CrfaA.20383.a

mitogen-activated protein kinase kinase 2

CENTER ID: CrfaA.20383.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.20383.a.B2.GE43782 kinase domain 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:CFAC1_220032700

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEDYQIIESI GEGSFGKVYK ARIKGTGQIV AMKFIVKKGK NEKELKNLRS EIEILTKLNH PHIIMLFDSF ETDSDFVVVM EYAQGELYDI LEDDKQLPAK EVQKIAKQLI QALNYLHSNR IIHRDMKPQN ILIGQNGAVK LADFGFARSM SYNTIVLTSI KGTPLYMAPE LVQERAYDNR VDLWSLGCIL YELYYGKPPF YTNNLFALIK KIVCEPVKFD NKADDPISPE FKNFLSGLLT KSASSRLNWP DLLNHPFVQL TKGDASWQDA IMRHDSKMKG RMDKLGCLRL HGGASRAAPK HQVAQKDEII NAQTAYVLEA GRESEVRLTL GQLVGAVNAA SGDLSKCSVL LGIFDFGVLE ATLKLFAPKQ PRTTVQLILN LLQALLFPEG GDVVSFPSQR PKRDGVDVLV EKNSHQQVDL FVRQQVAIGL TKKPHDALDY IIGEIGSNSN AQAEVCVKIV FQCFRWENSF GPTVTQLRAF PEMWSAVLSS VDHDSVSKGK VSYEYAALVF HTVSIVIPQI KLAAPQRINR EKVLHLVTQA LSAVCYYEVS AIDAQRNKIP PLTYAAAAAL LIAFAHRELK DIVSFTVNDA LLDGIYSIVD AVSATPSRPI TPRAVGSSYG YPDYGLLDGV AHMLSLIFSD PDSLVYAKLP GKAEHRFLES DTKNLALLVI ALLRDSDSRT ELSPNGVQTL LRAAQQIFQQ QKEQTASMSL LMDTVPPYAG ESGNPCWLAV VCRTLKTEYF KQLFNWPACR GGGATGVSAH VTIVSQILAD SLRPASSSSN PTAAEEKLLS DVSRVLFKER VVELLVNAMD FSEGVFLGSP FSIVAKLCAN SADFIRAFVD CGGVESARIR RVLDPDKAGT GLMSDGLVVL SQMARMSAEF YESIHKANLY DCIAALLQHS EKDLRGKTCT LIGNLCKHSD YFFQPLERNH IVERLVKCCS DSDAQTQKLA AFAIGNAAFH SDYLYRLLTP AIPSLVGLLA CGDAKTRQNA AGALSNFVRN GDQLVGALEA SKVVEALLKM LRDDDSLSSK KVAVLTINSF CTYDVFRRKF VELDLREDIR KLQQDSTARG DPSIQKYVGK LIDRLG
NT Sequence
ATGGAGGACT ATCAAATTAT AGAGTCCATT GGGGAGGGCT CCTTTGGCAA GGTTTACAAG GCACGCATCA AGGGAACGGG TCAGATTGTA GCGATGAAAT TCATTGTTAA GAAGGGAAAG AATGAGAAGG AGCTGAAGAA CCTGCGCAGT GAGATCGAGA TTCTTACCAA ACTGAATCAC CCCCACATCA TCATGCTCTT TGACTCATTT GAGACAGACT CGGACTTTGT TGTCGTGATG GAGTATGCGC AGGGTGAGTT GTACGACATT CTAGAAGACG ACAAACAGCT GCCGGCTAAG GAGGTGCAAA AGATAGCAAA GCAGCTCATT CAGGCGCTAA ACTACCTGCA CTCTAATCGC ATCATTCACC GCGACATGAA GCCGCAGAAC ATCCTCATAG GCCAGAACGG CGCAGTCAAG CTGGCCGATT TCGGCTTTGC ACGCTCCATG AGCTACAACA CCATCGTATT GACGAGCATC AAGGGCACCC CGCTGTACAT GGCGCCGGAG CTTGTGCAAG AGCGCGCGTA TGACAACCGC GTCGACTTGT GGTCGCTGGG ATGCATTTTA TACGAGCTTT ACTACGGAAA ACCACCGTTC TACACCAACA ACCTGTTTGC CCTGATCAAG AAGATTGTGT GCGAGCCCGT CAAGTTCGAC AACAAGGCGG ACGACCCCAT TAGTCCGGAG TTCAAGAACT TTCTTAGTGG ACTGCTGACC AAGTCCGCCT CATCGCGCCT CAACTGGCCA GACTTGCTGA ATCACCCTTT CGTGCAGCTT ACAAAGGGCG ACGCATCGTG GCAGGATGCC ATCATGCGGC ACGATTCAAA GATGAAGGGT CGAATGGACA AGCTGGGTTG CCTGCGCCTT CACGGAGGTG CCAGCCGGGC CGCGCCAAAG CACCAAGTAG CGCAGAAGGA CGAGATTATC AACGCGCAGA CGGCGTACGT TCTAGAAGCG GGACGCGAGT CCGAGGTGCG GCTGACTCTG GGGCAGCTCG TTGGCGCAGT CAACGCCGCC AGCGGGGACT TGTCGAAGTG CTCTGTCTTG CTTGGCATTT TCGATTTTGG AGTTCTTGAA GCAACGCTCA AGCTCTTTGC CCCAAAGCAG CCGCGCACAA CGGTACAGCT GATCTTGAAC CTGCTGCAGG CGCTGCTGTT TCCGGAGGGC GGTGACGTGG TATCGTTTCC GTCGCAGCGG CCAAAGCGTG ACGGTGTCGA TGTGCTGGTG GAGAAGAACT CTCATCAACA AGTAGACCTG TTTGTTCGTC AGCAAGTTGC AATTGGCCTG ACAAAGAAGC CGCACGACGC TTTAGACTAC ATTATTGGCG AGATCGGCAG CAACAGCAAC GCGCAGGCGG AGGTGTGCGT CAAGATCGTC TTTCAGTGCT TTCGATGGGA GAACAGCTTC GGGCCAACGG TGACGCAGCT GCGCGCCTTT CCTGAGATGT GGTCTGCGGT GCTCTCGTCG GTGGATCACG ACAGCGTTTC CAAAGGCAAG GTGTCGTACG AGTACGCCGC TCTCGTCTTT CACACTGTGT CCATTGTCAT CCCGCAAATC AAGCTGGCAG CCCCGCAGCG CATCAACCGC GAGAAGGTGC TTCACCTTGT AACGCAGGCG CTGTCGGCGG TGTGTTACTA CGAGGTTTCT GCAATTGACG CGCAGCGAAA CAAGATACCG CCGCTCACGT ACGCCGCTGC CGCCGCGCTC CTCATCGCGT TTGCGCACCG CGAGCTCAAG GACATCGTCT CCTTCACCGT CAACGACGCC CTCCTCGACG GGATCTACTC GATTGTCGAC GCCGTGTCCG CAACGCCGAG CCGCCCGATC ACACCGCGCG CGGTGGGCAG CAGTTACGGC TACCCCGACT ACGGCTTGCT CGACGGCGTG GCGCACATGC TGTCCCTCAT CTTTTCGGAC CCGGACTCGC TGGTGTACGC AAAGCTGCCG GGAAAGGCGG AGCACCGCTT CTTGGAGAGC GACACAAAGA ATCTGGCATT GCTGGTCATC GCACTGCTGC GCGACAGCGA CTCGCGGACG GAGTTGTCGC CGAATGGCGT GCAGACACTC TTACGGGCAG CTCAGCAGAT CTTTCAGCAG CAGAAGGAGC AGACCGCGTC GATGAGTCTC TTGATGGACA CTGTCCCGCC TTACGCGGGA GAGTCGGGCA ACCCTTGCTG GCTCGCCGTT GTGTGTCGCA CCCTAAAAAC CGAGTACTTT AAGCAGCTCT TCAACTGGCC CGCTTGCCGT GGCGGTGGTG CCACGGGCGT GAGTGCACAC GTGACGATTG TGTCGCAGAT TCTGGCCGAT TCGCTGCGGC CGGCCAGCTC GTCATCGAAC CCCACCGCGG CGGAGGAGAA GCTGCTGAGT GATGTGAGTC GGGTGCTTTT CAAGGAGCGG GTGGTCGAGC TTCTTGTCAA CGCCATGGAC TTTTCGGAGG GGGTGTTTCT GGGAAGTCCG TTCTCGATCG TGGCGAAGCT GTGCGCCAAC TCGGCTGACT TCATTCGGGC GTTTGTGGAT TGCGGCGGTG TGGAGAGTGC GCGGATTCGG CGCGTGCTGG ACCCTGACAA GGCGGGAACG GGGCTCATGT CCGACGGCCT CGTTGTTCTT TCTCAAATGG CGCGTATGTC TGCCGAATTC TACGAGAGCA TCCACAAGGC AAATCTCTAC GACTGCATCG CCGCGCTGCT GCAGCACTCT GAAAAGGACC TGCGAGGAAA GACGTGCACC CTGATCGGCA ACCTGTGCAA GCACTCGGAC TACTTCTTTC AACCGCTAGA AAGGAACCAC ATCGTGGAAC GCCTCGTGAA GTGCTGCAGC GACAGCGATG CACAGACGCA GAAGCTCGCG GCCTTCGCGA TCGGCAACGC AGCCTTCCAC AGCGACTACC TGTACCGACT GCTGACACCG GCGATTCCGT CGTTGGTGGG GCTGCTGGCC TGCGGGGACG CCAAGACGCG GCAGAACGCA GCCGGCGCGT TGAGTAACTT TGTGCGCAAC GGCGACCAGC TCGTCGGTGC GTTGGAGGCC TCCAAAGTGG TGGAGGCGCT ATTGAAGATG CTGCGTGACG ACGACTCGCT CTCGTCGAAG AAGGTGGCTG TGCTGACAAT CAACTCTTTC TGTACGTACG ACGTCTTCCG TCGCAAGTTT GTAGAGCTGG ATTTGAGAGA GGACATCCGA AAGCTGCAGC AAGATTCCAC GGCGCGCGGG GATCCTTCGA TCCAGAAGTA TGTGGGCAAG CTCATCGACC GCCTTGGATG A
Details for CrfaA.20383.a.B2.GE43782
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHME DYQIIESIGE GSFGKVYKAR IKGTGQIVAM KFIVKKGKNE KELKNLRSEI EILTKLNHPH IIMLFDSFET DSDFVVVMEY AQGELYDILE DDKQLPAKEV QKIAKQLIQA LNYLHSNRII HRDMKPQNIL IGQNGAVKLA DFGFARSMSY NTIVLTSIKG TPLYMAPELV QERAYDNRVD LWSLGCILYE LYYGKPSFYT NNLFALIKKI VCEPVKFDNK ADDPISPEFK NFLSGLLTKS ASSRLNWPDL LNHPFVQLTK GDASWQDAIM RHDSKMKGRM DKLGCLRLHG GASRAAPKHQ VAQKDEIINA QTAYVLEAGR ESEVRLTLGQ LVGAVNAA
Validated NT Sequence
atggctcacc accaccacca ccatatggag gactatcaaa ttatagagtc cattggggag ggctcctttg gcaaggttta caaggcacgc atcaagggaa cgggtcagat tgtagcgatg aaattcattg ttaagaaggg aaagaatgag aaggagctga agaacctgcg cagtgagatc gagattctta ccaaactgaa tcacccccac atcatcatgc tctttgactc atttgagaca gactcggact ttgttgtcgt aatggagtat gcgcagggtg agttgtacga cattctagaa gacgacaaac agctgccggc taaggaggtg caaaagatag caaagcagct cattcaggcg ctaaactacc tgcactctaa tcgcatcatt caccgcgaca tgaagccgca gaacatcctc ataggccaga acggcgcagt caagctggcc gatttcggct ttgcacgctc catgagctac aacaccatcg tattgacgag catcaagggc accccgctgt acatggcgcc ggagcttgtg caagagcgcg cgtatgacaa ccgcgtcgac ttgtggtcgc tgggatgcat tttatacgag ctttactacg gaaaaccatc gttctacacc aacaacctgt ttgccctgat caagaagatt gtgtgcgagc ccgtcaagtt cgacaacaag gcggacgacc ccattagtcc ggagttcaag aactttctta gtggactgct gaccaagtcc gcctcatcgc gcctcaactg gccagacttg ctgaatcacc ctttcgtgca gcttacaaag ggcgacgcat cgtggcagga tgccatcatg cggcacgatt caaagatgaa gggtcgaatg gacaagctgg gttgcctgcg ccttcacgga ggtgccagtc gggccgcgcc aaagcaccaa gtagcgcaga aggacgagat tatcaacgcg cagacggcgt acgttctaga agcgggacgc gagtccgagg tgcggctgac tctggggcag ctcgttggcg cagtcaacgc cgccag
Expected Protein Sequence
MAHHHHHHME DYQIIESIGE GSFGKVYKAR IKGTGQIVAM KFIVKKGKNE KELKNLRSEI EILTKLNHPH IIMLFDSFET DSDFVVVMEY AQGELYDILE DDKQLPAKEV QKIAKQLIQA LNYLHSNRII HRDMKPQNIL IGQNGAVKLA DFGFARSMSY NTIVLTSIKG TPLYMAPELV QERAYDNRVD LWSLGCILYE LYYGKPPFYT NNLFALIKKI VCEPVKFDNK ADDPISPEFK NFLSGLLTKS ASSRLNWPDL LNHPFVQLTK GDASWQDAIM RHDSKMKGRM DKLGCLRLHG GASRAAPKHQ VAQKDEIINA QTAYVLEAGR ESEVRLTLGQ LVGAVNAASG DLSKCS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagg actatcaaat tatagagtcc attggggagg gctcctttgg caaggtttac aaggcacgca tcaagggaac gggtcagatt gtagcgatga aattcattgt taagaaggga aagaatgaga aggagctgaa gaacctgcgc agtgagatcg agattcttac caaactgaat cacccccaca tcatcatgct ctttgactca tttgagacag actcggactt tgttgtcgtg atggagtatg cgcagggtga gttgtacgac attctagaag acgacaaaca gctgccggct aaggaggtgc aaaagatagc aaagcagctc attcaggcgc taaactacct gcactctaat cgcatcattc accgcgacat gaagccgcag aacatcctca taggccagaa cggcgcagtc aagctggccg atttcggctt tgcacgctcc atgagctaca acaccatcgt attgacgagc atcaagggca ccccgctgta catggcgccg gagcttgtgc aagagcgcgc gtatgacaac cgcgtcgact tgtggtcgct gggatgcatt ttatacgagc tttactacgg aaaaccaccg ttctacacca acaacctgtt tgccctgatc aagaagattg tgtgcgagcc cgtcaagttc gacaacaagg cggacgaccc cattagtccg gagttcaaga actttcttag tggactgctg accaagtccg cctcatcgcg cctcaactgg ccagacttgc tgaatcaccc tttcgtgcag cttacaaagg gcgacgcatc gtggcaggat gccatcatgc ggcacgattc aaagatgaag ggtcgaatgg acaagctggg ttgcctgcgc cttcacggag gtgccagccg ggccgcgcca aagcaccaag tagcgcagaa ggacgagatt atcaacgcgc agacggcgta cgttctagaa gcgggacgcg agtccgaggt gcggctgact ctggggcagc tcgttggcgc agtcaacgcc gccagcgggg acttgtcgaa gtgctcttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at