CrfaA.19512.a

KREX2

CENTER ID: CrfaA.19512.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrfaA.19512.a.B1.GE40826 full length 1 1077
CrfaA.19512.a.B2.GE40827 5'-3' exonuclease 39 681
CrfaA.19512.a.B3.GE40828 endo-exonuclease-phosphatase 734 1077
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CFAC1_060012800

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYARSRLFLY VSVRGSGSGP ASSTAAGGSS SSSSSGRRDY MTSEGIADAF HASHHRAAWT FDHVLVDIPS IINSVAFMAR DLVGHERDKE TVRNAHRQLQ TIILRRLQPR KSLALLFDGS EPLWMLEKTR LFPGRRYDSK FYRSCASPMP HLLEEKLRST AMELRTPPWE TVISGPATPG LSEGKMSAYL LDLATRMLRP PAQPTGLCPA VTAGDTVCVV GAPELAWMGL GVTPFQNITT VTLQQGELTS CSVQETMAWL RLGHLTGPTD GAATTTAVAG AEGESSVDLA QQQQQQQQQQ RRLSAARTDV VFLYLLTHGH SATGLPQVLS TPFADVLDAY IALEAENAQR TLDASPPLSS GEEDGVAPLL RFRSILFDEE PITLASLSRP SLRLRVASLQ KVLVRVYNAV SSGGSGGGAN LPASSKPVPH VATLLTWTLQ AHGLLCSGGV PSPDWTPTPD IGAGNSSSGE NAAAVAEGGA GGTVAVAAAT AMLDKYPKVS LEMLIQHLGY LVNTNAAAAG AGSQETHFCP PRSRSFGLTA VEALLMTATQ PDQIHQILPL YARGHSLSDA AAKDITGTRN VHEALRKTQQ AVGGAVAQAE TELTTLAANP PRVRDVNAVA AGLHPALAHL PSHLFVRAMG SQGPPPGWSY YGIHLGIKAE AMNVRYCLNA GDTATPRVID AGVLSQPDTL FVWAARGSEG EGTWTAVPCA AAGSSSAAAA ASTPSGEADA AAAVSTLRVL TWNSQFDRHS KQETPLGRAG IDWCTATRYI AASKVLAAAE ADVICLQEVE PAWVSFLCAQ SWVREAYALS AVDISPALHP HGVLLLVRRG VGVVKRVRHV NLPATAGYVA MMPELMLQVG REVAPVVVGG LHLVAPYTQR NENDRVAQLA KLESHLTQQM ERGWEPATAA AAAADAVDAD AAARGEKPAS GSAASTEEEK EDVSYLLIGD FNDYPTRPYR PPAALAYKDA WELLNPNCHR STATAAERGY TIDGAANPYT AKIIEPKFFG RPDRILFRSK QLQPIDAELV GTATVMEVLP KPAGGGPLMK VNNPAEAVPD YLYPSDHFGV LVEFQLV
NT Sequence
ATGTACGCGA GAAGTCGCCT CTTTCTGTAC GTTAGCGTCC GGGGCAGCGG CAGCGGCCCC GCATCGTCCA CAGCAGCGGG AGGCAGCAGC AGCAGCAGCA GCAGCGGCAG GAGAGACTAC ATGACCTCCG AGGGCATTGC CGACGCCTTT CATGCATCCC ACCACCGCGC TGCGTGGACC TTCGACCACG TCCTTGTCGA CATCCCCTCC ATCATCAACT CGGTCGCCTT CATGGCTCGT GATCTTGTTG GGCACGAGCG GGACAAGGAA ACGGTGCGCA ACGCACACCG GCAGCTGCAG ACGATTATTC TGCGCCGTCT GCAACCCCGT AAGAGTCTCG CTCTTCTCTT CGACGGCAGT GAGCCGCTGT GGATGCTGGA GAAGACACGT CTGTTTCCTG GTCGACGATA CGACTCAAAG TTTTACCGGA GCTGTGCCTC ACCGATGCCG CACCTGCTGG AGGAGAAGTT GCGGAGCACT GCGATGGAGT TGCGGACGCC GCCGTGGGAG ACAGTCATCA GCGGTCCCGC CACGCCGGGG CTGTCGGAGG GAAAGATGTC AGCCTACCTG CTGGACCTGG CCACCCGCAT GCTGCGTCCA CCCGCGCAGC CCACCGGCCT CTGCCCTGCT GTGACGGCGG GTGATACGGT GTGTGTGGTC GGGGCCCCGG AGCTGGCATG GATGGGGCTT GGCGTGACGC CCTTCCAGAA CATCACGACG GTGACCCTGC AGCAGGGCGA GCTGACGAGC TGCTCGGTGC AGGAGACGAT GGCGTGGCTG CGGCTGGGGC ACTTGACGGG CCCTACAGAC GGCGCAGCGA CGACGACTGC TGTCGCTGGT GCGGAGGGAG AGAGCAGCGT GGACCTTGCC CAGCAACAGC AGCAGCAGCA GCAACAGCAG CGGCGGCTCT CTGCTGCTCG CACAGATGTT GTCTTTCTCT ATCTCTTAAC CCACGGTCAC AGCGCAACAG GGCTGCCGCA GGTGCTGTCG ACGCCGTTTG CCGATGTGCT GGACGCCTAC ATCGCGCTGG AGGCGGAGAA CGCTCAACGG ACACTCGATG CATCACCACC GCTGTCGAGC GGTGAAGAAG ACGGCGTGGC GCCGCTGCTC CGATTTCGCA GCATCCTCTT CGATGAAGAG CCCATCACCT TGGCAAGTCT CAGCCGCCCA TCGCTGCGTC TGCGCGTGGC ATCGTTGCAG AAAGTACTGG TGCGGGTGTA CAACGCCGTT TCAAGCGGCG GCAGCGGTGG CGGTGCCAAC TTGCCAGCGA GCTCGAAGCC TGTGCCACAC GTGGCGACCT TGTTGACCTG GACGCTGCAG GCGCACGGGC TGCTGTGCTC CGGTGGTGTG CCCTCGCCGG ATTGGACCCC CACGCCAGAC ATCGGTGCGG GCAACAGCAG CAGCGGCGAG AATGCGGCGG CGGTGGCAGA GGGCGGTGCA GGGGGGACGG TCGCTGTTGC CGCTGCGACG GCGATGCTGG ATAAATACCC AAAGGTGTCT CTTGAGATGC TCATTCAGCA CCTCGGCTAC CTCGTCAACA CCAACGCTGC CGCTGCTGGA GCTGGCAGCC AAGAGACCCA CTTCTGCCCC CCACGCTCTC GAAGCTTCGG CCTCACAGCT GTGGAGGCGC TACTCATGAC GGCGACCCAG CCCGACCAGA TCCATCAGAT TTTGCCCCTC TACGCACGGG GTCACTCTCT CTCTGACGCC GCTGCCAAGG ACATCACCGG CACCCGCAAC GTGCACGAGG CGCTGCGCAA GACACAGCAG GCCGTCGGCG GCGCGGTGGC GCAGGCGGAG ACGGAGCTGA CGACGCTGGC GGCGAACCCG CCCCGCGTGC GGGATGTCAA CGCCGTCGCT GCCGGCCTCC ACCCCGCCCT CGCCCACCTC CCGTCTCACC TCTTCGTGCG CGCGATGGGC AGCCAGGGCC CGCCGCCGGG CTGGAGCTAC TACGGCATCC ATCTCGGCAT CAAGGCCGAG GCGATGAACG TGCGCTACTG CCTGAACGCC GGCGACACGG CGACGCCGCG GGTGATCGAT GCCGGGGTGC TGAGCCAGCC GGACACGCTC TTTGTGTGGG CTGCGCGGGG CAGCGAGGGA GAGGGGACGT GGACAGCGGT GCCCTGTGCT GCTGCTGGCT CCTCTTCAGC AGCTGCAGCA GCATCAACGC CGTCGGGAGA AGCCGATGCA GCGGCGGCGG TGTCAACGCT GCGGGTTCTG ACGTGGAATA GTCAGTTTGA TCGTCACAGC AAGCAGGAGA CACCGCTTGG TCGGGCTGGC ATTGACTGGT GCACCGCCAC GCGCTACATT GCTGCTTCGA AGGTGTTGGC GGCAGCAGAG GCCGACGTCA TTTGCCTGCA GGAAGTCGAA CCCGCGTGGG TGAGCTTCCT CTGTGCCCAG TCGTGGGTGC GGGAGGCGTA CGCGCTCAGT GCTGTCGACA TCAGCCCCGC TCTGCACCCG CACGGCGTTC TTCTTCTCGT ACGCCGCGGT GTCGGTGTGG TGAAGCGCGT GCGCCACGTG AACCTCCCGG CTACTGCGGG GTACGTTGCG ATGATGCCGG AGCTGATGCT GCAGGTGGGT CGCGAGGTAG CTCCGGTGGT TGTCGGTGGC CTTCACCTTG TGGCGCCGTA CACGCAGAGA AACGAGAACG ATCGTGTGGC GCAGCTCGCG AAGCTGGAGA GCCACCTGAC GCAACAGATG GAGCGTGGGT GGGAACCTGC GACAGCCGCA GCGGCAGCAG CAGATGCAGT AGATGCAGAT GCAGCAGCGA GGGGGGAAAA ACCTGCAAGC GGGAGTGCGG CGAGCACCGA AGAAGAAAAA GAAGACGTCT CCTACCTGCT TATTGGCGAC TTCAACGACT ACCCGACGAG GCCATACCGT CCACCCGCAG CGCTGGCGTA CAAGGACGCG TGGGAGCTGC TGAACCCCAA CTGCCACCGC AGCACCGCCA CGGCTGCTGA GCGTGGCTAC ACGATCGACG GCGCCGCAAA CCCGTACACG GCGAAGATCA TTGAGCCGAA GTTCTTTGGC CGGCCCGATC GCATTCTGTT CCGCTCGAAG CAGCTCCAAC CCATCGATGC GGAGCTGGTT GGGACGGCGA CGGTGATGGA GGTGCTGCCC AAGCCAGCGG GTGGAGGGCC GCTGATGAAG GTGAACAACC CGGCCGAGGC GGTGCCAGAC TATCTCTACC CATCCGACCA CTTCGGTGTA CTGGTCGAGT TCCAGTTGGT GTAA
Details for CrfaA.19512.a.B1.GE40826
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 115kDa
OBSERVED MW: 115kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMY ARSRLFLYVS VRGSGSGPAS STAAGGSSSS SSSGRRDYMT SEGIADAFHA SHHRAAWTFD HVLVDIPSII NSVAFMARDL VGHERDKETV RNAHRQLQTI ILRRLQPRKS LALLFDGSEP LWMLEKTRLF PGRRYDSKFY RSCASPMPHL LEEKLRSTAM ELRTPPWETV ISGPATPGLS EGKMSAYLLD LATRMLRPPA QPTGLCPAVT AGDTVCVVGA PELAWMGLGV TPFQNITTVT LQQGELTSCS VQETMAWLRL GHLTGPTDGA ATTTAVAGAE GESSVDLAQQ QQQQQQQQRR LSAARTDVVF LYLLTHGHSA TGLPQVLSTP FADVLDAYIA LEAENAQRTL DASPPLSSGE EDGVAPLLRF RSILFDEEPI TLASLSRPSL RLRVASLQKV LVRVYNAVSS GGSGGGANLP ASSKPVPHVA TLLTWTLQAH GLLCSGGVPS PDWTPTPDIG AGNSSSGENA AAVAEGGAGG TVAVAAATAM LDKYPKVSLE MLIQHLGYLV NTNAAAAGAG SQETHFCPPR SRSFGLTAVE ALLMTATQPD QIHQILPLYA RGHSLSDAAA KDITGTRNVH EALRKTQQAV GGAVAQAETE LTTLAANPPR VRDVNAVAAG LHPALAHLPS HLFVRAMGSQ GPPPGWSYYG IHLGIKAEAM NVRYCLNAGD TATPRVIDAG VLSQPDTLFV WAARGSEGEG TWTAVPCAAA GSSSAAAAAS TPSGEADAAA AVSTLRVLTW NSQFDRHSKQ ETPLGRAGID WCTATRYIAA SKVLAAAEAD VICLQEVEPA WVSFLCAQSW VREAYALSAV DISPALHPHG VLLLVRRGVG VVKRVRHVNL PATAGYVAMM PELMLQVGRE VAPVVVGGLH LVAPYTQRNE NDRVAQLAKL ESHLTQQMER GWEPATAAAA AADAVDADAA ARGEKPASGS AASTEEEKED VSYLLIGDFN DYPTRPYRPP AALAYKDAWE LLNPNCHRST ATAAERGYTI DGAANPYTAK IIEPKFFGRP DRILFRSKQL QPIDAELVGT ATVMEVLPKP AGGGPLMKVN NPAEAVPDYL YPSDHFGVLV EFQLV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtacg cgagaagtcg cctctttctg tacgttagcg tccggggcag cggcagcggc cccgcatcgt ccacagcagc gggaggcagc agcagcagca gcagcagcgg caggagagac tacatgacct ccgagggcat tgccgacgcc tttcatgcat cccaccaccg cgctgcgtgg accttcgacc acgtccttgt cgacatcccc tccatcatca actcggtcgc cttcatggct cgtgatcttg ttgggcacga gcgggacaag gaaacggtgc gcaacgcaca ccggcagctg cagacgatta ttctgcgccg tctgcaaccc cgtaagagtc tcgctcttct cttcgacggc agtgagccgc tgtggatgct ggagaagaca cgtctgtttc ctggtcgacg atacgactca aagttttacc ggagctgtgc ctcaccgatg ccgcacctgc tggaggagaa gttgcggagc actgcgatgg agttgcggac gccgccgtgg gagacagtca tcagcggtcc cgccacgccg gggctgtcgg agggaaagat gtcagcctac ctgctggacc tggccacccg catgctgcgt ccacccgcgc agcccaccgg cctctgccct gctgtgacgg cgggtgatac ggtgtgtgtg gtcggggccc cggagctggc atggatgggg cttggcgtga cgcccttcca gaacatcacg acggtgaccc tgcagcaggg cgagctgacg agctgctcgg tgcaggagac gatggcgtgg ctgcggctgg ggcacttgac gggccctaca gacggcgcag cgacgacgac tgctgtcgct ggtgcggagg gagagagcag cgtggacctt gcccagcaac agcagcagca gcagcaacag cagcggcggc tctctgctgc tcgcacagat gttgtctttc tctatctctt aacccacggt cacagcgcaa cagggctgcc gcaggtgctg tcgacgccgt ttgccgatgt gctggacgcc tacatcgcgc tggaggcgga gaacgctcaa cggacactcg atgcatcacc accgctgtcg agcggtgaag aagacggcgt ggcgccgctg ctccgatttc gcagcatcct cttcgatgaa gagcccatca ccttggcaag tctcagccgc ccatcgctgc gtctgcgcgt ggcatcgttg cagaaagtac tggtgcgggt gtacaacgcc gtttcaagcg gcggcagcgg tggcggtgcc aacttgccag cgagctcgaa gcctgtgcca cacgtggcga ccttgttgac ctggacgctg caggcgcacg ggctgctgtg ctccggtggt gtgccctcgc cggattggac ccccacgcca gacatcggtg cgggcaacag cagcagcggc gagaatgcgg cggcggtggc agagggcggt gcagggggga cggtcgctgt tgccgctgcg acggcgatgc tggataaata cccaaaggtg tctcttgaga tgctcattca gcacctcggc tacctcgtca acaccaacgc tgccgctgct ggagctggca gccaagagac ccacttctgc cccccacgct ctcgaagctt cggcctcaca gctgtggagg cgctactcat gacggcgacc cagcccgacc agatccatca gattttgccc ctctacgcac ggggtcactc tctctctgac gccgctgcca aggacatcac cggcacccgc aacgtgcacg aggcgctgcg caagacacag caggccgtcg gcggcgcggt ggcgcaggcg gagacggagc tgacgacgct ggcggcgaac ccgccccgcg tgcgggatgt caacgccgtc gctgccggcc tccaccccgc cctcgcccac ctcccgtctc acctcttcgt gcgcgcgatg ggcagccagg gcccgccgcc gggctggagc tactacggca tccatctcgg catcaaggcc gaggcgatga acgtgcgcta ctgcctgaac gccggcgaca cggcgacgcc gcgggtgatc gatgccgggg tgctgagcca gccggacacg ctctttgtgt gggctgcgcg gggcagcgag ggagagggga cgtggacagc ggtgccctgt gctgctgctg gctcctcttc agcagctgca gcagcatcaa cgccgtcggg agaagccgat gcagcggcgg cggtgtcaac gctgcgggtt ctgacgtgga atagtcagtt tgatcgtcac agcaagcagg agacaccgct tggtcgggct ggcattgact ggtgcaccgc cacgcgctac attgctgctt cgaaggtgtt ggcggcagca gaggccgacg tcatttgcct gcaggaagtc gaacccgcgt gggtgagctt cctctgtgcc cagtcgtggg tgcgggaggc gtacgcgctc agtgctgtcg acatcagccc cgctctgcac ccgcacggcg ttcttcttct cgtacgccgc ggtgtcggtg tggtgaagcg cgtgcgccac gtgaacctcc cggctactgc ggggtacgtt gcgatgatgc cggagctgat gctgcaggtg ggtcgcgagg tagctccggt ggttgtcggt ggccttcacc ttgtggcgcc gtacacgcag agaaacgaga acgatcgtgt ggcgcagctc gcgaagctgg agagccacct gacgcaacag atggagcgtg ggtgggaacc tgcgacagcc gcagcggcag cagcagatgc agtagatgca gatgcagcag cgagggggga aaaacctgca agcgggagtg cggcgagcac cgaagaagaa aaagaagacg tctcctacct gcttattggc gacttcaacg actacccgac gaggccatac cgtccacccg cagcgctggc gtacaaggac gcgtgggagc tgctgaaccc caactgccac cgcagcaccg ccacggctgc tgagcgtggc tacacgatcg acggcgccgc aaacccgtac acggcgaaga tcattgagcc gaagttcttt ggccggcccg atcgcattct gttccgctcg aagcagctcc aacccatcga tgcggagctg gttgggacgg cgacggtgat ggaggtgctg cccaagccag cgggtggagg gccgctgatg aaggtgaaca acccggccga ggcggtgcca gactatctct acccatccga ccacttcggt gtactggtcg agttccagtt ggtgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for CrfaA.19512.a.B2.GE40827
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHDY MTSEGIADAF HASHHRAAWT FDHVLVDIPS IINSVAFMAR DLVGHERDKE TVRNAHRQLQ TIILRRLQPR KSLALLFDGS EPLWMLEKTR LFPGRRYDSK FYRSCASPMP HLLEEKLRST AMELRTPPWE TVISGPATPG LSEGKMSAYL LDLATRMLRP PAQPTGLCPA VTAGDTVCVV GAPELAWMGL GVTPFQNITT VTLQQGELTS CSVQETMAWL RLGHLTGPTD GAATTTAVAG AEGESSVDLA QQQQQQQQQQ RRLSAARTDV VFLYLLTHGH SATGLPQVLS TPFADVLDAY IALEAENAQR TLDASPPLSS GEEDGVAPLL RFRSILFDEE PITLASLSRP SLRLRVASLQ KVLVRVYNAV SSGGSGGGAN LPASSKPVPH VATLLTWTLQ AHGLLCSGGV PSPDWTPTPD IGAGNSSSGE NAAAVAEGGA GGTVAVAAAT AMLDKYPKVS LEMLIQHLGY LVNTNAAAAG AGSQETHFCP PRSRSFGLTA VEALLMTATQ PDQIHQILPL YARGHSLSDA AAKDITGTRN VHEALRKTQQ AVGGAVAQAE TELTTLAANP PRVRDVNAVA AGLHPALAHL PSHLFVRAMG SQGPPPGWSY YGIHLGIKAE AMNVRYCLNA GDTATPRVID A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgactaca tgacctccga gggcattgcc gacgcctttc atgcatccca ccaccgcgct gcgtggacct tcgaccacgt ccttgtcgac atcccctcca tcatcaactc ggtcgccttc atggctcgtg atcttgttgg gcacgagcgg gacaaggaaa cggtgcgcaa cgcacaccgg cagctgcaga cgattattct gcgccgtctg caaccccgta agagtctcgc tcttctcttc gacggcagtg agccgctgtg gatgctggag aagacacgtc tgtttcctgg tcgacgatac gactcaaagt tttaccggag ctgtgcctca ccgatgccgc acctgctgga ggagaagttg cggagcactg cgatggagtt gcggacgccg ccgtgggaga cagtcatcag cggtcccgcc acgccggggc tgtcggaggg aaagatgtca gcctacctgc tggacctggc cacccgcatg ctgcgtccac ccgcgcagcc caccggcctc tgccctgctg tgacggcggg tgatacggtg tgtgtggtcg gggccccgga gctggcatgg atggggcttg gcgtgacgcc cttccagaac atcacgacgg tgaccctgca gcagggcgag ctgacgagct gctcggtgca ggagacgatg gcgtggctgc ggctggggca cttgacgggc cctacagacg gcgcagcgac gacgactgct gtcgctggtg cggagggaga gagcagcgtg gaccttgccc agcaacagca gcagcagcag caacagcagc ggcggctctc tgctgctcgc acagatgttg tctttctcta tctcttaacc cacggtcaca gcgcaacagg gctgccgcag gtgctgtcga cgccgtttgc cgatgtgctg gacgcctaca tcgcgctgga ggcggagaac gctcaacgga cactcgatgc atcaccaccg ctgtcgagcg gtgaagaaga cggcgtggcg ccgctgctcc gatttcgcag catcctcttc gatgaagagc ccatcacctt ggcaagtctc agccgcccat cgctgcgtct gcgcgtggca tcgttgcaga aagtactggt gcgggtgtac aacgccgttt caagcggcgg cagcggtggc ggtgccaact tgccagcgag ctcgaagcct gtgccacacg tggcgacctt gttgacctgg acgctgcagg cgcacgggct gctgtgctcc ggtggtgtgc cctcgccgga ttggaccccc acgccagaca tcggtgcggg caacagcagc agcggcgaga atgcggcggc ggtggcagag ggcggtgcag gggggacggt cgctgttgcc gctgcgacgg cgatgctgga taaataccca aaggtgtctc ttgagatgct cattcagcac ctcggctacc tcgtcaacac caacgctgcc gctgctggag ctggcagcca agagacccac ttctgccccc cacgctctcg aagcttcggc ctcacagctg tggaggcgct actcatgacg gcgacccagc ccgaccagat ccatcagatt ttgcccctct acgcacgggg tcactctctc tctgacgccg ctgccaagga catcaccggc acccgcaacg tgcacgaggc gctgcgcaag acacagcagg ccgtcggcgg cgcggtggcg caggcggaga cggagctgac gacgctggcg gcgaacccgc cccgcgtgcg ggatgtcaac gccgtcgctg ccggcctcca ccccgccctc gcccacctcc cgtctcacct cttcgtgcgc gcgatgggca gccagggccc gccgccgggc tggagctact acggcatcca tctcggcatc aaggccgagg cgatgaacgt gcgctactgc ctgaacgccg gcgacacggc gacgccgcgg gtgatcgatg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for CrfaA.19512.a.B3.GE40828
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV STLRVLTWNS QFDRHSKQET PLGRAGIDWC TATRYIAASK VLAAAEADVI CLQEVEPAWV SFLCAQSWVR EAYALSAVDI SPALHPHGVL LLVRRGVGVV KRVRHVNLPA TAGYVAMMPE LMLQVGREVA PVVVGGLHLV APYTQRNEND RVAQLAKLES HLTQQMERGW EPATAAAAAA DAVDADAAAR GEKPASGSAA STEEEKEDVS YLLIGDFNDY PTRPYRPPAA LAYKDAWELL NPNCHRSTAT AAERGYTIDG AANPYTAKII EPKFFGRPDR ILFRSKQLQP IDAELVGTAT VMEVLPKPAG GGPLMKVNNP AEAVPDYLYP SDHFG
Validated NT Sequence
acaccgaagt ggtcggatgg gtagagatag tctggcaccg cctcggccgg gttgttcacc ttcatcagcg gccctccacc cgctggcttg ggcagcacct ccatcaccgt cgccgtccca accagctccg catcgatggg ttggagctgc ttcgagcgga acagaatgcg atcgggccgg ccaaagaact tcggctcaat gatcttcgcc gtgtacgggt ttgcggcgcc gtcgatcgtg tagccacgct cagcagccgt ggcggtgctg cggtggcagt tggggttcag cagctcccac gcgtccttgt acgccagcgc tgcgggtgga cggtatggcc tcgtcgggta gtcgttgaag tcgccaataa gcaggtagga gacgtcttct ttttcttctt cggtgctcgc cgcactcccg cttgcaggtt tttcccccct cgctgctgca tctgcatcta ctgcatctgc tgctgccgct gcggctgtcg caggttccca cccacgctcc atctgttgcg tcaggtggct ctccagcttc gcgagctgcg ccacacgatc gttctcgttt ctctgcgtgt acggcgccac aaggtgaagg ccaccgacaa ccaccggagc tacctcgcga cccacctgca gcatcagctc cggcatcatc gcaacgtacc ccgcagtagc cgggaggttc acgtggcgca cgcgcttcac cacaccgaca ccgcggcgta cgagaagaag aacgccgtgc gggtgcagag cggggctgat gtcgacagca ctgagcgcgt acgcctcccg cacccacgac tgggcacaga ggaagctcac ccacgcgggt tcgacttcct gcaggcaaat gacgtcggcc tctgctgccg ccaacacctt cgaagcagca atgtagcgcg tggcggtgca ccagtcaatg ccagcccgac caagcggtgt ctcctgcttg ctgtgacgat caaactgact attccacgtc agaacccgca gcgttgacac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHVS TLRVLTWNSQ FDRHSKQETP LGRAGIDWCT ATRYIAASKV LAAAEADVIC LQEVEPAWVS FLCAQSWVRE AYALSAVDIS PALHPHGVLL LVRRGVGVVK RVRHVNLPAT AGYVAMMPEL MLQVGREVAP VVVGGLHLVA PYTQRNENDR VAQLAKLESH LTQQMERGWE PATAAAAAAD AVDADAAARG EKPASGSAAS TEEEKEDVSY LLIGDFNDYP TRPYRPPAAL AYKDAWELLN PNCHRSTATA AERGYTIDGA ANPYTAKIIE PKFFGRPDRI LFRSKQLQPI DAELVGTATV MEVLPKPAGG GPLMKVNNPA EAVPDYLYPS DHFGVLVEFQ LV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcaa cgctgcgggt tctgacgtgg aatagtcagt ttgatcgtca cagcaagcag gagacaccgc ttggtcgggc tggcattgac tggtgcaccg ccacgcgcta cattgctgct tcgaaggtgt tggcggcagc agaggccgac gtcatttgcc tgcaggaagt cgaacccgcg tgggtgagct tcctctgtgc ccagtcgtgg gtgcgggagg cgtacgcgct cagtgctgtc gacatcagcc ccgctctgca cccgcacggc gttcttcttc tcgtacgccg cggtgtcggt gtggtgaagc gcgtgcgcca cgtgaacctc ccggctactg cggggtacgt tgcgatgatg ccggagctga tgctgcaggt gggtcgcgag gtagctccgg tggttgtcgg tggccttcac cttgtggcgc cgtacacgca gagaaacgag aacgatcgtg tggcgcagct cgcgaagctg gagagccacc tgacgcaaca gatggagcgt gggtgggaac ctgcgacagc cgcagcggca gcagcagatg cagtagatgc agatgcagca gcgagggggg aaaaacctgc aagcgggagt gcggcgagca ccgaagaaga aaaagaagac gtctcctacc tgcttattgg cgacttcaac gactacccga cgaggccata ccgtccaccc gcagcgctgg cgtacaagga cgcgtgggag ctgctgaacc ccaactgcca ccgcagcacc gccacggctg ctgagcgtgg ctacacgatc gacggcgccg caaacccgta cacggcgaag atcattgagc cgaagttctt tggccggccc gatcgcattc tgttccgctc gaagcagctc caacccatcg atgcggagct ggttgggacg gcgacggtga tggaggtgct gcccaagcca gcgggtggag ggccgctgat gaaggtgaac aacccggccg aggcggtgcc agactatctc tacccatccg accacttcgg tgtactggtc gagttccagt tggtgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat