CrfaA.19513.a

KREN1

CENTER ID: CrfaA.19513.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.19513.a.B1.GE41065 full length 1 966
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CFAC1_180016200

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLTSSPALCY ARRLRTSSHL ISPAHHTALQ RSHRCEKDAS AHGARRHQRK WSGPPAWTLL GGGLLQTTRI ARALLPVTQP PTNTSGSSSS SSSSNSTTPF FAPLASVHGR PLSSSEATPP SSVIHVQPHR LPTDLLDDMP VYKPDDSNGP YLTTLNSYYH MHSLPGALAY RFYFRHTTGA DAVDGRAAGE TTQQLRQDQG GRGESRTGRR RETPDGRWTC RLCREEISTD YSMRLHITLA SIGNRTCHPV REVALDTIAL LGVRGYPVDD IMTVWAETLF RCPDFPRLRH LSDPRWTIEK RAEALGNVLF ALRQCGIIDV SLERQLTESA AQPGNDGKLL RRRRIGFERL EYIGDNFWSV NLSKRLVQLY PDQRWLRGEH SLSFNAIRDA CEMNTNLDFV FDTLRIADLL STTEHLGVKK MKADLVEAIL GELHMHAEAY EPRLTDSEAY VEVNGVREAQ VCAVVQHCLS ELYDLVVLQH ARDLAATAIP LAKELVARRL WLPVRPFLLL TKRTQRLVER GPVQRSSSGS SSSNAETGGM RAPSTSLRPP IGTKVEDATN FFTLLPSPGT EMAAIEEMGD LSPSSLQSTA PPGTDADSEN NTDCAATPAA EEDASDLSDA SAALTAWQRS NARVLPGLPR LFDSPRVPPR IIPHPLRHLP PSDIPAATYC EHTHDEVLEP LMASYRRLKL ISDDAGQTRY VVDRRPPQWS DLISSLAPQV VPYNSPTETH VLGSSQDSGV SEEGEGEYDE ESHAGMADDS EVDGAAGTRS TALSPVFGEL QSGEVTRRLL ASIEEGNIFC RDVFYNLAAS PQPEAQTGTS PAAARTTTTS ALEWVLHNSG LPTLTPKPTE AVDGSNGKGV ADGGVFASFA ESHLVPPNTR PPPAGVVTER NVCKGVFAYL AFGEADPVGR ARQSVSEGRR DADAAAPSQA EEDGGEEERA KQVEEAVTYW KRRPFRMTDD ASLVAS
NT Sequence
ATGCTCACAT CATCCCCAGC GCTATGCTAT GCCCGTCGCC TCCGCACCTC CTCCCACTTA ATTTCTCCTG CGCATCACAC AGCGCTACAA CGCTCCCACC GCTGTGAGAA GGATGCCAGT GCGCATGGCG CACGAAGACA CCAACGGAAG TGGTCCGGGC CGCCAGCGTG GACGCTTCTC GGCGGGGGTC TCCTGCAGAC GACACGCATT GCTCGCGCGC TTCTGCCGGT GACGCAGCCC CCAACAAACA CCAGCGGCAG CAGCAGCAGC AGCAGTAGCA GCAACAGTAC AACCCCGTTT TTCGCCCCGC TTGCCAGCGT GCATGGTCGT CCCCTATCTT CCTCGGAGGC AACTCCTCCT TCGTCAGTCA TCCACGTGCA GCCCCACCGC CTACCAACCG ACTTGCTGGA TGACATGCCT GTCTACAAAC CGGACGACTC AAACGGACCG TACCTGACCA CTCTCAACAG CTACTACCAC ATGCACTCCC TGCCTGGTGC TCTTGCCTAC CGCTTTTACT TTCGCCACAC CACCGGTGCC GATGCCGTCG ACGGCAGAGC CGCTGGGGAG ACGACACAGC AGCTGCGCCA AGACCAAGGA GGCAGGGGTG AGAGTCGTAC CGGCCGCCGC CGGGAGACGC CCGACGGCCG GTGGACCTGC CGGCTGTGCC GCGAAGAGAT CAGCACCGAC TACTCCATGC GGCTGCACAT CACCTTGGCC AGCATCGGGA ATCGCACCTG CCACCCGGTG CGGGAGGTCG CGCTCGACAC AATCGCCCTC CTCGGCGTCC GCGGCTACCC GGTGGATGAC ATCATGACCG TGTGGGCCGA GACACTCTTT CGGTGCCCCG ACTTCCCGCG GCTGCGCCAC CTCTCCGACC CGCGGTGGAC CATCGAGAAG CGGGCGGAGG CGTTGGGGAA CGTCTTGTTC GCACTCCGTC AGTGCGGCAT CATCGACGTC TCGCTAGAGC GCCAGCTGAC GGAGAGCGCG GCGCAACCGG GCAACGACGG CAAGCTGCTC CGTCGCCGCC GCATCGGGTT TGAGCGGCTG GAGTACATCG GTGACAACTT CTGGAGCGTG AATCTGTCGA AGCGGCTTGT GCAGCTCTAC CCCGATCAGC GCTGGCTGCG CGGCGAGCAC TCCCTCTCCT TCAACGCGAT TCGTGACGCC TGTGAGATGA ACACCAACCT CGACTTTGTC TTCGACACGC TGCGCATTGC CGATCTCCTC TCCACCACCG AGCACCTGGG CGTCAAGAAG ATGAAGGCCG ACCTTGTTGA GGCCATCTTA GGTGAGCTGC ACATGCACGC GGAGGCCTAC GAGCCGCGGC TGACCGACTC GGAGGCCTAC GTCGAGGTGA ACGGCGTGCG TGAGGCGCAG GTGTGTGCGG TGGTGCAGCA CTGCCTCTCC GAGCTGTACG ACCTCGTTGT TCTCCAGCAC GCGCGGGACC TGGCGGCGAC GGCGATTCCG CTGGCGAAGG AGCTCGTGGC GCGCCGCCTG TGGCTGCCCG TGCGGCCGTT CCTCCTCCTC ACCAAGCGAA CGCAACGCCT TGTGGAGCGC GGCCCGGTTC AGCGCAGCAG CAGCGGTAGT AGTAGTAGTA ATGCGGAGAC CGGAGGAATG CGCGCGCCGT CAACGAGTTT GCGGCCACCG ATAGGGACCA AGGTAGAGGA TGCGACGAAC TTCTTCACGC TACTGCCGTC TCCTGGGACG GAGATGGCCG CCATTGAGGA AATGGGCGAT CTCTCGCCTT CCTCTCTTCA ATCTACTGCT CCTCCCGGCA CGGATGCAGA TAGCGAGAAC AACACAGACT GCGCTGCGAC GCCGGCAGCG GAAGAGGACG CATCGGACTT GAGTGATGCC TCGGCGGCTC TCACGGCGTG GCAGCGCAGC AATGCACGTG TGCTGCCCGG CCTCCCGCGT CTCTTCGACT CTCCGCGTGT GCCTCCGCGC ATTATTCCCC ACCCGCTGCG CCACCTTCCC CCCTCCGACA TCCCAGCGGC TACCTACTGC GAGCACACCC ACGATGAAGT CCTCGAGCCC CTCATGGCGA GTTATCGTCG CCTGAAGCTC ATCAGCGATG ACGCCGGGCA GACGCGGTAT GTCGTGGATC GTCGTCCCCC TCAGTGGAGC GATCTGATTT CCAGCCTGGC GCCGCAGGTC GTGCCATACA ACTCACCAAC GGAGACACAC GTCCTTGGCA GTAGTCAAGA CAGTGGTGTC AGTGAAGAGG GGGAGGGGGA GTATGATGAG GAGTCTCACG CTGGGATGGC GGACGACAGC GAGGTGGATG GCGCAGCTGG CACCCGCAGC ACGGCGCTCA GCCCAGTCTT TGGCGAGCTG CAGAGCGGAG AGGTAACGCG TCGGCTGCTG GCCTCCATAG AGGAAGGTAA TATCTTCTGT CGCGATGTGT TCTACAACTT GGCGGCATCA CCACAGCCAG AGGCCCAAAC CGGCACCTCA CCAGCAGCTG CAAGGACAAC AACAACGAGT GCGCTGGAGT GGGTTCTGCA CAACTCTGGT CTGCCCACTC TCACGCCGAA GCCCACCGAG GCTGTGGACG GCAGCAATGG CAAGGGTGTC GCCGATGGCG GTGTCTTCGC CTCCTTCGCC GAGTCGCACC TTGTACCACC CAACACGCGT CCCCCACCCG CTGGTGTGGT GACGGAGCGC AACGTCTGCA AGGGCGTCTT CGCCTACCTC GCCTTCGGAG AGGCAGACCC GGTCGGGCGG GCGAGGCAGT CAGTGAGTGA GGGGAGGAGA GATGCCGACG CAGCAGCGCC ATCCCAGGCG GAAGAGGACG GAGGCGAGGA GGAGAGGGCG AAGCAGGTGG AGGAGGCGGT GACGTATTGG AAGCGGCGTC CGTTTCGCAT GACAGATGAT GCGTCGTTGG TGGCGAGCTG A
Details for CrfaA.19513.a.B1.GE41065
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 107kDa
OBSERVED MW: 107kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML TSSPALCYAR RLRTSSHLIS PAHHTALQRS HRCEKDASAH GARRHQRKWS GPPAWTLLGG GLLQTTRIAR ALLPVTQPPT NTSGSSSSSS SSNSTTPFFA PLASVHGRPL SSSEATPPSS VIHVQPHRLP TDLLDDMPVY KPDDSNGPYL TTLNSYYHMH SLPGALAYRF YFRHTTGADA VDGRAAGETT QQLRQDQGGR GESRTGRRRE TPDGRWTCRL CREEISTDYS MRLHITLASI GNRTCHPVRE VALDTIALLG VRGYPVDDIM TVWAETLFRC PDFPRLRHLS DPRWTIEKRA EALGNVLFAL RQCGIIDVSL ERQLTESAAQ PGNDGKLLRR RRIGFERLEY IGDNFWSVNL SKRLVQLYPD QRWLRGEHSL SFNAIRDACE MNTNLDFVFD TLRIADLLST TEHLGVKKMK ADLVEAILGE LHMHAEAYEP RLTDSEAYVE VNGVREAQVC AVVQHCLSEL YDLVVLQHAR DLAATAIPLA KELVARRLWL PVRPFLLLTK RTQRLVERGP VQRSSSGSSS SNAETGGMRA PSTSLRPPIG TKVEDATNFF TLLPSPGTEM AAIEEMGDLS PSSLQSTAPP GTDADSENNT DCAATPAAEE DASDLSDASA ALTAWQRSNA RVLPGLPRLF DSPRVPPRII PHPLRHLPPS DIPAATYCEH THDEVLEPLM ASYRRLKLIS DDAGQTRYVV DRRPPQWSDL ISSLAPQVVP YNSPTETHVL GSSQDSGVSE EGEGEYDEES HAGMADDSEV DGAAGTRSTA LSPVFGELQS GEVTRRLLAS IEEGNIFCRD VFYNLAASPQ PEAQTGTSPA AARTTTTSAL EWVLHNSGLP TLTPKPTEAV DGSNGKGVAD GGVFASFAES HLVPPNTRPP PAGVVTERNV CKGVFAYLAF GEADPVGRAR QSVSEGRRDA DAAAPSQAEE DGGEEERAKQ VEEAVTYWKR RPFRMTDDAS LVAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca catcatcccc agcgctatgc tatgcccgtc gcctccgcac ctcctcccac ttaatttctc ctgcgcatca cacagcgcta caacgctccc accgctgtga gaaggatgcc agtgcgcatg gcgcacgaag acaccaacgg aagtggtccg ggccgccagc gtggacgctt ctcggcgggg gtctcctgca gacgacacgc attgctcgcg cgcttctgcc ggtgacgcag cccccaacaa acaccagcgg cagcagcagc agcagcagta gcagcaacag tacaaccccg tttttcgccc cgcttgccag cgtgcatggt cgtcccctat cttcctcgga ggcaactcct ccttcgtcag tcatccacgt gcagccccac cgcctaccaa ccgacttgct ggatgacatg cctgtctaca aaccggacga ctcaaacgga ccgtacctga ccactctcaa cagctactac cacatgcact ccctgcctgg tgctcttgcc taccgctttt actttcgcca caccaccggt gccgatgccg tcgacggcag agccgctggg gagacgacac agcagctgcg ccaagaccaa ggaggcaggg gtgagagtcg taccggccgc cgccgggaga cgcccgacgg ccggtggacc tgccggctgt gccgcgaaga gatcagcacc gactactcca tgcggctgca catcaccttg gccagcatcg ggaatcgcac ctgccacccg gtgcgggagg tcgcgctcga cacaatcgcc ctcctcggcg tccgcggcta cccggtggat gacatcatga ccgtgtgggc cgagacactc tttcggtgcc ccgacttccc gcggctgcgc cacctctccg acccgcggtg gaccatcgag aagcgggcgg aggcgttggg gaacgtcttg ttcgcactcc gtcagtgcgg catcatcgac gtctcgctag agcgccagct gacggagagc gcggcgcaac cgggcaacga cggcaagctg ctccgtcgcc gccgcatcgg gtttgagcgg ctggagtaca tcggtgacaa cttctggagc gtgaatctgt cgaagcggct tgtgcagctc taccccgatc agcgctggct gcgcggcgag cactccctct ccttcaacgc gattcgtgac gcctgtgaga tgaacaccaa cctcgacttt gtcttcgaca cgctgcgcat tgccgatctc ctctccacca ccgagcacct gggcgtcaag aagatgaagg ccgaccttgt tgaggccatc ttaggtgagc tgcacatgca cgcggaggcc tacgagccgc ggctgaccga ctcggaggcc tacgtcgagg tgaacggcgt gcgtgaggcg caggtgtgtg cggtggtgca gcactgcctc tccgagctgt acgacctcgt tgttctccag cacgcgcggg acctggcggc gacggcgatt ccgctggcga aggagctcgt ggcgcgccgc ctgtggctgc ccgtgcggcc gttcctcctc ctcaccaagc gaacgcaacg ccttgtggag cgcggcccgg ttcagcgcag cagcagcggt agtagtagta gtaatgcgga gaccggagga atgcgcgcgc cgtcaacgag tttgcggcca ccgataggga ccaaggtaga ggatgcgacg aacttcttca cgctactgcc gtctcctggg acggagatgg ccgccattga ggaaatgggc gatctctcgc cttcctctct tcaatctact gctcctcccg gcacggatgc agatagcgag aacaacacag actgcgctgc gacgccggca gcggaagagg acgcatcgga cttgagtgat gcctcggcgg ctctcacggc gtggcagcgc agcaatgcac gtgtgctgcc cggcctcccg cgtctcttcg actctccgcg tgtgcctccg cgcattattc cccacccgct gcgccacctt cccccctccg acatcccagc ggctacctac tgcgagcaca cccacgatga agtcctcgag cccctcatgg cgagttatcg tcgcctgaag ctcatcagcg atgacgccgg gcagacgcgg tatgtcgtgg atcgtcgtcc ccctcagtgg agcgatctga tttccagcct ggcgccgcag gtcgtgccat acaactcacc aacggagaca cacgtccttg gcagtagtca agacagtggt gtcagtgaag agggggaggg ggagtatgat gaggagtctc acgctgggat ggcggacgac agcgaggtgg atggcgcagc tggcacccgc agcacggcgc tcagcccagt ctttggcgag ctgcagagcg gagaggtaac gcgtcggctg ctggcctcca tagaggaagg taatatcttc tgtcgcgatg tgttctacaa cttggcggca tcaccacagc cagaggccca aaccggcacc tcaccagcag ctgcaaggac aacaacaacg agtgcgctgg agtgggttct gcacaactct ggtctgccca ctctcacgcc gaagcccacc gaggctgtgg acggcagcaa tggcaagggt gtcgccgatg gcggtgtctt cgcctccttc gccgagtcgc accttgtacc acccaacacg cgtcccccac ccgctggtgt ggtgacggag cgcaacgtct gcaagggcgt cttcgcctac ctcgccttcg gagaggcaga cccggtcggg cgggcgaggc agtcagtgag tgaggggagg agagatgccg acgcagcagc gccatcccag gcggaagagg acggaggcga ggaggagagg gcgaagcagg tggaggaggc ggtgacgtat tggaagcggc gtccgtttcg catgacagat gatgcgtcgt tggtggcgag ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat