SSGCID: Target Page

CopoB.21146.a

Alkaline protease; subtilisin-like protease

CENTER ID:	CopoB.21146.a
ORGANISM:	Coccidioides posadasii Silveira
ASSOCIATED DISEASE:	California disease, Desert rheumatism, San Joaquin valley fever, Valley fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CopoB.21146.a.A3.GE46240	AF truncation		23	407

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CPSG_04718
UniProt:	E9D3H7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQLLNLSLFF LLPFATANPI PQDSQNIIPG QYIVTLKDGL TTAEIDAHKT WLAFTHRSNI AAKGHSGIES EGVFKHFQIH KLNMYAAGLD KKTVEELRRS PHVKSVLPDQ KIYLAEAVTQ SNAGWNLGYM SSKGQPSPSW STLTNYTYDS TAGEGVWAYV LDTGVNVNHV EFEGRAILGR NSIPNRPHED TFGHGTYVGG IIAGKTYGVA KKATVVSAKA FDGGSSSYRY ILDSYEWIVK NITDSDRKSK SVINLSISGA KYQPFDDAIE NAFQAGITTV VASGNDGRDA SQNTPASSPN AITVGALRWE NTRPGFSNYG KVVDLFAPGE LIRSGWTGGN NATRVASGTS AASPHVAGLV AYLMSIETLS SPSEVTARVL NLTIPGLVKD ARGSPNKVAY NGIQEML

NT Sequence

atgcagctcc ttaacctcag cctgttcttt ttgttgccct ttgcgacagc caaccccatt ccgcaagata gccagaacat cattcctggg caatatattg ttactttgaa agatggcctc acgacggcgg aaattgatgc tcacaagacg tggcttgctt tcacccaccg atccaacatt gcggcaaagg gacacagtgg aatagagtcc gagggagttt tcaaacactt ccagattcac aaattgaata tgtacgctgc aggcctcgac aagaagactg tagaagaact cagacgcagc ccacatgtaa aatcggttct ccctgaccaa aagatatatc tagcagaggc tgtgacacag tccaatgctg gatggaacct tggctatatg tcaagtaagg gacaaccgag tccctcatgg agtacgctca caaactatac gtatgacagt acagcaggag aaggtgtttg ggcctatgtc ctggatactg gagtcaatgt gaaccacgtt gaattcgagg gacgggcaat tctgggacgt aactctatcc caaacaggcc ccatgaagat acctttggcc atggcacata cgttgggggt atcattgccg ggaaaaccta tggcgttgcg aaaaaggcta ctgtagtctc tgcaaaggct tttgacggcg gatcgagttc ctaccgatat atcctggatt catacgagtg gattgtcaag aacattactg atagtgaccg taaatctaaa tcggtcatta acttgagcat ctccggcgct aaatatcaac cgtttgatga cgcgattgaa aatgcttttc aggcgggtat caccactgtt gttgcctctg gaaatgatgg aagagatgct tcacaaaaca ccccagcttc gtcgccgaac gcaatcactg taggggcact gcgctgggag aacaccaggc caggattttc caattatgga aaagttgttg accttttcgc accgggagag ttgatcagat cgggctggac aggcggtaat aatgcgaccc gggttgcatc cggaacttct gcagcaagtc cacacgttgc tgggcttgtt gcgtatttga tgagtattga gaccttgtct tccccttcag aggtcactgc aagagttctt aacctcacca tccccgggct ggtcaaggat gcaaggggta gtccaaacaa ggttgcctac aatggaattc aggagatgtt gtaa

Details for CopoB.21146.a.A3.GE46240

HARVESTED ON:	4/8/2026
SEQUENCED ON:	data unavailable
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SDSQNIIPGQ YIVTLKDGLT TAEIDAHKTW LAFTHRSNIA AKGHSGIESE GVFKHFQIHK LNMYAAGLDK KTVEELRRSP HVKSVLPDQK IYLAEAVTQS NAGWNLGYMS SKGQPSPSWS TLTNYTYDST AGEGVWAYVL DTGVNVNHVE FEGRAILGRN SIPNRPHEDT FGHGTYVGGI IAGKTYGVAK KATVVSAKAF DGGSSSYRYI LDSYEWIVKN ITDSDRKSKS VINLSISGAK YQPFDDAIEN AFQAGITTVV ASGNDGRDAS QNTPASSPNA ITVGALRWEN TRPGFSNYGK VVDLFAPGEL IRSGWTGGNN ATRVASGTSA ASPHVAGLVA YLMSIETLSS PSEVTARVLN LTIPGLVKDA RGSPNKVAYN GIQEML

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgga tagccagaac atcattcctg ggcaatatat tgttactttg aaagatggcc tcacgacggc ggaaattgat gctcacaaga cgtggcttgc tttcacccac cgatccaaca ttgcggcaaa gggacacagt ggaatagagt ccgagggagt tttcaaacac ttccagattc acaaattgaa tatgtacgct gcaggcctcg acaagaagac tgtagaagaa ctcagacgca gcccacatgt aaaatcggtt ctccctgacc aaaagatata tctagcagag gctgtgacac agtccaatgc tggatggaac cttggctata tgtcaagtaa gggacaaccg agtccctcat ggagtacgct cacaaactat acgtatgaca gtacagcagg agaaggtgtt tgggcctatg tcctggatac tggagtcaat gtgaaccacg ttgaattcga gggacgggca attctgggac gtaactctat cccaaacagg ccccatgaag atacctttgg ccatggcaca tacgttgggg gtatcattgc cgggaaaacc tatggcgttg cgaaaaaggc tactgtagtc tctgcaaagg cttttgacgg cggatcgagt tcctaccgat atatcctgga ttcatacgag tggattgtca agaacattac tgatagtgac cgtaaatcta aatcggtcat taacttgagc atctccggcg ctaaatatca accgtttgat gacgcgattg aaaatgcttt tcaggcgggt atcaccactg ttgttgcctc tggaaatgat ggaagagatg cttcacaaaa caccccagct tcgtcgccga acgcaatcac tgtaggggca ctgcgctggg agaacaccag gccaggattt tccaattatg gaaaagttgt tgaccttttc gcaccgggag agttgatcag atcgggctgg acaggcggta ataatgcgac ccgggttgca tccggaactt ctgcagcaag tccacacgtt gctgggcttg ttgcgtattt gatgagtatt gagaccttgt cttccccttc agaggtcact gcaagagttc ttaacctcac catccccggg ctggtcaagg atgcaagggg tagtccaaac aaggttgcct acaatggaat tcaggagatg ttgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa