CrfaA.00429.b

ATP-dependent phosphofructokinase

CENTER ID:	CrfaA.00429.b
ORGANISM:	Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrfaA.00429.b.B1.GE42061	full length		1	490

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:CFAC1_200032900

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLNIDSRQR VNSKMVRAHQ APLKTVTAAD LKVERMPGQK YMNPSKKHIA RAEYRDEVNH IMYDPRPKPG SDSEFPVSCN ELMCELAAAR KHLHFNPSET SIGIVTCGGI CPGLNDVIRS ITLSGIMAYH VKRVVGFRYG YWGLSKEGSK TAIELSRNEV RQIHRFGGTI LGSSRGPQNA KEMVDTLVRM KINILFTVGG DGTQRGALTI YEEAKRRGEN IAVFGVPKTI DNDLAFSHRT FGFQTAVEQA VLAVRAAYAE AVSLNWGVGI VKLMGRESGF IAAETAVASA QANMCLIPEN PLPKEMVLRL IERRFQQSRN CVIIVAEGFG QDWETGNGGH DASGNKKLVD IGMILKKEVE KWLKANKEKY PYGTVKYIDP SYMVRACAPS ANDALFCASL ATLAVHEAMA GATGCVISMR YNNYIVVPIK AATSVRRVVS LRGALWRQVR EITVGLGDDA ENWNKQDARR QLESLNIERE RVIAKLSSKV

NT Sequence

atgccgctca acatcgactc ccgccagcgc gtgaacagca agatggtgcg tgcccaccag gcacctctca agaccgtcac tgccgccgac ctcaaggtgg agcgcatgcc tggccagaag tacatgaacc cctccaagaa gcacatcgcc cgtgctgagt accgcgatga agtcaaccac atcatgtacg acccccgccc gaagccgggc agcgacagcg agttcccggt gtcgtgcaac gagctgatgt gcgagctggc cgccgcccgc aagcacctcc actttaaccc gtccgagacc tccatcggca tcgtcacctg cggtggcatc tgcccgggtc tgaacgacgt catccgctcc atcacgcttt ccggtatcat ggcgtaccac gtgaagcgcg tcgtgggctt ccgctacggc tactggggcc tgtcgaagga gggcagcaag acggcgatcg agttgtcccg caacgaggtg cgccagatcc accgcttcgg cggcacgatc ctcggcagct cccgcggccc gcagaacgcg aaggagatgg tggacacgct ggtgcgcatg aagatcaaca tcctgttcac tgtcggtggc gacggcacgc agcgtggtgc gctgacgatc tacgaggagg cgaagcgccg cggcgagaac attgccgtct ttggcgtgcc caagacgatc gacaacgacc tcgccttctc gcaccgcacc ttcggcttcc agacggctgt ggagcaggcg gtgcttgctg tgcgcgctgc gtacgctgag gctgtgtcgc tgaactgggg cgtgggtatt gtgaagctga tgggtcgcga gagtggcttc attgctgccg agacggctgt cgcgagtgcg caggcgaaca tgtgcctgat ccctgagaac ccgctgccga aggaaatggt gcttcgtctc atcgagcgcc gcttccagca gtcacgcaac tgtgtgatca ttgttgcgga gggctttggc caggactggg agacgggcaa cggtggccac gacgcgtcgg gcaacaagaa gcttgtggac atcggcatga tcctgaagaa ggaggtggag aagtggctga aggcgaacaa ggagaagtac ccgtacggca cggtgaagta catcgacccc tcgtacatgg tgcgtgcctg cgctccgtcg gcaaacgatg cgctcttctg cgcttctctg gcgacactcg cggtgcacga ggcgatggct ggcgcgactg gctgcgtgat ttcgatgcgc tacaacaact acattgttgt gccgatcaag gccgcaacgt ctgtgcgccg tgttgtgagc ctgcgcggtg cgctgtggcg ccaggtgcgt gagattaccg ttggcctcgg cgacgatgcg gagaactgga acaagcagga tgcccgccgc cagctggagt ctctgaacat cgagcgcgag cgcgtcattg cgaagctgtc gtcgaaggtc taa

Details for CrfaA.00429.b.B1.GE42061

HARVESTED ON:	12/5/2017
SEQUENCED ON:	12/7/2017
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMP LNIDSRQRVN SKMVRAHQAP LKTVTAADLK VERMPGQKYM NPSKKHIARA EYRDEVNHIM YDPRPKPGSD SEFPVSCNEL MCELAAARKH LHFNPSETSI GIVTCGGICP GLNDVIRSIT LSGIMAYHVK RVVGFRYGYW GLSKEGSKTA IELSRNEVRQ IHRFGGTILG SSRGPQNAKE MVDTLVRMKI NILFTVGGDG TQRGALTIYE EAKRRGENIA VFGVPKTIDN DLAFSHRTFG FQTAVEQAVL AVRAAYAEAV SLNWGVGIVK LMGRESGFIA AETAVASAQA NMCLIPENPL PKEMVLRLIE RRFQQSRNCV IIVAEGFGQD WETGNGGHDA SGNKKLVDIG MILKKEVEKW LKANKEKYPY GTVKYIDPSY MVRACAPSAN DALFCASLAT LAVHEAMAGA TGCVISMRYN NYIVVPIKAA TSVRRVVSLR GALWRQVREI TVGLGDDAEN WNKQDARRQL ESLNIERERV

Validated NT Sequence

tgacgcgctc gcgctcgatg ttcagagact ccagctggcg gcgggcatcc tgcttgttcc agttctccgc atcgtcgccg aggccaacgg taatctcacg cacctggcgc cacagcgcac cgcgcaggct cacaacacgg cgcacagacg ttgcggcctt gatcggcaca acaatgtagt tgttgtagcg catcgaaatc acgcagccag tcgcgccagc catcgcctcg tgcaccgcga gtgtcgccag agaagcgcag aagagcgcat cgtttgccga cggagcgcag gcacgcacca tgtacgaggg gtcgatgtac ttcaccgtgc cgtacgggta cttctccttg ttcgccttca gccacttctc cacctccttc ttcaggatca tgccgatgtc cacaagcttc ttgttgcccg acgcgtcgtg gccaccgttg cccgtctccc agtcctggcc aaagccctcc gcaacaatga tcacacagtt gcgtgactgc tggaagcggc gctcgatgag acgaagcacc atttccttcg gcagcgggtt ctcagggatc aggcacatgt tcgcctgcgc actcgcgaca gccgtctcgg cagcaatgaa gccactctcg cgacccatca gcttcacaat acccacgccc cagttcagcg acacagcctc agcgtacgca gcgcgcacag caagcaccgc ctgctccaca gccgtctgga agccgaaggt gcggtgcgag aaggcgaggt cgttgtcgat cgtcttgggc acgccaaaga cggcaatgtt ctcgccgcgg cgcttcgcct cctcgtagat cgtcagcgca ccacgctgcg tgccgtcgcc accgacagtg aacaggatgt tgatcttcat gcgcaccagc gtgtccacca tctccttcgc gttctgcggg ccgcgggagc tgccgaggat cgtgccgccg aagcggtgga tctggcgcac ctcgttgcgg gacaactcga tcgccgtctt gctgccctcc ttcgacaggc cccagtagcc gtagcggaag cccacgacgc gcttcacgtg gtacgccatg ataccggaaa gcgtgatgga gcggatgacg tcgttcagac ccgggcagat gccaccgcag gtgacgatgc cgatggaggt ctcggacggg ttaaagtgga ggtgcttgcg ggcggcggcc agctcgcaca tcagctcgtt gcacgacacc gggaactcgc tgtcgctgcc cggcttcggg cgggggtcgt acatgatgtg gttgacttca tcgcggtact cagcacgggc gatgtgcttc ttggaggggt tcatgtactt ctggccaggc atgcgctcca ccttgaggtc ggcggcagtg acggtcttga gaggtgcctg gtgggcacgc accatcttgc tgttcacgcg ctggcgggag tcgatgttga gcggcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc tcaacatcga ctcccgccag cgcgtgaaca gcaagatggt gcgtgcccac caggcacctc tcaagaccgt cactgccgcc gacctcaagg tggagcgcat gcctggccag aagtacatga acccctccaa gaagcacatc gcccgtgctg agtaccgcga tgaagtcaac cacatcatgt acgacccccg cccgaagccg ggcagcgaca gcgagttccc ggtgtcgtgc aacgagctga tgtgcgagct ggccgccgcc cgcaagcacc tccactttaa cccgtccgag acctccatcg gcatcgtcac ctgcggtggc atctgcccgg gtctgaacga cgtcatccgc tccatcacgc tttccggtat catggcgtac cacgtgaagc gcgtcgtggg cttccgctac ggctactggg gcctgtcgaa ggagggcagc aagacggcga tcgagttgtc ccgcaacgag gtgcgccaga tccaccgctt cggcggcacg atcctcggca gctcccgcgg cccgcagaac gcgaaggaga tggtggacac gctggtgcgc atgaagatca acatcctgtt cactgtcggt ggcgacggca cgcagcgtgg tgcgctgacg atctacgagg aggcgaagcg ccgcggcgag aacattgccg tctttggcgt gcccaagacg atcgacaacg acctcgcctt ctcgcaccgc accttcggct tccagacggc tgtggagcag gcggtgcttg ctgtgcgcgc tgcgtacgct gaggctgtgt cgctgaactg gggcgtgggt attgtgaagc tgatgggtcg cgagagtggc ttcattgctg ccgagacggc tgtcgcgagt gcgcaggcga acatgtgcct gatccctgag aacccgctgc cgaaggaaat ggtgcttcgt ctcatcgagc gccgcttcca gcagtcacgc aactgtgtga tcattgttgc ggagggcttt ggccaggact gggagacggg caacggtggc cacgacgcgt cgggcaacaa gaagcttgtg gacatcggca tgatcctgaa gaaggaggtg gagaagtggc tgaaggcgaa caaggagaag tacccgtacg gcacggtgaa gtacatcgac ccctcgtaca tggtgcgtgc ctgcgctccg tcggcaaacg atgcgctctt ctgcgcttct ctggcgacac tcgcggtgca cgaggcgatg gctggcgcga ctggctgcgt gatttcgatg cgctacaaca actacattgt tgtgccgatc aaggccgcaa cgtctgtgcg ccgtgttgtg agcctgcgcg gtgcgctgtg gcgccaggtg cgtgagatta ccgttggcct cggcgacgat gcggagaact ggaacaagca ggatgcccgc cgccagctgg agtctctgaa catcgagcgc gagcgcgtca ttgcgaagct gtcgtcgaag gtctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat