CrfaA.01031.a
Glucose-6-phosphate 1-dehydrogenase (G6PDH), putative
| CENTER ID: | CrfaA.01031.a |
| ORGANISM: | Crithidia fasciculata Cf-Cl |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrfaA.01031.a.HZ1.PW39196 | full length | 1 | 561 |
Structures
8FUY
DEPOSITED: 1/18/2023
DETERMINATION: XRay
CLONE: CrfaA.01031.a.AE1.GE44777
PROTEIN: CrfaA.01031.a.AE1.PW39155
8U2W
DEPOSITED: 9/6/2023
DETERMINATION: XRay
CLONE: CrfaA.01031.a.HZ1.GE44778
PROTEIN: CrfaA.01031.a.HZ1.PW39196
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TriTrypDB:CFAC1_290028300 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGEHPSIDR DPYVVDLDTI LDNLREQVLD KKPDDVLKFI SKSALTLQSD EKRESCERIV SKVSEEQKRR PLTVVVLGAS GDLAKKKTFP ALFQLYCDGL LPPQINIVGY ARTKQDDVEK WKHETLTKYF SRLHERSCHV EPFLKHVTYF TGSYDKKEDF QRLDEHVSKL EDAFDGEEKA GDRLFYLALP PSAFAGACGS IRAGAMPREG GWIRVIIEKP FGHDTESSAE LSKAIEPFFD ESQIYRIDHY LGKEMVQNII TTRFANRIFS ALWNSNNIAC VQITFKETIG TEGRGGYFDS IGIIRDVMQN HLTQILALLA MEKPNSLDAE RIRDEKVSLL KCIAPIGKDD CVLGQYTASA DGSIPGYLED ETVPKGSTCP TFAVLRLHIN NDRWAGVPFI LKAGKAVEQK YVAIRIQFKD EIRPYGDAAQ RNELVIRAQP SEAMYMKITT KMPGLNEDLR ETHQTELDLT YHSRFNVHLP DAYESLISDA LRGNSTNFVR KDELDVAWRI FTPLLHQIDK GEVKPIPYQA GTRGPKEADD FILNSGFKFQ KGYHWLAPNK L
NT Sequence
atgtccggag agcaccccag catcgaccgc gacccttacg tcgtggacct ggacaccatc ctggacaacc tccgcgagca ggttctggac aagaagccgg atgacgtcct caagttcatc tcgaagagcg cactgacgct gcagagcgac gagaagcggg agagttgcga gcgcatcgtg tcgaaggtga gcgaggagca gaagcggcgc ccgctgaccg tcgtcgtgct cggcgctagc ggcgacctcg cgaagaagaa gacgttcccg gcgctcttcc agctgtactg cgacggcctg ctgcccccgc agatcaacat cgtcggctac gcccgcacga agcaggatga cgtggagaag tggaagcacg agacgctcac caagtacttc tcgcgcctcc acgagcgcag ctgccacgtg gagcccttcc tgaagcacgt cacgtacttc accggcagct acgacaagaa ggaggacttc cagaggctgg acgagcacgt cagcaagctg gaggacgcct ttgacggaga ggagaaggcg ggtgaccggc ttttctacct cgcgctgcca ccgagcgcct tcgcgggggc atgtggcagc atccgcgctg gtgcgatgcc gcgcgaaggc ggctggatcc gcgtcatcat cgagaagccc ttcggccacg acacggagag ctccgccgag ctgtccaagg cgatcgagcc cttctttgac gagtcgcaga tctaccgcat cgaccactac ctcggaaagg agatggtgca gaacatcatc accacgcgct tcgccaaccg catcttcagc gcgctgtgga actcgaacaa catcgcgtgt gtgcagatca ccttcaagga gacgatcggc acggaggggc gtggcggcta ctttgacagc atcggcatca tccgcgacgt catgcagaac caccttaccc agatcctcgc cctgctcgcg atggagaagc ccaacagcct cgacgctgag cgcatccgcg acgagaaggt gtcgctgctg aagtgcattg ccccgatcgg gaaggacgac tgcgtgctgg gccagtacac cgcctcggca gacggctcga tccccggcta cctggaggac gagacagtgc ccaagggcag cacctgcccc accttcgcgg tgctgcggct gcacatcaac aacgaccgct gggccggcgt ccccttcatc ctcaaggccg gcaaggcggt ggagcagaag tacgtggcca ttcgcatcca gttcaaggac gaaattcgcc cctacggcga cgccgcgcag cgcaacgagc ttgtcatccg tgcccagccg tcggaggcga tgtacatgaa gatcaccacc aagatgccgg gtctcaacga ggacctccgc gagacgcacc agacggagct cgacctcacc taccactccc gcttcaacgt gcacctgccg gacgcctacg agagcctcat cagcgacgcg ctgcgcggca actcgacgaa ctttgtgcgc aaggacgagc tcgatgtggc gtggcgcatc ttcacgccgc tgctgcatca gatcgacaag ggtgaggtga agccgatccc ctaccaggcc ggtacccgtg gcccgaagga ggcggacgac ttcattctca acagcggatt caagttccag aagggctacc actggctcgc accgaacaag ctgtag
Details for CrfaA.01031.a.HZ1.PW39196
| PURIFICATION DATe: | 2/23/2023 |
| CONCENTRATION: | 19.7mg/ml |
| OBSERVED MW: | 77kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | Rosetta2 (DE3) |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS GEHPSIDRDP YVVDLDTILD NLREQVLDKK PDDVLKFISK SALTLQSDEK RESCERIVSK VSEEQKRRPL TVVVLGASGD LAKKKTFPAL FQLYCDGLLP PQINIVGYAR TKQDDVEKWK HETLTKYFSR LHERSCHVEP FLKHVTYFTG SYDKKEDFQR LDEHVSKLED AFDGEEKAGD RLFYLALPPS AFAGACGSIR AGAMPREGGW IRVIIEKPFG HDTESSAELS KAIEPFFDES QIYRIDHYLG KEMVQNIITT RFANRIFSAL WNSNNIACVQ ITFKETIGTE GRGGYFDSIG IIRDVMQNHL TQILALLAME KPNSLDAERI RDEKVSLLKC IAPIGKDDCV LGQYTASADG SIPGYLEDET VPKGSTCPTF AVLRLHINND RWAGVPFILK AGKAVEQKYV AIRIQFKDEI RPYGDAAQRN ELVIRAQPSE AMYMKITTKM PGLNEDLRET HQTELDLTYH SRFNVHLPDA YESLISDALR GNSTNFVRKD ELDVAWRIFT PLLHQIDKGE VKPIPYQAGT RGPKEADDFI LNSGFKFQKG YHWLAPNKL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGAAGTCGGA CTCAGAAGTC AATCAAGAAG CTAAGCCAGA GGTCAAGCCA GAAGTCAAGC CTGAGACTCA CATCAATTTA AAGGTGTCCG ATGGATCTTC AGAGATCTTC TTCAAGATCA AAAAGACCAC TCCTTTAAGA AGGCTGATGG AAGCGTTCGC TAAAAGACAG GGTAAGGAAA TGGACTCCTT AAGATTCTTG TACGACGGTA TTAGAATTCA AGCTGATCAG ACCCCTGAAG ATTTGGACAT GGAGGATAAC GATATTATTG AGGCTCACAG AGAACAGATT GGTGGTACCC TGGAAGCTCA GACCCAGGGT CCTGGTTCGA TGTCCGGAGA GCACCCCAGC ATCGACCGCG ACCCTTACGT CGTGGACCTG GACACCATCC TGGACAACCT CCGCGAGCAG GTTCTGGACA AGAAGCCGGA TGACGTCCTC AAGTTCATCT CGAAGAGCGC ACTGACGCTG CAGAGCGACG AGAAGCGGGA GAGTTGCGAG CGCATCGTGT CGAAGGTGAG CGAGGAGCAG AAGCGGCGCC CGCTGACCGT CGTCGTGCTC GGCGCTAGCG GCGACCTCGC GAAGAAGAAG ACGTTCCCGG CGCTCTTCCA GCTGTACTGC GACGGCCTGC TGCCCCCGCA GATCAACATC GTCGGCTACG CCCGCACGAA GCAGGATGAC GTGGAGAAGT GGAAGCACGA GACGCTCACC AAGTACTTCT CGCGCCTCCA CGAGCGCAGC TGCCACGTGG AGCCCTTCCT GAAGCACGTC ACGTACTTCA CCGGCAGCTA CGACAAGAAG GAGGACTTCC AGAGGCTGGA CGAGCACGTC AGCAAGCTGG AGGACGCCTT TGACGGAGAG GAGAAGGCGG GTGACCGGCT TTTCTACCTC GCGCTGCCAC CGAGCGCCTT CGCGGGGGCA TGTGGCAGCA TCCGCGCTGG TGCGATGCCG CGCGAAGGCG GCTGGATCCG CGTCATCATC GAGAAGCCCT TCGGCCACGA CACGGAGAGC TCCGCCGAGC TGTCCAAGGC GATCGAGCCC TTCTTTGACG AGTCGCAGAT CTACCGCATC GACCACTACC TCGGAAAGGA GATGGTGCAG AACATCATCA CCACGCGCTT CGCCAACCGC ATCTTCAGCG CGCTGTGGAA CTCGAACAAC ATCGCGTGTG TGCAGATCAC CTTCAAGGAG ACGATCGGCA CGGAGGGGCG TGGCGGCTAC TTTGACAGCA TCGGCATCAT CCGCGACGTC ATGCAGAACC ACCTTACCCA GATCCTCGCC CTGCTCGCGA TGGAGAAGCC CAACAGCCTC GACGCTGAGC GCATCCGCGA CGAGAAGGTG TCGCTGCTGA AGTGCATTGC CCCGATCGGG AAGGACGACT GCGTGCTGGG CCAGTACACC GCCTCGGCAG ACGGCTCGAT CCCCGGCTAC CTGGAGGACG AGACAGTGCC CAAGGGCAGC ACCTGCCCCA CCTTCGCGGT GCTGCGGCTG CACATCAACA ACGACCGCTG GGCCGGCGTC CCCTTCATCC TCAAGGCCGG CAAGGCGGTG GAGCAGAAGT ACGTGGCCAT TCGCATCCAG TTCAAGGACG AAATTCGCCC CTACGGCGAC GCCGCGCAGC GCAACGAGCT TGTCATCCGT GCCCAGCCGT CGGAGGCGAT GTACATGAAG ATCACCACCA AGATGCCGGG TCTCAACGAG GACCTCCGCG AGACGCACCA GACGGAGCTC GACCTCACCT ACCACTCCCG CTTCAACGTG CACCTGCCGG ACGCCTACGA GAGCCTCATC AGCGACGCGC TGCGCGGCAA CTCGACGAAC TTTGTGCGCA AGGACGAGCT CGATGTGGCG TGGCGCATCT TCACGCCGCT GCTGCATCAG ATCGACAAGG GTGAGGTGAA GCCGATCCCC TACCAGGCCG GTACCCGTGG CCCGAAGGAG GCGGACGACT TCATTCTCAA CAGCGGATTC AAGTTCCAGA AGGGCTACCA CTGGCTCGCA CCGAACAAGC TGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA