SSGCID: Target Page

CrhoA.01265.a

serine/threonine protein phosphatase, putative

CENTER ID:	CrhoA.01265.a
ORGANISM:	Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrhoA.01265.a.B1.GE35436	full length		1	304

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:Chro.10265
RefSeq:	XP_668132.1
UniProt:	Q5CMT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDLDRQIEQ LRRCEPIKES EVKLLCMKAR EILVEEANVQ RIDTPVTICG DIHGQFFDLM ELFKVGGELP DTNYLFLGDF VDRGYYSVET FLLLIALKVR YPDRIMLIRG NHETRQITQV YGFYDECLRK YGSVNVWRYC TEIFDYLSLA SLIEDRILCV HGGLSPSITT IDEIRSLDRK QEVPHDGSMC DLLWSDPEEI NGWGISPRGA GYIFGSDVVK SFNHCNDIEL ITRAHQLAMD GYKWWFEQNL VTVWSAPNYC YRCGNIATVM ELDEQLNYHF KTFEAAPAEQ RGIPAKRPAP DYFI

NT Sequence

atgagcgatt tagacagaca aattgagcag ttgagacgct gtgagccgat taaagaatct gaagtaaaat tactatgtat gaaggctaga gagattcttg tagaggaggc gaatgtgcaa agaattgata ctccagtgac aatttgcgga gatattcatg gccaattttt tgatctaatg gagcttttta aagtcggtgg tgaacttcct gacacaaatt acttattttt aggtgatttc gttgacagag gatactacag tgtagaaaca ttcttgctcc tcattgctct taaagtcagg tatccagata gaattatgct tatcagagga aaccatgaaa cacgacaaat cacacaagta tatggatttt acgatgaatg tctccgtaag tatgggagtg taaacgtttg gaggtattgt acagaaatat ttgattatct ctctcttgct tcattaattg aagaccgaat tttatgcgtt catggggggt tgtcccctag tattactaca attgatgaaa tacgaagcct agatagaaag caagaagttc ctcatgacgg ttccatgtgc gaccttcttt ggtcagatcc agaggaaata aatggatggg gaataagccc aagaggagca ggatatattt ttgggtcaga tgtagtaaag agtttcaatc attgtaacga tattgaactg attacacgag ctcatcagct tgctatggac ggttataagt ggtggtttga acaaaattta gtcacagttt ggagtgcgcc aaattattgt tatagatgcg gaaatattgc tactgtaatg gagttagatg aacaattaaa ctaccacttt aaaacatttg aggcagctcc tgctgaacaa agaggaattc ctgccaagag acctgctcct gactatttta tttga

Details for CrhoA.01265.a.B1.GE35436

HARVESTED ON:	6/26/2012
SEQUENCED ON:	10/23/2013
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MAHHHHHHMS DLDRQIEQLR RCEPIKESEV KLLCMKAREI LVEEANVQRI DTPVTICGDI HGQFFDLMEL FKVGGELPDT NYLFLGDFVD RGYYSVETFL LLIALKVRYP DRIMLIRGNH ETRQITQVYG FYDECLRKYG SVNVWRYCTE IFDYLSLASL IEDRILCVHG GLSPSITTID EIRSLDRKQE VPHDGSMCDL LWSDPEEING WGISPRGAGY IFGSDVVKSF NHCNDIELIT RAHQLAMDGY KWWFEQNLVT VWSAPNYCYR CGNIATVMEL DEQLNYHFKT F

Validated NT Sequence

ttaagaagga ganataccat ggctcaccac caccaccacc atatgagcga tttagacaga caaattgagc agttgagacg ctgtgagccg attaaagaat ctgaagtaaa attactatgt atgaaggcta gagagattct tgtagaggag gcgaatgtgc aaagaattga tactccagtg acaatttgcg gagatattca tggccaattt tttgatctaa tggagctttt taaagtcggt ggtgaacttc ctgacacaaa ttacttattt ttaggtgatt tcgttgacag aggatactac agtgtagaaa cattcttgct cctcattgct cttaaagtca ggtatccaga tagaattatg cttatcagag gaaaccatga aacacgacaa atcacacaag tatatggatt ttacgatgaa tgtctccgta agtatgggag tgtaaacgtt tggaggtatt gtacagaaat atttgattat ctctctcttg cttcattaat tgaagaccga attttatgcg ttcatggggg gttgtcccct agtattacta caattgatga aatacgaagc ctagatagaa agcaagaagt tcctcatgac ggttccatgt gcgaccttct ttggtcagat ccagaggaaa taaatggatg gggaataagc ccaagaggag caggatatat ttttgggtca gatgtagtaa agagtttcaa tcattgtaac gatattgaac tgattacacg agctcatcag cttgctatgg acggttataa gtggtggttt gaacaaaatt tagtcacagt ttggagtgcg ccaaattatt gttatagatg cggaaatatt gctactgtaa tggagttaga tgaacaatta aactaccact ttaaaacatt tg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg atttagacag acaaattgag cagttgagac gctgtgagcc gattaaagaa tctgaagtaa aattactatg tatgaaggct agagagattc ttgtagagga ggcgaatgtg caaagaattg atactccagt gacaatttgc ggagatattc atggccaatt ttttgatcta atggagcttt ttaaagtcgg tggtgaactt cctgacacaa attacttatt tttaggtgat ttcgttgaca gaggatacta cagtgtagaa acattcttgc tcctcattgc tcttaaagtc aggtatccag atagaattat gcttatcaga ggaaaccatg aaacacgaca aatcacacaa gtatatggat tttacgatga atgtctccgt aagtatggga gtgtaaacgt ttggaggtat tgtacagaaa tatttgatta tctctctctt gcttcattaa ttgaagaccg aattttatgc gttcatgggg ggttgtcccc tagtattact acaattgatg aaatacgaag cctagataga aagcaagaag ttcctcatga cggttccatg tgcgaccttc tttggtcaga tccagaggaa ataaatggat ggggaataag cccaagagga gcaggatata tttttgggtc agatgtagta aagagtttca atcattgtaa cgatattgaa ctgattacac gagctcatca gcttgctatg gacggttata agtggtggtt tgaacaaaat ttagtcacag tttggagtgc gccaaattat tgttatagat gcggaaatat tgctactgta atggagttag atgaacaatt aaactaccac tttaaaacat ttgaggcagc tcctgctgaa caaagaggaa ttcctgccaa gagacctgct cctgactatt ttatttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat