CrfaA.19530.a

J-binding protein 1 (JBP1)

CENTER ID: CrfaA.19530.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrfaA.19530.a.B2.GE41388 C-term domain 569 809
CrfaA.19530.a.B3.GE41389 Tet-JBP oxygenase 67 285
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79416
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:CFAC1_050026200
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPKSKKVKQ DIFNFPDGKD VPTTKEKAEA YVDALKAHPF YDNVHSVVDV YDSATLRDGK GRVIGVMLRK ALPEHATTAA SGLLSAAAVR TSLRSSMFGG ESPLSGIAGY FDYRGSPVEL KARKTAFTYE HEKKWPAVFP LVDYVSEIYK SVMPEHWAAQ DSAIPDIVRI HGTPFSTLTI NSRFRTASHT DAGDFDGGYS CIACIDGDFK GLALGFDDFH VNVPMQPRDV LVFDSHYFHS NSELEISCPT EDWRRLTCVF YYRSALGEPS SYAEYRRRLA AAQQDSTAQP VVSSVVEKPN GKNLYKPSTV FPIDPTPFAV VAQLHRLHHC AAKGLCVHEL LAVPSSPLAV LLFGERLSCS DGIPLRAAEQ KLKANADGAS RGVTSSGGFS ESDAVLTTAV EKSKYLERDH LSQCISAELL AMWVEARKHW LRLVATEWAR MIATAPERTD FLWKNKSPMN TAFFDLCEVA KQVMLGLLDK ETATPTEERH FWSVYAAHLH RACAERLMMP EEAMSLRKLN VKLKDFSFGG TRYFKDMPVE EQERRVARKA SIEEARRRST AAKDGEQRSN WLTNDAFDYQ TEDCEVDYAG HGWAVPTQHA KTVTANVHQE AVAATTEAVR VLIVLPRPPS GDRGDAAVDL PKEATASAEW VRLMSSPAVR RVLAAKQRNL TLLPNCNVEA VSLNFAYHDS LPQKATFDFV VLQHVLSAMP EDAIATEYVS RMRSICTGCL FVVETDVQCR QYFTLHYPLR VQYDAVAPAF FQLLHRRSYG TPLARTRTKA EVEALFPFVC CARYKLQGSP MNTVVHLLAL E
NT Sequence
atggagccaa agtcaaagaa ggtgaagcag gacattttca acttccctga cggcaaagat gtccccacaa caaaggagaa ggcggaggcg tacgtggacg cgttaaaggc gcaccccttc tacgacaacg tgcactccgt cgttgatgtg tatgacagcg ccacgctgcg cgacggcaag gggcgtgtca tcggcgttat gctgcgcaag gccttgcccg agcatgccac cacggcggcc tccgggttgc tcagcgctgc agcggtgcgc acgtcactgc ggagctccat gtttggcggg gagtctccgc tgagcggcat cgccggctac ttcgactacc gtggctctcc agtcgagcta aaagcgcgca agacggcctt tacgtacgag catgagaaaa agtggcctgc cgtcttcccc ctcgtcgact acgtgagtga gatctacaag tctgtgatgc cggagcactg ggcggcgcag gacagcgcca tccctgacat cgtccgcatc cacggcacac ccttctctac gctcacaatc aactcacgct tccgcacggc gtcgcacacg gacgcgggtg acttcgatgg cggctacagc tgcatcgcgt gcatcgatgg cgacttcaaa ggccttgccc tcggtttcga cgacttccac gtgaacgtgc caatgcagcc gcgcgatgtc ctcgtcttcg actcgcacta cttccactcc aacagcgagc tggagatcag ctgccccacc gaggattggc gccgcctcac ctgcgtcttc tactatcgct ccgcgctggg tgagccgagc agctacgcgg agtaccgccg tcgactcgcc gcggcacagc aggacagcac cgcccagccg gtcgtatcct ccgtggtgga gaagcccaac gggaaaaacc tgtacaagcc ctccaccgtc ttcccgattg acccgactcc tttcgcggtc gtcgcacagc tgcaccgtct gcaccactgc gccgcaaagg gcctttgtgt gcacgagctt ctcgctgtgc cgtcctcccc cctcgcggtg ctgttgttcg gggagcggct gagctgctca gacggcatcc ctctccgcgc ggccgagcag aaattgaagg caaacgccga cggcgcgtcg cgcggtgtca ctagcagtgg cggcttcagc gagagcgacg cggtgctgac aacggcggtg gagaagagca agtacctcga gcgagaccac ctctcgcagt gcatcagcgc ggagctgctg gcgatgtggg tggaggcgcg gaagcattgg ctgcggctcg tcgcgacgga gtgggcacgt atgatcgcca ccgcgccgga gcgcaccgac tttctgtgga aaaacaagtc ccccatgaac accgcctttt tcgacctctg tgaggtggca aagcaggtga tgttggggct gctggacaag gagacagcga cgccaacaga ggagcggcac ttctggtccg tgtacgccgc ccacctccac cgcgcctgcg ctgagaggct gatgatgccc gaggaggcga tgtctctccg caagctcaac gtgaagctga aggacttcag ctttggcggc acccgctact tcaaggacat gcctgtcgag gagcaggagc ggcgggtggc tcgcaaggca agcatcgagg aagcacgccg ccgcagcacc gcggccaagg acggcgagca gcggtccaac tggctcacca acgacgcctt cgactaccaa acggaggact gcgaggtgga ctacgcgggg cacggctggg cggtgccgac gcagcacgca aagacggtga cggccaacgt gcatcaggag gcggtggcgg cgacgacaga ggcggtgcgt gtgctgattg tgctgccgcg gccaccgagc ggggatcgcg gtgacgccgc cgttgacctg ccgaaggaag cgacggcgtc ggcggagtgg gtgcgtctga tgagcagccc ggcggtgcgc cgcgttctgg ccgccaagca gcgcaactta acgctgctgc ccaactgcaa cgtcgaggcg gtctcgctga actttgccta ccacgacagc ctgccgcaga aggcgacgtt tgattttgtg gtgctgcagc acgtgctgag tgcgatgcca gaggacgcga tcgcgacgga atacgtgtcc cgcatgcgct ccatctgcac aggctgcctc tttgttgtcg aaacggatgt gcagtgccgt cagtacttca ccctccacta cccgctccgt gtgcagtacg acgccgtggc gcctgccttt ttccagcttc tgcaccgccg cagctacggc accccgctgg ctcgcacccg caccaaagca gaggtagagg ctctctttcc ctttgtgtgc tgcgcacggt acaagctgca gggctccccc atgaataccg tcgtgcacct tcttgctttg gagtga
Details for CrfaA.19530.a.B2.GE41388
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS NWLTNDAFDY QTEDCEVDYA GHGWAVPTQH AKTVTANVHQ EAVAATTEAV RVLIVLPRPP SGDRGDAAVD LPKEATASAE WVRLMSSPAV RRVLAAKQRN LTLLPNCNVE AVSLNFAYHD SLPQKATFDF VVLQHVLSAM PEDAIATDYV SRMRSICTGC LFVVETDVQC RQYFTLHYPL RVQYDAVAPA FFQLLHRRSY GTPLARTRTK AEVEALFPFV CCARYKLQGS PMNTVVHLLA
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc aactggctca ccaacgacgc cttcgactac caaacggagg actgcgaggt ggactacgcg gggcacggct gggcggtgcc gacgcagcac gcaaagacgg tgacggccaa cgtacatcag gaggcggtgg cggcgacgac agaggcggtg cgtgtgctga ttgtgctgcc gcggccaccg agcggggatc gcggtgacgc cgccgttgac ctgccgaagg aagcgacggc gtcggcggag tgggtgcgtc tgatgagcag cccggcggtg cgccgcgttc tggccgccaa gcagcgcaac ttaacgctgc tgcccaactg caacgtcgag gcggtctcgc tgaactttgc ctatcacgac agcctgccgc agaaggcgac gtttgatttt gtggtgctgc agcacgtgct gagtgcgatg ccagaggacg cgatcgcgac ggactacgtg tcccgcatgc gctccatctg cacaggctgc ctctttgttg tcgaaacgga tgtgcagtgc cgtcagtact tcaccctcca ctacccgctc cgtgtgcagt acgacgccgt ggcgcctgcc tttttccagc ttctgcaccg ccgcagctac ggcaccccgc tggctcgcac ccgcaccaaa gcagaggtag aggctctctt tccctttgtg tgctgcgcac ggtacaagct gcagggctcc cccatgaata ccgtcgtgca ccttcttgct taagtgagta agat
Expected Protein Sequence
MAHHHHHHSN WLTNDAFDYQ TEDCEVDYAG HGWAVPTQHA KTVTANVHQE AVAATTEAVR VLIVLPRPPS GDRGDAAVDL PKEATASAEW VRLMSSPAVR RVLAAKQRNL TLLPNCNVEA VSLNFAYHDS LPQKATFDFV VLQHVLSAMP EDAIATEYVS RMRSICTGCL FVVETDVQCR QYFTLHYPLR VQYDAVAPAF FQLLHRRSYG TPLARTRTKA EVEALFPFVC CARYKLQGSP MNTVVHLLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccaact ggctcaccaa cgacgccttc gactaccaaa cggaggactg cgaggtggac tacgcggggc acggctgggc ggtgccgacg cagcacgcaa agacggtgac ggccaacgtg catcaggagg cggtggcggc gacgacagag gcggtgcgtg tgctgattgt gctgccgcgg ccaccgagcg gggatcgcgg tgacgccgcc gttgacctgc cgaaggaagc gacggcgtcg gcggagtggg tgcgtctgat gagcagcccg gcggtgcgcc gcgttctggc cgccaagcag cgcaacttaa cgctgctgcc caactgcaac gtcgaggcgg tctcgctgaa ctttgcctac cacgacagcc tgccgcagaa ggcgacgttt gattttgtgg tgctgcagca cgtgctgagt gcgatgccag aggacgcgat cgcgacggaa tacgtgtccc gcatgcgctc catctgcaca ggctgcctct ttgttgtcga aacggatgtg cagtgccgtc agtacttcac cctccactac ccgctccgtg tgcagtacga cgccgtggcg cctgcctttt tccagcttct gcaccgccgc agctacggca ccccgctggc tcgcacccgc accaaagcag aggtagaggc tctctttccc tttgtgtgct gcgcacggta caagctgcag ggctccccca tgaataccgt cgtgcacctt cttgcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for CrfaA.19530.a.B3.GE41389
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML RKALPEHATT AASGLLSAAA VRTSLRSSMF GGESPLSGIA GYFDYRGSPV ELKARKTAFT YEHEKKWPAV FPLVDYVSEI YKSVMPEHWA AQDSAIPDIV RIHGTPFSTL TINSRFRTAS HTDAGDFDGG YSCIACIDGD FKGLALGFDD FHVNVPMQPR DVLVFDSHYF HSNSELEISC PTEDWRRLTC VFYYRSALGE PSSYAEYRRR LAAAQQD
Validated NT Sequence
atggctcacc accaccacca ccatatgctg cgcaaggcct tgcccgagca tgccaccacg gcggcctccg ggttgctcag cgctgcagcg gtgcgcacgt cactgcggag ttccatgttt ggcggggagt ctccgctgag cggcatcgcc ggctacttcg actaccgtgg ctctccagtc gagcttaaag cgcgcaagac agcctttact tacgagcatg agaaaaagtg gcctgccgtc ttccccctcg tcgactacgt gagtgagatc tacaagtctg tgatgccgga gcactgggcg gcgcaggaca gcgccatccc tgacatcgtc cgcatccacg gcacaccctt ctctacgctc accatcaact cacgcttccg cacggcgtcg cacacggacg cgggtgactt cgatggcggc tacagctgca tcgcgtgcat cgatggcgac ttcaaaggcc ttgccctcgg tttcgacgac ttccacgtga acgtgccaat gcagccgcgc gatgtcctcg tcttcgactc gcactacttc cactccaaca gcgagctgga gatcagctgc cccaccgagg attggcgccg cctcacctgc gtcttctact atcgctccgc gctgggtgag ccgagcagct acgcggagta ccgccgtcga ctcgccgcgg cacagcagga ctaagtgagt aagat
Expected Protein Sequence
MAHHHHHHML RKALPEHATT AASGLLSAAA VRTSLRSSMF GGESPLSGIA GYFDYRGSPV ELKARKTAFT YEHEKKWPAV FPLVDYVSEI YKSVMPEHWA AQDSAIPDIV RIHGTPFSTL TINSRFRTAS HTDAGDFDGG YSCIACIDGD FKGLALGFDD FHVNVPMQPR DVLVFDSHYF HSNSELEISC PTEDWRRLTC VFYYRSALGE PSSYAEYRRR LAAAQQD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcaaggcctt gcccgagcat gccaccacgg cggcctccgg gttgctcagc gctgcagcgg tgcgcacgtc actgcggagc tccatgtttg gcggggagtc tccgctgagc ggcatcgccg gctacttcga ctaccgtggc tctccagtcg agctaaaagc gcgcaagacg gcctttacgt acgagcatga gaaaaagtgg cctgccgtct tccccctcgt cgactacgtg agtgagatct acaagtctgt gatgccggag cactgggcgg cgcaggacag cgccatccct gacatcgtcc gcatccacgg cacacccttc tctacgctca caatcaactc acgcttccgc acggcgtcgc acacggacgc gggtgacttc gatggcggct acagctgcat cgcgtgcatc gatggcgact tcaaaggcct tgccctcggt ttcgacgact tccacgtgaa cgtgccaatg cagccgcgcg atgtcctcgt cttcgactcg cactacttcc actccaacag cgagctggag atcagctgcc ccaccgagga ttggcgccgc ctcacctgcg tcttctacta tcgctccgcg ctgggtgagc cgagcagcta cgcggagtac cgccgtcgac tcgccgcggc acagcaggac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat