CrhoA.00729.a
S-adenosylmethionine synthetase, putative
| CENTER ID: | CrhoA.00729.a |
| ORGANISM: | Cryptosporidium hominis |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrhoA.00729.a.B1.GE35446 | full length | 1 | 406 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrhoA.00729.a.B1.PW36097 | full length | 1 | 406 | ||
| CrhoA.00729.a.B1.PW37478 | full length | 1 | 406 |
Structures
4ODJ
DEPOSITED: 1/10/2014
DETERMINATION: XRay
CLONE: CrhoA.00729.a.B1.GE35446
PROTEIN: CrhoA.00729.a.B1.PW37315
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | CryptoDB:Chro.70301 |
| OrthoMCL: | OG5_126734 |
| RefSeq: | XP_667488.1 |
| UniProt: | Q5CKX0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSSRLSGNK STYTDLQTTS EQFLFSSESV CSGHPDKLCD QISDAILDAC LEQDPESFVA CETCTKTGFI MVFGEITTKA NVNYERVVRE TVKEIGYDSE EKGLDYKTMD VIIKLEQQSN QIAGCVHVDK NVEDIGAGDQ GMMFGYATNE TKELMPLTHV LATSITRELD YIRMKGVSSR VGWLRPDGKA QVTVEYNCKH GVLIPKRIHT ILVSVQHDEN IENEEIREFV LENVIKKVCP SDLMDKETRI LINPSGRFTI GGPAADAGLT GRKIIVDTYG GWGAHGGGAF SGKDATKVDR SGAYMARLVA KSIVFSGLCS RCLVQVSYGI GIARPLSLYI NTFGTAKDGY NDTKLLEIVN KVFDFRPGIL IKQLNLKSPI FKKTSSGGHF GRSEKEFLWE KPIILQ
NT Sequence
atggattctt cgaggcttag tggaaataaa tccacttaca ctgacttaca aactacttct gagcaatttt tattttcttc agagtcagtt tgtagtggcc acccagataa attatgtgat cagatttcgg atgcaatcct tgatgcgtgc ttggaacaag atccagaaag ctttgtagcg tgtgaaacat gcacaaaaac agggttcatt atggtttttg gtgaaataac tacaaaggct aatgtaaatt acgaaagagt tgtaagagaa acagtgaaag aaattggata tgactctgaa gaaaaagggt tagattacaa aactatggac gtgattatta agctagaaca acaaagtaat caaattgctg ggtgtgtaca tgtagataaa aatgtagaag atattggagc gggtgatcaa ggaatgatgt ttggttatgc tacgaatgaa acaaaagaac tcatgcctct gacgcacgta ttagctacat ctattacaag agagctggat tatatcagaa tgaaaggagt atcttctcgg gtgggttggc tgcgccctga tggaaaggcg caagtgacag tagaatataa ctgcaaacat ggcgtactca ttccaaaaag aattcacact attttagttt cggttcaaca tgatgaaaac atagaaaacg aggaaattag agaatttgtt ctggagaatg taattaaaaa agtatgccct tcagatttga tggacaaaga aactagaata ttaattaatc catctggcag atttacaatt ggggggccag cagcagatgc tggattaaca gggcgcaaga taattgtaga tacatacgga ggatggggtg ctcatggggg tggtgcattt agcgggaaag atgcaactaa agtagataga tcaggtgcat atatggcaag acttgttgca aagtcaatcg tcttttctgg cttgtgtagc agatgtttgg tacaggtttc atatggaatt ggaatagcaa ggcctttatc actatatatt aatacatttg gcacagcgaa agatgggtat aatgacacaa aactactgga gatagttaat aaggtatttg attttaggcc aggaatctta attaagcagc taaatcttaa atctcctatt tttaaaaaga catcaagtgg cggacatttt ggacgatcag aaaaagagtt tctttgggaa aagccaatta ttttacaata g
Details for CrhoA.00729.a.B1.GE35446
| HARVESTED ON: | 6/26/2012 |
| SEQUENCED ON: | 9/14/2016 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMD SSRLSGNKST YTDLQTTSEQ FLFSSESVCS GHPDKLCDQI SDAILDACLE QDPESFVACE TCTKTGFIMV FGEITTKANV NYERVVRETV KEIGYDSEEK GLDYKTMDVI IKLEQQSNQI AGCVHVDKNV EDIGAGDQGM MFGYATNETK ELMPLTHVLA TSITRELDYI RMKGVSSRVG WLRPDGKAQV TVEYNCKHGV LIPKRIHTIL VSVQHDENIE NEEIREFVLE NVIKKVCPSD LMDKETRILI NPSGRFTIGG PAADAGLTGR KIIVDTYGGW GAHGGGAFSG KDATKVDRSG AYMARLVAKS IVFSGLCSRC LVQVSYGIGI ARPLSLYINT FGTAKDGYND TKLLEIVNKV FDFRPGILIK QLNLKSPIFK KTSSGGHFGR SEKEFLWEKP IILQ
Validated NT Sequence
atcttactca cttattgtaa aataattggc ttttcccaaa gaaactcttt ttctgatcgt ccaaaatgtc cgccacttga tgtcttttta aaaataggag atttaagatt tagctgctta attaagattc ctggcctaaa atcaaatacc ttattaacta tctccagtag ttttgtgtca ttatacccat ctttcgctgt gccaaatgta ttaatatata gtgataaagg ccttgctatt ccaattccat atgaaacctg taccaaacat ctgctacaca agccagaaaa gacgattgac tttgcaacaa gtcttgccat atatgcacct gatctatcta ctttagttgc atctttcccg ctaaatgcac cacccccatg agcaccccat cctccgtatg tatctacaat tatcttgcgc cctgttaatc cagcatctgc tgctggaccc ccaattgtaa atctgccaga tggattaatt aatattctag tttctttgtc catcaaatct gaagggcata cttttttaat tacattctcc agaacaaatt ctctaatttc ctcgttttct atgttttcat catgttgaac cgaaactaaa atagtgtgaa ttctttttgg aatgagtacg ccatgtttgc agttatattc tactgtcact tgcgcctttc catcagggcg cagccaaccc acccgagaag atactccttt cattctgata taatccagct ctcttgtaat agatgtagct aatacgtgcg tcagaggcat gagttctttt gtttcattcg tagcataacc aaacatcatt ccttgatcac ccgctccaat atcttctaca tttttatcta catgtacaca cccagcaatt tgattacttt gttgttctag cttaataatc acgtccatag ttttgtaatc taaccctttt tcttcagagt catatccaat ttctttcact gtttctctta caactctttc gtaatttaca ttagcctttg tagttatttc accaaaaacc ataatgaacc ctgtttttgt gcatgtttca cacgctacaa agctttctgg atcttgttcc aagcacgcat caaggattgc atccgaaatc tgatcacata atttatctgg gtggccacta caaactgact ctgaagaaaa taaaaattgc tcagaagtag tttgtaagtc agtgtaagtg gatttatttc cactaagcct cgaagaatcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMD SSRLSGNKST YTDLQTTSEQ FLFSSESVCS GHPDKLCDQI SDAILDACLE QDPESFVACE TCTKTGFIMV FGEITTKANV NYERVVRETV KEIGYDSEEK GLDYKTMDVI IKLEQQSNQI AGCVHVDKNV EDIGAGDQGM MFGYATNETK ELMPLTHVLA TSITRELDYI RMKGVSSRVG WLRPDGKAQV TVEYNCKHGV LIPKRIHTIL VSVQHDENIE NEEIREFVLE NVIKKVCPSD LMDKETRILI NPSGRFTIGG PAADAGLTGR KIIVDTYGGW GAHGGGAFSG KDATKVDRSG AYMARLVAKS IVFSGLCSRC LVQVSYGIGI ARPLSLYINT FGTAKDGYND TKLLEIVNKV FDFRPGILIK QLNLKSPIFK KTSSGGHFGR SEKEFLWEKP IILQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt cttcgaggct tagtggaaat aaatccactt acactgactt acaaactact tctgagcaat ttttattttc ttcagagtca gtttgtagtg gccacccaga taaattatgt gatcagattt cggatgcaat ccttgatgcg tgcttggaac aagatccaga aagctttgta gcgtgtgaaa catgcacaaa aacagggttc attatggttt ttggtgaaat aactacaaag gctaatgtaa attacgaaag agttgtaaga gaaacagtga aagaaattgg atatgactct gaagaaaaag ggttagatta caaaactatg gacgtgatta ttaagctaga acaacaaagt aatcaaattg ctgggtgtgt acatgtagat aaaaatgtag aagatattgg agcgggtgat caaggaatga tgtttggtta tgctacgaat gaaacaaaag aactcatgcc tctgacgcac gtattagcta catctattac aagagagctg gattatatca gaatgaaagg agtatcttct cgggtgggtt ggctgcgccc tgatggaaag gcgcaagtga cagtagaata taactgcaaa catggcgtac tcattccaaa aagaattcac actattttag tttcggttca acatgatgaa aacatagaaa acgaggaaat tagagaattt gttctggaga atgtaattaa aaaagtatgc ccttcagatt tgatggacaa agaaactaga atattaatta atccatctgg cagatttaca attggggggc cagcagcaga tgctggatta acagggcgca agataattgt agatacatac ggaggatggg gtgctcatgg gggtggtgca tttagcggga aagatgcaac taaagtagat agatcaggtg catatatggc aagacttgtt gcaaagtcaa tcgtcttttc tggcttgtgt agcagatgtt tggtacaggt ttcatatgga attggaatag caaggccttt atcactatat attaatacat ttggcacagc gaaagatggg tataatgaca caaaactact ggagatagtt aataaggtat ttgattttag gccaggaatc ttaattaagc agctaaatct taaatctcct atttttaaaa agacatcaag tggcggacat tttggacgat cagaaaaaga gtttctttgg gaaaagccaa ttattttaca atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for CrhoA.00729.a.B1.PW36097
| PURIFICATION DATe: | 8/30/2012 |
| CONCENTRATION: | 9.56mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 19 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD SSRLSGNKST YTDLQTTSEQ FLFSSESVCS GHPDKLCDQI SDAILDACLE QDPESFVACE TCTKTGFIMV FGEITTKANV NYERVVRETV KEIGYDSEEK GLDYKTMDVI IKLEQQSNQI AGCVHVDKNV EDIGAGDQGM MFGYATNETK ELMPLTHVLA TSITRELDYI RMKGVSSRVG WLRPDGKAQV TVEYNCKHGV LIPKRIHTIL VSVQHDENIE NEEIREFVLE NVIKKVCPSD LMDKETRILI NPSGRFTIGG PAADAGLTGR KIIVDTYGGW GAHGGGAFSG KDATKVDRSG AYMARLVAKS IVFSGLCSRC LVQVSYGIGI ARPLSLYINT FGTAKDGYND TKLLEIVNKV FDFRPGILIK QLNLKSPIFK KTSSGGHFGR SEKEFLWEKP IILQ
Validated NT Sequence
atcttactca cttattgtaa aataattggc ttttcccaaa gaaactcttt ttctgatcgt ccaaaatgtc cgccacttga tgtcttttta aaaataggag atttaagatt tagctgctta attaagattc ctggcctaaa atcaaatacc ttattaacta tctccagtag ttttgtgtca ttatacccat ctttcgctgt gccaaatgta ttaatatata gtgataaagg ccttgctatt ccaattccat atgaaacctg taccaaacat ctgctacaca agccagaaaa gacgattgac tttgcaacaa gtcttgccat atatgcacct gatctatcta ctttagttgc atctttcccg ctaaatgcac cacccccatg agcaccccat cctccgtatg tatctacaat tatcttgcgc cctgttaatc cagcatctgc tgctggaccc ccaattgtaa atctgccaga tggattaatt aatattctag tttctttgtc catcaaatct gaagggcata cttttttaat tacattctcc agaacaaatt ctctaatttc ctcgttttct atgttttcat catgttgaac cgaaactaaa atagtgtgaa ttctttttgg aatgagtacg ccatgtttgc agttatattc tactgtcact tgcgcctttc catcagggcg cagccaaccc acccgagaag atactccttt cattctgata taatccagct ctcttgtaat agatgtagct aatacgtgcg tcagaggcat gagttctttt gtttcattcg tagcataacc aaacatcatt ccttgatcac ccgctccaat atcttctaca tttttatcta catgtacaca cccagcaatt tgattacttt gttgttctag cttaataatc acgtccatag ttttgtaatc taaccctttt tcttcagagt catatccaat ttctttcact gtttctctta caactctttc gtaatttaca ttagcctttg tagttatttc accaaaaacc ataatgaacc ctgtttttgt gcatgtttca cacgctacaa agctttctgg atcttgttcc aagcacgcat caaggattgc atccgaaatc tgatcacata atttatctgg gtggccacta caaactgact ctgaagaaaa taaaaattgc tcagaagtag tttgtaagtc agtgtaagtg gatttatttc cactaagcct cgaagaatcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMD SSRLSGNKST YTDLQTTSEQ FLFSSESVCS GHPDKLCDQI SDAILDACLE QDPESFVACE TCTKTGFIMV FGEITTKANV NYERVVRETV KEIGYDSEEK GLDYKTMDVI IKLEQQSNQI AGCVHVDKNV EDIGAGDQGM MFGYATNETK ELMPLTHVLA TSITRELDYI RMKGVSSRVG WLRPDGKAQV TVEYNCKHGV LIPKRIHTIL VSVQHDENIE NEEIREFVLE NVIKKVCPSD LMDKETRILI NPSGRFTIGG PAADAGLTGR KIIVDTYGGW GAHGGGAFSG KDATKVDRSG AYMARLVAKS IVFSGLCSRC LVQVSYGIGI ARPLSLYINT FGTAKDGYND TKLLEIVNKV FDFRPGILIK QLNLKSPIFK KTSSGGHFGR SEKEFLWEKP IILQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATT CTTCGAGGCT TAGTGGAAAT AAATCCACTT ACACTGACTT ACAAACTACT TCTGAGCAAT TTTTATTTTC TTCAGAGTCA GTTTGTAGTG GCCACCCAGA TAAATTATGT GATCAGATTT CGGATGCAAT CCTTGATGCG TGCTTGGAAC AAGATCCAGA AAGCTTTGTA GCGTGTGAAA CATGCACAAA AACAGGGTTC ATTATGGTTT TTGGTGAAAT AACTACAAAG GCTAATGTAA ATTACGAAAG AGTTGTAAGA GAAACAGTGA AAGAAATTGG ATATGACTCT GAAGAAAAAG GGTTAGATTA CAAAACTATG GACGTGATTA TTAAGCTAGA ACAACAAAGT AATCAAATTG CTGGGTGTGT ACATGTAGAT AAAAATGTAG AAGATATTGG AGCGGGTGAT CAAGGAATGA TGTTTGGTTA TGCTACGAAT GAAACAAAAG AACTCATGCC TCTGACGCAC GTATTAGCTA CATCTATTAC AAGAGAGCTG GATTATATCA GAATGAAAGG AGTATCTTCT CGGGTGGGTT GGCTGCGCCC TGATGGAAAG GCGCAAGTGA CAGTAGAATA TAACTGCAAA CATGGCGTAC TCATTCCAAA AAGAATTCAC ACTATTTTAG TTTCGGTTCA ACATGATGAA AACATAGAAA ACGAGGAAAT TAGAGAATTT GTTCTGGAGA ATGTAATTAA AAAAGTATGC CCTTCAGATT TGATGGACAA AGAAACTAGA ATATTAATTA ATCCATCTGG CAGATTTACA ATTGGGGGGC CAGCAGCAGA TGCTGGATTA ACAGGGCGCA AGATAATTGT AGATACATAC GGAGGATGGG GTGCTCATGG GGGTGGTGCA TTTAGCGGGA AAGATGCAAC TAAAGTAGAT AGATCAGGTG CATATATGGC AAGACTTGTT GCAAAGTCAA TCGTCTTTTC TGGCTTGTGT AGCAGATGTT TGGTACAGGT TTCATATGGA ATTGGAATAG CAAGGCCTTT ATCACTATAT ATTAATACAT TTGGCACAGC GAAAGATGGG TATAATGACA CAAAACTACT GGAGATAGTT AATAAGGTAT TTGATTTTAG GCCAGGAATC TTAATTAAGC AGCTAAATCT TAAATCTCCT ATTTTTAAAA AGACATCAAG TGGCGGACAT TTTGGACGAT CAGAAAAAGA GTTTCTTTGG GAAAAGCCAA TTATTTTACA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
Details for CrhoA.00729.a.B1.PW37478
| PURIFICATION DATe: | 4/9/2014 |
| CONCENTRATION: | 10mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD SSRLSGNKST YTDLQTTSEQ FLFSSESVCS GHPDKLCDQI SDAILDACLE QDPESFVACE TCTKTGFIMV FGEITTKANV NYERVVRETV KEIGYDSEEK GLDYKTMDVI IKLEQQSNQI AGCVHVDKNV EDIGAGDQGM MFGYATNETK ELMPLTHVLA TSITRELDYI RMKGVSSRVG WLRPDGKAQV TVEYNCKHGV LIPKRIHTIL VSVQHDENIE NEEIREFVLE NVIKKVCPSD LMDKETRILI NPSGRFTIGG PAADAGLTGR KIIVDTYGGW GAHGGGAFSG KDATKVDRSG AYMARLVAKS IVFSGLCSRC LVQVSYGIGI ARPLSLYINT FGTAKDGYND TKLLEIVNKV FDFRPGILIK QLNLKSPIFK KTSSGGHFGR SEKEFLWEKP IILQ
Validated NT Sequence
atcttactca cttattgtaa aataattggc ttttcccaaa gaaactcttt ttctgatcgt ccaaaatgtc cgccacttga tgtcttttta aaaataggag atttaagatt tagctgctta attaagattc ctggcctaaa atcaaatacc ttattaacta tctccagtag ttttgtgtca ttatacccat ctttcgctgt gccaaatgta ttaatatata gtgataaagg ccttgctatt ccaattccat atgaaacctg taccaaacat ctgctacaca agccagaaaa gacgattgac tttgcaacaa gtcttgccat atatgcacct gatctatcta ctttagttgc atctttcccg ctaaatgcac cacccccatg agcaccccat cctccgtatg tatctacaat tatcttgcgc cctgttaatc cagcatctgc tgctggaccc ccaattgtaa atctgccaga tggattaatt aatattctag tttctttgtc catcaaatct gaagggcata cttttttaat tacattctcc agaacaaatt ctctaatttc ctcgttttct atgttttcat catgttgaac cgaaactaaa atagtgtgaa ttctttttgg aatgagtacg ccatgtttgc agttatattc tactgtcact tgcgcctttc catcagggcg cagccaaccc acccgagaag atactccttt cattctgata taatccagct ctcttgtaat agatgtagct aatacgtgcg tcagaggcat gagttctttt gtttcattcg tagcataacc aaacatcatt ccttgatcac ccgctccaat atcttctaca tttttatcta catgtacaca cccagcaatt tgattacttt gttgttctag cttaataatc acgtccatag ttttgtaatc taaccctttt tcttcagagt catatccaat ttctttcact gtttctctta caactctttc gtaatttaca ttagcctttg tagttatttc accaaaaacc ataatgaacc ctgtttttgt gcatgtttca cacgctacaa agctttctgg atcttgttcc aagcacgcat caaggattgc atccgaaatc tgatcacata atttatctgg gtggccacta caaactgact ctgaagaaaa taaaaattgc tcagaagtag tttgtaagtc agtgtaagtg gatttatttc cactaagcct cgaagaatcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMD SSRLSGNKST YTDLQTTSEQ FLFSSESVCS GHPDKLCDQI SDAILDACLE QDPESFVACE TCTKTGFIMV FGEITTKANV NYERVVRETV KEIGYDSEEK GLDYKTMDVI IKLEQQSNQI AGCVHVDKNV EDIGAGDQGM MFGYATNETK ELMPLTHVLA TSITRELDYI RMKGVSSRVG WLRPDGKAQV TVEYNCKHGV LIPKRIHTIL VSVQHDENIE NEEIREFVLE NVIKKVCPSD LMDKETRILI NPSGRFTIGG PAADAGLTGR KIIVDTYGGW GAHGGGAFSG KDATKVDRSG AYMARLVAKS IVFSGLCSRC LVQVSYGIGI ARPLSLYINT FGTAKDGYND TKLLEIVNKV FDFRPGILIK QLNLKSPIFK KTSSGGHFGR SEKEFLWEKP IILQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATT CTTCGAGGCT TAGTGGAAAT AAATCCACTT ACACTGACTT ACAAACTACT TCTGAGCAAT TTTTATTTTC TTCAGAGTCA GTTTGTAGTG GCCACCCAGA TAAATTATGT GATCAGATTT CGGATGCAAT CCTTGATGCG TGCTTGGAAC AAGATCCAGA AAGCTTTGTA GCGTGTGAAA CATGCACAAA AACAGGGTTC ATTATGGTTT TTGGTGAAAT AACTACAAAG GCTAATGTAA ATTACGAAAG AGTTGTAAGA GAAACAGTGA AAGAAATTGG ATATGACTCT GAAGAAAAAG GGTTAGATTA CAAAACTATG GACGTGATTA TTAAGCTAGA ACAACAAAGT AATCAAATTG CTGGGTGTGT ACATGTAGAT AAAAATGTAG AAGATATTGG AGCGGGTGAT CAAGGAATGA TGTTTGGTTA TGCTACGAAT GAAACAAAAG AACTCATGCC TCTGACGCAC GTATTAGCTA CATCTATTAC AAGAGAGCTG GATTATATCA GAATGAAAGG AGTATCTTCT CGGGTGGGTT GGCTGCGCCC TGATGGAAAG GCGCAAGTGA CAGTAGAATA TAACTGCAAA CATGGCGTAC TCATTCCAAA AAGAATTCAC ACTATTTTAG TTTCGGTTCA ACATGATGAA AACATAGAAA ACGAGGAAAT TAGAGAATTT GTTCTGGAGA ATGTAATTAA AAAAGTATGC CCTTCAGATT TGATGGACAA AGAAACTAGA ATATTAATTA ATCCATCTGG CAGATTTACA ATTGGGGGGC CAGCAGCAGA TGCTGGATTA ACAGGGCGCA AGATAATTGT AGATACATAC GGAGGATGGG GTGCTCATGG GGGTGGTGCA TTTAGCGGGA AAGATGCAAC TAAAGTAGAT AGATCAGGTG CATATATGGC AAGACTTGTT GCAAAGTCAA TCGTCTTTTC TGGCTTGTGT AGCAGATGTT TGGTACAGGT TTCATATGGA ATTGGAATAG CAAGGCCTTT ATCACTATAT ATTAATACAT TTGGCACAGC GAAAGATGGG TATAATGACA CAAAACTACT GGAGATAGTT AATAAGGTAT TTGATTTTAG GCCAGGAATC TTAATTAAGC AGCTAAATCT TAAATCTCCT ATTTTTAAAA AGACATCAAG TGGCGGACAT TTTGGACGAT CAGAAAAAGA GTTTCTTTGG GAAAAGCCAA TTATTTTACA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT