CrfaA.19529.a

J2IP1

CENTER ID: CrfaA.19529.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.19529.a.B1.GE41386 full length 1 245
CrfaA.19529.a.B2.GE41387 ordered domain 13 239
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:CFAC1_060005900

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTASDKAH LVPLLEFLQF VSKKGKEGKA RFPRKINGAD DRIADGTDII FEQDWPPVSP GLDCVFRRSE SLDDSTQLSQ DSSVGFSSAM DRRKRQRSPD SSTEATFNSV PSRDDFGDAE KSRETGSFAT RSFAFSLGDL GLELLVDINR QAVDREVAAR IQGMRCCYLF LGTSMIVTGD VADVNLSGKT VLVSPDSELR VEMEWVSLSR VYVEPEDVEL VREEAARRIE TIMEAEMEGK RPPKA
NT Sequence
atgtcttcga ctgccagcga taaagcacac cttgtgccgc tgctcgagtt cctccaattt gtttcgaaaa agggaaaaga aggcaaggct cgcttccctc gcaaaatcaa tggcgcggac gaccgaattg ccgacgggac agatataatt tttgagcaag attggcctcc agtgtcgccg ggcttggact gcgtgttccg acgcagcgaa agtctggatg actccactca gctttcccaa gacagtagcg tcggattcag ttctgctatg gaccgtagaa agcgtcagcg ctcgcctgac agcagcacgg aggcgacttt caactcagtg ccttctcgcg acgatttcgg agatgcagag aagtcacgcg agacaggcag ctttgcgaca aggtcttttg cgttttcgct gggcgatctc ggtctagagc tacttgtcga catcaatcgc caggctgtag acagggaagt cgctgctcgg attcaaggaa tgcggtgctg ctatctgttt ctggggactt ccatgattgt gacgggtgac gttgccgacg tcaatctttc gggcaagacg gtcttggtat ccccggatag cgagctgcgt gtagagatgg agtgggtttc tttaagccga gtgtacgttg agccggagga tgtggagcta gtacgcgaag aggcagcgcg gaggattgag accatcatgg aggctgagat ggaaggcaag cgaccaccga aagcatag
Details for CrfaA.19529.a.B1.GE41386
HARVESTED ON: data unavailable
SEQUENCED ON: 4/27/2017
EXPECTED MW: 28kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS STASDKAHLV PLLEFLQFVS KKGKEGKARF PRKINGADDR IADGTDIIFE QDWPPVSPGL DCVFRRSESL DDSTQLSQDS SVGFSSAMDR RKRQRSPDSS TEATFNSVPS RDDFGDAEKS RETGSFATRS FAFSLGDLGL ELLVDINRQA VDREVAARIQ GMRCCYLFLG TSMIVTGDVA DVNLSGKTVL VSPDSELRVE MEWVSLSRVY VEPEDVELVR EEAARRIETI MEAEMEGKRP PKA
Validated NT Sequence
atggctcacc accaccacca ccatatgtct tcgactgcca gcgataaagc acaccttgtg ccgctgctcg agttcctcca atttgtttcg aaaaagggaa aagaaggcaa ggctcgcttc cctcgcaaaa tcaatggcgc ggacgaccga attgccgacg ggacagatat aatttttgag caagattggc ctccagtgtc gccgggcttg gactgcgtgt tccgacgcag cgaaagtctg gatgactcca ctcagctttc ccaagacagt agcgtcggat tcagttctgc tatggaccgt agaaagcgtc agcgctcgcc tgacagcagc acggaggcga ctttcaactc agtgccttct cgcgacgatt tcggagatgc agagaagtca cgcgagacag gcagctttgc gacaaggtct tttgcgtttt cgctgggcga tctcggtcta gagctacttg tcgacatcaa tcgccaggct gtagacaggg aagtcgctgc tcggattcaa ggaatgcggt gctgctatct gtttctgggg acttccatga ttgtgacggg tgacgttgcc gacgtcaatc tttcgggcaa gacggtcttg gtatccccgg atagcgagct gcgtgtagag atggagtggg tttctttaag ccgagtgtac gttgagccgg aggatgtgga gctagtacgc gaagaggcag cgcggaggat tgagaccatc atggaggctg agatggaagg caagcgacca ccgaaagcat aagtgagtaa gat
Expected Protein Sequence
MAHHHHHHMS STASDKAHLV PLLEFLQFVS KKGKEGKARF PRKINGADDR IADGTDIIFE QDWPPVSPGL DCVFRRSESL DDSTQLSQDS SVGFSSAMDR RKRQRSPDSS TEATFNSVPS RDDFGDAEKS RETGSFATRS FAFSLGDLGL ELLVDINRQA VDREVAARIQ GMRCCYLFLG TSMIVTGDVA DVNLSGKTVL VSPDSELRVE MEWVSLSRVY VEPEDVELVR EEAARRIETI MEAEMEGKRP PKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt cgactgccag cgataaagca caccttgtgc cgctgctcga gttcctccaa tttgtttcga aaaagggaaa agaaggcaag gctcgcttcc ctcgcaaaat caatggcgcg gacgaccgaa ttgccgacgg gacagatata atttttgagc aagattggcc tccagtgtcg ccgggcttgg actgcgtgtt ccgacgcagc gaaagtctgg atgactccac tcagctttcc caagacagta gcgtcggatt cagttctgct atggaccgta gaaagcgtca gcgctcgcct gacagcagca cggaggcgac tttcaactca gtgccttctc gcgacgattt cggagatgca gagaagtcac gcgagacagg cagctttgcg acaaggtctt ttgcgttttc gctgggcgat ctcggtctag agctacttgt cgacatcaat cgccaggctg tagacaggga agtcgctgct cggattcaag gaatgcggtg ctgctatctg tttctgggga cttccatgat tgtgacgggt gacgttgccg acgtcaatct ttcgggcaag acggtcttgg tatccccgga tagcgagctg cgtgtagaga tggagtgggt ttctttaagc cgagtgtacg ttgagccgga ggatgtggag ctagtacgcg aagaggcagc gcggaggatt gagaccatca tggaggctga gatggaaggc aagcgaccac cgaaagcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for CrfaA.19529.a.B2.GE41387
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP LLEFLQFVSK KGKEGKARFP RKINGADDRI ADGTDIIFEQ DWPPVSPGLD CVFRRSESLD DSTQLSQDSS VGFSSAMDRR KRQRSPDSST EATFNSVPSR DDFGDAEKSR ETGSFATRSF AFSLGDLGLE LLVDINRQAV DREVAARIQG MRCCYLFLGT SMIVTGDVAD VNLSGKTVLV SPDSELRVEM EWVSLSRVYV EPEDVELVRE EAARRIETIM EAEMEG
Validated NT Sequence
atggctcacc accaccacca ccatatgccg ctgctcgagt tcctccaatt tgtttcgaaa aagggaaaag aaggcaaggc tcgcttccct cgcaaaatca atggcgcgga cgaccgaatt gccgacggga cagatataat ttttgagcaa gattggcctc cagtgtcgcc gggcttggac tgcgtgttcc gacgcagcga aagtctggat gactccactc agctttccca agacagtagc gtcggattca gttctgctat ggaccgtaga aagcgtcagc gctcgcctga cagcagcacg gaggcgactt tcaactcagt gccttctcgc gacgatttcg gagatgcaga gaagtcacgc gagacaggca gctttgcgac aaggtctttt gcgttttcgc tgggcgatct cggtctagag ctacttgtcg acatcaatcg ccaggctgta gacagggaag tcgctgctcg gattcaagga atgcggtgct gctatctgtt tctggggact tccatgattg tgacgggtga cgttgccgac gtcaatcttt cgggcaagac ggtcttggta tccccggata gcgagctgcg tgtagagatg gagtgggttt ctttaagccg agtgtacgtt gagccggagg atgtggagct agtacgcgaa gaggcagcgc ggaggattga gaccatcatg gaggctgaga tggaaggcta agtgagtaag at
Expected Protein Sequence
MAHHHHHHPL LEFLQFVSKK GKEGKARFPR KINGADDRIA DGTDIIFEQD WPPVSPGLDC VFRRSESLDD STQLSQDSSV GFSSAMDRRK RQRSPDSSTE ATFNSVPSRD DFGDAEKSRE TGSFATRSFA FSLGDLGLEL LVDINRQAVD REVAARIQGM RCCYLFLGTS MIVTGDVADV NLSGKTVLVS PDSELRVEME WVSLSRVYVE PEDVELVREE AARRIETIME AEMEG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccgctgc tcgagttcct ccaatttgtt tcgaaaaagg gaaaagaagg caaggctcgc ttccctcgca aaatcaatgg cgcggacgac cgaattgccg acgggacaga tataattttt gagcaagatt ggcctccagt gtcgccgggc ttggactgcg tgttccgacg cagcgaaagt ctggatgact ccactcagct ttcccaagac agtagcgtcg gattcagttc tgctatggac cgtagaaagc gtcagcgctc gcctgacagc agcacggagg cgactttcaa ctcagtgcct tctcgcgacg atttcggaga tgcagagaag tcacgcgaga caggcagctt tgcgacaagg tcttttgcgt tttcgctggg cgatctcggt ctagagctac ttgtcgacat caatcgccag gctgtagaca gggaagtcgc tgctcggatt caaggaatgc ggtgctgcta tctgtttctg gggacttcca tgattgtgac gggtgacgtt gccgacgtca atctttcggg caagacggtc ttggtatccc cggatagcga gctgcgtgta gagatggagt gggtttcttt aagccgagtg tacgttgagc cggaggatgt ggagctagta cgcgaagagg cagcgcggag gattgagacc atcatggagg ctgagatgga aggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat