CrfaA.18236.a

KREL2

CENTER ID: CrfaA.18236.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.18236.a.B1.GE40820 full length 1 422
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CFAC1_170031000

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRCQLLRL LRRSPLLRAD EPKDAVFERY SEIENSNMRR ITALRGIGLF NDEWIATEKV HGANFGIYST DHGKAIRYAK RSGIMPPSEH FFGYHILLPD LTRYAQKGRE LLTSQLKKSP SSIVINGELF GGKYDHPNLQ KKRETVIVGG KPRAISAVQN DPFPQYSPSL HFYAFDMKYK LADADTEYTT LTYDEATAIF EAIPGLLYAK AIIRGPLSKV AAFNVETFTT TIPPLVGMGD YPLRGNWAEG LVVKHCKRGK PGFAPNGHTI LKFKSTAFQE ISTDRQQGPR VDEMEEVRRQ SIQISGVQLP DLESVIRDAE VRAATLHLLD HVCDNRMKSV LSKIGTEPFE SQTMTPDELA TLLAKDALKD FLKEAEPKVV NSPLLLRREI TRYVLFESRK YVASKWKEIV SRQKDADSDA AQ
NT Sequence
ATGTTGCGGC GCTGCCAGCT GCTGCGTCTG CTCCGGCGGT CGCCGCTCCT GCGGGCCGAT GAGCCCAAGG ATGCTGTTTT CGAGCGCTAC AGCGAAATTG AAAACTCGAA CATGCGGCGC ATCACCGCGC TGAGAGGCAT CGGACTTTTC AACGACGAGT GGATCGCAAC CGAAAAGGTG CACGGCGCCA ACTTCGGCAT CTACTCCACC GACCACGGCA AGGCGATCCG TTACGCGAAG CGGAGTGGCA TCATGCCGCC CAGCGAACAC TTTTTTGGTT ACCACATCTT GCTTCCGGAC CTCACGCGGT ACGCGCAGAA GGGCCGCGAG TTGTTGACGA GCCAGCTGAA AAAGTCGCCC AGCAGCATCG TGATCAACGG CGAGCTCTTT GGGGGGAAGT ACGACCACCC GAACCTGCAG AAGAAGCGAG AGACGGTGAT CGTGGGTGGA AAGCCGCGCG CCATATCCGC CGTGCAGAAC GATCCTTTTC CGCAGTACAG CCCCAGTCTT CACTTCTACG CCTTCGATAT GAAGTACAAG CTGGCTGACG CAGACACCGA GTACACCACC TTGACGTACG ACGAGGCAAC CGCCATTTTC GAAGCCATCC CCGGACTGCT CTACGCCAAG GCCATCATCC GCGGGCCCCT CAGCAAGGTG GCCGCCTTCA ACGTCGAGAC CTTCACGACA ACGATTCCAC CGCTCGTCGG CATGGGCGAC TACCCGCTGC GCGGCAACTG GGCAGAGGGC CTCGTGGTGA AGCACTGCAA GCGCGGCAAG CCCGGCTTCG CCCCCAACGG TCACACGATC CTGAAGTTCA AGTCCACTGC CTTTCAAGAA ATTTCCACCG ACCGCCAGCA GGGCCCGCGG GTGGACGAGA TGGAAGAGGT GCGGCGCCAG TCGATCCAAA TCTCCGGCGT GCAGCTGCCA GATCTGGAGA GCGTCATCCG GGACGCCGAG GTGCGGGCGG CGACGCTGCA CCTGCTCGAC CACGTCTGCG ACAACCGCAT GAAGAGCGTG CTGTCAAAGA TCGGCACGGA GCCGTTCGAG TCGCAGACGA TGACGCCCGA TGAACTGGCG ACGCTGCTCG CGAAGGATGC TCTGAAGGAC TTTTTAAAGG AAGCCGAGCC GAAGGTGGTG AACTCGCCTC TGCTGCTGCG ACGCGAGATT ACGCGTTACG TCTTGTTCGA GTCTCGCAAG TACGTTGCGA GTAAGTGGAA GGAGATCGTC TCTCGGCAGA AGGATGCAGA CAGCGACGCG GCACAGTAA
Details for CrfaA.18236.a.B1.GE40820
HARVESTED ON: data unavailable
SEQUENCED ON: 12/6/2016
EXPECTED MW: 49kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML RRCQLLRLLR RSPLLRADEP KDAVFERYSE IENSNMRRIT ALRGTGLFND EWIATEKVHG ANFGIYSTDH GKAXXYAKRS GIMPPSEHFF GYHILLPDLT RYAQKGRELL TSQLKKSPSS IVINGELFGG KYDHPNLQKK RETVIVGGKP RAISAVQNDP FPQYSPSLHF YAFDMKYKLA DADTEYTTLT YDEATAIFEA IPGLLYAKAI IRGPLSKVAA FNVETFTTTI PPLVGMGDYP LRGNWAEGLV VKHCKRGKPG FAPNGHTILK FKSTAFQEIS TDRQQGPRVD EMEEVRRQSI QISGVQLPDL ESVIRDAEVR AATLHLLDHV CDNRMKSVLS KIGTEPFESQ TMTPDELATL LAKDALKDFL KEAEPKVVNS PLLLRREITR YVLFESRKYV ASKWKEIVSR QK
Validated NT Sequence
atccttctgc cgagagacga tctccttcca cttactcgca acgtacttgc gagactcgaa caagacgtaa cgcgtaatct cgcgtcgcag cagcagaggc gagttcacca ccttcggctc ggcttccttt aaaaagtcct tcagagcatc cttcgcgagc agcgtcgcca gttcatcggg cgtcatcgtc tgcgactcga aaggttccgt gccgatcttt gacagcacgc tcttcatgcg gttgtcgcag acgtggtcga gcaggtgcag cgtcgccgcc cgcacctcgg cgtcccggat gacgctctcc agatctggca gctgcacgcc ggagatttgg atcgactggc gccgcacctc ttccatctcg tccacccgcg ggccctgctg gcggtcggtg gaaatttctt gaaaggcagt ggacttgaac ttcaggatcg tgtgaccgtt gggggcgaag ccgggcttgc cgcgcttgca gtgcttcacc acgaggccct ctgcccagtt gccgcgcagc gggtagtcgc ccatgccgac gagcggtgga atcgttgtcg tgaaggtctc gacgttgaag gcggccacct tgctgagggg cccgcggatg atggccttgg cgtagagcag tccggggatg gcttcgaaaa tggcggttgc ctcgtcgtac gtcaaggtgg tgtactcggt gtctgcgtca gccagcttgt acttcatatc gaaggcgtag aagtgaagac tggggctgta ctgcggaaaa ggatcgttct gcacggcgga tatggcgcgc ggctttccac ccacgatcac cgtctctcgc ttcttctgca ggttcgggtg gtcgtacttc cccccaaaga gctcgccgtt gatcacgatg ctgctgggtg actttttcag ctggctcgtc aacaactcgc ggcccttctg cgcgtaccgc gtgaggtcgg gaagcaagat gtggtaacca aaaaagtgtt cgctgggcgg catgatgcca ctccgcttcg cgtaccnnnt cgccttgccg tggtcggtgg agtagatgcc gaagttggcg ccgtgcacct tttcagttgc gatccactcg tcgttgaaaa gtccggtgcc cctcagcgcg gtgatgcgcc gcatgttcga gttttcaatt tcgctgtaac gctcaaaaac agcatccttg ggctcatcgg cccgcaggag cggcgaccgc cggagcagac gcagcagctg gcagcgccgc aacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHML RRCQLLRLLR RSPLLRADEP KDAVFERYSE IENSNMRRIT ALRGIGLFND EWIATEKVHG ANFGIYSTDH GKAIRYAKRS GIMPPSEHFF GYHILLPDLT RYAQKGRELL TSQLKKSPSS IVINGELFGG KYDHPNLQKK RETVIVGGKP RAISAVQNDP FPQYSPSLHF YAFDMKYKLA DADTEYTTLT YDEATAIFEA IPGLLYAKAI IRGPLSKVAA FNVETFTTTI PPLVGMGDYP LRGNWAEGLV VKHCKRGKPG FAPNGHTILK FKSTAFQEIS TDRQQGPRVD EMEEVRRQSI QISGVQLPDL ESVIRDAEVR AATLHLLDHV CDNRMKSVLS KIGTEPFESQ TMTPDELATL LAKDALKDFL KEAEPKVVNS PLLLRREITR YVLFESRKYV ASKWKEIVSR QKDADSDAAQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc ggcgctgcca gctgctgcgt ctgctccggc ggtcgccgct cctgcgggcc gatgagccca aggatgctgt tttcgagcgc tacagcgaaa ttgaaaactc gaacatgcgg cgcatcaccg cgctgagagg catcggactt ttcaacgacg agtggatcgc aaccgaaaag gtgcacggcg ccaacttcgg catctactcc accgaccacg gcaaggcgat ccgttacgcg aagcggagtg gcatcatgcc gcccagcgaa cacttttttg gttaccacat cttgcttccg gacctcacgc ggtacgcgca gaagggccgc gagttgttga cgagccagct gaaaaagtcg cccagcagca tcgtgatcaa cggcgagctc tttgggggga agtacgacca cccgaacctg cagaagaagc gagagacggt gatcgtgggt ggaaagccgc gcgccatatc cgccgtgcag aacgatcctt ttccgcagta cagccccagt cttcacttct acgccttcga tatgaagtac aagctggctg acgcagacac cgagtacacc accttgacgt acgacgaggc aaccgccatt ttcgaagcca tccccggact gctctacgcc aaggccatca tccgcgggcc cctcagcaag gtggccgcct tcaacgtcga gaccttcacg acaacgattc caccgctcgt cggcatgggc gactacccgc tgcgcggcaa ctgggcagag ggcctcgtgg tgaagcactg caagcgcggc aagcccggct tcgcccccaa cggtcacacg atcctgaagt tcaagtccac tgcctttcaa gaaatttcca ccgaccgcca gcagggcccg cgggtggacg agatggaaga ggtgcggcgc cagtcgatcc aaatctccgg cgtgcagctg ccagatctgg agagcgtcat ccgggacgcc gaggtgcggg cggcgacgct gcacctgctc gaccacgtct gcgacaaccg catgaagagc gtgctgtcaa agatcggcac ggagccgttc gagtcgcaga cgatgacgcc cgatgaactg gcgacgctgc tcgcgaagga tgctctgaag gactttttaa aggaagccga gccgaaggtg gtgaactcgc ctctgctgct gcgacgcgag attacgcgtt acgtcttgtt cgagtctcgc aagtacgttg cgagtaagtg gaaggagatc gtctctcggc agaaggatgc agacagcgac gcggcacagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat