CrfaA.20382.a

serine/threonine-protein kinase, putative

CENTER ID: CrfaA.20382.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.20382.a.HZ2.GE43700 kinase domain 122 567
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:CFAC1_260061400

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKAKKHGKP SKGASKKGNK NDKNKRKAKF VDPTSSVSRD VRDSVAALVE EIERSEPVEE ASPQPIDKAE KKRQQLAEKE GISVKSGMTK KQALKIANRL KAQQSTTHQS DGDRDEESGG DDGNGTEEDY SDTANEPARE YRKGGYHHVV VGEIYNDRYR IVKKLGWGYF STVWLVWDYE KERYQAMKIQ KSAASYSEAA YDEIKLLTEI MEADPHKNHC CARLNDYFKH SGPNGTHVCM VFDVYGENLL SLMERYEYRG IPLPIVKCIA RQVLIGLDHI NSIDIIHTDL KPENVLLSTP KHSIISLMKR FHPPPLHQRP KLTERDPKTM TKSQRRRYYK KLAKEERRVL LGEDDDGNDS DNDNESNGNA EVDSEGSKTD PEWEVERFHH VILADFGNSC WTYKQFTDEV QTRQYRCPEV ILGEPYSTPI DVWSCACMLF ELITGQFLFD PKKGDDYSRD EDHLALMTEL LGDLPENMRL GSGKFRSHYY TSKGELRNIK DLQYWNLEDV LHQRHKFTRK KAKEIADFLL PMLEYNPDSR ATPSAMLRDH DAFFDIEDDD YAPLCFVDSG SGDDSLASDE EDDAGSEDSG DSFSSASSSA RKLSRSRQCD NDADRTEAFR YWEEHPILNR SYLEERGLTI ADIQSVLAGN LLESREAQEA AAEIIRLLSD DAEFLSTDRG SDGHEADKED EGDEDYDDDD DDDDDPSLQH DGTTAPRTAG GGAEHAKDEE DEESDEESDE ESEDDAP
NT Sequence
ATGGGGAAAG CGAAGAAGCA TGGAAAGCCC TCGAAAGGGG CCTCCAAGAA GGGCAACAAG AATGACAAGA ACAAGCGCAA GGCAAAGTTT GTAGATCCGA CCAGCAGCGT CAGCCGCGAC GTCCGCGACA GCGTGGCGGC GCTGGTGGAG GAGATCGAGC GCAGCGAGCC GGTAGAGGAG GCGAGTCCGC AGCCCATCGA CAAGGCGGAG AAGAAGCGCC AGCAGCTCGC CGAGAAGGAG GGTATTTCCG TCAAGAGTGG CATGACCAAA AAGCAAGCGC TCAAGATCGC CAACCGCCTC AAGGCGCAGC AGAGCACCAC CCACCAAAGC GACGGCGACC GCGACGAGGA GAGCGGCGGC GACGACGGCA ACGGGACGGA GGAGGACTAC AGCGACACGG CGAACGAGCC GGCGCGGGAG TACCGCAAGG GCGGCTACCA CCACGTCGTC GTCGGCGAGA TCTACAACGA CCGCTACCGC ATTGTTAAGA AGCTCGGCTG GGGCTACTTC TCCACCGTGT GGCTTGTCTG GGATTACGAG AAGGAGCGCT ACCAGGCCAT GAAGATCCAG AAGTCTGCCG CCAGCTACAG CGAGGCAGCC TACGACGAGA TCAAGCTGCT GACGGAGATC ATGGAGGCGG ACCCGCACAA GAACCACTGC TGTGCACGCC TGAACGACTA CTTCAAGCAC TCGGGCCCTA ACGGCACGCA CGTGTGCATG GTGTTCGATG TCTACGGCGA GAATTTGCTC TCCCTCATGG AGCGCTACGA GTACCGCGGC ATCCCGCTGC CCATCGTCAA GTGCATTGCC CGTCAGGTGC TCATCGGGCT CGACCACATC AACTCCATCG ACATCATTCA CACCGACTTG AAGCCGGAGA ACGTGCTGCT CTCGACGCCG AAGCACTCCA TCATCTCTCT CATGAAGCGC TTCCACCCGC CCCCGCTGCA CCAGCGGCCG AAGCTGACGG AGCGCGACCC CAAGACGATG ACCAAGTCGC AGCGGCGGCG CTACTACAAG AAGCTTGCGA AGGAAGAGCG GCGCGTGTTG CTTGGCGAGG ACGACGACGG CAACGACAGC GACAACGACA ACGAGAGCAA CGGCAACGCC GAGGTGGACT CGGAGGGCAG CAAGACGGAC CCGGAGTGGG AGGTGGAGCG CTTCCACCAC GTCATCCTCG CCGACTTTGG CAACAGCTGC TGGACCTACA AGCAGTTCAC GGACGAGGTG CAGACGCGGC AGTACCGCTG CCCCGAGGTG ATCCTCGGCG AGCCCTACTC CACCCCCATC GACGTCTGGT CCTGCGCCTG CATGCTCTTC GAGCTCATCA CCGGCCAGTT CCTCTTCGAC CCCAAGAAGG GCGACGACTA CTCCCGCGAC GAGGACCACC TCGCCCTCAT GACGGAGCTC CTCGGCGACT TGCCAGAGAA CATGCGACTC GGCTCCGGCA AGTTCCGCAG CCACTACTAC ACCTCAAAGG GGGAGCTGCG CAACATCAAG GACCTCCAGT ACTGGAACCT AGAGGACGTG CTGCACCAGC GACACAAGTT CACGCGCAAG AAGGCGAAGG AGATTGCGGA CTTTCTGCTG CCGATGCTCG AGTACAACCC CGACTCCCGC GCCACGCCCT CGGCGATGCT GCGCGACCAC GACGCGTTTT TCGACATCGA GGACGACGAT TACGCCCCGC TGTGCTTTGT CGATAGCGGC AGCGGCGATG ACTCGCTGGC GTCCGACGAG GAGGACGACG CGGGCAGCGA GGACTCGGGC GACTCCTTCT CCTCCGCCTC TTCGAGTGCC CGGAAGCTGA GCCGGTCACG CCAGTGTGAC AACGACGCCG ACCGCACCGA GGCCTTCCGC TACTGGGAGG AGCACCCGAT CCTCAACCGC TCCTACTTGG AGGAGCGCGG GCTGACAATC GCCGACATCC AAAGCGTGCT GGCCGGCAAC CTGCTGGAGA GCCGCGAGGC GCAGGAGGCG GCTGCGGAGA TTATCCGCCT TCTCTCCGAC GATGCCGAGT TCTTGTCAAC CGACCGCGGC AGTGACGGCC ACGAAGCGGA CAAGGAGGAC GAGGGCGATG AGGACTACGA CGACGACGAT GACGATGATG ATGATCCGTC CTTACAGCAC GACGGCACCA CTGCTCCCCG CACTGCTGGC GGTGGTGCTG AGCACGCCAA GGACGAGGAG GACGAGGAGA GCGATGAGGA GAGCGACGAG GAGAGCGAGG ACGATGCGCC GTAG
Details for CrfaA.20382.a.HZ2.GE43700
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSDG NGTEEDYSDT ANEPAREYRK GGYHHVVVGE IYNDRYRIVK KLGWGYFSTV WLVWDYEKER YQAMKIQKSA ASYSEAAYDE IKLLTEIMEA DPHKNHCCAR LNDYFKHSGP NGTHVCMVFD VYGENLLSLM ERYEYRGIPL PIVKCIARQV LIGLDHINSI DIIHTDLKPE NVLLSTPKHS IISLMKRFHP PPLHQRPKLT ERDPKTMTKS QRRRYYKKLA KEERRVLLGE DDDGNDSDND NESNGNAEVD SEGSKTDPEW EVERFHHVIL ADFGNSCWTY KQFTDEVQTR QYRCPEVILG EPYSTPIDVW SCACMLFELI TGQFLFDPKK GDDYSRDEDH LALMTELLGD LPENMRLGSG KFRSHYYTSK GELRNIKDLQ YWNLEDVLHQ RHKFTRKKAK EIADFLLPML EYNPDSRATP SAMLRDHDAF FDIEDDDYAP LCFV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg acggcaacgg gacggaggag gactacagcg acacggcgaa cgagccggcg cgggagtacc gcaagggcgg ctaccaccac gtcgtcgtcg gcgagatcta caacgaccgc taccgcattg ttaagaagct cggctggggc tacttctcca ccgtgtggct tgtctgggat tacgagaagg agcgctacca ggccatgaag atccagaagt ctgccgccag ctacagcgag gcagcctacg acgagatcaa gctgctgacg gagatcatgg aggcggaccc gcacaagaac cactgctgtg cacgcctgaa cgactacttc aagcactcgg gccctaacgg cacgcacgtg tgcatggtgt tcgatgtcta cggcgagaat ttgctctccc tcatggagcg ctacgagtac cgcggcatcc cgctgcccat cgtcaagtgc attgcccgtc aggtgctcat cgggctcgac cacatcaact ccatcgacat cattcacacc gacttgaagc cggagaacgt gctgctctcg acgccgaagc actccatcat ctctctcatg aagcgcttcc acccgccccc gctgcaccag cggccgaagc tgacggagcg cgaccccaag acgatgacca agtcgcagcg gcggcgctac tacaagaagc ttgcgaagga agagcggcgc gtgttgcttg gcgaggacga cgacggcaac gacagcgaca acgacaacga gagcaacggc aacgccgagg tggactcgga gggcagcaag acggacccgg agtgggaggt ggagcgcttc caccacgtca tcctcgccga ctttggcaac agctgctgga cctacaagca gttcacggac gaggtgcaga cgcggcagta ccgctgcccc gaggtgatcc tcggcgagcc ctactccacc cccatcgacg tctggtcctg cgcctgcatg ctcttcgagc tcatcaccgg ccagttcctc ttcgacccca agaagggcga cgactactcc cgcgacgagg accacctcgc cctcatgacg gagctcctcg gcgacttgcc agagaacatg cgactcggct ccggcaagtt ccgcagccac tactacacct caaaggggga gctgcgcaac atcaaggacc tccagtactg gaacctagag gacgtgctgc accagcgaca caagttcacg cgcaagaagg cgaaggagat tgcggacttt ctgctgccga tgctcgagta caaccccgac tcccgcgcca cgccctcggc gatgctgcgc gaccacgacg cgtttttcga catcgaggac gacgattacg ccccgctgtg ctttgtctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa