SSGCID: Target Page

CrfaA.19506.a

KREPB6

CENTER ID:	CrfaA.19506.a
ORGANISM:	Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrfaA.19506.a.B1.GE41064	full length		1	534

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CFAC1_200021100

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLTRCRLTFT LRTSPRHLTV DRCMTAHSLT AAVPTPSTQQ RRCSAVLGQP CLPGSGAVSR RSCATTSATP EALEGEKPSS ASASPAPTDN GAVMPVSRYS VSRSSSPISK WETVPNWCDL CCEPVNAREQ HIGKREHICL ETALQAAVAY PRRWSAPEVW EGAELFRHHY TRRPRGQPRQ LSAHMRRMLE AGLHVPAPDS PLNMFYEAFD QDEPMARREE IIVLLLHLQA CGVLHLTPAN LADAALHGSA VLHKELMPLL LRVYPDAEVR SFSAMLALSH SSWAAEALFY FAGLQALLTP ELIRNFSGKS SKALMSSLEG GDDGAESGGE GPQTDAVDGS GDGGDEAGGV SMYLRCITPR AVLGMLRWST EPEKMQVPPA ILADEKRYGH YTTLAARACR LLLSELAFLR VSEYVVRAES LMRVELTEEA ERQQSRSQRS VDEVQQRGKQ RSTLTPTQVR LIYTQRSIPH LSNEGSVGLN GGSSLSAANV LGTSTADNAI RMLEGRRALR VKRKHQSSGT ATSSKPLART VLFR

NT Sequence

ATGCTCACGC GATGCCGCCT CACGTTCACA CTGCGCACAT CACCGCGGCA TCTTACTGTC GATAGGTGCA TGACGGCCCA CTCGCTGACA GCAGCGGTCC CCACGCCCAG CACGCAGCAG CGGCGGTGCA GTGCGGTGCT TGGCCAGCCG TGTTTGCCGG GCAGCGGCGC GGTGAGCCGG CGGAGCTGCG CCACAACATC CGCTACGCCG GAGGCATTGG AGGGAGAGAA GCCATCTTCT GCTTCAGCTT CTCCCGCTCC CACCGACAAC GGTGCTGTGA TGCCCGTCTC TCGCTACAGC GTGAGCCGGA GCTCGTCGCC CATCTCCAAG TGGGAGACGG TCCCGAACTG GTGCGACCTC TGCTGCGAGC CGGTCAACGC GCGGGAGCAG CACATTGGCA AGCGAGAGCA TATCTGCCTG GAGACGGCGC TGCAGGCGGC GGTGGCCTAC CCGCGACGCT GGAGCGCGCC GGAAGTGTGG GAGGGTGCGG AGCTGTTTCG CCATCATTAC ACCCGGCGTC CGCGCGGGCA GCCACGACAG CTCTCGGCGC ACATGCGCCG CATGCTGGAG GCTGGCCTGC ACGTGCCGGC ACCAGACAGC CCGCTGAACA TGTTCTACGA GGCTTTTGAC CAAGACGAGC CAATGGCGCG GCGAGAGGAG ATCATTGTGC TGCTGCTGCA CCTCCAGGCC TGCGGGGTGC TGCACCTAAC ACCGGCGAAC CTCGCAGACG CCGCGCTGCA CGGCAGTGCG GTGCTGCACA AGGAGTTGAT GCCGCTACTG CTGCGTGTGT ACCCAGACGC GGAGGTGCGC AGCTTCTCGG CCATGTTGGC CCTCAGCCAC TCCTCCTGGG CCGCGGAGGC ACTCTTCTAC TTTGCCGGCC TGCAGGCGTT GCTCACGCCA GAACTTATTC GCAACTTCTC CGGCAAGAGC AGCAAGGCGC TGATGAGCAG CTTGGAGGGC GGTGACGACG GCGCGGAGAG TGGTGGCGAG GGACCACAGA CAGACGCGGT GGACGGCAGC GGCGACGGCG GTGATGAGGC AGGCGGCGTG TCCATGTACT TGAGGTGCAT CACACCGCGC GCTGTGCTTG GGATGCTGCG GTGGTCCACC GAGCCGGAGA AGATGCAGGT ACCACCCGCA ATTCTGGCAG ACGAGAAGCG CTACGGCCAC TACACGACGT TGGCGGCGCG GGCCTGCCGT CTGCTGCTGT CAGAGTTGGC CTTTCTGCGC GTCAGCGAGT ACGTGGTGCG CGCAGAGAGC TTGATGCGAG TGGAGCTGAC AGAGGAGGCG GAGCGGCAGC AGAGCCGGTC ACAGCGGTCG GTCGATGAGG TGCAGCAGCG CGGCAAGCAG AGGAGCACGC TGACGCCGAC GCAGGTGCGC CTGATCTACA CCCAACGCTC CATCCCTCAC CTCTCAAACG AGGGCAGCGT GGGTCTCAAC GGCGGTTCCT CTCTCTCCGC GGCAAACGTG CTCGGCACGT CAACGGCGGA CAACGCGATC CGGATGTTAG AGGGGCGGCG CGCGCTTCGG GTAAAACGAA AGCACCAGTC GAGTGGGACC GCTACCTCGT CGAAGCCCCT GGCACGCACC GTTTTGTTTC GCTGA

Details for CrfaA.19506.a.B1.GE41064

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	59kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	83
PERCENT COVERAGE:	59

Validated AA Sequence

Validated NT Sequence

aggggcttcg acgaggtagc ggtcccactc gactggtgct ttcgttttac ccgaagcgcg cgccgcccct ctaacatccg gatcgcgttg tccgccgttg acgtgccgag cacgtttgcc gcggagagag aggaaccgcc gttgagaccc acgctgccct cgtttgagag gtgagggatg gagcgttggg tgtagatcag gcgcacctgc gtcggcgtca gcgtgctcct ctgcttgccg cgctgctgca cctcatcgac cgaccgctgt gaccggctct gctgccgctc cgcctcctct gtcagctcca ctcgcatcaa gctctctgcg cgcaccacgt actcgctgac gcgcagaaag gccaactctg acagcagcag acggcaggcc cgcgccgcca acgtcgtgta gtggccgtag cgcttctcgt ctgccagaat tgcgggtggt acctgcatct tctccggctc ggtggaccac cgcagcatcc caagcacagc gcgcggtgtg atgcacctca agtacatgga cacgccgcct gcctcatcac cgccgtcgcc gctgccgtcc accgcgtctg tctgtggtcc ctcgccacca ctctccgcgc cgtcgtcacc gccctccaag ctgctcatca gcgccttgct gctcttgccg gagaagttgc gaataagttc tggcgtgagc aacgcctgca ggccggcaaa gtagaagagt gcctccgcgg cccaggagga gtggctgagg nnnnnacatg gccgagaagc tgcgcacctc cgcgtctggg tacacacgca gcagtagcgg catcaactcc ttgtgcagca ccgcactgcc gtgcagcgcg gcgtctgcga ggttcgcnnn ngnntaggtg cagcaccccg caggcctgga ggtgcagcag cagcacaatg atctcctctc gccgcgccat tggctcgtct tggtcaaaag cctcgtagaa catgttcagc gggctgtctg gtgccggcac gtgcaggcca gcctccagca tgcggcgcat gtgcgccgag agctgtcgtg gctgcccgcg cggacgccgg gtgtaatgat ggcgaaacag ctccgcaccc tcccacactt ccggcgcgct ccagcgtcgc gggtaggcca ccgccgcctg cagcgccgtc tccaggcaga tatgctctcg cttgccaatg tgctgctccc gcgcgttgac cggctcgcag cagaggtcgc accagttcgg gaccgtctcc cacttggaga tgggcgacga gctccggctc acgctgtagc gagagacggg catcacagca ccgttgtcgg tgggagcggg agaagctgaa gcagaagatg gcttctctcc ctccaatgcc tccggcgtag cggatgttgt ggcgcagctc cgccggctca ccgcgccgct gcccggcaaa cacggctggc caagcaccgc actgcaccgc cgctgctgcg tgctgggcgt ggggaccgct gctgtcagcg agtgggccgt catgcaccta tcgacagtaa gatgccgcgg tgatgtgcgc agtgtgaacg tgaggcggca tcgcgtgagc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHML TRCRLTFTLR TSPRHLTVDR CMTAHSLTAA VPTPSTQQRR CSAVLGQPCL PGSGAVSRRS CATTSATPEA LEGEKPSSAS ASPAPTDNGA VMPVSRYSVS RSSSPISKWE TVPNWCDLCC EPVNAREQHI GKREHICLET ALQAAVAYPR RWSAPEVWEG AELFRHHYTR RPRGQPRQLS AHMRRMLEAG LHVPAPDSPL NMFYEAFDQD EPMARREEII VLLLHLQACG VLHLTPANLA DAALHGSAVL HKELMPLLLR VYPDAEVRSF SAMLALSHSS WAAEALFYFA GLQALLTPEL IRNFSGKSSK ALMSSLEGGD DGAESGGEGP QTDAVDGSGD GGDEAGGVSM YLRCITPRAV LGMLRWSTEP EKMQVPPAIL ADEKRYGHYT TLAARACRLL LSELAFLRVS EYVVRAESLM RVELTEEAER QQSRSQRSVD EVQQRGKQRS TLTPTQVRLI YTQRSIPHLS NEGSVGLNGG SSLSAANVLG TSTADNAIRM LEGRRALRVK RKHQSSGTAT SSKPLARTVL FR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca cgcgatgccg cctcacgttc acactgcgca catcaccgcg gcatcttact gtcgataggt gcatgacggc ccactcgctg acagcagcgg tccccacgcc cagcacgcag cagcggcggt gcagtgcggt gcttggccag ccgtgtttgc cgggcagcgg cgcggtgagc cggcggagct gcgccacaac atccgctacg ccggaggcat tggagggaga gaagccatct tctgcttcag cttctcccgc tcccaccgac aacggtgctg tgatgcccgt ctctcgctac agcgtgagcc ggagctcgtc gcccatctcc aagtgggaga cggtcccgaa ctggtgcgac ctctgctgcg agccggtcaa cgcgcgggag cagcacattg gcaagcgaga gcatatctgc ctggagacgg cgctgcaggc ggcggtggcc tacccgcgac gctggagcgc gccggaagtg tgggagggtg cggagctgtt tcgccatcat tacacccggc gtccgcgcgg gcagccacga cagctctcgg cgcacatgcg ccgcatgctg gaggctggcc tgcacgtgcc ggcaccagac agcccgctga acatgttcta cgaggctttt gaccaagacg agccaatggc gcggcgagag gagatcattg tgctgctgct gcacctccag gcctgcgggg tgctgcacct aacaccggcg aacctcgcag acgccgcgct gcacggcagt gcggtgctgc acaaggagtt gatgccgcta ctgctgcgtg tgtacccaga cgcggaggtg cgcagcttct cggccatgtt ggccctcagc cactcctcct gggccgcgga ggcactcttc tactttgccg gcctgcaggc gttgctcacg ccagaactta ttcgcaactt ctccggcaag agcagcaagg cgctgatgag cagcttggag ggcggtgacg acggcgcgga gagtggtggc gagggaccac agacagacgc ggtggacggc agcggcgacg gcggtgatga ggcaggcggc gtgtccatgt acttgaggtg catcacaccg cgcgctgtgc ttgggatgct gcggtggtcc accgagccgg agaagatgca ggtaccaccc gcaattctgg cagacgagaa gcgctacggc cactacacga cgttggcggc gcgggcctgc cgtctgctgc tgtcagagtt ggcctttctg cgcgtcagcg agtacgtggt gcgcgcagag agcttgatgc gagtggagct gacagaggag gcggagcggc agcagagccg gtcacagcgg tcggtcgatg aggtgcagca gcgcggcaag cagaggagca cgctgacgcc gacgcaggtg cgcctgatct acacccaacg ctccatccct cacctctcaa acgagggcag cgtgggtctc aacggcggtt cctctctctc cgcggcaaac gtgctcggca cgtcaacggc ggacaacgcg atccggatgt tagaggggcg gcgcgcgctt cgggtaaaac gaaagcacca gtcgagtggg accgctacct cgtcgaagcc cctggcacgc accgttttgt ttcgctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat