CrfaA.10252.a

threonyl-tRNA synthetase, putative

CENTER ID: CrfaA.10252.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.10252.a.B2.GE43874 TEdwards Stma structure domain 285 795
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:CFAC1_260016450

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGKKKNVET TVNLAALPQP DFWAHRVEVF ERLWEEQQNR YESMKAPIKV HLPDDKVMEA ESWVNCPLDI AKRLSNSLAE KVVVARVNDQ LWDLTRPFEG DCSLELLDWD DKEARDVFWH SSAHVLGYAM ERILDTKLSV GPALEEGGFF YEGLTNRPVS ESDYKAIESA MQELVKQKMP YQRLEVAKED ALRLFNYTEF KSKILASKVQ DGATCTVYRC GMLIDPCRGP HIPDTGRVKA FAVTKNSSSY FEGKAENEVL QRVYGISFPK NPMLVEWKKI QEEAAKRDHR VIGRQQALFN FNEVSPGSAF WLPHGARIYN AIVEHMRKQY RRRGFQEVIS PNMYSSKLWM VSGHWEKYAE NMFRTVCEKE EYGLKPMNCP GHCIMFASQQ HSYRELPIRY ADFGVLHRNE LSGALTGLTR VRRFQQDDAH IFCRMDQVTD EIEAALQFLS DVYGVMGFKF FFCHSTRPEN KLGSDAVWDQ AESQLCTALN RFCGIPEELP DPTRPGKTFH FDGRPDAVKK MKVLMKKAER GEDASAWKGP VNDGEGWTEN TGDGAFYGPK IDIVVEDALR RRHQCATIQL DFNLPNRFGL KYTLPTSEKE SAAAAAAPAE AAAGDKAKHA DPAGAASAAP AGEPAAKPKP GTYEAAVRDL GIDLTLDPNQ ARPVMIHRAI MGSLERSIAI LCEHYGGEWP FWMSPRQVMV VPVSGPCYDY AQKVRDAIFE AGFYADVDNG ASTLDKKIRN AETARYNFIL VVGQQEMDAD SVNIRARGEK RLGNKTLAEA IQWFKELADS SSREY
NT Sequence
ATGTCCGGCA AGAAGAAGAA TGTGGAGACG ACCGTGAACC TGGCCGCGCT GCCGCAGCCA GACTTCTGGG CCCACCGCGT CGAGGTCTTT GAGCGCCTCT GGGAGGAGCA GCAGAACCGC TACGAGTCGA TGAAAGCGCC AATCAAGGTG CACCTGCCCG ATGACAAGGT GATGGAGGCG GAGAGCTGGG TGAACTGCCC GCTGGACATC GCGAAGCGCC TGTCCAACTC TCTGGCCGAG AAGGTCGTGG TCGCCCGCGT GAACGACCAG CTGTGGGACC TCACTCGCCC CTTCGAGGGC GACTGCTCCC TCGAGCTGCT CGACTGGGAC GACAAGGAGG CACGCGACGT GTTCTGGCAC AGCTCGGCGC ACGTCCTCGG CTACGCCATG GAGCGCATCC TCGACACGAA GCTCTCGGTC GGCCCGGCGC TGGAGGAGGG TGGCTTCTTC TACGAGGGCC TGACCAACCG CCCGGTGTCG GAGTCCGACT ACAAGGCGAT CGAGTCGGCG ATGCAGGAGC TGGTGAAGCA GAAGATGCCC TACCAGCGCC TCGAGGTCGC CAAGGAGGAC GCCCTGCGCC TCTTCAACTA CACCGAGTTC AAGAGCAAGA TCCTCGCGAG CAAGGTGCAG GACGGCGCTA CGTGCACCGT GTACCGGTGC GGCATGCTCA TCGACCCCTG CCGCGGCCCG CACATCCCTG ACACCGGCCG CGTGAAGGCC TTCGCCGTCA CCAAGAACTC GTCGTCCTAC TTCGAGGGCA AGGCGGAGAA CGAGGTGCTG CAGCGCGTGT ACGGCATCTC CTTCCCCAAG AACCCGATGC TGGTGGAGTG GAAGAAGATC CAGGAGGAGG CGGCGAAGCG CGACCACCGC GTGATTGGCC GCCAGCAGGC GCTCTTCAAC TTCAACGAGG TGTCGCCCGG CAGCGCCTTC TGGCTGCCGC ACGGTGCGCG CATCTACAAC GCGATCGTCG AGCACATGCG TAAGCAGTAC CGCCGCCGCG GCTTCCAGGA GGTGATCTCG CCCAACATGT ACTCGTCGAA GCTGTGGATG GTGTCGGGGC ACTGGGAGAA GTACGCCGAG AACATGTTCC GCACCGTGTG CGAGAAGGAG GAGTACGGGC TGAAGCCGAT GAACTGCCCG GGCCACTGCA TCATGTTCGC CTCGCAGCAG CACTCCTACA GGGAGCTGCC GATCCGCTAC GCCGACTTTG GCGTGCTGCA CCGCAACGAG CTGAGCGGGG CCCTGACCGG CCTCACCCGC GTGCGCCGCT TCCAGCAGGA TGACGCGCAC ATCTTCTGCC GCATGGACCA GGTCACCGAC GAGATCGAGG CGGCCCTGCA GTTCCTCAGC GACGTTTACG GCGTGATGGG CTTCAAGTTC TTCTTCTGCC ACTCTACCCG CCCAGAGAAC AAGCTCGGCT CCGACGCCGT GTGGGACCAG GCGGAGTCGC AGCTGTGCAC CGCGTTGAAC CGCTTCTGCG GCATCCCGGA GGAGCTGCCC GACCCGACCC GCCCCGGCAA GACCTTCCAC TTCGACGGCA GGCCCGACGC GGTGAAGAAG ATGAAGGTGC TCATGAAGAA GGCCGAGCGC GGCGAGGACG CCAGCGCGTG GAAGGGCCCG GTGAACGACG GCGAGGGGTG GACGGAGAAC ACCGGCGACG GCGCCTTTTA TGGCCCCAAG ATCGACATCG TCGTCGAGGA CGCGCTGCGC CGCCGCCACC AGTGCGCCAC GATTCAGCTC GACTTCAACC TGCCCAACCG CTTCGGCCTC AAGTACACGC TGCCCACCTC GGAGAAGGAG AGCGCCGCCG CTGCTGCCGC TCCCGCTGAG GCGGCTGCGG GTGACAAGGC GAAGCACGCT GACCCTGCTG GTGCCGCGTC CGCTGCCCCG GCGGGCGAGC CGGCCGCGAA GCCGAAGCCC GGCACGTACG AGGCTGCGGT GCGGGACCTC GGCATCGATC TCACCCTCGA CCCGAACCAG GCCCGCCCCG TGATGATCCA CCGCGCCATC ATGGGCTCCC TCGAGCGCTC CATTGCGATT CTGTGCGAGC ACTACGGCGG CGAGTGGCCC TTCTGGATGA GCCCCCGCCA GGTGATGGTG GTGCCGGTGT CTGGCCCGTG CTACGACTAC GCGCAGAAGG TGCGCGACGC CATCTTCGAG GCCGGCTTCT ACGCCGACGT GGACAACGGT GCGTCGACCC TCGACAAGAA GATCCGCAAC GCCGAGACGG CGCGCTACAA CTTTATCCTC GTGGTGGGCC AGCAGGAGAT GGACGCCGAC AGCGTGAACA TCCGCGCGCG TGGCGAGAAG CGGCTGGGCA ACAAGACGCT GGCGGAGGCA ATTCAGTGGT TCAAGGAGCT GGCGGACTCG AGCAGCCGCG AGTACTAA
Details for CrfaA.10252.a.B2.GE43874
HARVESTED ON: 11/2/2020
SEQUENCED ON: 11/10/2020
EXPECTED MW: 58kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 84
PERCENT COVERAGE: 64
Validated AA Sequence
MAHHHHHHMA KRDHRVIGRQ QALFNFNEVS PGSAFWLPHG ARIYNAIVEH MRKQYRRRGF QEVISPNMYS SKLWMVSGHW EKYAENMFRT VCEKEEYGLK PMNCPGHCIM FASQQHSYRE LPIRYADFGV LHRNELSGAL TGLTRVRRFQ QDDAHIFCRM DQVTDEIEAA LQFLSDVYGV MGFKFFFCHS TRPENKLGSD AVWXQAESQL CTALNRFCGI PEELPDPTRP GXTFHFDGRP DAVKKMKVLM KKAERGEDAS AWXGPGERRR GVDGEHRRRR LLWPQDRHRR RGRAAPPPPV RHDSARLQPA QPLRPQVHAA HXGEGERRRC CRSR
Validated NT Sequence
atggctcacc accaccacca ccatatggcg aagcgcgacc accgcgtgat tggccgccag caggcgctct tcaacttcaa cgaggtgtcg cccggcagcg ccttctggct gccgcacggt gcgcgcatct acaacgcgat cgtcgagcac atgcgtaagc agtaccgccg ccgcggcttc caggaggtga tctcgcccaa catgtactcg tcgaagctgt ggatggtgtc ggggcactgg gagaagtacg ccgagaacat gttccgcacc gtgtgcgaga aggaggagta cgggctgaag ccgatgaact gcccgggcca ctgcatcatg ttcgcctcgc agcagcactc ctacagggag ctgccgatcc gctacgccga ctttggcgtg ctgcaccgca acgagctgag cggggccctg accggcctca cccgcgtgcg ccgcttccag caggatgacg cgcacatctt ctgccgcatg gaccaggtca ccgacgagat cgaggctgcc ctgcagttcc tcagcgacgt ctacggcgtg atgggcttca agttcttctt ctgccactcc acccgcccag agaacaagct cggctccgac gccgtgtggg ancaggcgga gtcgcagctg tgcaccgcgt tgaaccgctt ctgcggcatc ccggaggagc tgcccgaccc gacccgcccc ggnnagacct tccacttcga cggcaggccc gacgcggtga agaagatgaa ggtgctcatg aagaaggccg agcgcggcga ggacgccagc gcgtggaang ggcccggtga acgacggcga ggggtggacg gagaacaccg gcgacggcgc cttttatggc cccaagatcg acatcgtcgt cgaggacgcg ctgcgccgcc gccaccagtg cgccacgatt cagctcgact tcaacctgcc caaccgcttc ggcctcaagt acacgctgcc cacntcggag aaggagagcg ccgccgctgc tgccgctccc gctgaggcgg ctgcgggtga caaggcgaag cacgctgacc ctgctggtgc cgcgtccgct gccccggcgg gcgagccggc cgcgaagccg aagcccggca cgtacgaggc tgcggtgcgg gacctcggca tcgatctcac cctcgacccg aaccaggccc gccccgtgat gatccaccgc gccatcatgg gctccctcga gcgctccatt gcgattctgt gcgagcacta cggcggcgag tggcccttct ggatgagccc ccgccaggtg atggtggtgc cggtgtctgg cccgtgctac gactacgcgc agaaggtgcg cgacgccatc ttcgaggccg gcttctacgc cgacgtggac aacggtgcgt cgaccctcga caagaagatc cgcaacgccg agacggcgcg ctacaacttt atcctcgtgg tgggccagca ggagatggac gccgacagcg tgaacatccg cgcgcgtggc gagaagcggc tgggcaacaa gacgctggcg gaggcaattc agtggttcaa ggagctggc
Expected Protein Sequence
MAHHHHHHAK RDHRVIGRQQ ALFNFNEVSP GSAFWLPHGA RIYNAIVEHM RKQYRRRGFQ EVISPNMYSS KLWMVSGHWE KYAENMFRTV CEKEEYGLKP MNCPGHCIMF ASQQHSYREL PIRYADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCRMD QVTDEIEAAL QFLSDVYGVM GFKFFFCHST RPENKLGSDA VWDQAESQLC TALNRFCGIP EELPDPTRPG KTFHFDGRPD AVKKMKVLMK KAERGEDASA WKGPVNDGEG WTENTGDGAF YGPKIDIVVE DALRRRHQCA TIQLDFNLPN RFGLKYTLPT SEKESAAAAA APAEAAAGDK AKHADPAGAA SAAPAGEPAA KPKPGTYEAA VRDLGIDLTL DPNQARPVMI HRAIMGSLER SIAILCEHYG GEWPFWMSPR QVMVVPVSGP CYDYAQKVRD AIFEAGFYAD VDNGASTLDK KIRNAETARY NFILVVGQQE MDADSVNIRA RGEKRLGNKT LAEAIQWFKE LADSSSREY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgaagc gcgaccaccg cgtgattggc cgccagcagg cgctcttcaa cttcaacgag gtgtcgcccg gcagcgcctt ctggctgccg cacggtgcgc gcatctacaa cgcgatcgtc gagcacatgc gtaagcagta ccgccgccgc ggcttccagg aggtgatctc gcccaacatg tactcgtcga agctgtggat ggtgtcgggg cactgggaga agtacgccga gaacatgttc cgcaccgtgt gcgagaagga ggagtacggg ctgaagccga tgaactgccc gggccactgc atcatgttcg cctcgcagca gcactcctac agggagctgc cgatccgcta cgccgacttt ggcgtgctgc accgcaacga gctgagcggg gccctgaccg gcctcacccg cgtgcgccgc ttccagcagg atgacgcgca catcttctgc cgcatggacc aggtcaccga cgagatcgag gcggccctgc agttcctcag cgacgtttac ggcgtgatgg gcttcaagtt cttcttctgc cactctaccc gcccagagaa caagctcggc tccgacgccg tgtgggacca ggcggagtcg cagctgtgca ccgcgttgaa ccgcttctgc ggcatcccgg aggagctgcc cgacccgacc cgccccggca agaccttcca cttcgacggc aggcccgacg cggtgaagaa gatgaaggtg ctcatgaaga aggccgagcg cggcgaggac gccagcgcgt ggaagggccc ggtgaacgac ggcgaggggt ggacggagaa caccggcgac ggcgcctttt atggccccaa gatcgacatc gtcgtcgagg acgcgctgcg ccgccgccac cagtgcgcca cgattcagct cgacttcaac ctgcccaacc gcttcggcct caagtacacg ctgcccacct cggagaagga gagcgccgcc gctgctgccg ctcccgctga ggcggctgcg ggtgacaagg cgaagcacgc tgaccctgct ggtgccgcgt ccgctgcccc ggcgggcgag ccggccgcga agccgaagcc cggcacgtac gaggctgcgg tgcgggacct cggcatcgat ctcaccctcg acccgaacca ggcccgcccc gtgatgatcc accgcgccat catgggctcc ctcgagcgct ccattgcgat tctgtgcgag cactacggcg gcgagtggcc cttctggatg agcccccgcc aggtgatggt ggtgccggtg tctggcccgt gctacgacta cgcgcagaag gtgcgcgacg ccatcttcga ggccggcttc tacgccgacg tggacaacgg tgcgtcgacc ctcgacaaga agatccgcaa cgccgagacg gcgcgctaca actttatcct cgtggtgggc cagcaggaga tggacgccga cagcgtgaac atccgcgcgc gtggcgagaa gcggctgggc aacaagacgc tggcggaggc aattcagtgg ttcaaggagc tggcggactc gagcagccgc gagtactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t