CrfaA.01010.a

mitogen-activated protein kinase kinase 3, putative

CENTER ID: CrfaA.01010.a
ORGANISM: Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrfaA.01010.a.HZ2.GE43697 kinase domain 22 349
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:CFAC1_090006200

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFSALQYSL QHGSHNSHAG ESVPIGSRYT LVEKIGCGSY GDVWSANRRN GSRDVYAVKR IDKRAAGTKG LRSVMGEVET MSLLSHPNIV KLEETFQDDT YLWIVMEYLP GDSLQHRLDR ERVSEAETRR FVTQLLMAIE YVHDKGIVHR DLKPSNCLLS QNDLVVKISD FGLSVLAGNE QCLTTRCGTL HFMAPEIFLD TNYGKPVDMW AMGVIAHVMF VGDYPFKGRT EAALSRDICR GYRPSNETAS SSASRSPACS PLLQDFISQL LTFDPHRRMT ATEALKHPWI RAGMELNRVS FTSPHSSLST SSQPSSRWRA AVLAVVAAQR LIYFSKVRRL DRLGYADYPL LRNYHYLMTG LFVPRGDSLD CSHMFHARPM ALLELVAMMN TCPHLRHVDV SWNNIHSLSV IQAFLKVATR HPSLRSIDLS HNPIPAVAGR GFVRLARNPL SHITTINVGD TGIGAETIRQ INTFLREKLA PSAVSHSASL MEVPSASTSP SLGGDSLFSS PSETSHQPRS KRAFPHVGRR RSAEKKTHMP RLPPITRLPP VRQGDKTL
NT Sequence
ATGGACTTCT CAGCCCTCCA GTACAGTCTT CAACACGGGT CGCATAACAG CCACGCCGGC GAGAGTGTTC CCATCGGAAG CCGCTACACG CTCGTCGAGA AAATCGGCTG CGGGTCCTAC GGCGACGTGT GGAGCGCCAA CCGCCGCAAC GGATCGCGCG ACGTGTACGC GGTGAAGCGC ATCGACAAGC GAGCAGCCGG CACGAAAGGA CTGCGCAGCG TGATGGGAGA GGTAGAGACA ATGAGCCTTC TCTCGCACCC GAATATTGTG AAGCTGGAGG AGACATTCCA AGACGACACC TACTTGTGGA TTGTCATGGA GTATCTGCCG GGAGACTCAC TGCAGCACCG CCTCGATCGC GAGAGAGTGT CCGAAGCCGA AACACGGCGA TTCGTGACGC AGCTCTTAAT GGCGATCGAG TACGTGCACG ACAAGGGCAT TGTCCACCGC GACTTAAAGC CCAGCAACTG CCTCCTCTCG CAGAATGACC TTGTGGTGAA GATCTCCGAC TTTGGCTTGT CCGTGCTGGC CGGCAACGAG CAATGTCTGA CGACGCGGTG CGGCACGCTG CACTTCATGG CACCTGAAAT TTTTCTCGAC ACGAATTACG GCAAGCCGGT CGACATGTGG GCGATGGGCG TTATAGCACA CGTGATGTTC GTCGGCGACT ACCCATTTAA AGGCCGTACC GAGGCGGCCC TCTCGCGCGA CATCTGCCGA GGCTACCGTC CCTCCAACGA GACAGCCAGC AGCTCCGCCA GTCGCTCACC TGCCTGCTCT CCTCTCCTGC AGGACTTCAT TAGCCAGCTG CTGACGTTCG ACCCGCACCG ACGCATGACG GCCACCGAGG CGCTGAAGCA CCCGTGGATA CGCGCCGGCA TGGAGCTGAA CCGCGTGTCT TTTACCTCGC CGCACTCGTC GCTGTCGACT TCGTCGCAGC CGTCATCGCG GTGGCGGGCG GCAGTGTTGG CTGTGGTGGC TGCCCAGCGC CTTATTTACT TCTCCAAGGT GCGACGGCTA GATCGGCTCG GCTACGCCGA CTACCCGTTA CTGCGCAACT ATCATTACTT GATGACGGGT CTCTTTGTGC CGCGCGGGGA CTCGCTGGAC TGCAGCCATA TGTTTCACGC CCGCCCGATG GCGCTGCTCG AGCTCGTGGC GATGATGAAC ACCTGCCCCC ATCTCCGCCA CGTGGATGTG AGCTGGAACA ACATCCACTC CCTCTCCGTC ATACAGGCCT TTCTCAAGGT GGCCACACGG CACCCGTCGT TGCGCTCGAT CGACCTCTCT CACAATCCAA TCCCTGCTGT AGCTGGCCGC GGCTTCGTTC GCCTCGCCCG CAACCCGCTC TCGCACATCA CCACCATCAA CGTCGGCGAC ACCGGCATCG GGGCGGAGAC GATACGGCAA ATCAACACGT TTCTGCGCGA GAAGTTGGCG CCGTCTGCGG TGTCGCACAG CGCCTCGCTG ATGGAGGTGC CGAGTGCGAG TACGTCGCCG AGTCTCGGTG GCGACTCCCT CTTTTCCTCG CCAAGCGAGA CGTCACATCA GCCGCGGTCG AAGCGCGCCT TTCCGCACGT GGGGCGGCGC CGCTCAGCGG AGAAGAAGAC GCACATGCCG CGTCTGCCGC CGATAACCCG CCTACCTCCA GTGCGGCAGG GCGACAAGAC TCTCTAG
Details for CrfaA.01010.a.HZ2.GE43697
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 50kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHXHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS VPIGSRYTLV EKIGCGSYGD VWSANRRNGS RDVYAVKRID KRAAGTKGLR SVMGEVETMS LLSHPNIVKL EETFQDDTYL WIVMEYLPGD SLQHRLDRER VXEAETRRFV TQLLMAIEYV HDKGIVHRDL KPSNCLLSQN DLVVKISDFG LSVLAGNEQC LTTRCGTLHF MAPEIFLDTN YGKPVDMWAM GVIAHVMFVG DYPFKGRTEA ALSRDICRGY RPSNETASSS ASRSPACSPL LQDFISQLLT FDPHRRMTAT EALKHPWIRA GMELNRVSFT SPHSSLSTSS QPSSRWRAAV LAVVAAQRLI YFSKVRRLDR LGYAD
Validated NT Sequence
agtcggcgta gccgagccga tctagccgtc gcaccttgga gaagtaaata aggcgctggg cagccaccac agccaacact gccgcccgcc accgcgatga cggctgcgac gaagtcgaca gcgacgagtg cggcgaggta aaagacacgc ggttcagctc catgccggcg cgtatccacg ggtgcttcag cgcctcggtg gccgtcatgc gtcggtgcgg gtcgaacgtc agcagctggc taatgaagtc ctgcaggaga ggagagcagg caggtgagcg actggcggag ctgctggctg tctcgttgga gggacggtag cctcggcaga tgtcgcgcga gagggccgcc tcggtacggc ctttaaatgg gtagtcgccg acgaacatca cgtgtgctat aacgcccatc gcccacatgt cgaccggctt gccgtaattc gtgtcgagaa aaatttcagg tgccatgaag tgcagcgtgc cgcaccgcgt cgtcagacat tgctcgttgc cggccagcac ggacaagcca aagtcggaga tcttcaccac aaggtcattc tgcgagagga ggcagttgct gggctttaag tcgcggtgga caatgccctt gtcgtgcacg tactcgatcg ccattaagag ctgcgtcacg aatcgccgtg tttcggcttc ggncactctc tcgcgatcga ggcggtgctg cagtgagtct cccggcagat actccatgac aatccacaag taggtgtcgt cttggaatgt ctcctccagc ttcacaatat tcgggtgcga gagaaggctc attgtctcta cctctcccat cacgctgcgc agtcctttcg tgccggctgc tcgcttgtcg atgcgcttca ccgcgtacac gtcgcgcgat ccgttgcggc ggttggcgct ccacacgtcg ccgtaggacc cgcagccgat tttctcgacg agcgtgtagc ggcttccgat gggaacactc atcgaaccag gaccctgggt ctgagcttcc agggtaccac caatctgttc tctgtgagcc tcaataatat cgttatcctc catgtccaaa tcttcagggg tctgatcagc ttgaattcta ataccgtcgt acaagaatct taaggagtcc atttccttac cctgtctttt agcgaacgct tccatcagcc ttcttaaagg agtggtcttt ttgatcttga agaagatctc tgaagatcca tcggacacct ttaaattgat gtgagtctca ggcttgactt ctggcttgac ctctggctta gcttcttgat tgacttctga gtccgacttc atatgatggn gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSSV PIGSRYTLVE KIGCGSYGDV WSANRRNGSR DVYAVKRIDK RAAGTKGLRS VMGEVETMSL LSHPNIVKLE ETFQDDTYLW IVMEYLPGDS LQHRLDRERV SEAETRRFVT QLLMAIEYVH DKGIVHRDLK PSNCLLSQND LVVKISDFGL SVLAGNEQCL TTRCGTLHFM APEIFLDTNY GKPVDMWAMG VIAHVMFVGD YPFKGRTEAA LSRDICRGYR PSNETASSSA SRSPACSPLL QDFISQLLTF DPHRRMTATE ALKHPWIRAG MELNRVSFTS PHSSLSTSSQ PSSRWRAAVL AVVAAQRLIY FSKVRRLDRL GYADYP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga gtgttcccat cggaagccgc tacacgctcg tcgagaaaat cggctgcggg tcctacggcg acgtgtggag cgccaaccgc cgcaacggat cgcgcgacgt gtacgcggtg aagcgcatcg acaagcgagc agccggcacg aaaggactgc gcagcgtgat gggagaggta gagacaatga gccttctctc gcacccgaat attgtgaagc tggaggagac attccaagac gacacctact tgtggattgt catggagtat ctgccgggag actcactgca gcaccgcctc gatcgcgaga gagtgtccga agccgaaaca cggcgattcg tgacgcagct cttaatggcg atcgagtacg tgcacgacaa gggcattgtc caccgcgact taaagcccag caactgcctc ctctcgcaga atgaccttgt ggtgaagatc tccgactttg gcttgtccgt gctggccggc aacgagcaat gtctgacgac gcggtgcggc acgctgcact tcatggcacc tgaaattttt ctcgacacga attacggcaa gccggtcgac atgtgggcga tgggcgttat agcacacgtg atgttcgtcg gcgactaccc atttaaaggc cgtaccgagg cggccctctc gcgcgacatc tgccgaggct accgtccctc caacgagaca gccagcagct ccgccagtcg ctcacctgcc tgctctcctc tcctgcagga cttcattagc cagctgctga cgttcgaccc gcaccgacgc atgacggcca ccgaggcgct gaagcacccg tggatacgcg ccggcatgga gctgaaccgc gtgtctttta cctcgccgca ctcgtcgctg tcgacttcgt cgcagccgtc atcgcggtgg cgggcggcag tgttggctgt ggtggctgcc cagcgcctta tttacttctc caaggtgcga cggctagatc ggctcggcta cgccgactac ccgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa