SSGCID: Target Page

CrfaA.19502.a

KREPA1

CENTER ID:	CrfaA.19502.a
ORGANISM:	Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrfaA.19502.a.B1.GE40822	full length		1	938

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CFAC1_160014900

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLPQCRCTSA RTASTAWRRR LSHSGHRIAL RSGTLAAAAL ALGVSTASVG RPTRLLRQST TNNNATASPA AAGSTASAEV SAPPPAASTM SCYDTFLFSQ DDLHLRLLQE AHRKHGVWLT VPPQLAPTVP NVGPPAIPEP MYPPRQRERF EALERGAAEA RRARGSRSTT YAPTDVLADP NCLAYAPTHL PPPFITTGTH NFRMAGGDVA DVHVAGADAT QAAAGAVAVH ATASAVMMEV AVRAPVDASM LFHCSACGRA FRRREAAEAH VRQRHDNTGK SADAAARVVE GGGPGEITGY KTVLGSPSHP TGASRANISS AANQPRGVVC TDAAAAKSAT EASTTTAAAA TAPAEETKAP AADTAEFTAM PLPPPAALLR KRGESLVSAA YRAAPAVHLP EEHLVDELLA SVWDDVALHR EDIEKAGKSS TSPSAAGEAV PATAVDSCNG RFFIPSSSFV EGKADNRAEL EAELAGKTLA RATPAGAAPG IKRRTVNVTP ATVAARRLMV ARLGEDGNVD AAAAVASAAP TAQELSITEL SRHYPNPFGD SPNAALIEQE KEPANPFRDL EAEGEREKGE GEAGTAAADA TATPEQRWST WQQRFAARPY ACPLCQRNEL PQLAALLTGS STTAAGTAGA GAAAASASPP TPSSPAAPCT ASLADEVAWR QYETRVPRFR LLDSLDNHIE SCHAEGNPGS SSGEVTEEEW QRLYAVEASP TDAAARELRA VQLAVRVKEG SATTNGTATA AARRAATSSS AATSGKSSSA SSATASEATV TEDGGENSTE KADEDAAATT ADGARDALAG AEAPHVHVRA AVNTVLVGEV RDVQEGFVGS MRVLQYVVAV HNGSPCSASE GGEEKEEEAV ADEDLIVVRC MGDLVPVALL HQKVQLGSTL FVAGSLRMNR SIDTASRRSH AYPYVQVVPP LGSVRVVK

NT Sequence

atgctgccac agtgccgatg cacgtctgca cgcaccgcct ccacagcttg gaggcggcgg ctctcacaca gcggccaccg cattgcgctc cgctccggca ctctcgctgc tgctgctctg gctcttggcg tctcgacagc ttctgtaggg cgtcccaccc gcctcctacg gcagtcgaca accaacaaca acgcgactgc ttcacctgcc gcagcagggt ccacggcatc agcggaggtt tccgcaccgc cgccggcagc cagcaccatg agctgctacg acacgtttct cttttctcaa gacgatctgc atttgcgact gctgcaggag gcacaccgca agcacggggt gtggctcacc gtcccaccac agcttgcccc tacggtgccc aacgtgggtc ccccagccat tccggagccc atgtacccac ccaggcagcg agagcgcttc gaggcgttgg agcgcggggc cgccgaggcg cggcgcgcgc gtgggtcacg ctccaccacc tacgccccca ctgacgtgct ggcagaccca aactgcctcg cctacgcacc aacgcacctg cccccaccct tcatcacaac cggaacgcat aactttcgga tggcaggagg tgacgtggca gatgtgcacg ttgcaggtgc tgatgctacg caggctgccg caggtgctgt tgcggtgcac gcgacggcgt cggcggtgat gatggaggtc gccgtgcgtg ccccggttga cgcgtcgatg ctgttccact gcagcgcgtg tgggcgggcc tttcggcgcc gggaggcggc ggaggcacac gtgcggcagc gccacgacaa caccgggaag tcggctgacg ctgcggcgag ggtggtagag ggcggagggc cgggcgagat tacaggctac aagacagtgc tcggctcccc ctctcatccc accggtgctt cgcgggcgaa catctcgtct gcggcgaacc agcctcgagg ggtggtatgt accgatgccg cggctgccaa gtcggcgacg gaggccagca ccaccaccgc tgccgctgcg accgcgcctg ccgaggagac gaaggcaccc gcggcggaca cagcagaatt caccgcgatg ccgctgccgc cgccggccgc gctgctgcgt aagagagggg agagtttggt cagcgccgcc taccgggctg cgccggcggt gcacctgccg gaggagcacc tcgtagacga gctgttggcc tccgtctggg atgacgtggc gctgcaccgc gaagacatcg agaaggctgg caagagctcc acctcgcctt ccgctgctgg tgaggcggtg ccggcgacgg cggtggacag ctgcaacggc cgcttcttca ttcccagctc ttccttcgtg gaaggcaagg cggacaaccg tgcagagctc gaggcggagt tggctggcaa gacgctcgcg cgggccacgc cggcaggtgc cgctcccgga atcaagcgca ggacagtgaa cgtgacaccc gccaccgttg cggcgcgtcg tctcatggtt gcccgcctcg gcgaggatgg caatgtcgac gcggcggctg ccgtggcgtc ggccgctccc acggcgcagg agctgagcat cacggagttg tcgcggcact acccgaaccc gttcggcgac agcccgaatg ctgctctcat agagcaggag aaggagccgg cgaacccttt ccgggacttg gaggccgagg gggagaggga gaagggcgag ggagaggcgg ggacggcagc agcagacgcg accgcgacac cggagcagcg gtggtcgacg tggcagcagc gcttcgctgc ccgcccttat gcctgcccgc tgtgtcagcg gaacgaactg ccgcagctcg cggctctcct caccggcagt agcactactg ctgctggtac tgctggtgct ggtgctgctg ctgcctctgc atccccgcca accccctcgt cgccggcggc accctgcaca gcttcactgg cagatgaggt ggcgtggagg cagtatgaga ctcgcgtccc acgatttcgc ctactcgact cgctggacaa ccacatcgag tcgtgccacg cggagggcaa ccccggcagt agcagtgggg aggtgacgga ggaagagtgg cagcggctct acgcggtgga ggcgtcgccg acagacgcag cggcacggga gctgcgtgca gtgcagctgg ctgttcgggt aaaagagggt agtgccacca ctaacggcac tgccaccgca gcagcacggc gcgcggcgac ttcgtcctcg gcggcaacga gtgggaagtc aagcagcgct tcctccgcta ccgcatccga ggcaacggtg actgaggatg ggggcgagaa cagcacggag aaggcggacg aggacgccgc cgcaacaacc gcggacggcg cgcgcgatgc cctcgccgga gcagaggccc cacacgtgca cgtccgcgct gcggtgaaca ctgttctcgt cggcgaggtg cgcgatgtac aggagggctt cgtggggagc atgcgtgtgc tgcagtacgt cgtggcggtt cacaatggca gcccgtgcag cgcgagcgag ggtggcgagg agaaggagga ggaggcagtg gcagatgaag atttgattgt tgtgcggtgc atgggcgacc ttgttcctgt cgcgctgctt catcaaaagg tgcagctcgg ctccacgctg tttgttgcgg gatcgttgcg tatgaaccgc agcattgaca cggcgtcgcg ccgctcgcat gcgtacccgt atgtgcaggt ggttccaccc ctcggttccg ttcgcgtcgt caagtaa

Details for CrfaA.19502.a.B1.GE40822

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	98kDa
OBSERVED MW:	98kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML PQCRCTSART ASTAWRRRLS HSGHRIALRS GTLAAAALAL GVSTASVGRP TRLLRQSTTN NNATASPAAA GSTASAEVSA PPPAASTMSC YDTFLFSQDD LHLRLLQEAH RKHGVWLTVP PQLAPTVPNV GPPAIPEPMY PPRQRERFEA LERGAAEARR ARGSRSTTYA PTDVLADPNC LAYAPTHLPP PFITTGTHNF RMAGGDVADV HVAGADATQA AAGAVAVHAT ASAVMMEVAV RAPVDASMLF HCSACGRAFR RREAAEAHVR QRHDNTGKSA DAAARVVEGG GPGEITGYKT VLGSPSHPTG ASRANISSAA NQPRGVVCTD AAAAKSATEA STTTAAAATA PAEETKAPAA DTAEFTAMPL PPPAALLRKR GESLVSAAYR AAPAVHLPEE HLVDELLASV WDDVALHRED IEKAGKSSTS PSAAGEAVPA TAVDSCNGRF FIPSSSFVEG KADNRAELEA ELAGKTLARA TPAGAAPGIK RRTVNVTPAT VAARRLMVAR LGEDGNVDAA AAVASAAPTA QELSITELSR HYPNPFGDSP NAALIEQEKE PANPFRDLEA EGEREKGEGE AGTAAADATA TPEQRWSTWQ QRFAARPYAC PLCQRNELPQ LAALLTGSST TAAGTAGAGA AAASASPPTP SSPAAPCTAS LADEVAWRQY ETRVPRFRLL DSLDNHIESC HAEGNPGSSS GEVTEEEWQR LYAVEASPTD AAARELRAVQ LAVRVKEGSA TTNGTATAAA RRAATSSSAA TSGKSSSASS ATASEATVTE DGGENSTEKA DEDAAATTAD GARDALAGAE APHVHVRAAV NTVLVGEVRD VQEGFVGSMR VLQYVVAVHN GSPCSASEGG EEKEEEAVAD EDLIVVRCMG DLVPVALLHQ KVQLGSTLFV AGSLRMNRSI DTASRRSHAY PYVQVVPPLG SVRVVK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc cacagtgccg atgcacgtct gcacgcaccg cctccacagc ttggaggcgg cggctctcac acagcggcca ccgcattgcg ctccgctccg gcactctcgc tgctgctgct ctggctcttg gcgtctcgac agcttctgta gggcgtccca cccgcctcct acggcagtcg acaaccaaca acaacgcgac tgcttcacct gccgcagcag ggtccacggc atcagcggag gtttccgcac cgccgccggc agccagcacc atgagctgct acgacacgtt tctcttttct caagacgatc tgcatttgcg actgctgcag gaggcacacc gcaagcacgg ggtgtggctc accgtcccac cacagcttgc ccctacggtg cccaacgtgg gtcccccagc cattccggag cccatgtacc cacccaggca gcgagagcgc ttcgaggcgt tggagcgcgg ggccgccgag gcgcggcgcg cgcgtgggtc acgctccacc acctacgccc ccactgacgt gctggcagac ccaaactgcc tcgcctacgc accaacgcac ctgcccccac ccttcatcac aaccggaacg cataactttc ggatggcagg aggtgacgtg gcagatgtgc acgttgcagg tgctgatgct acgcaggctg ccgcaggtgc tgttgcggtg cacgcgacgg cgtcggcggt gatgatggag gtcgccgtgc gtgccccggt tgacgcgtcg atgctgttcc actgcagcgc gtgtgggcgg gcctttcggc gccgggaggc ggcggaggca cacgtgcggc agcgccacga caacaccggg aagtcggctg acgctgcggc gagggtggta gagggcggag ggccgggcga gattacaggc tacaagacag tgctcggctc cccctctcat cccaccggtg cttcgcgggc gaacatctcg tctgcggcga accagcctcg aggggtggta tgtaccgatg ccgcggctgc caagtcggcg acggaggcca gcaccaccac cgctgccgct gcgaccgcgc ctgccgagga gacgaaggca cccgcggcgg acacagcaga attcaccgcg atgccgctgc cgccgccggc cgcgctgctg cgtaagagag gggagagttt ggtcagcgcc gcctaccggg ctgcgccggc ggtgcacctg ccggaggagc acctcgtaga cgagctgttg gcctccgtct gggatgacgt ggcgctgcac cgcgaagaca tcgagaaggc tggcaagagc tccacctcgc cttccgctgc tggtgaggcg gtgccggcga cggcggtgga cagctgcaac ggccgcttct tcattcccag ctcttccttc gtggaaggca aggcggacaa ccgtgcagag ctcgaggcgg agttggctgg caagacgctc gcgcgggcca cgccggcagg tgccgctccc ggaatcaagc gcaggacagt gaacgtgaca cccgccaccg ttgcggcgcg tcgtctcatg gttgcccgcc tcggcgagga tggcaatgtc gacgcggcgg ctgccgtggc gtcggccgct cccacggcgc aggagctgag catcacggag ttgtcgcggc actacccgaa cccgttcggc gacagcccga atgctgctct catagagcag gagaaggagc cggcgaaccc tttccgggac ttggaggccg agggggagag ggagaagggc gagggagagg cggggacggc agcagcagac gcgaccgcga caccggagca gcggtggtcg acgtggcagc agcgcttcgc tgcccgccct tatgcctgcc cgctgtgtca gcggaacgaa ctgccgcagc tcgcggctct cctcaccggc agtagcacta ctgctgctgg tactgctggt gctggtgctg ctgctgcctc tgcatccccg ccaaccccct cgtcgccggc ggcaccctgc acagcttcac tggcagatga ggtggcgtgg aggcagtatg agactcgcgt cccacgattt cgcctactcg actcgctgga caaccacatc gagtcgtgcc acgcggaggg caaccccggc agtagcagtg gggaggtgac ggaggaagag tggcagcggc tctacgcggt ggaggcgtcg ccgacagacg cagcggcacg ggagctgcgt gcagtgcagc tggctgttcg ggtaaaagag ggtagtgcca ccactaacgg cactgccacc gcagcagcac ggcgcgcggc gacttcgtcc tcggcggcaa cgagtgggaa gtcaagcagc gcttcctccg ctaccgcatc cgaggcaacg gtgactgagg atgggggcga gaacagcacg gagaaggcgg acgaggacgc cgccgcaaca accgcggacg gcgcgcgcga tgccctcgcc ggagcagagg ccccacacgt gcacgtccgc gctgcggtga acactgttct cgtcggcgag gtgcgcgatg tacaggaggg cttcgtgggg agcatgcgtg tgctgcagta cgtcgtggcg gttcacaatg gcagcccgtg cagcgcgagc gagggtggcg aggagaagga ggaggaggca gtggcagatg aagatttgat tgttgtgcgg tgcatgggcg accttgttcc tgtcgcgctg cttcatcaaa aggtgcagct cggctccacg ctgtttgttg cgggatcgtt gcgtatgaac cgcagcattg acacggcgtc gcgccgctcg catgcgtacc cgtatgtgca ggtggttcca cccctcggtt ccgttcgcgt cgtcaagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at