SSGCID: Target Page

CrfaA.19505.a

KREX1

CENTER ID:	CrfaA.19505.a
ORGANISM:	Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrfaA.19505.a.B2.GE41375	5'-3' exonuclease		1	574
CrfaA.19505.a.B3.GE41376	endo-exonuclease-phosphatase		647	952

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CFAC1_290014700

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRGFLRVSPR CLVSYRDNRR LMDAYHLLVT KKPVEYDYVA IDVDSFISGA QRLAKNVTPE SRRNKEVARH VVTALEQLLR RVVCKQSLLF AFDGAGMAAK VHEYRHGRIT RKVESRAVRL PGMPLTRAVE ERLVRSMPLA SGLVPREVVV SGTSVEGVVE RKAAAWALDL AARDGAQHHT TRSLCFVGSN SLVHTLLAAT PYFQATHIVH GYADLRHLTL NNALAWLELD GATRSTTSSS SSGSSGGVDT RALTSGRLDA LLLMLLCTGC EVTSLPALGG AQFNLMMDAY HNKRASERGE GATAAATAAL SSPAVPREPS FQVVRELPNG VMEVDLVALL LLFLSKDAAA ELLRSAETLT TKEAEKCVHD APLSVAAHET YTNLMESYLT HLWSTLVLLC YGTTTAEAMQ VPFYLTQGDV GTCAVTVHPF ATYLRSVIAK GRRYRRVSTA CSTTGTSTDA GRGQPAYAPF WSPGQPWTAA EFTLLCQNKL VEMEPLLREY LPGIATHRVP EIARSLLSTE RTVDAARGFL QRWYATADAD AAHPSLYFSP SLCWLRNEKS RMWGVRYVDL GVQARRADVR HRRSILAEAQ VLQQKTEEGA VVFDALAQAW VGQATFPMPQ ATTAVNTVSD IHESCEEAAS AARTSQTLKL LTWNVMFDRY NNQPTPLGMP GIDFCTPQRY PILAKIIDQE DADVVGMQEV EPAFAEYLAA QPWCRERYIM SSSPRGPTLD PWGTLMLVRK GPESWPVQQL IYHNVPAWSG HVSLMPAVTL DLLGSRRQVS ARKSQQDPQQ AAGLLNVCTM HLLAPFVKAH ETARTAQDQA LRHCLTRQIH GDCVVMGDFN DWPSNEFSMP AETRYVECWP LVHPGDYGKT MDESNTFCKL KIEETFFGRS DKLFLRTGRP AQGAPPLLKP VEAHLVGTRS VNEENGNTAA PAYLFPSDHY GVSMTFDVLA RP

NT Sequence

ATGCGTGGCT TTCTGCGCGT CTCACCGCGA TGTCTCGTCT CCTACCGCGA CAACAGGCGT CTCATGGACG CCTACCACCT CCTCGTCACG AAGAAGCCGG TGGAGTACGA TTACGTGGCG ATCGACGTGG ACTCCTTCAT CAGCGGGGCA CAGCGGCTGG CCAAGAACGT CACCCCTGAG TCGCGCAGAA ACAAGGAGGT GGCCCGGCAT GTCGTCACCG CGCTGGAGCA GCTGCTGCGC CGCGTGGTGT GCAAGCAGTC TCTCCTGTTC GCCTTCGACG GCGCCGGGAT GGCGGCGAAG GTGCACGAGT ATCGGCACGG CCGCATCACT CGCAAGGTGG AGAGCCGCGC GGTTCGTCTG CCTGGGATGC CGCTCACACG AGCTGTGGAG GAGCGGCTGG TGCGGTCGAT GCCCCTCGCC AGCGGTCTCG TGCCCCGCGA GGTCGTTGTC TCCGGCACGA GCGTGGAGGG TGTGGTGGAG CGCAAGGCCG CGGCGTGGGC ACTGGACCTT GCCGCCCGTG ACGGGGCCCA GCACCACACC ACCCGCAGTC TCTGCTTTGT CGGCTCCAAC TCCCTCGTCC ACACACTCCT GGCCGCGACG CCGTACTTCC AGGCGACGCA CATCGTGCAC GGCTACGCAG ATTTGCGCCA CCTGACGCTG AACAATGCGC TGGCGTGGCT CGAGCTGGAT GGCGCGACGC GCAGTACGAC CAGCAGCAGC AGCAGCGGAA GCAGTGGGGG CGTTGACACA CGTGCGCTGA CCAGCGGCCG CCTTGATGCC CTGCTGCTGA TGCTCCTGTG CACCGGGTGC GAAGTCACGA GCCTGCCCGC GCTGGGCGGT GCGCAGTTCA ATCTGATGAT GGACGCCTAC CACAACAAGC GCGCTAGCGA GCGTGGCGAG GGGGCAACTG CGGCGGCGAC AGCAGCGCTG TCATCACCTG CCGTGCCGCG TGAGCCGTCC TTCCAGGTCG TGCGAGAGCT GCCGAACGGT GTGATGGAAG TCGATCTCGT CGCCCTTCTT CTTCTCTTTC TTTCCAAAGA TGCGGCAGCG GAGCTCTTGA GGTCGGCGGA GACGTTAACA ACCAAGGAGG CAGAGAAGTG TGTGCATGAC GCCCCCCTCT CCGTCGCCGC GCATGAGACC TACACGAACT TGATGGAGAG TTACCTCACC CACCTGTGGA GCACGCTCGT GCTGCTCTGC TACGGCACCA CCACGGCGGA GGCGATGCAG GTGCCCTTCT ACCTCACCCA GGGCGACGTG GGCACCTGTG CAGTGACGGT GCACCCCTTC GCTACCTATC TCCGCTCCGT GATTGCAAAA GGAAGGCGCT ACCGCCGCGT CAGTACTGCA TGCAGCACCA CAGGCACCAG CACAGACGCG GGACGCGGGC AGCCGGCGTA CGCGCCATTC TGGAGTCCAG GACAGCCATG GACCGCGGCG GAGTTCACCT TGCTGTGTCA GAACAAGCTG GTTGAAATGG AGCCGCTGCT GCGGGAGTAC CTACCTGGGA TCGCCACACA CCGCGTGCCA GAAATCGCGC GATCTCTCCT GTCCACGGAG CGCACCGTCG ATGCGGCGCG CGGCTTTCTG CAGCGGTGGT ACGCCACTGC CGACGCCGAC GCCGCCCACC CGTCTCTCTA CTTCAGCCCC TCGCTCTGCT GGCTTCGAAA CGAAAAGTCA CGCATGTGGG GCGTGCGTTA TGTGGACCTC GGGGTGCAGG CCCGCCGTGC CGATGTGCGC CACCGCCGCA GTATTTTGGC CGAGGCGCAG GTGCTGCAGC AGAAGACGGA GGAGGGTGCG GTGGTCTTTG ACGCGCTGGC GCAGGCGTGG GTGGGGCAGG CAACGTTTCC GATGCCACAG GCCACCACCG CTGTCAACAC CGTGAGCGAT ATCCACGAGA GCTGTGAAGA GGCCGCCTCT GCTGCACGCA CGTCGCAGAC GCTGAAGCTG CTCACGTGGA ATGTGATGTT TGACCGCTAC AACAACCAAC CCACCCCGCT CGGCATGCCC GGCATTGATT TCTGCACCCC GCAGCGCTAC CCAATTCTGG CAAAGATTAT CGACCAGGAG GACGCCGACG TGGTTGGGAT GCAAGAGGTA GAGCCGGCCT TCGCGGAGTA CCTCGCGGCG CAGCCGTGGT GCCGGGAGCG GTACATCATG TCCAGCAGTC CCCGTGGACC GACGCTGGAC CCGTGGGGGA CGCTGATGCT CGTGCGCAAG GGCCCGGAGT CGTGGCCGGT GCAGCAGCTC ATCTACCACA ACGTACCTGC GTGGTCCGGC CACGTCTCCC TCATGCCGGC GGTGACGCTG GATCTGTTGG GTAGCCGACG GCAGGTCAGC GCACGCAAGT CGCAGCAGGA CCCGCAGCAG GCTGCCGGGC TACTCAACGT GTGCACGATG CACCTTTTGG CGCCGTTTGT GAAGGCGCAT GAGACGGCAC GCACGGCGCA GGATCAGGCG CTGCGGCACT GCTTAACGCG ACAGATCCAC GGTGACTGTG TCGTCATGGG CGACTTCAAC GACTGGCCCA GCAATGAGTT CAGCATGCCA GCCGAGACGC GCTACGTGGA GTGCTGGCCG CTCGTCCACC CGGGCGACTA CGGCAAAACA ATGGACGAGT CGAACACTTT CTGCAAGCTC AAGATCGAGG AGACGTTCTT TGGCCGCTCC GACAAGCTTT TCTTGCGCAC CGGGAGGCCA GCGCAGGGGG CACCGCCGCT GCTGAAGCCG GTGGAGGCGC ACCTGGTGGG CACGCGCAGT GTGAACGAGG AGAACGGCAA CACCGCCGCC CCGGCCTACC TTTTTCCATC TGACCACTAT GGCGTGAGCA TGACCTTCGA CGTCCTCGCT CGTCCATAA

Details for CrfaA.19505.a.B2.GE41375

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	64kDa
OBSERVED MW:	66kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR GFLRVSPRCL VSYRDNRRLM DAYHLLVTKK PVEYDYVAID VDSFISGAQR LAKNVTPESR RNKEVARHVV TALEQLLRRV VCKQSLLFAF DGAGMAAKVH EYRHGRITRK VESRAVRLPG MPLTRAVEER LVRSMPLASG LVPREVVVSG TSVEGVVERK AAAWALDLAA RDGAQHHTTR SLCFVGSNSL VHTLLAATPY FQATHIVHGY ADLRHLTLNN ALAWLELDGA TRSTTSSSSS GSSGGVDTRA LTSGRLDALL LMLLCTGCEV TSLPALGGAQ FNLMMDAYHN KRASERGEGA TAAATAALSS PAVPREPSFQ VVRELPNGVM EVDLVALLLL FLSKDAAAEL LRSAETLTTK EAEKCVHDAP LSVAAHETYT NLMESYLTHL WSTLVLLCYG TTTAEAMQVP FYLTQGDVGT CAVTVHPFAT YLRSVIAKGR RYRRVSTACS TTGTSTDAGR GQPAYAPFWS PGQPWTAAEF TLLCQNKLVE MEPLLREYLP GIATHRVPEI ARSLLSTERT VDAARGFLQR WYATADADAA HPSLYFSPSL CWLRNEKSRM WGVRYVDLGV QA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg gctttctgcg cgtctcaccg cgatgtctcg tctcctaccg cgacaacagg cgtctcatgg acgcctacca cctcctcgtc acgaagaagc cggtggagta cgattacgtg gcgatcgacg tggactcctt catcagcggg gcacagcggc tggccaagaa cgtcacccct gagtcgcgca gaaacaagga ggtggcccgg catgtcgtca ccgcgctgga gcagctgctg cgccgcgtgg tgtgcaagca gtctctcctg ttcgccttcg acggcgccgg gatggcggcg aaggtgcacg agtatcggca cggccgcatc actcgcaagg tggagagccg cgcggttcgt ctgcctggga tgccgctcac acgagctgtg gaggagcggc tggtgcggtc gatgcccctc gccagcggtc tcgtgccccg cgaggtcgtt gtctccggca cgagcgtgga gggtgtggtg gagcgcaagg ccgcggcgtg ggcactggac cttgccgccc gtgacggggc ccagcaccac accacccgca gtctctgctt tgtcggctcc aactccctcg tccacacact cctggccgcg acgccgtact tccaggcgac gcacatcgtg cacggctacg cagatttgcg ccacctgacg ctgaacaatg cgctggcgtg gctcgagctg gatggcgcga cgcgcagtac gaccagcagc agcagcagcg gaagcagtgg gggcgttgac acacgtgcgc tgaccagcgg ccgccttgat gccctgctgc tgatgctcct gtgcaccggg tgcgaagtca cgagcctgcc cgcgctgggc ggtgcgcagt tcaatctgat gatggacgcc taccacaaca agcgcgctag cgagcgtggc gagggggcaa ctgcggcggc gacagcagcg ctgtcatcac ctgccgtgcc gcgtgagccg tccttccagg tcgtgcgaga gctgccgaac ggtgtgatgg aagtcgatct cgtcgccctt cttcttctct ttctttccaa agatgcggca gcggagctct tgaggtcggc ggagacgtta acaaccaagg aggcagagaa gtgtgtgcat gacgcccccc tctccgtcgc cgcgcatgag acctacacga acttgatgga gagttacctc acccacctgt ggagcacgct cgtgctgctc tgctacggca ccaccacggc ggaggcgatg caggtgccct tctacctcac ccagggcgac gtgggcacct gtgcagtgac ggtgcacccc ttcgctacct atctccgctc cgtgattgca aaaggaaggc gctaccgccg cgtcagtact gcatgcagca ccacaggcac cagcacagac gcgggacgcg ggcagccggc gtacgcgcca ttctggagtc caggacagcc atggaccgcg gcggagttca ccttgctgtg tcagaacaag ctggttgaaa tggagccgct gctgcgggag tacctacctg ggatcgccac acaccgcgtg ccagaaatcg cgcgatctct cctgtccacg gagcgcaccg tcgatgcggc gcgcggcttt ctgcagcggt ggtacgccac tgccgacgcc gacgccgccc acccgtctct ctacttcagc ccctcgctct gctggcttcg aaacgaaaag tcacgcatgt ggggcgtgcg ttatgtggac ctcggggtgc aggcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for CrfaA.19505.a.B3.GE41376

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHMTL KLLTWNVMFD RYNNQPTPLG MPGIDFCTPQ RYPILAKIID QEDADVVGMQ EVEPAFAEYL AAQPWCRERY IMSSSPRGPT LDPWGTLMLV RKGPESWPVQ QLIYHNVPAW SGHVSLMPAV TLDLLGSRRQ VSARKSQQDP QQAPGLLSVC TMHLLAPFVK AHETARTAQD QALRHCLTRQ IHGDCIVMGD FNDWPSNEFS MPAETRYVEC WPLVHPGDYG KTMDESNTFC KLKIEETFFG RSDKLFLRTG RPAQGAPPLL KPVEAHLVGT RSVNEENDNT AAPAYLFPSD HYGVSMXXXX LARP

Validated NT Sequence

atggctcacc accaccacca tatgacgctg aagctgctca cgtggaatgt gatgtttgac cgctacaaca accaacccac cccgctcggc atgcccggca ttgatttctg caccccgcag cgctacccaa ttctggcaaa gattatcgac caggaggacg ccgacgtggt tgggatgcaa gaggtagagc cggccttcgc ggagtacctc gcggcgcagc cgtggtgccg ggagcggtac atcatgtcca gcagtccccg tggaccgacg ctggacccgt gggggacgct gatgctcgtg cgcaagggcc cggagtcgtg gccggtgcag cagctcatct accacaacgt gcctgcgtgg tccggccacg tctccctcat gccggcggtg acgctggatc tgttaggtag ccgacggcag gtcagcgcac gcaagtcgca gcaggacccg cagcaggctc ccgggctact cagcgtgtgc acgatgcacc ttttagcgcc gtttgtgaaa gcgcatgaga cggcacgcac ggcgcaggat caggcgctgc ggcactgctt aacgcgacag atccacggtg actgtattgt catgggcgac ttcaacgact ggcccagtaa tgagttcagc atgccagccg agacgcgcta cgtggagtgc tggccgctcg tccacccggg cgactacggc aagacaatgg acgagtcgaa caccttctgc aagctcaaga tcgaagagac gttctttggc cgctccgaca agcttttctt gcgcaccggg aggccagcgc agggggcacc gccgctgctg aagccggtgg aggcgcacct ggtgggcacg cgcagtgtga acgaggagaa cgacaacacc gccgccccgg cctacctttt tccatctgac cactatggcg tgagcatgnn nnnnnnnnnc ctcgctcgtc cataagtga

Expected Protein Sequence

MAHHHHHHTL KLLTWNVMFD RYNNQPTPLG MPGIDFCTPQ RYPILAKIID QEDADVVGMQ EVEPAFAEYL AAQPWCRERY IMSSSPRGPT LDPWGTLMLV RKGPESWPVQ QLIYHNVPAW SGHVSLMPAV TLDLLGSRRQ VSARKSQQDP QQAAGLLNVC TMHLLAPFVK AHETARTAQD QALRHCLTRQ IHGDCVVMGD FNDWPSNEFS MPAETRYVEC WPLVHPGDYG KTMDESNTFC KLKIEETFFG RSDKLFLRTG RPAQGAPPLL KPVEAHLVGT RSVNEENGNT AAPAYLFPSD HYGVSMTFDV LARP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacgctga agctgctcac gtggaatgtg atgtttgacc gctacaacaa ccaacccacc ccgctcggca tgcccggcat tgatttctgc accccgcagc gctacccaat tctggcaaag attatcgacc aggaggacgc cgacgtggtt gggatgcaag aggtagagcc ggccttcgcg gagtacctcg cggcgcagcc gtggtgccgg gagcggtaca tcatgtccag cagtccccgt ggaccgacgc tggacccgtg ggggacgctg atgctcgtgc gcaagggccc ggagtcgtgg ccggtgcagc agctcatcta ccacaacgta cctgcgtggt ccggccacgt ctccctcatg ccggcggtga cgctggatct gttgggtagc cgacggcagg tcagcgcacg caagtcgcag caggacccgc agcaggctgc cgggctactc aacgtgtgca cgatgcacct tttggcgccg tttgtgaagg cgcatgagac ggcacgcacg gcgcaggatc aggcgctgcg gcactgctta acgcgacaga tccacggtga ctgtgtcgtc atgggcgact tcaacgactg gcccagcaat gagttcagca tgccagccga gacgcgctac gtggagtgct ggccgctcgt ccacccgggc gactacggca aaacaatgga cgagtcgaac actttctgca agctcaagat cgaggagacg ttctttggcc gctccgacaa gcttttcttg cgcaccggga ggccagcgca gggggcaccg ccgctgctga agccggtgga ggcgcacctg gtgggcacgc gcagtgtgaa cgaggagaac ggcaacaccg ccgccccggc ctaccttttt ccatctgacc actatggcgt gagcatgacc ttcgacgtcc tcgctcgtcc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat