CrfaA.19504.a

KREPB8

CENTER ID: CrfaA.19504.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.19504.a.B2.GE41063 ordered domain 63 417
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CFAC1_120009900

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPTLRCAAV VAANTSRVPN ALAKSVSDAV AFVPAHLVEG TRMEDAALIS PGSELKGCGT ECMSLFSHTP HEIFEGYSRQ AWIDTRRQFC HLCQEPVSCS PGTHCGDRDH TNLHFFLLLY AAYPRRDFLA EAVAAGTAGQ RAAKQQAMRM RDASPSLSAP SHGSPGRGQG SLEQALQALE QDAYLERRGE AGDHIAASRS NRKRQKPLWV SEKVLAGIQD VSPALHRFAT DHRTTDQLHT VDDAHRRSEL EALLFYLTHS PHQALPHALQ GTSNSGFWVS GERMWKSTIT GLISQIYPPL SAGMMTNFTQ KCWGRSNEER LYDALRLARI KSYYGWGPYA GKERKSFFMR QIIYELLLAE VRPGLSETGK LLAAEAVRRL AFELIFLQSM DYMHRIQHVY KLLGNPSFAE LRALRLL
NT Sequence
ATGCGACCGA CGCTGCGGTG TGCTGCTGTG GTGGCGGCGA ATACTTCGCG GGTGCCCAAC GCCTTGGCCA AGTCTGTTTC CGATGCTGTC GCCTTCGTGC CCGCCCACCT GGTGGAAGGA ACGCGGATGG AGGACGCCGC ACTCATCTCC CCGGGCTCGG AGCTGAAAGG CTGCGGCACG GAGTGCATGT CGCTCTTCTC CCACACGCCA CACGAAATTT TTGAAGGTTA CAGTCGCCAG GCGTGGATCG ACACACGGCG CCAGTTCTGC CACCTGTGTC AGGAGCCGGT CAGCTGCAGC CCTGGCACGC ACTGTGGCGA TCGCGACCAC ACGAATCTTC ACTTCTTTCT CCTCCTCTAC GCGGCCTACC CGCGCCGCGA CTTTCTGGCG GAGGCGGTGG CGGCGGGCAC GGCGGGGCAG CGGGCGGCGA AGCAGCAAGC AATGCGCATG CGCGACGCGT CGCCCTCTCT CTCCGCGCCG TCGCACGGCA GCCCAGGACG TGGTCAGGGG AGTCTCGAGC AGGCACTACA GGCGTTAGAG CAGGACGCGT ATTTGGAGAG AAGAGGTGAG GCGGGCGATC ATATTGCAGC ATCCCGCTCA AACAGGAAAC GTCAGAAGCC GCTGTGGGTG TCAGAGAAGG TGCTCGCAGG CATTCAGGAC GTCAGCCCCG CTCTGCACCG CTTCGCCACC GACCACCGCA CCACCGACCA GCTGCACACC GTCGACGACG CACATCGCCG CTCCGAGCTG GAGGCGCTGC TCTTCTACCT CACCCACTCG CCCCACCAGG CGCTGCCCCA CGCGCTTCAA GGTACCTCCA ACTCCGGCTT TTGGGTTAGC GGGGAGCGGA TGTGGAAGTC GACCATCACC GGACTCATCT CACAGATTTA CCCACCGCTG TCGGCTGGGA TGATGACCAA CTTCACCCAG AAGTGCTGGG GCCGCTCAAA TGAAGAACGC CTTTACGACG CCCTCCGCCT GGCACGGATC AAGTCCTACT ACGGCTGGGG GCCGTACGCG GGCAAGGAGC GGAAATCCTT CTTTATGCGG CAGATCATCT ACGAGCTCCT GCTGGCGGAG GTGCGGCCGG GTTTGTCGGA GACGGGGAAG CTGCTAGCGG CCGAGGCGGT GCGTCGTCTT GCCTTTGAGC TCATCTTTCT GCAGTCGATG GACTACATGC ACCGCATTCA GCACGTCTAC AAGCTGCTGG GAAACCCGTC CTTTGCGGAG CTGCGGGCAC TGAGGTTGCT TTAA
Details for CrfaA.19504.a.B2.GE41063
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS LFSHTPHEIF EGYSRQAWID TRRQFCHLCQ EPVSCSPGTH CGDRDHTNLH FFLLLYAAYP RRDFLAEAVA AGTAGQRAAK QQAMRMRDAS PSLSAPSHGS PGRGQGSLEQ ALQALEQDAY LERRGEAGDH IAASRSNRKR QKPLWVSEKV LAGIQDVSPA LHRFATDHRT TDQLHTVDDA HRRSELEALL FYLTHSPHQA LPHALQGTSN SGFWVSGERM WKSTITGLIS QIYPPLSAGM MTNFTQKCWG RSNEERLYDA LRLARIKSYY GWGPYAGKER KSFFMRQIIY ELLLAEVRPG LSETGKLLAA EAVRRLAFEL IFLQSMDYMH RIQHVYKLLG NPSFAELRAL RLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgc tcttctccca cacgccacac gaaatttttg aaggttacag tcgccaggcg tggatcgaca cacggcgcca gttctgccac ctgtgtcagg agccggtcag ctgcagccct ggcacgcact gtggcgatcg cgaccacacg aatcttcact tctttctcct cctctacgcg gcctacccgc gccgcgactt tctggcggag gcggtggcgg cgggcacggc ggggcagcgg gcggcgaagc agcaagcaat gcgcatgcgc gacgcgtcgc cctctctctc cgcgccgtcg cacggcagcc caggacgtgg tcaggggagt ctcgagcagg cactacaggc gttagagcag gacgcgtatt tggagagaag aggtgaggcg ggcgatcata ttgcagcatc ccgctcaaac aggaaacgtc agaagccgct gtgggtgtca gagaaggtgc tcgcaggcat tcaggacgtc agccccgctc tgcaccgctt cgccaccgac caccgcacca ccgaccagct gcacaccgtc gacgacgcac atcgccgctc cgagctggag gcgctgctct tctacctcac ccactcgccc caccaggcgc tgccccacgc gcttcaaggt acctccaact ccggcttttg ggttagcggg gagcggatgt ggaagtcgac catcaccgga ctcatctcac agatttaccc accgctgtcg gctgggatga tgaccaactt cacccagaag tgctggggcc gctcaaatga agaacgcctt tacgacgccc tccgcctggc acggatcaag tcctactacg gctgggggcc gtacgcgggc aaggagcgga aatccttctt tatgcggcag atcatctacg agctcctgct ggcggaggtg cggccgggtt tgtcggagac ggggaagctg ctagcggccg aggcggtgcg tcgtcttgcc tttgagctca tctttctgca gtcgatggac tacatgcacc gcattcagca cgtctacaag ctgctgggaa acccgtcctt tgcggagctg cgggcactga ggttgctttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat