CrfaA.19511.a

KREN3

CENTER ID: CrfaA.19511.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.19511.a.B1.GE41379 full length 1 590
CrfaA.19511.a.B2.GE41380 RNase III-like catalytic-like domain 183 442
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CFAC1_250016900

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPPHLFESP AVRDIIYDFL PQNVILPSAA AWAEHTVNSL DQPRDKAFSF LDSVTSTCCL CGPFAHGGSR LHLAARNHMV RRAIVGRALG YLYTFCSQAT RTVDEQHRSP RHQRPSGGGS NGAAAGPGIT REMAAASSYH TSYARSPLFD EPGVPSEEEL FHNRDALHKL DLVELLLRRW WRMLNQVPVE KGGYAFDRVV SLSSDVSETR GSRLRYLLFF LASRGVLRNS VQMSNIATSN TSDLQSCKPH RGPAFERVEM AGDLVFKTFV EDRMHAMFPA DEGGVTLYMD EVRRVVDCNH GLLTVYDYLN LDDLRSQALA NNKSKADIVE AIAGELQAQL WTMEVYYNTD AYPLPAERAN ARYLHAVVQH TLQELGHVAL MWHIENTLRG AKAIIKGCLD KAFHLDENAA GQSYTDAAAV STEFEKKALA KGSAQLPLML PWRRPRGGTR SPKNLLALLA DHRAERPRAC FPDAVAKLAC TPHGMPAEMR NRFAVHPPYA PLSYQQAQSE AVQSVLCAAP AHEFASGESP QSRGGCLFDV FMPDLQPQPH PRALEVPSAE AMRELHRVNC VVQRRTAPQL RTVGNASRCT
NT Sequence
ATGCCGCCGC CGCACCTCTT CGAGTCCCCC GCGGTGCGAG ACATCATCTA TGACTTTCTG CCCCAAAACG TGATCTTACC CAGCGCCGCT GCGTGGGCGG AGCACACGGT CAACAGTCTC GACCAACCCC GCGACAAGGC CTTTTCTTTT CTCGACTCGG TCACGTCCAC CTGCTGCCTA TGCGGCCCTT TTGCGCACGG CGGGTCGCGG CTGCACCTGG CAGCTCGAAA CCACATGGTG CGCCGCGCAA TCGTGGGGCG TGCCTTGGGC TACCTTTACA CGTTCTGCAG TCAGGCGACG CGGACGGTGG ACGAGCAGCA CCGCTCTCCG CGGCACCAGC GACCGAGTGG CGGCGGCTCC AACGGCGCCG CTGCGGGGCC GGGAATCACG CGCGAGATGG CGGCGGCCTC GAGCTACCAC ACCTCCTACG CCCGCTCTCC TCTCTTTGAC GAGCCAGGTG TCCCGAGTGA GGAGGAACTC TTTCACAACC GTGACGCACT GCACAAGCTG GACCTTGTCG AGCTGCTGCT GCGCCGGTGG TGGCGCATGT TGAACCAGGT CCCCGTCGAA AAGGGTGGTT ACGCGTTTGA CCGCGTCGTG TCCCTGTCGT CCGACGTGTC AGAGACGCGC GGGAGTCGGC TGCGCTACCT CCTCTTCTTC CTCGCCTCGC GTGGTGTGCT GCGCAACTCG GTGCAGATGT CTAACATCGC CACGAGCAAC ACCTCAGATT TGCAGAGCTG CAAGCCGCAC CGTGGGCCGG CTTTCGAGCG CGTTGAGATG GCGGGCGACT TGGTGTTCAA AACCTTCGTA GAAGACCGCA TGCACGCCAT GTTCCCCGCT GACGAGGGCG GCGTGACGCT GTACATGGAC GAGGTGAGAC GCGTGGTCGA CTGCAACCAC GGCCTGCTCA CCGTGTATGA CTACCTAAAC CTTGACGACT TGCGCAGCCA GGCGCTGGCA AACAACAAGT CCAAAGCGGA CATCGTTGAG GCCATCGCCG GAGAGTTGCA GGCGCAGCTC TGGACGATGG AGGTGTACTA CAACACAGAC GCCTACCCGT TGCCGGCGGA GCGGGCCAAC GCGCGCTACC TGCACGCTGT GGTGCAGCAC ACCCTTCAGG AGCTGGGCCA CGTGGCCCTC ATGTGGCACA TCGAAAACAC TCTGCGCGGC GCCAAAGCCA TCATCAAAGG CTGCCTGGAT AAGGCGTTCC ATCTGGACGA GAACGCCGCC GGGCAGTCGT ACACCGACGC TGCGGCGGTG AGTACCGAGT TCGAGAAGAA GGCGCTGGCA AAAGGGTCGG CGCAGCTGCC CTTGATGCTG CCGTGGCGCC GCCCCCGTGG TGGCACACGC TCGCCCAAGA ACCTCCTTGC CTTGTTGGCG GACCACCGAG CGGAGCGGCC GCGAGCTTGC TTTCCCGATG CCGTTGCGAA ACTCGCTTGC ACCCCACACG GGATGCCAGC GGAGATGCGT AACCGTTTTG CGGTGCATCC GCCCTACGCG CCGCTGTCGT ACCAGCAAGC ACAGAGCGAG GCGGTGCAGT CTGTCCTGTG CGCCGCTCCT GCACACGAGT TCGCGTCAGG GGAGTCGCCG CAAAGCCGAG GCGGGTGTCT CTTTGACGTT TTCATGCCAG ATCTGCAGCC GCAGCCCCAC CCGCGCGCTC TGGAGGTGCC ATCCGCTGAG GCAATGCGCG AGCTGCACCG CGTGAACTGC GTCGTGCAAC GAAGAACGGC ACCACAGCTC CGCACCGTGG GAAACGCTTC CCGATGCACG TGA
Details for CrfaA.19511.a.B1.GE41379
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 66kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP PPHLFESPAV RDIIYDFLPQ NVILPSAAAW AEHTVNSLDQ PRDKAFSFLD SVTSTCCLCG PFAHGGSRLH LAARNHMVRR AIVGRALGYL YTFCSQATRT VDEQHRSPRH QRPSGGGSNG AAAGPGITRE MAAASSYHTS YARSPLFDEP GVPSEEELFH NRDALHKLDL VELLLRRWWR MLNQVPVEKG GYAFDRVVSL SSDVSETRGS RLRYLLFFLA SRGVLRNSVQ MSNIATSNTS DLQSCKPHRG PAFERVEMAG DLVFKTFVED RMHAMFPADE GGVTLYMDEV RRVVDCNHGL LTVYDYLNLD DLRSQALANN KSKADIVEAI AGELQAQLWT MEVYYNTDAY PLPAERANAR YLHAVVQHTL QELGHVALMW HIENTLRGAK AIIKGCLDKA FHLDENAAGQ SYTDAAAVST EFEKKALAKG SAQLPLMLPW RRPRGGTRSP KNLLALLADH RAERPRACFP DAVAKLACTP HGMPAEMRNR FAVHPPYAPL SYQQAQSEAV QSVLCAAPAH EFASGESPQS RGGCLFDVFM PDLQPQPHPR ALEVPSAEAM RELHRVNCVV QRRTAPQLRT VGNASRCT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc cgccgcacct cttcgagtcc cccgcggtgc gagacatcat ctatgacttt ctgccccaaa acgtgatctt acccagcgcc gctgcgtggg cggagcacac ggtcaacagt ctcgaccaac cccgcgacaa ggccttttct tttctcgact cggtcacgtc cacctgctgc ctatgcggcc cttttgcgca cggcgggtcg cggctgcacc tggcagctcg aaaccacatg gtgcgccgcg caatcgtggg gcgtgccttg ggctaccttt acacgttctg cagtcaggcg acgcggacgg tggacgagca gcaccgctct ccgcggcacc agcgaccgag tggcggcggc tccaacggcg ccgctgcggg gccgggaatc acgcgcgaga tggcggcggc ctcgagctac cacacctcct acgcccgctc tcctctcttt gacgagccag gtgtcccgag tgaggaggaa ctctttcaca accgtgacgc actgcacaag ctggaccttg tcgagctgct gctgcgccgg tggtggcgca tgttgaacca ggtccccgtc gaaaagggtg gttacgcgtt tgaccgcgtc gtgtccctgt cgtccgacgt gtcagagacg cgcgggagtc ggctgcgcta cctcctcttc ttcctcgcct cgcgtggtgt gctgcgcaac tcggtgcaga tgtctaacat cgccacgagc aacacctcag atttgcagag ctgcaagccg caccgtgggc cggctttcga gcgcgttgag atggcgggcg acttggtgtt caaaaccttc gtagaagacc gcatgcacgc catgttcccc gctgacgagg gcggcgtgac gctgtacatg gacgaggtga gacgcgtggt cgactgcaac cacggcctgc tcaccgtgta tgactaccta aaccttgacg acttgcgcag ccaggcgctg gcaaacaaca agtccaaagc ggacatcgtt gaggccatcg ccggagagtt gcaggcgcag ctctggacga tggaggtgta ctacaacaca gacgcctacc cgttgccggc ggagcgggcc aacgcgcgct acctgcacgc tgtggtgcag cacacccttc aggagctggg ccacgtggcc ctcatgtggc acatcgaaaa cactctgcgc ggcgccaaag ccatcatcaa aggctgcctg gataaggcgt tccatctgga cgagaacgcc gccgggcagt cgtacaccga cgctgcggcg gtgagtaccg agttcgagaa gaaggcgctg gcaaaagggt cggcgcagct gcccttgatg ctgccgtggc gccgcccccg tggtggcaca cgctcgccca agaacctcct tgccttgttg gcggaccacc gagcggagcg gccgcgagct tgctttcccg atgccgttgc gaaactcgct tgcaccccac acgggatgcc agcggagatg cgtaaccgtt ttgcggtgca tccgccctac gcgccgctgt cgtaccagca agcacagagc gaggcggtgc agtctgtcct gtgcgccgct cctgcacacg agttcgcgtc aggggagtcg ccgcaaagcc gaggcgggtg tctctttgac gttttcatgc cagatctgca gccgcagccc cacccgcgcg ctctggaggt gccatccgct gaggcaatgc gcgagctgca ccgcgtgaac tgcgtcgtgc aacgaagaac ggcaccacag ctccgcaccg tgggaaacgc ttcccgatgc acgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for CrfaA.19511.a.B2.GE41380
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML NQVPVEKGGY AFDRVVSLSS DVSETRGSRL RYLLFFLASR GVLRNSVQMS NIATSNTSDL QSCKPHRGPA FERVEMAGDL VFKTFVEDRM HAMFPADEGG VTLYMDEVRR VVDCNHGLLT VYDYLNLDDL RSQALANNKS KADIVEAIAG ELQAQLWTME VYYNTDAYPL PAERANARYL HAVVQHTLQE LGHVALMWHI ENTLRGAKAI IKGCLDKAFH LDENAAGQSY TDAAAVSTEF EKKALAKGSA XXXXMLPW
Validated NT Sequence
atggctcacc accaccacca ccatatgttg aaccaggtcc ccgtcgaaaa gggtggttac gcgtttgacc gcgtcgtgtc cctgtcgtcc gacgtgtcag agacgcgcgg gagtcggctg cgctacctcc tcttcttcct cgcctcgcgt ggtgtgctgc gcaactcggt gcagatgtct aacatcgcca cgagcaacac ctcagatttg cagagctgca agccgcaccg tgggccggct ttcgagcgcg ttgagatggc gggcgacttg gtgttcaaaa ccttcgtaga agaccgcatg cacgccatgt tccccgctga cgagggcggc gtgacgctgt acatggacga ggtgagacgc gtggtcgact gcaaccacgg cctgctcacc gtgtatgact acctaaacct tgatgacttg cgcagccagg cgctggcaaa caacaagtcc aaagcggaca tcgttgaggc catcgccgga gagttgcagg cgcaactctg gacgatggag gtgtattaca acacagacgc ctacccgttg ccggcggagc gggccaacgc gcgctacctg cacgctgtgg tgcagcacac ccttcaggag ctgggccacg tggccctcat gtggcacatc gaaaacactc tgcgcggcgc caaagccatc atcaaaggct gcctggataa ggcgttccat ctggacgaga acgccgccgg gcagtcgtac accgacgctg cggcggtgag taccgagttc gagaagaagg cgctggcaaa agggtcggcg canntgnnnn tgatgctgcc gtggtaagtg agtaagat
Expected Protein Sequence
MAHHHHHHML NQVPVEKGGY AFDRVVSLSS DVSETRGSRL RYLLFFLASR GVLRNSVQMS NIATSNTSDL QSCKPHRGPA FERVEMAGDL VFKTFVEDRM HAMFPADEGG VTLYMDEVRR VVDCNHGLLT VYDYLNLDDL RSQALANNKS KADIVEAIAG ELQAQLWTME VYYNTDAYPL PAERANARYL HAVVQHTLQE LGHVALMWHI ENTLRGAKAI IKGCLDKAFH LDENAAGQSY TDAAAVSTEF EKKALAKGSA QLPLMLPW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga accaggtccc cgtcgaaaag ggtggttacg cgtttgaccg cgtcgtgtcc ctgtcgtccg acgtgtcaga gacgcgcggg agtcggctgc gctacctcct cttcttcctc gcctcgcgtg gtgtgctgcg caactcggtg cagatgtcta acatcgccac gagcaacacc tcagatttgc agagctgcaa gccgcaccgt gggccggctt tcgagcgcgt tgagatggcg ggcgacttgg tgttcaaaac cttcgtagaa gaccgcatgc acgccatgtt ccccgctgac gagggcggcg tgacgctgta catggacgag gtgagacgcg tggtcgactg caaccacggc ctgctcaccg tgtatgacta cctaaacctt gacgacttgc gcagccaggc gctggcaaac aacaagtcca aagcggacat cgttgaggcc atcgccggag agttgcaggc gcagctctgg acgatggagg tgtactacaa cacagacgcc tacccgttgc cggcggagcg ggccaacgcg cgctacctgc acgctgtggt gcagcacacc cttcaggagc tgggccacgt ggccctcatg tggcacatcg aaaacactct gcgcggcgcc aaagccatca tcaaaggctg cctggataag gcgttccatc tggacgagaa cgccgccggg cagtcgtaca ccgacgctgc ggcggtgagt accgagttcg agaagaaggc gctggcaaaa gggtcggcgc agctgccctt gatgctgccg tggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat