CrfaA.19501.a

JBP3 (glucosyl transferase interacting protein 2) (GTIP2)

CENTER ID: CrfaA.19501.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.19501.a.B1.GE40821 full length 1 657
CrfaA.19501.a.B2.GE40652 C-term domain 370 644
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79386
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:CFAC1_250010700
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAANRNAYPS GPRQGEELEP RESSTSSSVS TDSSTDSDDS SSGGSDSSSS VSSSTPRNAA GDAAARPASS ATSGGASQNQ RFVHLNPDLR FERPDNEYIP LPPQRDLQQV STVLRRICVD KQRYPEGRHR MLDYFRVAVE MFVTEVRREY QQQYANAQRN GKVMQRFTWK NNGELAICYA CCCDMLKLLY DSIQPGPLKP LWDSFVGQLP PLLIVESRVP VSILSEQTYH VKYMDWQKGG TRYAGDQHTA NVRFPSAKDR RLKIEAYLKS GSGFRLAVLT QHVAVATSAA ATSASPTSAQ PPPTARAAEE PRPPFAGRLP GSAGVKVPLA PGVTVARPPV KMPMKQAAGA HGAAQANAAA PLASTGVKQM PPEMKHLFWL SVLAHVKGGE ATRAFPDERL EALPGLLRLC EMTEEAAEMR ILMDVLAASS GGVQTVFERL GGLLSFRRFA AHFIQRGDAD ALLQLVEQIT RWRVAAWGST SQQAWQTNMD QKRTPDLSWL RTLPLIPGAK RAAWGALLVV LEQRFIFRAP RPDDIGGAGV KRPRPEGQVG DLRAVLSDNV DMQKWVVPRV NVFSAVRLPV ELRVPPLCTK LEEDTIRFLN AKRSYAVSTQ REWCERLGKV LAASSVGQEG SHVPVWYDPY ELLSVAVPDT VEGKEHA
NT Sequence
atggccgcca accgcaacgc atatcccagc ggccccaggc aaggggagga gctggagccg agggagtctt cgacatcgag cagtgtgtcg accgactcca gcaccgacag cgacgactcc agctctggcg gctctgacag cagctcgtcc gtcagctcct ccactccccg caacgctgcc ggcgacgctg cggcccggcc ggcatcctca gccacgtctg gtggtgcgtc gcagaatcag cgcttcgtcc acctcaaccc cgacctccgc tttgagcggc ccgacaacga gtacattccg ctgcccccgc agcgcgacct gcagcaggtg tccacggtgc tgcggcgcat ttgcgtcgac aagcagcgct accccgaggg ccggcaccgc atgctcgact acttccgggt ggctgtcgag atgttcgtga cggaggtgcg gcgggagtat cagcagcagt acgccaacgc gcagcgcaac ggcaaggtga tgcagcgctt cacgtggaag aacaacgggg agctggccat ctgctacgcc tgctgctgcg acatgctgaa gcttctctac gactcgattc agccaggccc cttgaagccg ctctgggaca gcttcgtggg gcagctgccg cccctgctaa ttgtggagag ccgcgtgccg gtcagcatcc tgtcggagca gacgtaccac gtgaagtaca tggactggca gaagggcggg acgcgttacg ccggcgatca gcacacggca aacgtccgct ttccctccgc caaggaccgg cgattgaaga ttgaggcgta cttgaagagc ggctcgggct tccgcctggc ggtgctcacg cagcacgtcg cagtcgccac cagcgcggcc gccacgagcg cctcgccgac gagtgcgcag ccgccgccca ccgcgcgtgc agcggaggag ccgcggccgc ccttcgccgg tcgtctgccc ggcagcgccg gcgtgaaggt gccgctcgca cccggggtga cggtggcccg tccgcccgtg aagatgccga tgaagcaggc ggctggtgcc cacggcgcgg cgcaggcgaa cgctgccgcc ccactggcct ccaccggggt gaagcagatg cctccggaga tgaagcacct cttctggctc tctgttctcg cccacgtgaa gggtggcgaa gccactcgcg cctttccaga cgagcggctg gaggcactgc ccggcctgct gcggctgtgc gagatgacag aggaggcggc ggagatgcgc atcttgatgg acgtcctcgc cgcctccagc ggtggggtgc agacggtctt tgagcgcctc ggcgggctgc tgtccttccg gcgcttcgcc gcccacttca tccagcgcgg cgacgccgac gcgctgctgc agctcgtgga gcaaatcacg cggtggcgcg ttgcggcgtg gggcagcacc tcccagcagg cgtggcagac gaacatggac cagaagcgca cacccgacct gtcgtggctg cggacgttgc ctctcatacc gggggcgaag cgggcggcgt ggggagcgct gctggtggtg cttgagcagc gcttcatctt ccgcgctcct cgcccggatg acatcggcgg cgccggagta aagcggccgc ggccggaagg gcaggttgga gacctgcgcg ctgtgctgag cgacaacgtc gacatgcaga agtgggtggt gccgcgcgtg aatgttttct cggcggtgcg gctgccggtg gagttgcgcg tgccgccgct gtgcacgaag ctggaggagg acacgattcg ctttctcaac gcaaagcgca gctacgccgt gagtacgcag cgggagtggt gtgagcggct ggggaaggtg ctggctgcca gctcggttgg gcaggaggga tcgcatgtgc ctgtctggta tgacccgtac gagctgctca gtgtggcggt ccctgatacc gtagagggca aagagcacgc gtga
Details for CrfaA.19501.a.B1.GE40821
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 73kDa
OBSERVED MW: 73kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA ANRNAYPSGP RQGEELEPRE SSTSSSVSTD SSTDSDDSSS GGSDSSSSVS SSTPRNAAGD AAARPASSAT SGGASQNQRF VHLNPDLRFE RPDNEYIPLP PQRDLQQVST VLRRICVDKQ RYPEGRHRML DYFRVAVEMF VTEVRREYQQ QYANAQRNGK VMQRFTWKNN GELAICYACC CDMLKLLYDS IQPGPLKPLW DSFVGQLPPL LIVESRVPVS ILSEQTYHVK YMDWQKGGTR YAGDQHTANV RFPSAKDRRL KIEAYLKSGS GFRLAVLTQH VAVATSAAAT SASPTSAQPP PTARAAEEPR PPFAGRLPGS AGVKVPLAPG VTVARPPVKM PMKQAAGAHG AAQANAAAPL ASTGVKQMPP EMKHLFWLSV LAHVKGGEAT RAFPDERLEA LPGLLRLCEM TEEAAEMRIL MDVLAASSGG VQTVFERLGG LLSFRRFAAH FIQRGDADAL LQLVEQITRW RVAAWGSTSQ QAWQTNMDQK RTPDLSWLRT LPLIPGAKRA AWGALLVVLE QRFIFRAPRP DDIGGAGVKR PRPEGQVGDL RAVLSDNVDM QKWVVPRVNV FSAVRLPVEL RVPPLCTKLE EDTIRFLNAK RSYAVSTQRE WCERLGKVLA ASSVGQEGSH VPVWYDPYEL LSVAVPDTVE GKEHA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg ccaaccgcaa cgcatatccc agcggcccca ggcaagggga ggagctggag ccgagggagt cttcgacatc gagcagtgtg tcgaccgact ccagcaccga cagcgacgac tccagctctg gcggctctga cagcagctcg tccgtcagct cctccactcc ccgcaacgct gccggcgacg ctgcggcccg gccggcatcc tcagccacgt ctggtggtgc gtcgcagaat cagcgcttcg tccacctcaa ccccgacctc cgctttgagc ggcccgacaa cgagtacatt ccgctgcccc cgcagcgcga cctgcagcag gtgtccacgg tgctgcggcg catttgcgtc gacaagcagc gctaccccga gggccggcac cgcatgctcg actacttccg ggtggctgtc gagatgttcg tgacggaggt gcggcgggag tatcagcagc agtacgccaa cgcgcagcgc aacggcaagg tgatgcagcg cttcacgtgg aagaacaacg gggagctggc catctgctac gcctgctgct gcgacatgct gaagcttctc tacgactcga ttcagccagg ccccttgaag ccgctctggg acagcttcgt ggggcagctg ccgcccctgc taattgtgga gagccgcgtg ccggtcagca tcctgtcgga gcagacgtac cacgtgaagt acatggactg gcagaagggc gggacgcgtt acgccggcga tcagcacacg gcaaacgtcc gctttccctc cgccaaggac cggcgattga agattgaggc gtacttgaag agcggctcgg gcttccgcct ggcggtgctc acgcagcacg tcgcagtcgc caccagcgcg gccgccacga gcgcctcgcc gacgagtgcg cagccgccgc ccaccgcgcg tgcagcggag gagccgcggc cgcccttcgc cggtcgtctg cccggcagcg ccggcgtgaa ggtgccgctc gcacccgggg tgacggtggc ccgtccgccc gtgaagatgc cgatgaagca ggcggctggt gcccacggcg cggcgcaggc gaacgctgcc gccccactgg cctccaccgg ggtgaagcag atgcctccgg agatgaagca cctcttctgg ctctctgttc tcgcccacgt gaagggtggc gaagccactc gcgcctttcc agacgagcgg ctggaggcac tgcccggcct gctgcggctg tgcgagatga cagaggaggc ggcggagatg cgcatcttga tggacgtcct cgccgcctcc agcggtgggg tgcagacggt ctttgagcgc ctcggcgggc tgctgtcctt ccggcgcttc gccgcccact tcatccagcg cggcgacgcc gacgcgctgc tgcagctcgt ggagcaaatc acgcggtggc gcgttgcggc gtggggcagc acctcccagc aggcgtggca gacgaacatg gaccagaagc gcacacccga cctgtcgtgg ctgcggacgt tgcctctcat accgggggcg aagcgggcgg cgtggggagc gctgctggtg gtgcttgagc agcgcttcat cttccgcgct cctcgcccgg atgacatcgg cggcgccgga gtaaagcggc cgcggccgga agggcaggtt ggagacctgc gcgctgtgct gagcgacaac gtcgacatgc agaagtgggt ggtgccgcgc gtgaatgttt tctcggcggt gcggctgccg gtggagttgc gcgtgccgcc gctgtgcacg aagctggagg aggacacgat tcgctttctc aacgcaaagc gcagctacgc cgtgagtacg cagcgggagt ggtgtgagcg gctggggaag gtgctggctg ccagctcggt tgggcaggag ggatcgcatg tgcctgtctg gtatgacccg tacgagctgc tcagtgtggc ggtccctgat accgtagagg gcaaagagca cgcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for CrfaA.19501.a.B2.GE40652
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP PEMKHLFWLS VLAHVKGGEA TRAFPDERLE ALPGLLRLCE MTEEAAEMRI LMDVLAASSG GVQTVFERLG GLLSFRRFAA HFIQRGDADA LLQLVEQITR WRVAAWGSTS QQAWQTNMDQ KRTPDLSWLR TLPLIPGAKR AAWGALLVVL EQRFIFRAPR PDDVGGAGVK RPRPEGQVGD LRAVLSDNVD MQKWVVPRVN VFSAVRLPVE LRVPPLCTKL EEDTIRFLNA KRSYAVSTQR EWCERLGKVL AASSVGQEGS HVPVWYDPYE LLS
Validated NT Sequence
atggctcacc accaccacca ccatatgcct ccggagatga agcacctctt ctggctctct gttctcgccc acgtgaaggg cggcgaggcc acgcgcgcct ttccagacga gcggctggag gcgctgcccg gcctgctgcg gctgtgcgag atgacagagg aggcggcgga gatgcgcatc ttgatggacg tcctcgccgc ctccagcggt ggggtgcaga cggtctttga gcgcctcggc gggctgctgt ccttccggcg cttcgccgcc cacttcatcc agcgcggcga cgccgacgcg ctgctgcagc tcgtggagca aatcacgcgg tggcgcgttg cggcgtgggg cagcacctcc cagcaggcgt ggcagacgaa catggaccag aagcgcacac ccgacctgtc gtggctgcgg acgttgcctc tcataccggg ggcgaagcgg gcggcgtggg gagcgctgct ggtggtgctt gagcagcgct tcatcttccg cgctcctcgc ccggatgacg tcggcggcgc cggggtgaag cggccgcggc cggaagggca ggttggggac ctgcgcgctg tgctgagcga caacgtcgac atgcagaagt gggtggtgcc gcgcgtgaat gtcttctcgg cggtgcggct gccggtagag ttgcgcgtgc cgccgctgtg cacgaagcta gaggaggaca cgattcgctt tctcaacgca aagcgcagct acgcggtgag tacgcagcgg gagtggtgtg agcggctggg gaaggtgctg gctgccagct cggttgggca ggagggatcg catgtgcctg tctggtatga cccntacgag ctgctcagtt aagtg
Expected Protein Sequence
MAHHHHHHMP PEMKHLFWLS VLAHVKGGEA TRAFPDERLE ALPGLLRLCE MTEEAAEMRI LMDVLAASSG GVQTVFERLG GLLSFRRFAA HFIQRGDADA LLQLVEQITR WRVAAWGSTS QQAWQTNMDQ KRTPDLSWLR TLPLIPGAKR AAWGALLVVL EQRFIFRAPR PDDIGGAGVK RPRPEGQVGD LRAVLSDNVD MQKWVVPRVN VFSAVRLPVE LRVPPLCTKL EEDTIRFLNA KRSYAVSTQR EWCERLGKVL AASSVGQEGS HVPVWYDPYE LLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctc cggagatgaa gcacctcttc tggctctctg ttctcgccca cgtgaagggt ggcgaagcca ctcgcgcctt tccagacgag cggctggagg cactgcccgg cctgctgcgg ctgtgcgaga tgacagagga ggcggcggag atgcgcatct tgatggacgt cctcgccgcc tccagcggtg gggtgcagac ggtctttgag cgcctcggcg ggctgctgtc cttccggcgc ttcgccgccc acttcatcca gcgcggcgac gccgacgcgc tgctgcagct cgtggagcaa atcacgcggt ggcgcgttgc ggcgtggggc agcacctccc agcaggcgtg gcagacgaac atggaccaga agcgcacacc cgacctgtcg tggctgcgga cgttgcctct cataccgggg gcgaagcggg cggcgtgggg agcgctgctg gtggtgcttg agcagcgctt catcttccgc gctcctcgcc cggatgacat cggcggcgcc ggagtaaagc ggccgcggcc ggaagggcag gttggagacc tgcgcgctgt gctgagcgac aacgtcgaca tgcagaagtg ggtggtgccg cgcgtgaatg ttttctcggc ggtgcggctg ccggtggagt tgcgcgtgcc gccgctgtgc acgaagctgg aggaggacac gattcgcttt ctcaacgcaa agcgcagcta cgccgtgagt acgcagcggg agtggtgtga gcggctgggg aaggtgctgg ctgccagctc ggttgggcag gagggatcgc atgtgcctgt ctggtatgac ccgtacgagc tgctcagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat