CrfaA.19528.a

J-binding protein 2 (JBP2)

CENTER ID: CrfaA.19528.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.19528.a.B3.GE41384 Tet_JBP oxygenase 240 513
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79432
de-novo with evolutionary constraints ModelArchive_ma-9en24
de-novo with evolutionary constraints ModelArchive_ma-qvh2h
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:CFAC1_110005600
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGGLPRAGP SPELIAVADL VQTTGEIAVV FPSLSIGDLE AITDETQRRQ LRIAGIPRGG YTLLPAIPLE DDELLRLCEE YCACGEADKA VIRGSLYTRH YPLFDRRRGD ARPLYHPAEY INRIIQFCNY YAQVPSSELP ALGEQCPFLH ISPVKEICFY LRCNSLDATD FSLQEDEKTS LSLLRLHARE DVERVMGGAS PTSPSPLPDG SALLPGEAVG TAAEPELLFQ TGAVEDAGDD ARERRDDLTG EETVDAVHGA VSEYVSIEGL NLVTKTSIFY DREGDGQRVV AVYIRGGLPT DVCEAAATVL EPAATKKNLR AATNGGVPPD TGIVGYYDYF TNPTPHKCRE TEFSRRNWSS FSRCEPFLRQ LDKLYSRMAP MHHHLQKVAI PTQYQLCDTV FSTITVNRNF RTAVHTDKGD FRSGLGVLAV INGKFEGCHL AIKKINKAFE LKTGDVLLFD TSLEHGNTEV TSAADDWYRT SVVCYLRTGL MSSVCEMERR KHLNRLIVQQ LQSAAVREAT VNINGADSSL PPLFVPTRLA SQLAPVQLSA LGFIVERAEK QSGCVVAMTM GLGKTLVALT VCFSRLHLAP QSDILILTPK PIISHWVDEK NKWGAYGLHF SHFVASDGLN NLEFEQQLLE YDRQRTNKRP RTGHVFVINS ELLSGFLRRF RHFTPSLIIV DEGHRVASKG NKMTESLERL RCNLRVVLSG TPLQNDASEL YRVVGWVNRG VAKVLPPRRF QELANEVNHF IQGDDGAFQS AVVALEYIQD WMRGFVFREM ENDLPPLHDY LLVCGSSDVQ KEYEAKIGLT DGATASLKAT EHRPHHLSTH PACYLAFVSD SYQSMLVGPA ARERESAANS RVTSLDEVDT TRLERYAQMV ETDHLDSFID VSGKMRVLVD IVRRVQAKRE KLIVFSLYVG SQDLIHRTLT ALRVCTFTVR GRDSQDRRRR AMHEFSNNEN LMVLVASTKV AAYGLDFTAA NHVVLFDSWW NPQVDAQAIA RAYRRNQKKP VTVYRLISAT ENKSVLRLQT RKIALFRCIL HERPSRAATV DEIEDCAATE KDEERRAFWL RLKSSPLSGG FPALVNVYRY QESVRESV
NT Sequence
atgtcgggtg ggcttccgcg ggctggacct tctccagagc ttattgcagt tgccgatctc gtgcagacaa ccggcgaaat cgccgtcgtc ttcccctcgc tctccattgg ggatctcgag gccatcacag acgagacgca gcggcggcag ctgcgcattg cgggaatccc acgaggcggc tacacgcttc tccctgcgat cccgctcgag gacgatgagc tgctgcgtct gtgcgaggag tactgcgcct gcggcgaggc ggacaaggcg gtcatccgcg gcagtctcta cacccggcac tacccactct tcgaccggcg aagaggcgac gcacggccgc tctaccaccc cgccgagtac atcaaccgga tcattcagtt ctgcaactac tacgcgcagg tgccgagcag tgagctgccg gcgttgggag agcagtgccc ttttcttcac atctccccgg tgaaggagat atgcttctac ctccgctgca actccctcga cgcgacagat ttttcgctgc aggaggacga gaagacttct ctgtcgctgc tgcggctgca cgccagggag gatgtggaga gggtgatggg tggcgcgtcg cccacatcgc cctcgcctct gccagacggc tctgctctgt taccgggtga ggcagtcggg actgccgcgg agccggagct gctgttccag acaggcgccg tcgaggacgc cggcgacgac gcacgcgagc ggagggacga cctgacgggc gaggaaaccg tcgacgccgt ccacggcgcc gtctcggagt acgtctcgat cgagggcctc aacctcgtga ccaagacatc catcttctac gaccgcgagg gcgacggcca gcgcgtcgtg gcggtgtaca tccgcggcgg cctcccgaca gacgtttgcg aggcggcggc gacggtgctg gagccggcgg cgaccaagaa gaatttgcgc gccgccacga acggcggtgt gccgcccgac accggcatcg tgggctacta cgactacttc accaacccga ccccacacaa gtgtcgcgag acggaattca gccggcgcaa ctggagctcc ttctcccgct gcgagccctt cctccgccag ctggacaagc tctacagccg gatggccccc atgcaccacc accttcagaa ggtcgcgatc cccacgcagt accagctgtg cgacacggtg ttcagcacca tcacggtgaa ccgcaacttc cgcaccgccg tgcacaccga caagggcgac ttccgcagcg gcctcggcgt cctcgctgtt atcaacggca agttcgaggg ctgccacctc gccatcaaga aaatcaacaa ggccttcgag ctcaaaaccg gcgacgtcct tctcttcgac acctcgctgg agcacggcaa caccgaggtg accagcgcgg ccgatgactg gtaccgcaca agcgtcgtct gctacctccg caccggcctc atgtcgtcgg tgtgcgagat ggagcggcgc aagcacctca accgtctcat cgtgcagcag ctgcagagcg ccgcggtgcg ggaggccacc gtcaacatca acggcgccga cagcagcctg cccccgctct tcgtgccgac acggctggcg agccagctgg cgccggtgca gctctccgcc ctcggcttca ttgtggagcg ggcggagaag cagagcgggt gcgtggtggc catgacgatg ggccttggca agacgcttgt cgccctcacc gtctgcttct cccgcctcca cctcgcgccg cagagcgaca tcctcattct cacccccaag ccgatcatca gccactgggt ggacgaaaag aacaagtggg gtgcctacgg actgcacttc agccacttcg tcgcctcgga cggcctcaac aacctcgagt ttgagcagca gctgctcgag tacgaccggc agcgcacgaa taagcggcca cggaccgggc acgtctttgt catcaacagc gaactcctca gcggcttcct gcggcgcttt cggcacttca cgccgtcgct catcatcgtc gacgagggcc accgcgtggc gtccaaggga aacaaaatga ccgagtcgct ggagcggctg cggtgcaacc tgcgcgtcgt cctctccggc accccactgc aaaacgacgc gagcgagttg taccgcgtgg tggggtgggt gaaccgcggc gtcgcgaagg tgcttccccc gcgccgcttt caagagctgg cgaacgaggt gaaccacttt atccaaggcg acgacggcgc cttccagagc gccgtggtgg ctctcgagta catccaagac tggatgcggg gcttcgtctt ccgcgagatg gagaacgact tgccgccact gcacgactac ctgctcgtat gcggctcctc cgacgtgcag aaggagtacg aggcgaagat cggcctcacc gacggtgcca cggcgtcgct caaggcgacc gagcaccgcc cgcaccacct ctccacccac cccgcctgct acctcgcctt cgtgtccgac agctaccagt ccatgcttgt cggtcctgcg gcccgcgagc gtgagagcgc cgccaactcc cgtgtcacga gcctcgacga ggtggacacc acgcggctgg agcggtatgc ccagatggtc gagacggacc acctcgacag cttcatcgac gtcagtggga agatgcgcgt gctggtggac atcgtgcgcc gcgtgcaggc gaagcgggaa aaacttatcg tcttctccct ctacgtcggg tcgcaggact tgatccatcg cacgctgacg gcgctgcgtg tgtgcacctt caccgtccgc ggtcgcgact cgcaggaccg ccgccgccgc gcgatgcacg agttcagcaa caacgaaaac ctgatggtgc tggtggcgtc caccaaggtt gcggcttacg ggctggactt cacggcagcg aaccacgtcg tgctcttcga ctcgtggtgg aacccgcagg tggatgccca ggccatcgcc cgtgcctacc gccgcaacca gaagaagccc gtcaccgtct accggctgat ctcggccacc gaaaacaaat cggtgctgcg gttgcagacg cgcaaaatag ccctctttcg gtgcatcctg cacgagcgtc ccagtcgcgc ggcaaccgtg gacgagatag aggactgcgc cgcgacggag aaggacgagg agcgccgcgc cttctggctg aggctgaagt cctcgccgct ctccggaggc ttccccgccc tcgtcaacgt gtacaggtac caggaaagcg tccgtgagag tgtgtag
Details for CrfaA.19528.a.B3.GE41384
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD ARERRDDLTG EETVDAVHGA VSEYVSIEGL NLVTKTSIFY DREGDGQRVV AVYIRGGLPT DVCEAAATVL EPAATKKNLR AATNGGVPPD TGIVGYYDYF TNPTPHKCRE TEFSRRNWSS FSRCEPFLRQ LDKLYSRMAP MHHHLQKVAI PTQYQLCDTV FSTITVNRNF RTAVHTDKGD FRSGLGVLAV INGKFEGCHL AIKKINKAFE LKTGDVLLFD TSLEHGNTEV TSAADDWYRT SVVCYLRTGL MSSVCEMERR KHLNRLIVXQ LQS
Validated NT Sequence
atggctcacc accaccacca ccatatggac gcacgcgagc ggagggacga cctgacgggc gaggagaccg tcgacgccgt ccacggcgcc gtctcggagt acgtctcgat cgagggcctc aacctcgtga ccaagacatc catcttctac gaccgcgagg gcgacggcca gcgcgtcgtg gcggtgtaca tccgcggcgg cctcccgaca gacgtttgcg aggcggcggc gacggtgctg gagccggcgg cgaccaagaa gaatttgcgc gcagccacga acggcggtgt gccgcccgac accggcatcg tgggctacta cgactacttc accaacccga ccccacacaa gtgtcgcgag acggaattca gccggcgcaa ctggagctcc ttctcccgct gcgagccctt cctccgccag ctggacaagc tctacagccg gatggccccc atgcaccacc accttcagaa ggtcgcgatc cccacgcagt accagctgtg cgacacggtg ttcagcacca tcacggtgaa ccgcaacttc cgcaccgccg tgcacaccga caagggcgac ttccgcagcg gcctcggcgt cctcgctgtt atcaacggca agttcgaggg ctgccacctc gccatcaaga aaatcaacaa ggccttcgag ctcaaaaccg gcgacgtcct tctcttcgac acctcgctgg agcacggcaa caccgaggtg accagcgcgg ccgatgactg gtaccgcaca agcgtcgtct gctacctccg caccggcctc atgtcgtcgg tgtgcgagat ggagcggcgc aagcacctca accgtctcat cgtnnngcag ctgcagagct aagtgagtaa gat
Expected Protein Sequence
MAHHHHHHDA RERRDDLTGE ETVDAVHGAV SEYVSIEGLN LVTKTSIFYD REGDGQRVVA VYIRGGLPTD VCEAAATVLE PAATKKNLRA ATNGGVPPDT GIVGYYDYFT NPTPHKCRET EFSRRNWSSF SRCEPFLRQL DKLYSRMAPM HHHLQKVAIP TQYQLCDTVF STITVNRNFR TAVHTDKGDF RSGLGVLAVI NGKFEGCHLA IKKINKAFEL KTGDVLLFDT SLEHGNTEVT SAADDWYRTS VVCYLRTGLM SSVCEMERRK HLNRLIVQQL QS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgcac gcgagcggag ggacgacctg acgggcgagg aaaccgtcga cgccgtccac ggcgccgtct cggagtacgt ctcgatcgag ggcctcaacc tcgtgaccaa gacatccatc ttctacgacc gcgagggcga cggccagcgc gtcgtggcgg tgtacatccg cggcggcctc ccgacagacg tttgcgaggc ggcggcgacg gtgctggagc cggcggcgac caagaagaat ttgcgcgccg ccacgaacgg cggtgtgccg cccgacaccg gcatcgtggg ctactacgac tacttcacca acccgacccc acacaagtgt cgcgagacgg aattcagccg gcgcaactgg agctccttct cccgctgcga gcccttcctc cgccagctgg acaagctcta cagccggatg gcccccatgc accaccacct tcagaaggtc gcgatcccca cgcagtacca gctgtgcgac acggtgttca gcaccatcac ggtgaaccgc aacttccgca ccgccgtgca caccgacaag ggcgacttcc gcagcggcct cggcgtcctc gctgttatca acggcaagtt cgagggctgc cacctcgcca tcaagaaaat caacaaggcc ttcgagctca aaaccggcga cgtccttctc ttcgacacct cgctggagca cggcaacacc gaggtgacca gcgcggccga tgactggtac cgcacaagcg tcgtctgcta cctccgcacc ggcctcatgt cgtcggtgtg cgagatggag cggcgcaagc acctcaaccg tctcatcgtg cagcagctgc agagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat