CrfaA.00429.a

ATP-dependent phosphofructokinase

CENTER ID:	CrfaA.00429.a
ORGANISM:	Crithidia fasciculata Cf-Cl
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrfaA.00429.a.B1.GE42060	full length		1	486

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:CFAC1_200033000

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSATKEADHK MVKMHGAPLS KVTPADLKVE RMPGCEFLNP SRKHIVRAEY RDSVDHIMYD PRPQKGLDTE FPVSRDKLMC ELAAARKHLH FNPSETSIGI VTCGGICPGL NDVIRSITLA ATMAYHVKRV VGFRYGYWGL SKEGSKTAIE LSRNEVRQIH RFGGTILGSS RGPQNAKEMV DTLVRMKINI LFTVGGDGTQ RGALTIYEEA KRRGENIAVF GVPKTIDNDL AFSHRTFGFQ TAVEQAVIAV RAAYAEAVSL NWGVGIVKLM GRESGFIAAE TAVASAQANI CLIPENPLPK ETVLSLIERR FQQSRNCVII VAEGFGQDWE TGNGGHDASG NKKLVDIGMI LKKEVEKWLK ANKEKYPYGT VKYIDPSYMV RACAPSSNDA LFCASLATLA VHEAMAGATG CVISMRYNNY IVVPIKAATS VRRVVSLRGS LWRQVREITV GLGDDAENWN EQDMRRQLES LNIERERIIS KLASKV

NT Sequence

atgtccgcca cgaaggaagc cgaccacaag atggtgaaga tgcacggagc tccgctgtcc aaggtgacgc ccgccgacct caaggtggag cgcatgcctg gctgcgagtt cctcaacccc tccaggaagc acatcgtgcg cgccgagtac cgcgacagcg tggaccacat catgtacgac ccccgcccgc agaagggcct cgacaccgag ttccctgtat ctcgcgataa gctgatgtgc gagctggccg ccgcccgcaa gcacctccac tttaacccgt ccgagacctc catcggcatc gtcacctgcg gcggcatctg cccaggtctg aacgacgtca tccgctccat cacgctcgct gcgacgatgg cgtaccacgt gaagcgcgtc gtgggcttcc gctatggcta ctggggcctg tcgaaggagg gcagcaagac ggcgatcgag ctgtcccgca acgaggtgcg ccagattcac cgcttcggcg gcacgatcct cggcagctcc cgcggcccgc agaacgcgaa ggagatggtg gacacgctgg tacgcatgaa gatcaacatc ctgttcaccg tcggtggcga cggcacgcag cgcggtgcgc tgacgatcta cgaggaggcg aagcgccgcg gcgagaacat tgctgtcttt ggcgtgccca agacgatcga caacgacctc gccttctcgc accgcacctt cggcttccag acggctgtgg agcaggccgt gattgccgtg cgtgctgcgt acgctgaggc tgtgtcactg aactggggcg tgggcattgt gaagctgatg ggtcgcgaga gtggcttcat tgctgctgag acggccgtcg cgagtgcgca ggcaaacatc tgcctgatcc ctgagaaccc gctgccgaag gagaccgtct tgagcttaat cgagcgccgc ttccagcagt cacgcaactg tgtgatcatt gttgcggagg gctttggcca ggactgggag acgggcaacg gcggccacga cgcgtcgggc aacaagaagc ttgtggacat cggcatgatc ctgaagaagg aggtggagaa gtggctgaag gcgaacaagg agaagtaccc gtacggcacg gtgaagtaca tcgacccctc gtacatggtg cgtgcctgcg ctccatcgtc gaacgatgcg ctcttctgcg cctctctggc gacgctcgcg gtgcacgagg cgatggctgg tgcgactggc tgtgtgatct cgatgcgtta caacaactac attgttgtgc cgatcaaggc cgccacgtct gtgcgccgtg ttgtgagcct gcgcggttca ctgtggcgcc aggtgcgcga gatcaccgtc ggcctcggcg acgatgcgga gaactggaat gagcaggaca tgcgtcgcca gctggagtct ctgaacatcg agcgggagcg catcatttca aagctcgcct ctaaggtcta a

Details for CrfaA.00429.a.B1.GE42060

HARVESTED ON:	12/5/2017
SEQUENCED ON:	12/7/2017
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS ATKEADHKMV KMHGAPLSKV TPADLKVERM PGCEFLNPSR KHIVRAEYRD SVDHIMYDPR PQKGLDTEFP VSRDKLMCEL AAARKHLHFN PSETSIGIVT CGGICPGLND VIRSITLAAT MAYHVKRVVG FRYGYWGLSK EGSKTAIELS RNEVRQIHRF GGTILGSSRG PQNAKEMVDT LVRMKINILF TVGGDGTQRG ALTIYEEAKR RGENIAVFGV PKTIDNDLAF SHRTFGFQTA VEQAVIAVRA AYAEAVSLNW GVGIVKLMGR ESGFIAAETA VASAQANICL IPENPLPKET VLSLIERRFQ QSRNCVIIVA EGFGQDWETG NGGHDASGNK KLVDIGMILK KEVEKWLKAN KEKYPYGTVK YIDPSYMVRA CAPSSNDALF CASLATLAVH EAMAGATGCV ISMRYNNYIV VPIKAATSVR RVVSLRGSLW RQVREITVGL GDDAENWNEQ DMRRQLESLN IERER

Validated NT Sequence

gatgcgctcc cgctcgatgt tcagagactc cagctggcga cgcatgtcct gctcattcca gttctccgca tcgtcgccga ggccgacggt gatctcgcgc acctggcgcc acagtgaacc gcgcaggctc acaacacggc gcacagacgt ggcggccttg atcggcacaa caatgtagtt gttgtaacgc atcgagatca cacagccagt cgcaccagcc atcgcctcgt gcaccgcgag cgtcgccaga gaggcgcaga agagcgcatc gttcgacgat ggagcgcagg cacgcaccat gtacgagggg tcgatgtact tcaccgtgcc gtacgggtac ttctccttgt tcgccttcag ccacttctcc acctccttct tcaggatcat gccgatgtcc acaagcttct tgttgcccga cgcgtcgtgg ccgccgttgc ccgtctccca gtcctggcca aagccctccg caacaatgat cacacagttg cgtgactgct ggaagcggcg ctcgattaag ctcaagacgg tctccttcgg cagcgggttc tcagggatca ggcagatgtt tgcctgcgca ctcgcgacgg ccgtctcagc agcaatgaag ccactctcgc gacccatcag cttcacaatg cccacgcccc agttcagtga cacagcctca gcgtacgcag cacgcacggc aatcacggcc tgctccacag ccgtctggaa gccgaaggtg cggtgcgaga aggcgaggtc gttgtcgatc gtcttgggca cgccaaagac agcaatgttc tcgccgcggc gcttcgcctc ctcgtagatc gtcagcgcac cgcgctgcgt gccgtcgcca ccgacggtga acaggatgtt gatcttcatg cgtaccagcg tgtccaccat ctccttcgcg ttctgcgggc cgcgggagct gccgaggatc gtgccgccga agcggtgaat ctggcgcacc tcgttgcggg acagctcgat cgccgtcttg ctgccctcct tcgacaggcc ccagtagcca tagcggaagc ccacgacgcg cttcacgtgg tacgccatcg tcgcagcgag cgtgatggag cggatgacgt cgttcagacc tgggcagatg ccgccgcagg tgacgatgcc gatggaggtc tcggacgggt taaagtggag gtgcttgcgg gcggcggcca gctcgcacat cagcttatcg cgagatacag ggaactcggt gtcgaggccc ttctgcgggc gggggtcgta catgatgtgg tccacgctgt cgcggtactc ggcgcgcacg atgtgcttcc tggaggggtt gaggaactcg cagccaggca tgcgctccac cttgaggtcg gcgggcgtca ccttggacag cggagctccg tgcatcttca ccatcttgtg gtcggcttcc ttcgtggcgg acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg ccacgaagga agccgaccac aagatggtga agatgcacgg agctccgctg tccaaggtga cgcccgccga cctcaaggtg gagcgcatgc ctggctgcga gttcctcaac ccctccagga agcacatcgt gcgcgccgag taccgcgaca gcgtggacca catcatgtac gacccccgcc cgcagaaggg cctcgacacc gagttccctg tatctcgcga taagctgatg tgcgagctgg ccgccgcccg caagcacctc cactttaacc cgtccgagac ctccatcggc atcgtcacct gcggcggcat ctgcccaggt ctgaacgacg tcatccgctc catcacgctc gctgcgacga tggcgtacca cgtgaagcgc gtcgtgggct tccgctatgg ctactggggc ctgtcgaagg agggcagcaa gacggcgatc gagctgtccc gcaacgaggt gcgccagatt caccgcttcg gcggcacgat cctcggcagc tcccgcggcc cgcagaacgc gaaggagatg gtggacacgc tggtacgcat gaagatcaac atcctgttca ccgtcggtgg cgacggcacg cagcgcggtg cgctgacgat ctacgaggag gcgaagcgcc gcggcgagaa cattgctgtc tttggcgtgc ccaagacgat cgacaacgac ctcgccttct cgcaccgcac cttcggcttc cagacggctg tggagcaggc cgtgattgcc gtgcgtgctg cgtacgctga ggctgtgtca ctgaactggg gcgtgggcat tgtgaagctg atgggtcgcg agagtggctt cattgctgct gagacggccg tcgcgagtgc gcaggcaaac atctgcctga tccctgagaa cccgctgccg aaggagaccg tcttgagctt aatcgagcgc cgcttccagc agtcacgcaa ctgtgtgatc attgttgcgg agggctttgg ccaggactgg gagacgggca acggcggcca cgacgcgtcg ggcaacaaga agcttgtgga catcggcatg atcctgaaga aggaggtgga gaagtggctg aaggcgaaca aggagaagta cccgtacggc acggtgaagt acatcgaccc ctcgtacatg gtgcgtgcct gcgctccatc gtcgaacgat gcgctcttct gcgcctctct ggcgacgctc gcggtgcacg aggcgatggc tggtgcgact ggctgtgtga tctcgatgcg ttacaacaac tacattgttg tgccgatcaa ggccgccacg tctgtgcgcc gtgttgtgag cctgcgcggt tcactgtggc gccaggtgcg cgagatcacc gtcggcctcg gcgacgatgc ggagaactgg aatgagcagg acatgcgtcg ccagctggag tctctgaaca tcgagcggga gcgcatcatt tcaaagctcg cctctaaggt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat