaca caatttattt cggacaatat gttatctctt acagatgagg gaaaagacta tttgttgaat ggatcacccg aatacagact tattaaaatt attctggaaa agtctgatca taaaattaaa caagacgagg caatgacatt tctgggaggc tccatattta aaatagggct ttcaaaatgt cttcagaaaa agctgatcaa attaaataaa gaattgggac taatagagat tcctgctgat tccaacatgg atttattaaa taccgatgag ttagggaaaa atctttttat tgtgggggta aatggtatta aagagagtca actaactaaa caagatttac aaataataac agaattaaaa aagagaaagc ttgtggtctc acagaaaatg tcttacttca tgattaataa gggggaggac tttactgtga agcttaaacc tatgattact gatttaaccc aagaaatgat tatgaactca agttgggaaa acaacgagtt taaaccctat aattttaatg caaaaggaaa aagactgcca agaggaaata ttcatcctct tactcgcact tcaaggaagt ttaagagaat tttatctcaa atgggatttg aagagatgcc tactaatcgc tgggttgaaa gttcattctg gaattttgat gctcttttcc aacctcaaaa acatcctgca agagattctc atgatacttt cttcttagaa actccaagta ccttcagaaa tgaaatggag ttgtcgaacg atcacattga taaagttaaa aaagtacatg aggttggcgg atacggatca attggtttat cttacaactg gagtattgaa gaagcctcta aaaatcttct tagaactcac acaacagcag tttccgtaag aatgctttat aaacttgctc aaatgtattt atataacgag aacggattat cttttgataa tttccagaga aaggcttact tttcaattga ccgtgttttc agaaatgaat caatcgatgc tacccatctt gctgagtttc atcaggttga gggattaatt gtcgacaaga atcttacact tgcagatcta atcggaacat taaagacttt ctacgaaaag attggtattt ccgatcttaa atttaaacct gcatttaacc cttatactga gccttctatg gaaatctttg gataccacac taccttaaaa aagtggatcg aagttggaaa ttccggctta tttagaccag aaatgcttag accactcgga tttccctctg acattgttgt gattgcatgg ggactttctc ttgaaaggcc aacgatgatt agttataata tcccaaatat acgcgatcta tttagcttta aagcaaaaat attttcataa
Details for CrhoA.01372.a.B1.GE35515
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHME VEPVTILEKL ESLNCSLSSI SISEELKCEH ERVIGVIKSL ESRNIIKTQF ISDNMLSLTD EGKDYLLNGS PEYRLIKIIL EKSDHKIKQD EAMTFLGGSI FKIGLSKCLQ KKLIKLNKEL GLIEIPADSN MDLLNTDELG KNLFIVGVNG IKESQLTKQD LQIITELKKR KLVVSQKMSY FMINKGEDFT VKLKPMITDL TQEMIMNSSW ENNEFKPYNF NAKGKRLPRG NIHPLTRTSR KFKRILSQMG FEEMPTNRWV ESSFWNFDAL FXPXKHPARD SHDTFFLETP STFRNEMELS NDHXDKVKKY XXXXXXINWX ILXWSXXXXL KSXXNXXXXX SXXXXXXAXX XXXXRXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttgttnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatggaagtg gaacctgtaa caatattaga aaagcttgag tcgctcaatt gttctttaag cagtattagc atttcagaag aactcaaatg tgaacatgaa agagtaatag gagtaattaa aagtttggag tcccgtaata ttattaaaac acaatttatt tcggacaata tgttatctct tacagatgag ggaaaagact atttgttgaa tggatcaccc gaatacagac ttattaaaat tattctggaa aagtctgatc ataaaattaa acaagacgag gcaatgacat ttctgggagg ctccatattt aaaatagggc tttcaaaatg tcttcagaaa aagctgatca aattaaataa agaattggga ctaatagaga ttcctgctga ttccaacatg gatttattaa ataccgatga gttagggaaa aatcttttta ttgtgggggt aaatggtatt aaagagagtc aactaactaa acaagattta caaataataa cagaattaaa aaagagaaag cttgtggtct cacagaaaat gtcttacttc atgattaata agggggagga ctttactgtg aagcttaaac ctatgattac tgatttaacc caagaaatga ttatgaactc aagttgggaa aacaacgagt ttaaacccta taattttaat gcaaaaggaa aaagactgcc aagaggaaat attcatcctc ttactcgcac ttcaaggaag tttaagagaa ttttatctca aatgggattt gaagagatgc ctactaatcg ctgggttgaa agttcattct ggaattttga tgctcttttc cnacctcnaa aacatcctgc aagagattct catgatactt tcttcttaga aactccaagt accttcagaa atgaaatgga gttgtcgaac gatcacnttg ataaagttaa aaagtacntg nnnnggngga nanggatcaa ttggttnatc ttannntgga gtantganna ncntctaaaa tcnncntana acnnnnanan annantttcg tanangnttn annaacnngc nnnanngnnt tnnnnnnnna gaannnatnn nnnttnnnna tnnnnannnn nnnnnntttn nnnnnnncnn nnttnnanna nnnnnnnnnn nnnnnnnnnn nnc
Expected Protein Sequence
MAHHHHHHME VEPVTILEKL ESLNCSLSSI SISEELKCEH ERVIGVIKSL ESRNIIKTQF ISDNMLSLTD EGKDYLLNGS PEYRLIKIIL EKSDHKIKQD EAMTFLGGSI FKIGLSKCLQ KKLIKLNKEL GLIEIPADSN MDLLNTDELG KNLFIVGVNG IKESQLTKQD LQIITELKKR KLVVSQKMSY FMINKGEDFT VKLKPMITDL TQEMIMNSSW ENNEFKPYNF NAKGKRLPRG NIHPLTRTSR KFKRILSQMG FEEMPTNRWV ESSFWNFDAL FQPQKHPARD SHDTFFLETP STFRNEMELS NDHIDKVKKV HEVGGYGSIG LSYNWSIEEA SKNLLRTHTT AVSVRMLYKL AQMYLYNENG LSFDNFQRKA YFSIDRVFRN ESIDATHLAE FHQVEGLIVD KNLTLADLIG TLKTFYEKIG ISDLKFKPAF NPYTEPSMEI FGYHTTLKKW IEVGNSGLFR PEMLRPLGFP SDIVVIAWGL SLERPTMISY NIPNIRDLFS FKAKIFS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag tggaacctgt aacaatatta gaaaagcttg agtcgctcaa ttgttcttta agcagtatta gcatttcaga agaactcaaa tgtgaacatg aaagagtaat aggagtaatt aaaagtttgg agtcccgtaa tattattaaa acacaattta tttcggacaa tatgttatct cttacagatg agggaaaaga ctatttgttg aatggatcac ccgaatacag acttattaaa attattctgg aaaagtctga tcataaaatt aaacaagacg aggcaatgac atttctggga ggctccatat ttaaaatagg gctttcaaaa tgtcttcaga aaaagctgat caaattaaat aaagaattgg gactaataga gattcctgct gattccaaca tggatttatt aaataccgat gagttaggga aaaatctttt tattgtgggg gtaaatggta ttaaagagag tcaactaact aaacaagatt tacaaataat aacagaatta aaaaagagaa agcttgtggt ctcacagaaa atgtcttact tcatgattaa taagggggag gactttactg tgaagcttaa acctatgatt actgatttaa cccaagaaat gattatgaac tcaagttggg aaaacaacga gtttaaaccc tataatttta atgcaaaagg aaaaagactg ccaagaggaa atattcatcc tcttactcgc acttcaagga agtttaagag aattttatct caaatgggat ttgaagagat gcctactaat cgctgggttg aaagttcatt ctggaatttt gatgctcttt tccaacctca aaaacatcct gcaagagatt ctcatgatac tttcttctta gaaactccaa gtaccttcag aaatgaaatg gagttgtcga acgatcacat tgataaagtt aaaaaagtac atgaggttgg cggatacgga tcaattggtt tatcttacaa ctggagtatt gaagaagcct ctaaaaatct tcttagaact cacacaacag cagtttccgt aagaatgctt tataaacttg ctcaaatgta tttatataac gagaacggat tatcttttga taatttccag agaaaggctt acttttcaat tgaccgtgtt ttcagaaatg aatcaatcga tgctacccat cttgctgagt ttcatcaggt tgagggatta attgtcgaca agaatcttac acttgcagat ctaatcggaa cattaaagac tttctacgaa aagattggta tttccgatct taaatttaaa cctgcattta acccttatac tgagccttct atggaaatct ttggatacca cactacctta aaaaagtgga tcgaagttgg aaattccggc ttatttagac cagaaatgct tagaccactc ggatttccct ctgacattgt tgtgattgca tggggacttt ctcttgaaag gccaacgatg attagttata atatcccaaa tatacgcgat ctatttagct ttaaagcaaa aatattttca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat