CrhoA.00164.a

arginyl-tRNA synthetase, putative

CENTER ID: CrhoA.00164.a
ORGANISM: Cryptosporidium hominis
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrhoA.00164.a.B1.GE35437 full length 1 505
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:Chro.50402
RefSeq: XP_665270.1
UniProt: Q5CEK7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDEIKVTPTM KFHCKSGRKT PKVIVDFSSP NIAKEMHVGH LRSTIIGDTT SRILEYLGFE VLRVNHVGDW GTQFGMLIEY LKEEYPTFVE NLPEISDLQE FYKNSKKRFD TDPQFKLRSQ QNVVKLQGGD QDALIAWKKI CEVSRVEFQK IYDRLGVRLE EFGESYYNDM IPGVVKMLDE KGILKLDNGA QCVYTKINEI PLMAVKSDGG YGYDSTDLAA IHHRLITLDA DWVVYTTDMG QEEHFMKLFD VAETMGWHDP SNTRLFFIGF GVIQGEDGKK FKTRSGDVVK LTELIDEGVA RAKKELVDRR NQRRSDQTIK SNTDVEVDHP DIDDSNLDLM ANTIGHAAIK YFDLKQNRLT NYRFSFDRML DPKGNTAVYL LYAYARICAI FRKVEFIQSD DNMVIKQKVS NFKLKISNNV ERKLSIQLLR FPEVFDGILN DFFPNRLADY CYELSESFAQ FYTECRIIGL EEQQLEQSRL LLCFLTKQIL LKGLDLLGVE TLEKI
NT Sequence
atggatgaga taaaagtaac cccaacaatg aaattccact gcaaaagtgg aagaaaaact ccaaaagtaa ttgtagattt ctcatcacca aatattgcca aagaaatgca tgtgggacac ttaagatcta caatcattgg tgatacaaca tctagaattc ttgaatattt aggatttgaa gtattaagag ttaatcatgt tggagattgg ggtactcaat ttggtatgct tattgaatat ttaaaggaag aatatcccac ttttgttgaa aatttaccag aaatatcaga tttacaagaa ttctataaga actcaaagaa aaggtttgat actgatccac aatttaagtt aaggagccaa cagaatgttg ttaagttaca agggggtgat caagatgctt tgattgcttg gaaaaagatt tgtgaggtta gccgagttga atttcagaaa atttacgatc gacttggagt tcgtcttgag gaatttggtg aaagttatta taatgatatg atccccgggg tggttaaaat gttggatgaa aaaggtattc ttaagcttga taatggagct cagtgtgttt atactaaaat taatgaaata cctttaatgg ctgttaaaag tgatggagga tatggatatg attcaacaga tttagctgca attcaccata gattaatcac tctagatgct gattgggttg tctacactac tgatatgggc caagaagaac attttatgaa actttttgac gttgctgaaa ctatgggatg gcatgatcct tctaatacaa gactattttt cattggattt ggagttattc aaggagaaga tggaaaaaag tttaaaacaa gaagtggtga tgttgtcaaa ttaactgaac ttattgacga aggtgttgct agagcaaaga aagaattagt tgacagaaga aatcaaagaa gatctgacca aacaattaaa tcaaatacag atgttgaagt tgatcatcca gatattgatg attctaactt ggatttaatg gcaaatacta ttggccatgc agctattaaa tactttgatt tgaagcaaaa tagacttact aattacagat tttcatttga tcgaatgtta gatccaaaag gtaatactgc agtttattta ctatatgcat atgcaagaat ttgtgcaatc tttagaaaag ttgaatttat tcaatctgat gataatatgg taattaaaca gaaagtttca aactttaagc ttaagatttc taataatgtt gaacgaaaat tgtctattca gttactcaga ttccctgaag tttttgatgg aattttgaat gatttcttcc ccaatagact tgctgattat tgttatgaat tgtccgagag ttttgcacaa ttttatactg agtgcagaat tattggatta gaagaacagc aacttgaaca aagtaggctt ttactttgtt tcttaacaaa acaaattctt ttaaaaggac ttgatttatt gggagttgaa actttggaga agatttaa
Details for CrhoA.00164.a.B1.GE35437
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 59kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 57
Validated AA Sequence
MAHHHHHHMD EIKVTPTMKF HCKIGRKTPK VIVDFSSPNI AKEMHVGHLR STIIGDTTSR ILEYLGFEVL RVNHVGDWGT QFGMLIEYLK EEYPTFVENL PEISDLQEFY KNSKKRFDTD PQFKLRSQQN VVKLQGGDQD ALIAWKKICE VSRVEFQKIY DRLGVRLEEF GESYYNDMIP GVVKMLDEKX ILKLDNGAQC VYTKINEIPL MAVKSDGXXG YXSTDLAAIH HRLITLDADW VVYTTDMGXE XHXXKLLTXL XLXXGMILXX QDXFXXXGXX XXXXXXXXGX XXXX
Validated NT Sequence
tttgtttact ttaagaagga ganataccat ggctcaccac caccaccacc atatggatga gataaaagta accccaacaa tgaaattcca ctgcaaaatt ggaagaaaaa ctccaaaagt aattgtagat ttctcatcac caaatattgc caaagaaatg catgtgggac acttaagatc tacaatcatt ggtgatacaa catctagaat tcttgaatat ttaggatttg aagtattaag agttaatcat gttggagatt ggggtactca atttggtatg cttattgaat atttaaagga agaatatccc acttttgttg aaaatttacc agaaatatca gatttacaag aattctataa gaactcaaag aaaaggtttg atactgatcc acaatttaag ttaaggagcc aacagaatgt tgttaagtta caagggggtg atcaagatgc tttgattgct tggaaaaaga tttgtgaggt tagccgagtt gaatttcaga aaatttacga tcgacttgga gttcgtcttg aggaatttgg tgaaagttat tataatgata tgatccccgg ggtggttaaa atgttggatg aaaaangtat tcttaagctt gataatggag ctcagtgtgt ttatactaaa attaatgaaa tacctttaat ggctgttaaa agtgatggag nanatggata tgantcaaca gatttagctg caattcacca tagattaatc actctagatg ctgattgggt ngtctacact actgatatgg gccnagaana acatttnntg aaacttttga cgtngctnaa nctangggnn ggcatgatcc tncnannnca agacnntttt nntngnnttg gantnnntcn nnaaannttn naancnnnna nnggngnnnn nnnnaannan tgantnntnc nannnnnncn nnnnannnan nnnnnnnnnn nnnnaatcna gnnnannnnn nnaannnnnn nnnnnnnnnn nnnnantnnn nnnnnntnnn ggnaannnnn nnncnnnnnn nnnannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncn tnnnnnnnnt tnnngnnnan g
Expected Protein Sequence
MAHHHHHHMD EIKVTPTMKF HCKSGRKTPK VIVDFSSPNI AKEMHVGHLR STIIGDTTSR ILEYLGFEVL RVNHVGDWGT QFGMLIEYLK EEYPTFVENL PEISDLQEFY KNSKKRFDTD PQFKLRSQQN VVKLQGGDQD ALIAWKKICE VSRVEFQKIY DRLGVRLEEF GESYYNDMIP GVVKMLDEKG ILKLDNGAQC VYTKINEIPL MAVKSDGGYG YDSTDLAAIH HRLITLDADW VVYTTDMGQE EHFMKLFDVA ETMGWHDPSN TRLFFIGFGV IQGEDGKKFK TRSGDVVKLT ELIDEGVARA KKELVDRRNQ RRSDQTIKSN TDVEVDHPDI DDSNLDLMAN TIGHAAIKYF DLKQNRLTNY RFSFDRMLDP KGNTAVYLLY AYARICAIFR KVEFIQSDDN MVIKQKVSNF KLKISNNVER KLSIQLLRFP EVFDGILNDF FPNRLADYCY ELSESFAQFY TECRIIGLEE QQLEQSRLLL CFLTKQILLK GLDLLGVETL EKI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatg agataaaagt aaccccaaca atgaaattcc actgcaaaag tggaagaaaa actccaaaag taattgtaga tttctcatca ccaaatattg ccaaagaaat gcatgtggga cacttaagat ctacaatcat tggtgataca acatctagaa ttcttgaata tttaggattt gaagtattaa gagttaatca tgttggagat tggggtactc aatttggtat gcttattgaa tatttaaagg aagaatatcc cacttttgtt gaaaatttac cagaaatatc agatttacaa gaattctata agaactcaaa gaaaaggttt gatactgatc cacaatttaa gttaaggagc caacagaatg ttgttaagtt acaagggggt gatcaagatg ctttgattgc ttggaaaaag atttgtgagg ttagccgagt tgaatttcag aaaatttacg atcgacttgg agttcgtctt gaggaatttg gtgaaagtta ttataatgat atgatccccg gggtggttaa aatgttggat gaaaaaggta ttcttaagct tgataatgga gctcagtgtg tttatactaa aattaatgaa atacctttaa tggctgttaa aagtgatgga ggatatggat atgattcaac agatttagct gcaattcacc atagattaat cactctagat gctgattggg ttgtctacac tactgatatg ggccaagaag aacattttat gaaacttttt gacgttgctg aaactatggg atggcatgat ccttctaata caagactatt tttcattgga tttggagtta ttcaaggaga agatggaaaa aagtttaaaa caagaagtgg tgatgttgtc aaattaactg aacttattga cgaaggtgtt gctagagcaa agaaagaatt agttgacaga agaaatcaaa gaagatctga ccaaacaatt aaatcaaata cagatgttga agttgatcat ccagatattg atgattctaa cttggattta atggcaaata ctattggcca tgcagctatt aaatactttg atttgaagca aaatagactt actaattaca gattttcatt tgatcgaatg ttagatccaa aaggtaatac tgcagtttat ttactatatg catatgcaag aatttgtgca atctttagaa aagttgaatt tattcaatct gatgataata tggtaattaa acagaaagtt tcaaacttta agcttaagat ttctaataat gttgaacgaa aattgtctat tcagttactc agattccctg aagtttttga tggaattttg aatgatttct tccccaatag acttgctgat tattgttatg aattgtccga gagttttgca caattttata ctgagtgcag aattattgga ttagaagaac agcaacttga acaaagtagg cttttacttt gtttcttaac aaaacaaatt cttttaaaag gacttgattt attgggagtt gaaactttgg agaagatttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat