SSGCID: Target Page

CrfaA.19507.a

KREPB5

CENTER ID:	CrfaA.19507.a
ORGANISM:	Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrfaA.19507.a.B1.GE41377	full length		1	433

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CFAC1_230049400

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRVAHAIRK VGLHNAAAAT QRQQIGAVLV ARASFAQSNV SLSTSPRPPR KAGARDPLPT PSLPPGLRAV LFDMLAQRLC SPTNPLPARG VYDVLANRSR IVAEFHYAKT PKRLSRQRVA LAFLRRYNVL LHSLLFFSNN SQTWNITPEF HRLALLGESV LTNEVRGRLL KLFPDMAYTT LVQILQHLLG EKGLCAAFDR FHLTEVVGAK PSIARQLRSI TAEQKCHMLC AVIGEMYWFV ARTRPTDRTH NNALFPPSDV LILNVLCSHL LECIPAELIY HIVEPVVAEM KGVWVNEPMS LPAQLRLTPR TIGALSLNTV PLPRPAPTAA TNAGDAQTTA SITHAHKSAQ SVRRTLTTTP DARFVKSFMR PQCNHRNFDH PRYQVLESDT VREVCALSPT KEIAANRGTE GALDSEAARV ARVMELAKLA LHQ

NT Sequence

ATGAGGCGAG TGGCGCACGC GATACGAAAG GTAGGTTTGC ATAACGCTGC CGCGGCCACG CAGCGCCAGC AGATAGGAGC TGTTCTTGTC GCCCGTGCGT CTTTTGCGCA GAGCAACGTG TCTCTCAGCA CTAGTCCACG GCCGCCGCGC AAAGCCGGAG CGCGTGACCC GCTGCCCACC CCGTCCCTGC CGCCGGGCTT GCGGGCGGTG CTCTTCGACA TGCTTGCGCA GCGGCTGTGC AGCCCGACAA ACCCGCTGCC CGCACGCGGC GTGTACGACG TACTGGCCAA CCGCTCCCGC ATTGTTGCGG AGTTCCACTA CGCCAAGACA CCCAAACGCC TCTCTCGCCA ACGCGTCGCG CTTGCGTTCT TGCGGCGCTA CAACGTGCTG CTGCACAGCC TCCTCTTCTT CTCAAATAAC TCGCAGACGT GGAACATCAC CCCAGAGTTT CACCGCCTCG CGCTCTTGGG CGAGTCCGTC TTGACGAACG AGGTGCGTGG CCGCTTGCTG AAGCTCTTCC CTGATATGGC GTACACGACG CTGGTACAGA TTCTGCAGCA CCTTCTCGGC GAGAAGGGCC TTTGTGCCGC CTTCGACCGC TTCCACCTGA CGGAAGTTGT CGGCGCCAAG CCCTCGATTG CACGGCAGCT GCGCAGCATC ACCGCTGAGC AAAAGTGTCA CATGCTCTGC GCCGTGATTG GGGAGATGTA CTGGTTTGTG GCCCGCACGC GGCCGACGGA CCGCACGCAC AACAACGCTC TCTTTCCGCC ATCCGACGTG CTCATTTTGA ACGTGCTGTG CTCCCACCTG CTCGAGTGCA TTCCGGCCGA GTTGATTTAC CATATCGTCG AGCCCGTCGT TGCCGAGATG AAGGGTGTGT GGGTGAACGA GCCCATGTCG CTGCCGGCGC AGCTGCGGCT CACCCCACGC ACCATCGGTG CCCTCTCCCT CAACACCGTT CCCCTCCCAC GCCCGGCACC CACCGCCGCA ACAAACGCCG GTGACGCGCA GACGACGGCG TCGATTACGC ACGCGCACAA GAGCGCGCAG TCGGTGCGGC GAACCCTCAC AACAACTCCC GATGCGCGCT TCGTGAAGTC CTTTATGCGG CCGCAGTGCA ACCACCGCAA CTTCGACCAC CCACGCTATC AGGTGCTCGA GTCTGACACG GTGAGGGAGG TGTGTGCGCT GTCTCCTACA AAGGAGATTG CTGCCAATCG CGGCACGGAA GGTGCGCTGG ACAGCGAGGC GGCTCGTGTT GCTCGCGTGA TGGAGCTGGC CAAACTCGCT TTGCATCAGT GA

Details for CrfaA.19507.a.B1.GE41377

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR RVAHAIRKVG LHNAAAATQR QQIGAVLVAR ASFAQSNVSL STSPRPPRKA GARDPLPTPS LPPGLRAVLF DMLAQRLCSP TNPLPARGVY DVLANRSRIV AEFHYAKTPK RLSRQRVALA FLRRYNVLLH SLLFFSNNSQ TWNITPEFHR LALLGESVLT NEVRGRLLKL FPDMAYTTLV QILQHLLGEK GLCAAFDRFH LTEVVGAKPS IARQLRSITA EQKCHMLCAV IGEMYWFVAR TRPTDRTHNN ALFPPSDVLI LNVLCSHLLE CIPAELIYHI VEPVVAEMKG VWVNEPMSLP AQLRLTPRTI GALSLNTVPL PRPAPTAATN AGDAQTTASI THAHKSAQSV RRTLTTTPDA RFVKSFMRPQ CNHRNFDHPR YQVLESDTVR EVCALSSTKE IAANRGTEGA LDSEAARVAR VME

Validated NT Sequence

atggctcacc accaccacca ccatatgagg cgagtggcgc acgcgatacg aaaggtaggt ttgcataacg ctgccgcggc cacgcagcgc cagcagatag gagctgttct tgtcgcccgt gcgtcttttg cgcagagcaa cgtgtctctc agcactagtc cacggccgcc gcgcaaagcc ggagcgcgtg acccgctgcc caccccgtcc ctgccgccgg gcttgcgggc ggtgctcttc gacatgcttg cgcagcggct gtgcagcccg acaaacccgc tgcccgcacg cggcgtgtac gacgtactgg ccaaccgctc ccgcattgtt gcggagttcc actacgccaa gacacccaaa cgcctctctc gccaacgcgt cgcgcttgcg ttcttgcggc gctacaacgt gctgctgcac agcctcctct tcttctcaaa taactcgcag acgtggaaca tcaccccaga gtttcaccgc ctcgcgctct tgggcgagtc cgtcttgacg aacgaggtgc gtggccgctt gctgaagctc ttccctgata tggcgtacac gacgctggta cagattctgc agcaccttct cggcgagaag ggcctttgtg ccgccttcga ccgcttccac ctgacggaag ttgtcggcgc caagccctcg attgcacggc agctgcgcag catcaccgct gagcaaaagt gtcacatgct ctgcgccgtg attggggaga tgtactggtt tgtggcccgc acgcggccga cggaccgcac gcacaacaac gctctctttc cgccatccga cgtgctcatt ttgaacgtgc tgtgctccca cctgctcgag tgcattccgg ccgagttgat ttaccatatc gtcgagcccg tcgttgccga gatgaagggt gtgtgggtga acgagcccat gtcgctgccg gcgcagctgc ggctcacccc acgcaccatc ggtgccctct ccctcaacac cgttcccctc ccacgcccgg cacccaccgc cgcaacaaac gccggtgacg cgcagacgac ggcgtcgatt acgcacgcgc acaagagcgc gcagtcggtg cggcgaaccc tcacaacaac tcccgatgcg cgcttcgtga agtcctttat gcggccgcag tgcaaccacc gcaacttcga ccacccacgc tatcaggtgc tcgagtctga cacggtgagg gaggtgtgtg cgctgtcttc tacaaaggag attgctgcca atcgcggcac ggaaggtgcg ctggacagcg aggcggctcg tgttgctcgc gtgatggagc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaggc gagtggcgca cgcgatacga aaggtaggtt tgcataacgc tgccgcggcc acgcagcgcc agcagatagg agctgttctt gtcgcccgtg cgtcttttgc gcagagcaac gtgtctctca gcactagtcc acggccgccg cgcaaagccg gagcgcgtga cccgctgccc accccgtccc tgccgccggg cttgcgggcg gtgctcttcg acatgcttgc gcagcggctg tgcagcccga caaacccgct gcccgcacgc ggcgtgtacg acgtactggc caaccgctcc cgcattgttg cggagttcca ctacgccaag acacccaaac gcctctctcg ccaacgcgtc gcgcttgcgt tcttgcggcg ctacaacgtg ctgctgcaca gcctcctctt cttctcaaat aactcgcaga cgtggaacat caccccagag tttcaccgcc tcgcgctctt gggcgagtcc gtcttgacga acgaggtgcg tggccgcttg ctgaagctct tccctgatat ggcgtacacg acgctggtac agattctgca gcaccttctc ggcgagaagg gcctttgtgc cgccttcgac cgcttccacc tgacggaagt tgtcggcgcc aagccctcga ttgcacggca gctgcgcagc atcaccgctg agcaaaagtg tcacatgctc tgcgccgtga ttggggagat gtactggttt gtggcccgca cgcggccgac ggaccgcacg cacaacaacg ctctctttcc gccatccgac gtgctcattt tgaacgtgct gtgctcccac ctgctcgagt gcattccggc cgagttgatt taccatatcg tcgagcccgt cgttgccgag atgaagggtg tgtgggtgaa cgagcccatg tcgctgccgg cgcagctgcg gctcacccca cgcaccatcg gtgccctctc cctcaacacc gttcccctcc cacgcccggc acccaccgcc gcaacaaacg ccggtgacgc gcagacgacg gcgtcgatta cgcacgcgca caagagcgcg cagtcggtgc ggcgaaccct cacaacaact cccgatgcgc gcttcgtgaa gtcctttatg cggccgcagt gcaaccaccg caacttcgac cacccacgct atcaggtgct cgagtctgac acggtgaggg aggtgtgtgc gctgtctcct acaaaggaga ttgctgccaa tcgcggcacg gaaggtgcgc tggacagcga ggcggctcgt gttgctcgcg tgatggagct ggccaaactc gctttgcatc agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat