CrfaA.19514.a

KREPA4

CENTER ID: CrfaA.19514.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.19514.a.B1.GE41066 full length 1 293
CrfaA.19514.a.B2.GE41067 truncation1 19 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CFAC1_250014300

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFWSRSLYSA SRAGAPPVMT LREATQTARR MGCLGTPPNT TPAAPSCSPS TASTPVADPL LLRRCTGADA RGSNSPSPSE ATWSGIVLRQ RQLFQNSHTP SDGGAVAVSV SAVNTIHVEG EVAAVWCGYE ASNSSGRAGD GFPNRRTVSM TTPVNRDFPT VLLWLSMEDA CGRADTSCLL AVRCRLSPEV VELRWRAQQQ AAAANADGDA EKSSAVEEAG VDARLRCVKA WASTALLHRR VVVSGQLRVS EVYDGDLDAV VAVPVVELPQ DNLLGSVMVL RSEKVKLSKV SQF
NT Sequence
ATGTTCTGGT CTCGGTCCCT GTACAGTGCG AGCCGCGCTG GGGCACCTCC AGTCATGACG TTGCGAGAGG CGACACAGAC GGCACGCAGG ATGGGTTGCC TCGGCACCCC ACCCAACACC ACGCCTGCTG CTCCTTCGTG CTCTCCATCG ACAGCGTCGA CACCAGTTGC AGATCCCCTG TTGCTGCGAC GGTGCACCGG CGCGGACGCG CGAGGCAGCA ACTCCCCTTC CCCTTCCGAA GCTACATGGA GCGGCATCGT GCTGCGGCAG CGGCAGCTCT TCCAGAACTC GCACACCCCA AGCGACGGCG GTGCCGTTGC CGTGAGCGTG TCGGCGGTCA ACACGATCCA CGTGGAGGGT GAGGTGGCCG CTGTCTGGTG TGGCTACGAG GCGTCCAACA GCTCGGGGAG GGCCGGAGAC GGCTTCCCGA ACCGCCGCAC GGTTTCCATG ACGACACCAG TGAACAGGGA CTTCCCAACG GTGCTGCTGT GGCTGAGCAT GGAGGATGCG TGCGGCAGGG CGGATACATC CTGTCTGCTG GCGGTGCGCT GTCGTCTGTC GCCGGAGGTG GTGGAGCTGC GGTGGCGTGC ACAGCAGCAG GCCGCTGCAG CGAACGCAGA CGGGGATGCT GAGAAGAGCA GTGCGGTTGA AGAGGCAGGT GTCGATGCGA GGCTGCGGTG CGTGAAGGCG TGGGCATCGA CGGCGCTGCT GCACCGACGC GTCGTTGTCA GCGGGCAGCT GCGCGTCTCT GAGGTGTACG ACGGCGACCT TGATGCCGTG GTGGCCGTGC CAGTGGTGGA GCTGCCGCAG GACAACTTGC TGGGCAGCGT GATGGTGCTG CGCTCAGAGA AGGTGAAGCT GTCGAAGGTG TCGCAATTTT GA
Details for CrfaA.19514.a.B1.GE41066
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF WSRSLYSASR AGAPPVMTLR EATQTARRMG CLGTPPNTTP AAPSCSPSTA STPVADPLLL RRCTGADARG SNSPSPSEAT WSGIVLRQRQ LFQNSHTPSD GGAVAVSVSA VNTIHVEGEV AAVWCGYEAS NSAGRAGDGF PNRRTVSMTT PVNRDFPTVL LWLSMEDASG RADTSCLLAV RCRLSPEVVE LRWRAQQQAA AANADGDAEK SSAVEEAGVD ARLRCVKAWA STALLHRRVV VSGQLRVSEV YDGDLDAVVA VPVVELPQDN LLGSVMVLRS EKVXXXKVSQ F
Validated NT Sequence
atcttactca cttaaaattg cgacaccttn nnnnnnntca ccttctctga gcgcagcacc atcacgctgc ccagcaagtt gtcctgcggc agctccacca ctggcacggc caccacggca tcaaggtcgc cgtcgtacac ctcagagacg cgcagctgcc cgctgacaac gacgcgtcgg tgcagcagcg ccgtcgatgc ccacgccttc acgcaccgca gcctcgcatc gacacctgcc tcttcaaccg cactgctctt ctcagcatcc ccgtctgcgt tcgctgcagc ggcctgctgc tgtgcacgcc accgcagctc caccacctcc ggcgacagac gacagcgcac cgccagcaga caggatgtat ccgccctgcc gctcgcatcc tccatgctca gccacagcag caccgtcggg aagtccctgt tcaccggcgt tgtcatggaa acggtgcggc ggttcgggaa gccgtcgccg gccctccccg cactgttgga cgcctcgtag ccacaccaga cggcggccac ctcaccctcc acgtggatcg tgttgaccgc cgacacgctc acggcaacgg caccgccgtc gcttggggtg tgcgagttct ggaagagctg ccgctgccgc agcacgatgc cgctccatgt agcttcggaa ggggaagggg agttgctgcc tcgcgcgtcc gcgccggtgc accgtcgcag caacagggga tctgcaactg gtgtcgacgc tgtcgatgga gagcacgaag gagcagcagg cgtggtgttg ggtggggtgc cgaggcaacc cattctgcgt gccgtctgcg tcgcctctcg caacgtcatg actggaggtg ccccagcgcg gctcgcactg tacagggacc gagaccagaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMF WSRSLYSASR AGAPPVMTLR EATQTARRMG CLGTPPNTTP AAPSCSPSTA STPVADPLLL RRCTGADARG SNSPSPSEAT WSGIVLRQRQ LFQNSHTPSD GGAVAVSVSA VNTIHVEGEV AAVWCGYEAS NSSGRAGDGF PNRRTVSMTT PVNRDFPTVL LWLSMEDACG RADTSCLLAV RCRLSPEVVE LRWRAQQQAA AANADGDAEK SSAVEEAGVD ARLRCVKAWA STALLHRRVV VSGQLRVSEV YDGDLDAVVA VPVVELPQDN LLGSVMVLRS EKVKLSKVSQ F
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttct ggtctcggtc cctgtacagt gcgagccgcg ctggggcacc tccagtcatg acgttgcgag aggcgacaca gacggcacgc aggatgggtt gcctcggcac cccacccaac accacgcctg ctgctccttc gtgctctcca tcgacagcgt cgacaccagt tgcagatccc ctgttgctgc gacggtgcac cggcgcggac gcgcgaggca gcaactcccc ttccccttcc gaagctacat ggagcggcat cgtgctgcgg cagcggcagc tcttccagaa ctcgcacacc ccaagcgacg gcggtgccgt tgccgtgagc gtgtcggcgg tcaacacgat ccacgtggag ggtgaggtgg ccgctgtctg gtgtggctac gaggcgtcca acagctcggg gagggccgga gacggcttcc cgaaccgccg cacggtttcc atgacgacac cagtgaacag ggacttccca acggtgctgc tgtggctgag catggaggat gcgtgcggca gggcggatac atcctgtctg ctggcggtgc gctgtcgtct gtcgccggag gtggtggagc tgcggtggcg tgcacagcag caggccgctg cagcgaacgc agacggggat gctgagaaga gcagtgcggt tgaagaggca ggtgtcgatg cgaggctgcg gtgcgtgaag gcgtgggcat cgacggcgct gctgcaccga cgcgtcgttg tcagcgggca gctgcgcgtc tctgaggtgt acgacggcga ccttgatgcc gtggtggccg tgccagtggt ggagctgccg caggacaact tgctgggcag cgtgatggtg ctgcgctcag agaaggtgaa gctgtcgaag gtgtcgcaat tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for CrfaA.19514.a.B2.GE41067
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT LREATQTARR MGCLGTPPNT TPAAPSCSPS TASTPVADPL LLRRCTGADA RGSNSPSPSE ATWSGIVLRQ RQLFQNSHTP SDGGAVAVSV SAVNTIHVEG EVAAVWCGYE ASNSAGRAGD GFPNRRTVSM TTPVNRDFPT VLLWLSMEDA SGRADTSCLL AVRCRLSPEV VELRWRAQQQ AAAANADGDA EKSSAVEEAG VDARLRCVKA WASTALLHRR VVVSGQLRVS EVYDGDLDAV VAVPVVELPQ DNLLGSVMVL RSEKVKXXKV SQF
Validated NT Sequence
ctcacttaaa attgcgacac cttnnnnnnc ttcaccttct ctgagcgcag caccatcacg ctgcccagca agttgtcctg cggcagctcc accactggca cggccaccac ggcatcaagg tcgccgtcgt acacctcaga gacgcgcagc tgcccgctga caacgacgcg tcggtgcagc agcgccgtcg atgcccacgc cttcacgcac cgcagcctcg catcgacacc tgcctcttca accgcactgc tcttctcagc atccccgtct gcgttcgctg cagcggcctg ctgctgtgca cgccaccgca gctccaccac ctccggcgac agacgacagc gcaccgccag cagacaggat gtatccgccc tgccgctcgc atcctccatg ctcagccaca gcagcaccgt cgggaagtcc ctgttcaccg gcgttgtcat ggaaacggtg cggcggttcg ggaagccgtc gccggccctc cccgcactgt tggacgcctc gtagccacac cagacggcgg ccacctcacc ctccacgtgg atcgtgttga ccgccgacac gctcacggca acggcaccgc cgtcgcttgg ggtgtgcgag ttctggaaga gctgccgctg ccgcagcacg atgccgctcc atgtagcttc ggaaggggaa ggggagttgc tgcctcgcgc gtccgcgccg gtgcaccgtc gcagcaacag gggatctgca actggtgtcg acgctgtcga tggagagcac gaaggagcag caggcgtggt gttgggtggg gtgccgaggc aacccattct gcgtgccgtc tgcgtcgcct ctcgcaacgt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT LREATQTARR MGCLGTPPNT TPAAPSCSPS TASTPVADPL LLRRCTGADA RGSNSPSPSE ATWSGIVLRQ RQLFQNSHTP SDGGAVAVSV SAVNTIHVEG EVAAVWCGYE ASNSSGRAGD GFPNRRTVSM TTPVNRDFPT VLLWLSMEDA CGRADTSCLL AVRCRLSPEV VELRWRAQQQ AAAANADGDA EKSSAVEEAG VDARLRCVKA WASTALLHRR VVVSGQLRVS EVYDGDLDAV VAVPVVELPQ DNLLGSVMVL RSEKVKLSKV SQF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgt tgcgagaggc gacacagacg gcacgcagga tgggttgcct cggcacccca cccaacacca cgcctgctgc tccttcgtgc tctccatcga cagcgtcgac accagttgca gatcccctgt tgctgcgacg gtgcaccggc gcggacgcgc gaggcagcaa ctccccttcc ccttccgaag ctacatggag cggcatcgtg ctgcggcagc ggcagctctt ccagaactcg cacaccccaa gcgacggcgg tgccgttgcc gtgagcgtgt cggcggtcaa cacgatccac gtggagggtg aggtggccgc tgtctggtgt ggctacgagg cgtccaacag ctcggggagg gccggagacg gcttcccgaa ccgccgcacg gtttccatga cgacaccagt gaacagggac ttcccaacgg tgctgctgtg gctgagcatg gaggatgcgt gcggcagggc ggatacatcc tgtctgctgg cggtgcgctg tcgtctgtcg ccggaggtgg tggagctgcg gtggcgtgca cagcagcagg ccgctgcagc gaacgcagac ggggatgctg agaagagcag tgcggttgaa gaggcaggtg tcgatgcgag gctgcggtgc gtgaaggcgt gggcatcgac ggcgctgctg caccgacgcg tcgttgtcag cgggcagctg cgcgtctctg aggtgtacga cggcgacctt gatgccgtgg tggccgtgcc agtggtggag ctgccgcagg acaacttgct gggcagcgtg atggtgctgc gctcagagaa ggtgaagctg tcgaaggtgt cgcaattttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat